Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2324 and Psest_2325 are separated by 3 nucleotides Psest_2325 and Psest_2326 are separated by 58 nucleotides
Psest_2324: Psest_2324 - hypothetical protein, at 2,477,676 to 2,479,202
_2324
Psest_2325: Psest_2325 - alpha-L-glutamate ligase-related protein, at 2,479,206 to 2,480,189
_2325
Psest_2326: Psest_2326 - aminodeoxychorismate synthase, component I, bacterial clade, at 2,480,248 to 2,481,594
_2326
Position (kb)
2479
2480
2481 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2478.220 kb on + strand, within Psest_2324 at 2478.220 kb on + strand, within Psest_2324 at 2478.305 kb on - strand, within Psest_2324 at 2478.305 kb on - strand, within Psest_2324 at 2478.577 kb on - strand, within Psest_2324 at 2478.585 kb on + strand, within Psest_2324 at 2478.615 kb on + strand, within Psest_2324 at 2478.615 kb on + strand, within Psest_2324 at 2478.646 kb on + strand, within Psest_2324 at 2478.653 kb on - strand, within Psest_2324 at 2478.801 kb on + strand, within Psest_2324 at 2478.802 kb on - strand, within Psest_2324 at 2478.802 kb on - strand, within Psest_2324 at 2478.802 kb on - strand, within Psest_2324 at 2478.896 kb on + strand, within Psest_2324 at 2478.897 kb on - strand, within Psest_2324 at 2479.080 kb on + strand at 2479.080 kb on + strand at 2479.080 kb on + strand at 2479.081 kb on - strand at 2479.081 kb on - strand at 2479.081 kb on - strand at 2479.081 kb on - strand at 2479.147 kb on - strand at 2479.281 kb on + strand at 2479.281 kb on + strand at 2479.281 kb on + strand at 2479.281 kb on + strand at 2479.282 kb on - strand at 2479.282 kb on - strand at 2479.282 kb on - strand at 2479.282 kb on - strand at 2479.282 kb on - strand at 2479.293 kb on + strand at 2479.294 kb on - strand at 2479.383 kb on + strand, within Psest_2325 at 2479.383 kb on + strand, within Psest_2325 at 2479.384 kb on - strand, within Psest_2325 at 2479.384 kb on - strand, within Psest_2325 at 2479.384 kb on - strand, within Psest_2325 at 2479.521 kb on + strand, within Psest_2325 at 2479.522 kb on - strand, within Psest_2325 at 2479.597 kb on - strand, within Psest_2325 at 2479.597 kb on - strand, within Psest_2325 at 2479.674 kb on + strand, within Psest_2325 at 2479.674 kb on + strand, within Psest_2325 at 2479.675 kb on - strand, within Psest_2325 at 2479.675 kb on - strand, within Psest_2325 at 2479.675 kb on - strand, within Psest_2325 at 2479.675 kb on - strand, within Psest_2325 at 2479.675 kb on - strand, within Psest_2325 at 2479.769 kb on + strand, within Psest_2325 at 2479.938 kb on + strand, within Psest_2325 at 2479.938 kb on + strand, within Psest_2325 at 2479.939 kb on - strand, within Psest_2325 at 2479.959 kb on + strand, within Psest_2325 at 2479.959 kb on + strand, within Psest_2325 at 2479.959 kb on + strand, within Psest_2325 at 2479.960 kb on - strand, within Psest_2325 at 2480.437 kb on - strand, within Psest_2326 at 2480.437 kb on - strand, within Psest_2326 at 2480.437 kb on - strand, within Psest_2326 at 2480.437 kb on - strand, within Psest_2326 at 2480.542 kb on + strand, within Psest_2326 at 2480.542 kb on + strand, within Psest_2326 at 2480.542 kb on + strand, within Psest_2326 at 2480.543 kb on - strand, within Psest_2326 at 2480.543 kb on - strand, within Psest_2326 at 2480.543 kb on - strand, within Psest_2326 at 2480.624 kb on - strand, within Psest_2326 at 2480.714 kb on + strand, within Psest_2326 at 2480.715 kb on - strand, within Psest_2326 at 2480.801 kb on - strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.831 kb on - strand, within Psest_2326 at 2480.831 kb on - strand, within Psest_2326 at 2480.831 kb on - strand, within Psest_2326 at 2480.831 kb on - strand, within Psest_2326 at 2480.911 kb on + strand, within Psest_2326 at 2480.911 kb on + strand, within Psest_2326 at 2480.938 kb on - strand, within Psest_2326 at 2480.944 kb on + strand, within Psest_2326 at 2480.945 kb on - strand, within Psest_2326 at 2481.075 kb on + strand, within Psest_2326 at 2481.076 kb on - strand, within Psest_2326
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 2,478,220 + Psest_2324 0.36 +2.6 2,478,220 + Psest_2324 0.36 +3.0 2,478,305 - Psest_2324 0.41 +2.5 2,478,305 - Psest_2324 0.41 +2.3 2,478,577 - Psest_2324 0.59 +1.6 2,478,585 + Psest_2324 0.60 +2.3 2,478,615 + Psest_2324 0.61 +1.8 2,478,615 + Psest_2324 0.61 +2.6 2,478,646 + Psest_2324 0.64 +2.6 2,478,653 - Psest_2324 0.64 +1.7 2,478,801 + Psest_2324 0.74 +2.2 2,478,802 - Psest_2324 0.74 +2.7 2,478,802 - Psest_2324 0.74 +2.1 2,478,802 - Psest_2324 0.74 +2.2 2,478,896 + Psest_2324 0.80 +3.2 2,478,897 - Psest_2324 0.80 +1.6 2,479,080 + +3.2 2,479,080 + +0.4 2,479,080 + +2.9 2,479,081 - -1.0 2,479,081 - +0.8 2,479,081 - +0.4 2,479,081 - +4.3 2,479,147 - +3.1 2,479,281 + +2.9 2,479,281 + +1.8 2,479,281 + +2.8 2,479,281 + +1.6 2,479,282 - +3.1 2,479,282 - +1.8 2,479,282 - +2.8 2,479,282 - +1.9 2,479,282 - +2.7 2,479,293 + +3.1 2,479,294 - +1.5 2,479,383 + Psest_2325 0.18 +2.1 2,479,383 + Psest_2325 0.18 +2.0 2,479,384 - Psest_2325 0.18 +0.3 2,479,384 - Psest_2325 0.18 +2.4 2,479,384 - Psest_2325 0.18 +2.7 2,479,521 + Psest_2325 0.32 +1.3 2,479,522 - Psest_2325 0.32 +1.9 2,479,597 - Psest_2325 0.40 +3.3 2,479,597 - Psest_2325 0.40 +2.2 2,479,674 + Psest_2325 0.48 +3.3 2,479,674 + Psest_2325 0.48 +3.0 2,479,675 - Psest_2325 0.48 +2.4 2,479,675 - Psest_2325 0.48 +1.4 2,479,675 - Psest_2325 0.48 +3.5 2,479,675 - Psest_2325 0.48 +3.3 2,479,675 - Psest_2325 0.48 +3.7 2,479,769 + Psest_2325 0.57 +2.3 2,479,938 + Psest_2325 0.74 +2.6 2,479,938 + Psest_2325 0.74 +3.3 2,479,939 - Psest_2325 0.74 +3.1 2,479,959 + Psest_2325 0.77 +2.3 2,479,959 + Psest_2325 0.77 +0.8 2,479,959 + Psest_2325 0.77 +2.2 2,479,960 - Psest_2325 0.77 +3.2 2,480,437 - Psest_2326 0.14 +0.5 2,480,437 - Psest_2326 0.14 +0.5 2,480,437 - Psest_2326 0.14 -1.8 2,480,437 - Psest_2326 0.14 +0.3 2,480,542 + Psest_2326 0.22 -1.2 2,480,542 + Psest_2326 0.22 +1.4 2,480,542 + Psest_2326 0.22 +0.8 2,480,543 - Psest_2326 0.22 +0.0 2,480,543 - Psest_2326 0.22 -1.0 2,480,543 - Psest_2326 0.22 -0.1 2,480,624 - Psest_2326 0.28 +1.9 2,480,714 + Psest_2326 0.35 +0.3 2,480,715 - Psest_2326 0.35 -0.3 2,480,801 - Psest_2326 0.41 -1.5 2,480,830 + Psest_2326 0.43 +0.4 2,480,830 + Psest_2326 0.43 -1.1 2,480,830 + Psest_2326 0.43 +0.5 2,480,830 + Psest_2326 0.43 -1.2 2,480,830 + Psest_2326 0.43 -0.5 2,480,830 + Psest_2326 0.43 +0.0 2,480,830 + Psest_2326 0.43 +1.0 2,480,830 + Psest_2326 0.43 +0.1 2,480,830 + Psest_2326 0.43 -1.4 2,480,830 + Psest_2326 0.43 -0.7 2,480,830 + Psest_2326 0.43 +2.4 2,480,830 + Psest_2326 0.43 -1.0 2,480,830 + Psest_2326 0.43 -0.5 2,480,830 + Psest_2326 0.43 -1.3 2,480,831 - Psest_2326 0.43 +0.0 2,480,831 - Psest_2326 0.43 -1.1 2,480,831 - Psest_2326 0.43 -1.1 2,480,831 - Psest_2326 0.43 -1.5 2,480,911 + Psest_2326 0.49 +1.7 2,480,911 + Psest_2326 0.49 -0.7 2,480,938 - Psest_2326 0.51 +1.3 2,480,944 + Psest_2326 0.52 +0.6 2,480,945 - Psest_2326 0.52 -2.3 2,481,075 + Psest_2326 0.61 +1.1 2,481,076 - Psest_2326 0.61 -2.1
Or see this region's nucleotide sequence