Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1146 and Psest_1147 are separated by 75 nucleotides Psest_1147 and Psest_1148 are separated by 107 nucleotides Psest_1148 and Psest_1149 are separated by 126 nucleotides
Psest_1146: Psest_1146 - Protein of unknown function, DUF481., at 1,243,811 to 1,244,815
_1146
Psest_1147: Psest_1147 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 1,244,891 to 1,245,649
_1147
Psest_1148: Psest_1148 - Putative heme iron utilization protein, at 1,245,757 to 1,246,464
_1148
Psest_1149: Psest_1149 - Protein affecting phage T7 exclusion by the F plasmid, at 1,246,591 to 1,247,049
_1149
Position (kb)
1244
1245
1246 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1243.966 kb on + strand, within Psest_1146 at 1243.966 kb on + strand, within Psest_1146 at 1243.967 kb on - strand, within Psest_1146 at 1244.207 kb on + strand, within Psest_1146 at 1244.207 kb on + strand, within Psest_1146 at 1244.300 kb on + strand, within Psest_1146 at 1244.301 kb on - strand, within Psest_1146 at 1244.301 kb on - strand, within Psest_1146 at 1244.409 kb on - strand, within Psest_1146 at 1244.409 kb on - strand, within Psest_1146 at 1244.564 kb on + strand, within Psest_1146 at 1244.564 kb on + strand, within Psest_1146 at 1244.564 kb on + strand, within Psest_1146 at 1244.589 kb on - strand, within Psest_1146 at 1244.699 kb on + strand, within Psest_1146 at 1244.787 kb on - strand at 1244.838 kb on + strand at 1244.838 kb on + strand at 1244.838 kb on + strand at 1244.838 kb on + strand at 1244.838 kb on + strand at 1244.838 kb on + strand at 1244.839 kb on - strand at 1244.839 kb on - strand at 1244.839 kb on - strand at 1244.840 kb on + strand at 1244.840 kb on + strand at 1244.840 kb on + strand at 1244.840 kb on + strand at 1244.840 kb on + strand at 1244.840 kb on + strand at 1244.840 kb on + strand at 1244.840 kb on + strand at 1244.840 kb on + strand at 1244.840 kb on + strand at 1244.840 kb on + strand at 1244.840 kb on + strand at 1244.840 kb on + strand at 1244.840 kb on + strand at 1244.841 kb on - strand at 1244.841 kb on - strand at 1244.842 kb on + strand at 1244.842 kb on + strand at 1244.842 kb on + strand at 1244.842 kb on + strand at 1244.842 kb on + strand at 1244.842 kb on + strand at 1244.842 kb on + strand at 1244.842 kb on + strand at 1244.842 kb on + strand at 1244.842 kb on + strand at 1244.842 kb on + strand at 1244.842 kb on + strand at 1244.842 kb on + strand at 1244.842 kb on + strand at 1244.842 kb on + strand at 1244.934 kb on + strand at 1244.934 kb on + strand at 1244.934 kb on + strand at 1244.934 kb on + strand at 1244.934 kb on + strand at 1244.934 kb on + strand at 1244.934 kb on + strand at 1244.934 kb on + strand at 1244.934 kb on + strand at 1244.935 kb on - strand at 1244.935 kb on - strand at 1244.937 kb on + strand at 1244.937 kb on + strand at 1244.938 kb on - strand at 1244.938 kb on - strand at 1244.955 kb on + strand at 1244.955 kb on + strand at 1244.955 kb on + strand at 1244.956 kb on - strand at 1244.956 kb on - strand at 1244.956 kb on - strand at 1244.992 kb on + strand, within Psest_1147 at 1245.066 kb on - strand, within Psest_1147 at 1245.102 kb on + strand, within Psest_1147 at 1245.166 kb on - strand, within Psest_1147 at 1245.166 kb on - strand, within Psest_1147 at 1245.181 kb on + strand, within Psest_1147 at 1245.182 kb on - strand, within Psest_1147 at 1245.474 kb on + strand, within Psest_1147 at 1245.674 kb on + strand at 1245.674 kb on + strand at 1245.674 kb on + strand at 1245.674 kb on + strand at 1245.674 kb on + strand at 1245.675 kb on - strand at 1245.675 kb on - strand at 1245.675 kb on - strand at 1245.677 kb on + strand at 1245.992 kb on + strand, within Psest_1148 at 1245.992 kb on + strand, within Psest_1148 at 1245.992 kb on + strand, within Psest_1148 at 1245.992 kb on + strand, within Psest_1148 at 1245.992 kb on + strand, within Psest_1148 at 1245.992 kb on + strand, within Psest_1148 at 1245.992 kb on + strand, within Psest_1148 at 1245.993 kb on - strand, within Psest_1148 at 1245.993 kb on - strand, within Psest_1148 at 1245.993 kb on - strand, within Psest_1148 at 1245.993 kb on - strand, within Psest_1148 at 1245.993 kb on - strand, within Psest_1148 at 1245.993 kb on - strand, within Psest_1148 at 1246.075 kb on + strand, within Psest_1148 at 1246.076 kb on - strand, within Psest_1148 at 1246.076 kb on - strand, within Psest_1148 at 1246.106 kb on - strand, within Psest_1148 at 1246.159 kb on + strand, within Psest_1148 at 1246.160 kb on - strand, within Psest_1148 at 1246.161 kb on + strand, within Psest_1148 at 1246.294 kb on + strand, within Psest_1148 at 1246.503 kb on - strand at 1246.530 kb on + strand at 1246.530 kb on + strand at 1246.530 kb on + strand at 1246.530 kb on + strand at 1246.530 kb on + strand at 1246.530 kb on + strand at 1246.551 kb on + strand at 1246.551 kb on + strand at 1246.551 kb on + strand at 1246.551 kb on + strand at 1246.551 kb on + strand at 1246.552 kb on - strand at 1246.552 kb on - strand at 1246.552 kb on - strand at 1246.639 kb on + strand, within Psest_1149 at 1246.639 kb on + strand, within Psest_1149 at 1246.639 kb on + strand, within Psest_1149 at 1246.639 kb on + strand, within Psest_1149 at 1246.639 kb on + strand, within Psest_1149
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 1,243,966 + Psest_1146 0.15 +0.4 1,243,966 + Psest_1146 0.15 +2.5 1,243,967 - Psest_1146 0.16 +3.0 1,244,207 + Psest_1146 0.39 +1.6 1,244,207 + Psest_1146 0.39 +1.8 1,244,300 + Psest_1146 0.49 +3.1 1,244,301 - Psest_1146 0.49 +2.3 1,244,301 - Psest_1146 0.49 +2.5 1,244,409 - Psest_1146 0.60 +1.7 1,244,409 - Psest_1146 0.60 +2.0 1,244,564 + Psest_1146 0.75 +1.4 1,244,564 + Psest_1146 0.75 +2.2 1,244,564 + Psest_1146 0.75 +0.8 1,244,589 - Psest_1146 0.77 +2.3 1,244,699 + Psest_1146 0.88 +1.3 1,244,787 - +1.8 1,244,838 + -1.1 1,244,838 + +0.1 1,244,838 + +2.4 1,244,838 + -1.0 1,244,838 + -0.3 1,244,838 + +0.7 1,244,839 - +2.2 1,244,839 - +0.8 1,244,839 - +1.5 1,244,840 + -0.3 1,244,840 + -0.1 1,244,840 + +1.0 1,244,840 + +1.8 1,244,840 + -0.8 1,244,840 + +0.7 1,244,840 + +0.7 1,244,840 + -0.2 1,244,840 + -2.1 1,244,840 + +1.1 1,244,840 + +0.2 1,244,840 + -2.4 1,244,840 + +1.5 1,244,840 + -0.2 1,244,841 - -1.4 1,244,841 - +1.2 1,244,842 + -0.0 1,244,842 + -0.4 1,244,842 + -0.5 1,244,842 + +0.1 1,244,842 + -1.5 1,244,842 + -0.3 1,244,842 + -1.2 1,244,842 + +0.2 1,244,842 + -0.7 1,244,842 + +0.3 1,244,842 + +0.4 1,244,842 + +0.4 1,244,842 + -0.8 1,244,842 + +0.9 1,244,842 + +1.4 1,244,934 + +3.1 1,244,934 + -0.6 1,244,934 + -0.6 1,244,934 + +1.3 1,244,934 + +0.1 1,244,934 + +1.6 1,244,934 + -2.9 1,244,934 + +0.1 1,244,934 + +0.1 1,244,935 - +1.4 1,244,935 - -0.0 1,244,937 + +0.1 1,244,937 + +1.1 1,244,938 - +0.8 1,244,938 - -0.3 1,244,955 + +0.7 1,244,955 + -1.9 1,244,955 + +0.4 1,244,956 - -0.9 1,244,956 - -1.1 1,244,956 - +0.7 1,244,992 + Psest_1147 0.13 -1.1 1,245,066 - Psest_1147 0.23 +0.5 1,245,102 + Psest_1147 0.28 +1.8 1,245,166 - Psest_1147 0.36 +1.1 1,245,166 - Psest_1147 0.36 -0.5 1,245,181 + Psest_1147 0.38 +1.6 1,245,182 - Psest_1147 0.38 +0.0 1,245,474 + Psest_1147 0.77 -1.6 1,245,674 + +0.5 1,245,674 + +1.5 1,245,674 + -2.1 1,245,674 + +0.8 1,245,674 + +0.4 1,245,675 - -0.0 1,245,675 - +1.0 1,245,675 - -0.0 1,245,677 + -0.0 1,245,992 + Psest_1148 0.33 -0.1 1,245,992 + Psest_1148 0.33 -0.4 1,245,992 + Psest_1148 0.33 +1.6 1,245,992 + Psest_1148 0.33 +4.2 1,245,992 + Psest_1148 0.33 +1.8 1,245,992 + Psest_1148 0.33 +0.9 1,245,992 + Psest_1148 0.33 +2.3 1,245,993 - Psest_1148 0.33 +0.1 1,245,993 - Psest_1148 0.33 -0.1 1,245,993 - Psest_1148 0.33 +1.4 1,245,993 - Psest_1148 0.33 +0.4 1,245,993 - Psest_1148 0.33 -0.0 1,245,993 - Psest_1148 0.33 +0.5 1,246,075 + Psest_1148 0.45 +2.5 1,246,076 - Psest_1148 0.45 +0.1 1,246,076 - Psest_1148 0.45 -0.3 1,246,106 - Psest_1148 0.49 +0.9 1,246,159 + Psest_1148 0.57 +1.4 1,246,160 - Psest_1148 0.57 +0.1 1,246,161 + Psest_1148 0.57 -0.9 1,246,294 + Psest_1148 0.76 +1.1 1,246,503 - +0.4 1,246,530 + +2.3 1,246,530 + +0.6 1,246,530 + +1.3 1,246,530 + -1.2 1,246,530 + +1.1 1,246,530 + -0.6 1,246,551 + -2.8 1,246,551 + -0.3 1,246,551 + +2.2 1,246,551 + -1.6 1,246,551 + -1.2 1,246,552 - -2.2 1,246,552 - -1.4 1,246,552 - -3.3 1,246,639 + Psest_1149 0.10 -2.2 1,246,639 + Psest_1149 0.10 -0.8 1,246,639 + Psest_1149 0.10 -3.1 1,246,639 + Psest_1149 0.10 -4.1 1,246,639 + Psest_1149 0.10 -0.9
Or see this region's nucleotide sequence