Experiment: Phosphomycin 0.012 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2991 and Psest_2992 are separated by 72 nucleotides Psest_2992 and Psest_2993 are separated by 112 nucleotides Psest_2993 and Psest_2994 are separated by 90 nucleotides Psest_2994 and Psest_2995 overlap by 4 nucleotides
Psest_2991: Psest_2991 - hypothetical protein, at 3,182,268 to 3,183,488
_2991
Psest_2992: Psest_2992 - Uncharacterized conserved protein, at 3,183,561 to 3,184,010
_2992
Psest_2993: Psest_2993 - hypothetical protein, at 3,184,123 to 3,184,527
_2993
Psest_2994: Psest_2994 - Uncharacterized protein conserved in bacteria, at 3,184,618 to 3,184,911
_2994
Psest_2995: Psest_2995 - ribonuclease D, at 3,184,908 to 3,186,032
_2995
Position (kb)
3183
3184
3185 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3182.590 kb on + strand, within Psest_2991 at 3182.590 kb on + strand, within Psest_2991 at 3182.590 kb on + strand, within Psest_2991 at 3182.590 kb on + strand, within Psest_2991 at 3182.590 kb on + strand, within Psest_2991 at 3182.590 kb on + strand, within Psest_2991 at 3182.590 kb on + strand, within Psest_2991 at 3182.591 kb on - strand, within Psest_2991 at 3182.722 kb on + strand, within Psest_2991 at 3182.722 kb on + strand, within Psest_2991 at 3182.723 kb on - strand, within Psest_2991 at 3182.723 kb on - strand, within Psest_2991 at 3182.894 kb on + strand, within Psest_2991 at 3183.295 kb on + strand, within Psest_2991 at 3183.296 kb on - strand, within Psest_2991 at 3183.298 kb on + strand, within Psest_2991 at 3183.526 kb on + strand at 3183.526 kb on + strand at 3183.526 kb on + strand at 3183.527 kb on - strand at 3183.527 kb on - strand at 3183.556 kb on + strand at 3183.556 kb on + strand at 3183.556 kb on + strand at 3183.557 kb on - strand at 3183.557 kb on - strand at 3183.557 kb on - strand at 3183.557 kb on - strand at 3183.557 kb on - strand at 3183.707 kb on + strand, within Psest_2992 at 3183.872 kb on - strand, within Psest_2992 at 3183.872 kb on - strand, within Psest_2992 at 3183.872 kb on - strand, within Psest_2992 at 3183.874 kb on + strand, within Psest_2992 at 3183.874 kb on + strand, within Psest_2992 at 3183.874 kb on + strand, within Psest_2992 at 3183.874 kb on + strand, within Psest_2992 at 3183.874 kb on + strand, within Psest_2992 at 3183.874 kb on + strand, within Psest_2992 at 3183.875 kb on - strand, within Psest_2992 at 3183.875 kb on - strand, within Psest_2992 at 3183.875 kb on - strand, within Psest_2992 at 3184.016 kb on + strand at 3184.016 kb on + strand at 3184.016 kb on + strand at 3184.016 kb on + strand at 3184.017 kb on - strand at 3184.017 kb on - strand at 3184.489 kb on + strand at 3184.541 kb on - strand at 3184.564 kb on + strand at 3184.583 kb on - strand at 3184.667 kb on + strand, within Psest_2994 at 3184.667 kb on + strand, within Psest_2994 at 3184.667 kb on + strand, within Psest_2994 at 3184.667 kb on + strand, within Psest_2994 at 3184.667 kb on + strand, within Psest_2994 at 3184.667 kb on + strand, within Psest_2994 at 3184.667 kb on + strand, within Psest_2994 at 3184.668 kb on - strand, within Psest_2994 at 3184.668 kb on - strand, within Psest_2994 at 3184.668 kb on - strand, within Psest_2994 at 3184.668 kb on - strand, within Psest_2994 at 3184.668 kb on - strand, within Psest_2994 at 3184.668 kb on - strand, within Psest_2994 at 3184.856 kb on + strand, within Psest_2994 at 3184.857 kb on - strand, within Psest_2994 at 3184.857 kb on - strand, within Psest_2994 at 3184.857 kb on - strand, within Psest_2994 at 3184.862 kb on + strand, within Psest_2994 at 3184.862 kb on + strand, within Psest_2994 at 3184.862 kb on + strand, within Psest_2994 at 3184.862 kb on + strand, within Psest_2994 at 3184.862 kb on + strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.863 kb on - strand, within Psest_2994 at 3184.888 kb on - strand at 3184.888 kb on - strand at 3184.889 kb on + strand at 3184.889 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.012 mg/ml remove 3,182,590 + Psest_2991 0.26 +0.5 3,182,590 + Psest_2991 0.26 +0.7 3,182,590 + Psest_2991 0.26 -0.8 3,182,590 + Psest_2991 0.26 +1.1 3,182,590 + Psest_2991 0.26 +0.3 3,182,590 + Psest_2991 0.26 -0.6 3,182,590 + Psest_2991 0.26 -0.3 3,182,591 - Psest_2991 0.26 +1.2 3,182,722 + Psest_2991 0.37 +3.3 3,182,722 + Psest_2991 0.37 -1.4 3,182,723 - Psest_2991 0.37 -0.3 3,182,723 - Psest_2991 0.37 +0.5 3,182,894 + Psest_2991 0.51 +0.2 3,183,295 + Psest_2991 0.84 -0.5 3,183,296 - Psest_2991 0.84 -1.4 3,183,298 + Psest_2991 0.84 +0.5 3,183,526 + -2.0 3,183,526 + +1.5 3,183,526 + -1.4 3,183,527 - -0.3 3,183,527 - -1.3 3,183,556 + +0.1 3,183,556 + -2.2 3,183,556 + -1.3 3,183,557 - +0.7 3,183,557 - -1.8 3,183,557 - -0.1 3,183,557 - +0.3 3,183,557 - -0.1 3,183,707 + Psest_2992 0.32 +0.2 3,183,872 - Psest_2992 0.69 +0.7 3,183,872 - Psest_2992 0.69 -1.3 3,183,872 - Psest_2992 0.69 -0.9 3,183,874 + Psest_2992 0.70 -1.0 3,183,874 + Psest_2992 0.70 -0.9 3,183,874 + Psest_2992 0.70 -2.0 3,183,874 + Psest_2992 0.70 -0.3 3,183,874 + Psest_2992 0.70 -2.7 3,183,874 + Psest_2992 0.70 -0.1 3,183,875 - Psest_2992 0.70 -0.9 3,183,875 - Psest_2992 0.70 -0.1 3,183,875 - Psest_2992 0.70 +0.4 3,184,016 + -0.2 3,184,016 + -0.2 3,184,016 + -2.7 3,184,016 + -2.1 3,184,017 - +0.0 3,184,017 - +0.2 3,184,489 + -1.1 3,184,541 - -0.5 3,184,564 + +0.3 3,184,583 - -0.5 3,184,667 + Psest_2994 0.17 -0.6 3,184,667 + Psest_2994 0.17 +0.9 3,184,667 + Psest_2994 0.17 -0.6 3,184,667 + Psest_2994 0.17 -0.1 3,184,667 + Psest_2994 0.17 +1.4 3,184,667 + Psest_2994 0.17 +0.3 3,184,667 + Psest_2994 0.17 -0.5 3,184,668 - Psest_2994 0.17 -0.4 3,184,668 - Psest_2994 0.17 -0.8 3,184,668 - Psest_2994 0.17 +0.5 3,184,668 - Psest_2994 0.17 -0.5 3,184,668 - Psest_2994 0.17 -0.3 3,184,668 - Psest_2994 0.17 +0.3 3,184,856 + Psest_2994 0.81 +0.7 3,184,857 - Psest_2994 0.81 +1.4 3,184,857 - Psest_2994 0.81 -0.4 3,184,857 - Psest_2994 0.81 -0.3 3,184,862 + Psest_2994 0.83 +1.3 3,184,862 + Psest_2994 0.83 -0.8 3,184,862 + Psest_2994 0.83 +0.2 3,184,862 + Psest_2994 0.83 +0.1 3,184,862 + Psest_2994 0.83 +0.9 3,184,863 - Psest_2994 0.83 -0.1 3,184,863 - Psest_2994 0.83 +2.3 3,184,863 - Psest_2994 0.83 +0.7 3,184,863 - Psest_2994 0.83 +0.6 3,184,863 - Psest_2994 0.83 +0.8 3,184,863 - Psest_2994 0.83 -1.7 3,184,863 - Psest_2994 0.83 +1.4 3,184,863 - Psest_2994 0.83 -0.2 3,184,863 - Psest_2994 0.83 +0.4 3,184,863 - Psest_2994 0.83 -0.7 3,184,863 - Psest_2994 0.83 +0.3 3,184,863 - Psest_2994 0.83 -0.0 3,184,888 - -0.8 3,184,888 - +0.5 3,184,889 + +1.1 3,184,889 + +0.0
Or see this region's nucleotide sequence