Experiment: Phosphomycin 0.012 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0185 and Psest_0186 are separated by 183 nucleotides Psest_0186 and Psest_0187 are separated by 6 nucleotides Psest_0187 and Psest_0188 overlap by 4 nucleotides
Psest_0185: Psest_0185 - Cytochrome c553, at 180,926 to 181,558
_0185
Psest_0186: Psest_0186 - Protein-disulfide isomerase, at 181,742 to 182,371
_0186
Psest_0187: Psest_0187 - Metal-dependent hydrolase, at 182,378 to 183,247
_0187
Psest_0188: Psest_0188 - diguanylate cyclase (GGDEF) domain, at 183,244 to 185,142
_0188
Position (kb) 181
182
183 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 180.773 kb on + strand at 180.843 kb on + strand at 180.844 kb on - strand at 180.911 kb on + strand at 180.911 kb on + strand at 180.920 kb on + strand at 180.921 kb on - strand at 180.936 kb on + strand at 181.418 kb on + strand, within Psest_0185 at 181.419 kb on - strand, within Psest_0185 at 181.538 kb on + strand at 181.569 kb on - strand at 181.598 kb on + strand at 181.599 kb on - strand at 181.599 kb on - strand at 181.736 kb on + strand at 181.736 kb on + strand at 181.736 kb on + strand at 181.736 kb on + strand at 181.736 kb on + strand at 181.736 kb on + strand at 181.736 kb on + strand at 181.736 kb on + strand at 181.832 kb on + strand, within Psest_0186 at 181.833 kb on - strand, within Psest_0186 at 181.833 kb on - strand, within Psest_0186 at 181.833 kb on - strand, within Psest_0186 at 181.833 kb on - strand, within Psest_0186 at 182.016 kb on + strand, within Psest_0186 at 182.016 kb on + strand, within Psest_0186 at 182.017 kb on - strand, within Psest_0186 at 182.017 kb on - strand, within Psest_0186 at 182.017 kb on - strand, within Psest_0186 at 182.183 kb on + strand, within Psest_0186 at 182.184 kb on - strand, within Psest_0186 at 182.184 kb on - strand, within Psest_0186 at 182.211 kb on + strand, within Psest_0186 at 182.240 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.534 kb on + strand, within Psest_0187 at 182.534 kb on + strand, within Psest_0187 at 182.618 kb on + strand, within Psest_0187 at 182.618 kb on + strand, within Psest_0187 at 182.618 kb on + strand, within Psest_0187 at 182.618 kb on + strand, within Psest_0187 at 182.619 kb on - strand, within Psest_0187 at 182.721 kb on - strand, within Psest_0187 at 182.933 kb on + strand, within Psest_0187 at 182.990 kb on - strand, within Psest_0187 at 183.277 kb on + strand at 183.278 kb on - strand at 183.341 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.012 mg/ml remove 180,773 + -0.3 180,843 + +0.3 180,844 - +1.3 180,911 + +1.2 180,911 + +2.7 180,920 + +0.0 180,921 - +2.0 180,936 + +1.2 181,418 + Psest_0185 0.78 +1.7 181,419 - Psest_0185 0.78 +3.2 181,538 + +1.7 181,569 - -0.2 181,598 + -0.7 181,599 - -0.1 181,599 - -0.7 181,736 + -1.2 181,736 + -0.7 181,736 + -0.1 181,736 + +0.3 181,736 + -1.1 181,736 + -0.7 181,736 + -1.0 181,736 + -2.5 181,832 + Psest_0186 0.14 -1.3 181,833 - Psest_0186 0.14 -2.2 181,833 - Psest_0186 0.14 -1.6 181,833 - Psest_0186 0.14 -2.3 181,833 - Psest_0186 0.14 +0.2 182,016 + Psest_0186 0.43 -0.5 182,016 + Psest_0186 0.43 -1.9 182,017 - Psest_0186 0.44 -1.7 182,017 - Psest_0186 0.44 -1.5 182,017 - Psest_0186 0.44 -2.0 182,183 + Psest_0186 0.70 -1.5 182,184 - Psest_0186 0.70 -1.5 182,184 - Psest_0186 0.70 +0.2 182,211 + Psest_0186 0.74 -2.3 182,240 + Psest_0186 0.79 -1.3 182,282 + Psest_0186 0.86 -2.8 182,282 + Psest_0186 0.86 -1.0 182,282 + Psest_0186 0.86 -1.5 182,282 + Psest_0186 0.86 -0.5 182,282 + Psest_0186 0.86 -2.4 182,534 + Psest_0187 0.18 -0.9 182,534 + Psest_0187 0.18 +0.0 182,618 + Psest_0187 0.28 -1.5 182,618 + Psest_0187 0.28 -1.4 182,618 + Psest_0187 0.28 -2.1 182,618 + Psest_0187 0.28 +0.7 182,619 - Psest_0187 0.28 -1.2 182,721 - Psest_0187 0.39 +1.0 182,933 + Psest_0187 0.64 -3.2 182,990 - Psest_0187 0.70 -3.3 183,277 + -0.0 183,278 - -0.8 183,341 + +0.3
Or see this region's nucleotide sequence