Experiment: Phosphomycin 0.012 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0100 and Psest_0101 are separated by 275 nucleotides Psest_0101 and Psest_0102 are separated by 173 nucleotides
Psest_0100: Psest_0100 - phosphonate ABC transporter, ATP-binding protein, at 99,306 to 100,136
_0100
Psest_0101: Psest_0101 - Short-chain alcohol dehydrogenase of unknown specificity, at 100,412 to 101,176
_0101
Psest_0102: Psest_0102 - malonate decarboxylase, alpha subunit, at 101,350 to 103,017
_0102
Position (kb)
100
101
102 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6 at 99.481 kb on + strand, within Psest_0100 at 99.493 kb on + strand, within Psest_0100 at 99.493 kb on + strand, within Psest_0100 at 99.868 kb on + strand, within Psest_0100 at 99.869 kb on - strand, within Psest_0100 at 99.869 kb on - strand, within Psest_0100 at 99.894 kb on + strand, within Psest_0100 at 99.894 kb on + strand, within Psest_0100 at 99.894 kb on + strand, within Psest_0100 at 99.894 kb on + strand, within Psest_0100 at 99.895 kb on - strand, within Psest_0100 at 99.895 kb on - strand, within Psest_0100 at 99.895 kb on - strand, within Psest_0100 at 100.185 kb on - strand at 100.196 kb on + strand at 100.196 kb on + strand at 100.221 kb on + strand at 100.221 kb on + strand at 100.336 kb on + strand at 100.428 kb on + strand at 100.428 kb on + strand at 100.428 kb on + strand at 100.429 kb on - strand at 100.429 kb on - strand at 100.429 kb on - strand at 100.868 kb on + strand, within Psest_0101 at 100.907 kb on - strand, within Psest_0101 at 101.078 kb on + strand, within Psest_0101 at 101.078 kb on + strand, within Psest_0101 at 101.078 kb on + strand, within Psest_0101 at 101.078 kb on + strand, within Psest_0101 at 101.078 kb on + strand, within Psest_0101 at 101.079 kb on - strand, within Psest_0101 at 101.079 kb on - strand, within Psest_0101 at 101.079 kb on - strand, within Psest_0101 at 101.174 kb on + strand at 101.174 kb on + strand at 101.175 kb on - strand at 101.175 kb on - strand at 101.175 kb on - strand at 101.175 kb on - strand at 101.175 kb on - strand at 101.175 kb on - strand at 101.175 kb on - strand at 101.175 kb on - strand at 101.175 kb on - strand at 101.175 kb on - strand at 101.187 kb on + strand at 101.248 kb on - strand at 101.311 kb on + strand at 101.311 kb on + strand at 101.312 kb on - strand at 101.312 kb on - strand at 101.312 kb on - strand at 101.312 kb on - strand at 101.312 kb on - strand at 101.312 kb on - strand at 101.749 kb on + strand, within Psest_0102 at 101.749 kb on + strand, within Psest_0102 at 101.749 kb on + strand, within Psest_0102 at 101.749 kb on + strand, within Psest_0102 at 101.749 kb on + strand, within Psest_0102 at 101.749 kb on + strand, within Psest_0102 at 101.749 kb on + strand, within Psest_0102 at 101.750 kb on - strand, within Psest_0102 at 101.750 kb on - strand, within Psest_0102 at 101.750 kb on - strand, within Psest_0102 at 101.750 kb on - strand, within Psest_0102 at 101.750 kb on - strand, within Psest_0102 at 101.750 kb on - strand, within Psest_0102 at 101.750 kb on - strand, within Psest_0102 at 101.750 kb on - strand, within Psest_0102 at 101.750 kb on - strand, within Psest_0102 at 101.750 kb on - strand, within Psest_0102 at 101.750 kb on - strand, within Psest_0102 at 101.750 kb on - strand, within Psest_0102 at 101.761 kb on + strand, within Psest_0102 at 101.761 kb on + strand, within Psest_0102 at 101.761 kb on + strand, within Psest_0102 at 101.762 kb on - strand, within Psest_0102 at 101.839 kb on + strand, within Psest_0102 at 101.839 kb on + strand, within Psest_0102 at 101.839 kb on + strand, within Psest_0102 at 101.839 kb on + strand, within Psest_0102 at 101.839 kb on + strand, within Psest_0102 at 101.839 kb on + strand, within Psest_0102 at 101.839 kb on + strand, within Psest_0102 at 101.839 kb on + strand, within Psest_0102 at 101.839 kb on + strand, within Psest_0102 at 101.840 kb on - strand, within Psest_0102 at 101.840 kb on - strand, within Psest_0102 at 101.840 kb on - strand, within Psest_0102 at 101.840 kb on - strand, within Psest_0102 at 101.840 kb on - strand, within Psest_0102 at 101.840 kb on - strand, within Psest_0102 at 102.001 kb on + strand, within Psest_0102 at 102.001 kb on + strand, within Psest_0102 at 102.085 kb on + strand, within Psest_0102 at 102.085 kb on + strand, within Psest_0102 at 102.085 kb on + strand, within Psest_0102 at 102.085 kb on + strand, within Psest_0102 at 102.085 kb on + strand, within Psest_0102 at 102.086 kb on - strand, within Psest_0102 at 102.086 kb on - strand, within Psest_0102
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.012 mg/ml remove 99,481 + Psest_0100 0.21 +5.2 99,493 + Psest_0100 0.23 +5.7 99,493 + Psest_0100 0.23 +3.4 99,868 + Psest_0100 0.68 +1.5 99,869 - Psest_0100 0.68 +6.0 99,869 - Psest_0100 0.68 +3.4 99,894 + Psest_0100 0.71 +4.7 99,894 + Psest_0100 0.71 +2.5 99,894 + Psest_0100 0.71 +3.7 99,894 + Psest_0100 0.71 +5.4 99,895 - Psest_0100 0.71 +3.8 99,895 - Psest_0100 0.71 +2.8 99,895 - Psest_0100 0.71 +4.0 100,185 - +3.9 100,196 + +4.8 100,196 + +4.0 100,221 + +3.8 100,221 + +2.3 100,336 + +0.4 100,428 + -0.4 100,428 + +0.6 100,428 + -0.7 100,429 - +5.0 100,429 - +3.8 100,429 - +3.8 100,868 + Psest_0101 0.60 +0.7 100,907 - Psest_0101 0.65 +1.0 101,078 + Psest_0101 0.87 +1.7 101,078 + Psest_0101 0.87 -0.3 101,078 + Psest_0101 0.87 +0.1 101,078 + Psest_0101 0.87 -1.3 101,078 + Psest_0101 0.87 +0.3 101,079 - Psest_0101 0.87 +1.6 101,079 - Psest_0101 0.87 +3.5 101,079 - Psest_0101 0.87 +1.6 101,174 + +0.4 101,174 + -0.3 101,175 - +0.9 101,175 - +1.7 101,175 - +1.8 101,175 - +1.5 101,175 - +1.3 101,175 - +0.2 101,175 - +0.0 101,175 - +2.9 101,175 - +2.0 101,175 - +1.5 101,187 + -0.8 101,248 - +1.8 101,311 + -0.8 101,311 + +0.1 101,312 - +0.7 101,312 - +3.0 101,312 - -0.8 101,312 - +2.1 101,312 - -1.7 101,312 - +1.2 101,749 + Psest_0102 0.24 +0.5 101,749 + Psest_0102 0.24 +0.8 101,749 + Psest_0102 0.24 -1.3 101,749 + Psest_0102 0.24 -0.2 101,749 + Psest_0102 0.24 -0.5 101,749 + Psest_0102 0.24 -0.1 101,749 + Psest_0102 0.24 -1.0 101,750 - Psest_0102 0.24 -0.7 101,750 - Psest_0102 0.24 -0.8 101,750 - Psest_0102 0.24 +0.7 101,750 - Psest_0102 0.24 +0.6 101,750 - Psest_0102 0.24 +0.6 101,750 - Psest_0102 0.24 +0.3 101,750 - Psest_0102 0.24 -0.1 101,750 - Psest_0102 0.24 -0.5 101,750 - Psest_0102 0.24 -0.5 101,750 - Psest_0102 0.24 +0.9 101,750 - Psest_0102 0.24 +1.0 101,750 - Psest_0102 0.24 -0.6 101,761 + Psest_0102 0.25 -1.3 101,761 + Psest_0102 0.25 -0.6 101,761 + Psest_0102 0.25 +1.8 101,762 - Psest_0102 0.25 +1.4 101,839 + Psest_0102 0.29 +0.4 101,839 + Psest_0102 0.29 -0.8 101,839 + Psest_0102 0.29 +0.1 101,839 + Psest_0102 0.29 +0.9 101,839 + Psest_0102 0.29 +0.6 101,839 + Psest_0102 0.29 +1.2 101,839 + Psest_0102 0.29 -0.2 101,839 + Psest_0102 0.29 +1.0 101,839 + Psest_0102 0.29 +0.6 101,840 - Psest_0102 0.29 -0.3 101,840 - Psest_0102 0.29 +0.7 101,840 - Psest_0102 0.29 +0.3 101,840 - Psest_0102 0.29 +0.2 101,840 - Psest_0102 0.29 +2.8 101,840 - Psest_0102 0.29 +0.3 102,001 + Psest_0102 0.39 -0.0 102,001 + Psest_0102 0.39 +0.5 102,085 + Psest_0102 0.44 +1.1 102,085 + Psest_0102 0.44 +2.1 102,085 + Psest_0102 0.44 -0.3 102,085 + Psest_0102 0.44 -0.2 102,085 + Psest_0102 0.44 +0.6 102,086 - Psest_0102 0.44 +1.4 102,086 - Psest_0102 0.44 +0.5
Or see this region's nucleotide sequence