Experiment: Phosphomycin 0.012 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3125 and Psest_3126 are separated by 74 nucleotides Psest_3126 and Psest_3127 are separated by 112 nucleotides Psest_3127 and Psest_3128 are separated by 101 nucleotides
Psest_3125: Psest_3125 - threonine synthase, at 3,301,457 to 3,302,866
_3125
Psest_3126: Psest_3126 - Homoserine dehydrogenase, at 3,302,941 to 3,304,245
_3126
Psest_3127: Psest_3127 - Uncharacterized conserved protein, at 3,304,358 to 3,304,846
_3127
Psest_3128: Psest_3128 - Protein-disulfide isomerase, at 3,304,948 to 3,305,673
_3128
Position (kb)
3302
3303
3304
3305 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3302.943 kb on - strand at 3302.943 kb on - strand at 3302.943 kb on - strand at 3304.326 kb on - strand at 3304.486 kb on + strand, within Psest_3127 at 3304.487 kb on - strand, within Psest_3127 at 3304.487 kb on - strand, within Psest_3127 at 3304.487 kb on - strand, within Psest_3127 at 3304.487 kb on - strand, within Psest_3127 at 3304.487 kb on - strand, within Psest_3127 at 3304.487 kb on - strand, within Psest_3127 at 3304.555 kb on + strand, within Psest_3127 at 3304.556 kb on - strand, within Psest_3127 at 3304.556 kb on - strand, within Psest_3127 at 3304.660 kb on - strand, within Psest_3127 at 3304.748 kb on - strand, within Psest_3127 at 3304.764 kb on + strand, within Psest_3127 at 3304.764 kb on + strand, within Psest_3127 at 3304.765 kb on - strand, within Psest_3127 at 3304.765 kb on - strand, within Psest_3127 at 3304.884 kb on + strand at 3304.884 kb on + strand at 3304.884 kb on + strand at 3304.884 kb on + strand at 3304.884 kb on + strand at 3304.884 kb on + strand at 3304.885 kb on - strand at 3304.885 kb on - strand at 3304.885 kb on - strand at 3304.885 kb on - strand at 3304.885 kb on - strand at 3304.885 kb on - strand at 3304.885 kb on - strand at 3304.885 kb on - strand at 3304.917 kb on - strand at 3304.917 kb on - strand at 3305.165 kb on - strand, within Psest_3128 at 3305.165 kb on - strand, within Psest_3128 at 3305.165 kb on - strand, within Psest_3128 at 3305.218 kb on + strand, within Psest_3128 at 3305.218 kb on + strand, within Psest_3128 at 3305.218 kb on + strand, within Psest_3128 at 3305.219 kb on - strand, within Psest_3128 at 3305.219 kb on - strand, within Psest_3128 at 3305.219 kb on - strand, within Psest_3128 at 3305.219 kb on - strand, within Psest_3128 at 3305.223 kb on - strand, within Psest_3128
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.012 mg/ml remove 3,302,943 - +1.1 3,302,943 - -1.0 3,302,943 - +0.7 3,304,326 - +1.1 3,304,486 + Psest_3127 0.26 +2.4 3,304,487 - Psest_3127 0.26 -0.6 3,304,487 - Psest_3127 0.26 +1.5 3,304,487 - Psest_3127 0.26 +0.8 3,304,487 - Psest_3127 0.26 +1.5 3,304,487 - Psest_3127 0.26 +0.8 3,304,487 - Psest_3127 0.26 -0.7 3,304,555 + Psest_3127 0.40 +2.0 3,304,556 - Psest_3127 0.40 -0.0 3,304,556 - Psest_3127 0.40 +0.3 3,304,660 - Psest_3127 0.62 -0.2 3,304,748 - Psest_3127 0.80 +1.0 3,304,764 + Psest_3127 0.83 +2.7 3,304,764 + Psest_3127 0.83 +1.0 3,304,765 - Psest_3127 0.83 -0.7 3,304,765 - Psest_3127 0.83 +0.3 3,304,884 + -0.6 3,304,884 + -2.8 3,304,884 + -2.2 3,304,884 + -1.4 3,304,884 + -0.4 3,304,884 + +0.1 3,304,885 - +0.6 3,304,885 - -2.7 3,304,885 - +0.4 3,304,885 - +1.6 3,304,885 - +1.1 3,304,885 - +1.5 3,304,885 - +0.4 3,304,885 - +0.2 3,304,917 - -1.1 3,304,917 - +0.5 3,305,165 - Psest_3128 0.30 -1.7 3,305,165 - Psest_3128 0.30 -0.4 3,305,165 - Psest_3128 0.30 -2.5 3,305,218 + Psest_3128 0.37 -1.1 3,305,218 + Psest_3128 0.37 -0.3 3,305,218 + Psest_3128 0.37 +1.5 3,305,219 - Psest_3128 0.37 -1.1 3,305,219 - Psest_3128 0.37 +1.7 3,305,219 - Psest_3128 0.37 -0.5 3,305,219 - Psest_3128 0.37 -0.7 3,305,223 - Psest_3128 0.38 -1.7
Or see this region's nucleotide sequence