Experiment: Phosphomycin 0.012 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0028 and Psest_0029 are separated by 43 nucleotides Psest_0029 and Psest_0030 overlap by 4 nucleotides Psest_0030 and Psest_0031 are separated by 12 nucleotides
Psest_0028: Psest_0028 - Coproporphyrinogen III oxidase, at 28,783 to 29,700
_0028
Psest_0029: Psest_0029 - shikimate 5-dehydrogenase, at 29,744 to 30,556
_0029
Psest_0030: Psest_0030 - Putative NADH-flavin reductase, at 30,553 to 31,164
_0030
Psest_0031: Psest_0031 - Uncharacterized conserved protein, at 31,177 to 31,485
_0031
Position (kb) 29
30
31 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 28.750 kb on - strand at 28.813 kb on + strand at 28.813 kb on + strand at 28.813 kb on + strand at 29.086 kb on + strand, within Psest_0028 at 29.087 kb on - strand, within Psest_0028 at 29.186 kb on - strand, within Psest_0028 at 29.410 kb on + strand, within Psest_0028 at 29.410 kb on + strand, within Psest_0028 at 29.410 kb on + strand, within Psest_0028 at 29.503 kb on + strand, within Psest_0028 at 29.512 kb on + strand, within Psest_0028 at 29.512 kb on + strand, within Psest_0028 at 29.512 kb on + strand, within Psest_0028 at 29.513 kb on - strand, within Psest_0028 at 29.513 kb on - strand, within Psest_0028 at 29.513 kb on - strand, within Psest_0028 at 29.513 kb on - strand, within Psest_0028 at 29.513 kb on - strand, within Psest_0028 at 29.513 kb on - strand, within Psest_0028 at 29.524 kb on + strand, within Psest_0028 at 29.611 kb on + strand at 29.660 kb on - strand at 29.660 kb on - strand at 30.550 kb on + strand at 30.550 kb on + strand at 30.551 kb on - strand at 30.551 kb on - strand at 30.554 kb on + strand at 30.625 kb on - strand, within Psest_0030 at 30.869 kb on + strand, within Psest_0030 at 30.870 kb on - strand, within Psest_0030 at 30.974 kb on - strand, within Psest_0030 at 31.125 kb on - strand at 31.125 kb on - strand at 31.310 kb on + strand, within Psest_0031 at 31.311 kb on - strand, within Psest_0031
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.012 mg/ml remove 28,750 - +0.5 28,813 + +0.6 28,813 + -0.3 28,813 + +0.4 29,086 + Psest_0028 0.33 +2.2 29,087 - Psest_0028 0.33 -1.5 29,186 - Psest_0028 0.44 -1.0 29,410 + Psest_0028 0.68 +0.9 29,410 + Psest_0028 0.68 -1.4 29,410 + Psest_0028 0.68 +1.0 29,503 + Psest_0028 0.78 -0.7 29,512 + Psest_0028 0.79 +0.8 29,512 + Psest_0028 0.79 -2.3 29,512 + Psest_0028 0.79 -0.7 29,513 - Psest_0028 0.80 +0.6 29,513 - Psest_0028 0.80 -0.7 29,513 - Psest_0028 0.80 +2.5 29,513 - Psest_0028 0.80 +0.9 29,513 - Psest_0028 0.80 +0.5 29,513 - Psest_0028 0.80 -1.8 29,524 + Psest_0028 0.81 -1.4 29,611 + -0.4 29,660 - +0.3 29,660 - -0.7 30,550 + +0.0 30,550 + +0.2 30,551 - +1.2 30,551 - +0.4 30,554 + -3.3 30,625 - Psest_0030 0.12 +0.1 30,869 + Psest_0030 0.52 +0.4 30,870 - Psest_0030 0.52 -0.0 30,974 - Psest_0030 0.69 +2.1 31,125 - -0.6 31,125 - -0.1 31,310 + Psest_0031 0.43 -2.2 31,311 - Psest_0031 0.43 +1.4
Or see this region's nucleotide sequence