Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0218

Experiment: Chloride 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0217 and Psest_0218 are separated by 9 nucleotidesPsest_0218 and Psest_0219 are separated by 223 nucleotides Psest_0217: Psest_0217 - diguanylate cyclase (GGDEF) domain, at 223,741 to 224,955 _0217 Psest_0218: Psest_0218 - Acyl-CoA hydrolase, at 224,965 to 225,366 _0218 Psest_0219: Psest_0219 - phosphate binding protein, at 225,590 to 226,558 _0219 Position (kb) 224 225 226Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 224.050 kb on + strand, within Psest_0217at 224.050 kb on + strand, within Psest_0217at 224.050 kb on + strand, within Psest_0217at 224.051 kb on - strand, within Psest_0217at 224.051 kb on - strand, within Psest_0217at 224.094 kb on - strand, within Psest_0217at 224.150 kb on + strand, within Psest_0217at 224.151 kb on - strand, within Psest_0217at 224.332 kb on + strand, within Psest_0217at 224.332 kb on + strand, within Psest_0217at 224.332 kb on + strand, within Psest_0217at 224.332 kb on + strand, within Psest_0217at 224.332 kb on + strand, within Psest_0217at 224.332 kb on + strand, within Psest_0217at 224.332 kb on + strand, within Psest_0217at 224.332 kb on + strand, within Psest_0217at 224.333 kb on - strand, within Psest_0217at 224.333 kb on - strand, within Psest_0217at 224.333 kb on - strand, within Psest_0217at 224.333 kb on - strand, within Psest_0217at 224.333 kb on - strand, within Psest_0217at 224.335 kb on + strand, within Psest_0217at 224.335 kb on + strand, within Psest_0217at 224.335 kb on + strand, within Psest_0217at 224.335 kb on + strand, within Psest_0217at 224.335 kb on + strand, within Psest_0217at 224.335 kb on + strand, within Psest_0217at 224.335 kb on + strand, within Psest_0217at 224.336 kb on - strand, within Psest_0217at 224.336 kb on - strand, within Psest_0217at 224.372 kb on - strand, within Psest_0217at 224.372 kb on - strand, within Psest_0217at 224.384 kb on - strand, within Psest_0217at 224.566 kb on + strand, within Psest_0217at 224.566 kb on + strand, within Psest_0217at 224.566 kb on + strand, within Psest_0217at 224.566 kb on + strand, within Psest_0217at 224.566 kb on + strand, within Psest_0217at 224.566 kb on + strand, within Psest_0217at 224.567 kb on - strand, within Psest_0217at 224.567 kb on - strand, within Psest_0217at 224.567 kb on - strand, within Psest_0217at 224.567 kb on - strand, within Psest_0217at 224.567 kb on - strand, within Psest_0217at 224.567 kb on - strand, within Psest_0217at 224.567 kb on - strand, within Psest_0217at 224.698 kb on + strand, within Psest_0217at 224.698 kb on + strand, within Psest_0217at 224.698 kb on + strand, within Psest_0217at 224.698 kb on + strand, within Psest_0217at 224.698 kb on + strand, within Psest_0217at 224.699 kb on - strand, within Psest_0217at 224.699 kb on - strand, within Psest_0217at 224.699 kb on - strand, within Psest_0217at 224.699 kb on - strand, within Psest_0217at 224.762 kb on + strand, within Psest_0217at 224.762 kb on + strand, within Psest_0217at 224.763 kb on - strand, within Psest_0217at 224.763 kb on - strand, within Psest_0217at 224.766 kb on + strand, within Psest_0217at 224.766 kb on + strand, within Psest_0217at 224.766 kb on + strand, within Psest_0217at 224.767 kb on - strand, within Psest_0217at 224.854 kb on + strandat 224.879 kb on - strandat 225.128 kb on + strand, within Psest_0218at 225.129 kb on - strand, within Psest_0218at 225.129 kb on - strand, within Psest_0218at 225.129 kb on - strand, within Psest_0218at 225.129 kb on - strand, within Psest_0218at 225.129 kb on - strand, within Psest_0218at 225.129 kb on - strand, within Psest_0218at 225.269 kb on + strand, within Psest_0218at 225.269 kb on + strand, within Psest_0218at 225.417 kb on - strandat 225.417 kb on - strandat 225.438 kb on + strandat 225.439 kb on - strandat 225.439 kb on - strandat 225.516 kb on + strandat 225.516 kb on + strandat 225.516 kb on + strandat 225.516 kb on + strandat 225.516 kb on + strandat 225.516 kb on + strandat 225.517 kb on - strandat 225.517 kb on - strandat 225.517 kb on - strandat 225.517 kb on - strandat 225.528 kb on - strandat 225.540 kb on + strandat 225.578 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Chloride 200 mM
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224,050 + Psest_0217 0.25 +1.2
224,050 + Psest_0217 0.25 -0.7
224,050 + Psest_0217 0.25 -0.9
224,051 - Psest_0217 0.26 -0.6
224,051 - Psest_0217 0.26 -1.7
224,094 - Psest_0217 0.29 +0.4
224,150 + Psest_0217 0.34 -1.1
224,151 - Psest_0217 0.34 -0.6
224,332 + Psest_0217 0.49 +1.3
224,332 + Psest_0217 0.49 -0.0
224,332 + Psest_0217 0.49 -0.4
224,332 + Psest_0217 0.49 +0.1
224,332 + Psest_0217 0.49 -1.6
224,332 + Psest_0217 0.49 +1.4
224,332 + Psest_0217 0.49 +0.3
224,332 + Psest_0217 0.49 -0.8
224,333 - Psest_0217 0.49 -0.0
224,333 - Psest_0217 0.49 +0.7
224,333 - Psest_0217 0.49 +0.9
224,333 - Psest_0217 0.49 -0.0
224,333 - Psest_0217 0.49 -0.4
224,335 + Psest_0217 0.49 -2.9
224,335 + Psest_0217 0.49 +0.6
224,335 + Psest_0217 0.49 -2.0
224,335 + Psest_0217 0.49 +0.1
224,335 + Psest_0217 0.49 -1.0
224,335 + Psest_0217 0.49 +0.8
224,335 + Psest_0217 0.49 +2.2
224,336 - Psest_0217 0.49 -0.4
224,336 - Psest_0217 0.49 -0.5
224,372 - Psest_0217 0.52 +1.1
224,372 - Psest_0217 0.52 -0.2
224,384 - Psest_0217 0.53 +0.0
224,566 + Psest_0217 0.68 +1.1
224,566 + Psest_0217 0.68 +0.3
224,566 + Psest_0217 0.68 -0.7
224,566 + Psest_0217 0.68 -0.1
224,566 + Psest_0217 0.68 -0.2
224,566 + Psest_0217 0.68 -0.5
224,567 - Psest_0217 0.68 +0.8
224,567 - Psest_0217 0.68 +0.3
224,567 - Psest_0217 0.68 +0.9
224,567 - Psest_0217 0.68 -0.9
224,567 - Psest_0217 0.68 +1.8
224,567 - Psest_0217 0.68 -1.2
224,567 - Psest_0217 0.68 -0.7
224,698 + Psest_0217 0.79 +2.8
224,698 + Psest_0217 0.79 +2.0
224,698 + Psest_0217 0.79 +0.6
224,698 + Psest_0217 0.79 +0.0
224,698 + Psest_0217 0.79 +0.1
224,699 - Psest_0217 0.79 +1.6
224,699 - Psest_0217 0.79 +0.7
224,699 - Psest_0217 0.79 -0.3
224,699 - Psest_0217 0.79 +1.9
224,762 + Psest_0217 0.84 -0.7
224,762 + Psest_0217 0.84 +0.3
224,763 - Psest_0217 0.84 +1.1
224,763 - Psest_0217 0.84 +0.0
224,766 + Psest_0217 0.84 -0.3
224,766 + Psest_0217 0.84 +2.1
224,766 + Psest_0217 0.84 +1.0
224,767 - Psest_0217 0.84 +0.6
224,854 + -0.7
224,879 - -0.8
225,128 + Psest_0218 0.41 -1.3
225,129 - Psest_0218 0.41 -1.3
225,129 - Psest_0218 0.41 -2.1
225,129 - Psest_0218 0.41 -2.5
225,129 - Psest_0218 0.41 -3.6
225,129 - Psest_0218 0.41 -4.0
225,129 - Psest_0218 0.41 +0.6
225,269 + Psest_0218 0.76 -3.3
225,269 + Psest_0218 0.76 -1.9
225,417 - -0.5
225,417 - -0.1
225,438 + -1.1
225,439 - -0.1
225,439 - +0.0
225,516 + -1.6
225,516 + -0.5
225,516 + -0.7
225,516 + -0.5
225,516 + +0.7
225,516 + -0.1
225,517 - -0.1
225,517 - -0.8
225,517 - +0.4
225,517 - -1.0
225,528 - -1.7
225,540 + -0.3
225,578 + -1.4

Or see this region's nucleotide sequence