Experiment: Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3548 and Psest_3549 overlap by 4 nucleotides Psest_3549 and Psest_3550 are separated by 59 nucleotides Psest_3550 and Psest_3551 are separated by 11 nucleotides
Psest_3548: Psest_3548 - transcription antitermination factor NusB, at 3,727,746 to 3,728,231
_3548
Psest_3549: Psest_3549 - 6,7-dimethyl-8-ribityllumazine synthase, at 3,728,228 to 3,728,704
_3549
Psest_3550: Psest_3550 - 3,4-dihydroxy-2-butanone 4-phosphate synthase, at 3,728,764 to 3,729,843
_3550
Psest_3551: Psest_3551 - riboflavin synthase, alpha subunit, at 3,729,855 to 3,730,517
_3551
Position (kb)
3728
3729
3730 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3728.781 kb on - strand at 3728.781 kb on - strand at 3728.786 kb on - strand at 3728.786 kb on - strand at 3728.786 kb on - strand at 3728.786 kb on - strand at 3729.044 kb on - strand, within Psest_3550 at 3729.172 kb on - strand, within Psest_3550 at 3729.172 kb on - strand, within Psest_3550 at 3729.172 kb on - strand, within Psest_3550 at 3730.587 kb on - strand at 3730.587 kb on - strand at 3730.661 kb on - strand at 3730.661 kb on - strand at 3730.661 kb on - strand at 3730.661 kb on - strand at 3730.661 kb on - strand at 3730.724 kb on - strand at 3730.812 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 200 mM remove 3,728,781 - -1.8 3,728,781 - +0.5 3,728,786 - -0.5 3,728,786 - -0.9 3,728,786 - -0.9 3,728,786 - -0.4 3,729,044 - Psest_3550 0.26 +0.2 3,729,172 - Psest_3550 0.38 +1.0 3,729,172 - Psest_3550 0.38 +0.6 3,729,172 - Psest_3550 0.38 +4.1 3,730,587 - +1.4 3,730,587 - -0.4 3,730,661 - -1.8 3,730,661 - +0.4 3,730,661 - -0.2 3,730,661 - -3.1 3,730,661 - +1.6 3,730,724 - +0.8 3,730,812 + +0.2
Or see this region's nucleotide sequence