Experiment: Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2014 and Psest_2015 are separated by 484 nucleotides Psest_2015 and Psest_2016 overlap by 4 nucleotides Psest_2016 and Psest_2017 are separated by 6 nucleotides
Psest_2014: Psest_2014 - Isocitrate lyase (EC 4.1.3.1) (from data) , at 2,132,881 to 2,134,476
_2014
Psest_2015: Psest_2015 - Type II secretory pathway, component PulD, at 2,134,961 to 2,135,770
_2015
Psest_2016: Psest_2016 - hypothetical protein, at 2,135,767 to 2,136,396
_2016
Psest_2017: Psest_2017 - Predicted acyltransferase, at 2,136,403 to 2,136,828
_2017
Position (kb)
2134
2135
2136 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2134.076 kb on + strand, within Psest_2014 at 2134.076 kb on + strand, within Psest_2014 at 2134.076 kb on + strand, within Psest_2014 at 2134.077 kb on - strand, within Psest_2014 at 2134.077 kb on - strand, within Psest_2014 at 2134.181 kb on + strand, within Psest_2014 at 2134.334 kb on + strand at 2134.424 kb on + strand at 2134.466 kb on + strand at 2134.493 kb on + strand at 2134.493 kb on + strand at 2134.493 kb on + strand at 2134.493 kb on + strand at 2134.530 kb on + strand at 2134.531 kb on - strand at 2134.580 kb on - strand at 2134.783 kb on - strand at 2134.923 kb on + strand at 2135.217 kb on + strand, within Psest_2015 at 2135.217 kb on + strand, within Psest_2015 at 2135.217 kb on + strand, within Psest_2015 at 2135.217 kb on + strand, within Psest_2015 at 2135.218 kb on - strand, within Psest_2015 at 2135.218 kb on - strand, within Psest_2015 at 2135.396 kb on + strand, within Psest_2015 at 2135.397 kb on - strand, within Psest_2015 at 2135.616 kb on + strand, within Psest_2015 at 2135.646 kb on - strand, within Psest_2015 at 2135.717 kb on + strand at 2135.717 kb on + strand at 2135.718 kb on - strand at 2135.758 kb on + strand at 2135.841 kb on + strand, within Psest_2016 at 2135.842 kb on - strand, within Psest_2016 at 2135.864 kb on + strand, within Psest_2016 at 2135.865 kb on - strand, within Psest_2016 at 2135.865 kb on - strand, within Psest_2016 at 2135.865 kb on - strand, within Psest_2016 at 2135.865 kb on - strand, within Psest_2016 at 2135.865 kb on - strand, within Psest_2016 at 2135.873 kb on + strand, within Psest_2016 at 2135.908 kb on + strand, within Psest_2016 at 2135.934 kb on - strand, within Psest_2016 at 2135.976 kb on + strand, within Psest_2016 at 2136.005 kb on - strand, within Psest_2016 at 2136.005 kb on - strand, within Psest_2016 at 2136.128 kb on + strand, within Psest_2016 at 2136.128 kb on + strand, within Psest_2016 at 2136.489 kb on - strand, within Psest_2017 at 2136.489 kb on - strand, within Psest_2017 at 2136.489 kb on - strand, within Psest_2017 at 2136.533 kb on - strand, within Psest_2017
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 200 mM remove 2,134,076 + Psest_2014 0.75 +0.3 2,134,076 + Psest_2014 0.75 +0.2 2,134,076 + Psest_2014 0.75 -0.6 2,134,077 - Psest_2014 0.75 -0.8 2,134,077 - Psest_2014 0.75 -0.1 2,134,181 + Psest_2014 0.81 -0.5 2,134,334 + +1.5 2,134,424 + +0.3 2,134,466 + -2.3 2,134,493 + -0.7 2,134,493 + -0.3 2,134,493 + +0.4 2,134,493 + +0.3 2,134,530 + -1.8 2,134,531 - +0.6 2,134,580 - +0.2 2,134,783 - +0.9 2,134,923 + -0.3 2,135,217 + Psest_2015 0.32 +1.2 2,135,217 + Psest_2015 0.32 -0.2 2,135,217 + Psest_2015 0.32 +0.3 2,135,217 + Psest_2015 0.32 +2.2 2,135,218 - Psest_2015 0.32 -0.0 2,135,218 - Psest_2015 0.32 -1.3 2,135,396 + Psest_2015 0.54 +0.7 2,135,397 - Psest_2015 0.54 -0.6 2,135,616 + Psest_2015 0.81 +1.5 2,135,646 - Psest_2015 0.85 +0.3 2,135,717 + +0.6 2,135,717 + -0.2 2,135,718 - +0.3 2,135,758 + +0.2 2,135,841 + Psest_2016 0.12 -1.0 2,135,842 - Psest_2016 0.12 -1.4 2,135,864 + Psest_2016 0.15 -0.9 2,135,865 - Psest_2016 0.16 -3.8 2,135,865 - Psest_2016 0.16 +0.9 2,135,865 - Psest_2016 0.16 -0.2 2,135,865 - Psest_2016 0.16 +0.3 2,135,865 - Psest_2016 0.16 -1.3 2,135,873 + Psest_2016 0.17 -0.8 2,135,908 + Psest_2016 0.22 +0.6 2,135,934 - Psest_2016 0.27 +1.1 2,135,976 + Psest_2016 0.33 -0.5 2,136,005 - Psest_2016 0.38 -0.8 2,136,005 - Psest_2016 0.38 -1.0 2,136,128 + Psest_2016 0.57 +0.8 2,136,128 + Psest_2016 0.57 -0.3 2,136,489 - Psest_2017 0.20 -1.3 2,136,489 - Psest_2017 0.20 -0.6 2,136,489 - Psest_2017 0.20 -0.1 2,136,533 - Psest_2017 0.31 -0.6
Or see this region's nucleotide sequence