Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_2015

Experiment: Chloride 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_2014 and Psest_2015 are separated by 484 nucleotidesPsest_2015 and Psest_2016 overlap by 4 nucleotidesPsest_2016 and Psest_2017 are separated by 6 nucleotides Psest_2014: Psest_2014 - Isocitrate lyase (EC 4.1.3.1) (from data), at 2,132,881 to 2,134,476 _2014 Psest_2015: Psest_2015 - Type II secretory pathway, component PulD, at 2,134,961 to 2,135,770 _2015 Psest_2016: Psest_2016 - hypothetical protein, at 2,135,767 to 2,136,396 _2016 Psest_2017: Psest_2017 - Predicted acyltransferase, at 2,136,403 to 2,136,828 _2017 Position (kb) 2134 2135 2136Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2134.076 kb on + strand, within Psest_2014at 2134.076 kb on + strand, within Psest_2014at 2134.076 kb on + strand, within Psest_2014at 2134.077 kb on - strand, within Psest_2014at 2134.077 kb on - strand, within Psest_2014at 2134.181 kb on + strand, within Psest_2014at 2134.334 kb on + strandat 2134.424 kb on + strandat 2134.466 kb on + strandat 2134.493 kb on + strandat 2134.493 kb on + strandat 2134.493 kb on + strandat 2134.493 kb on + strandat 2134.530 kb on + strandat 2134.531 kb on - strandat 2134.580 kb on - strandat 2134.783 kb on - strandat 2134.923 kb on + strandat 2135.217 kb on + strand, within Psest_2015at 2135.217 kb on + strand, within Psest_2015at 2135.217 kb on + strand, within Psest_2015at 2135.217 kb on + strand, within Psest_2015at 2135.218 kb on - strand, within Psest_2015at 2135.218 kb on - strand, within Psest_2015at 2135.396 kb on + strand, within Psest_2015at 2135.397 kb on - strand, within Psest_2015at 2135.616 kb on + strand, within Psest_2015at 2135.646 kb on - strand, within Psest_2015at 2135.717 kb on + strandat 2135.717 kb on + strandat 2135.718 kb on - strandat 2135.758 kb on + strandat 2135.841 kb on + strand, within Psest_2016at 2135.842 kb on - strand, within Psest_2016at 2135.864 kb on + strand, within Psest_2016at 2135.865 kb on - strand, within Psest_2016at 2135.865 kb on - strand, within Psest_2016at 2135.865 kb on - strand, within Psest_2016at 2135.865 kb on - strand, within Psest_2016at 2135.865 kb on - strand, within Psest_2016at 2135.873 kb on + strand, within Psest_2016at 2135.908 kb on + strand, within Psest_2016at 2135.934 kb on - strand, within Psest_2016at 2135.976 kb on + strand, within Psest_2016at 2136.005 kb on - strand, within Psest_2016at 2136.005 kb on - strand, within Psest_2016at 2136.128 kb on + strand, within Psest_2016at 2136.128 kb on + strand, within Psest_2016at 2136.489 kb on - strand, within Psest_2017at 2136.489 kb on - strand, within Psest_2017at 2136.489 kb on - strand, within Psest_2017at 2136.533 kb on - strand, within Psest_2017

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Per-strain Table

Position Strand Gene LocusTag Fraction Chloride 200 mM
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2,134,076 + Psest_2014 0.75 +0.3
2,134,076 + Psest_2014 0.75 +0.2
2,134,076 + Psest_2014 0.75 -0.6
2,134,077 - Psest_2014 0.75 -0.8
2,134,077 - Psest_2014 0.75 -0.1
2,134,181 + Psest_2014 0.81 -0.5
2,134,334 + +1.5
2,134,424 + +0.3
2,134,466 + -2.3
2,134,493 + -0.7
2,134,493 + -0.3
2,134,493 + +0.4
2,134,493 + +0.3
2,134,530 + -1.8
2,134,531 - +0.6
2,134,580 - +0.2
2,134,783 - +0.9
2,134,923 + -0.3
2,135,217 + Psest_2015 0.32 +1.2
2,135,217 + Psest_2015 0.32 -0.2
2,135,217 + Psest_2015 0.32 +0.3
2,135,217 + Psest_2015 0.32 +2.2
2,135,218 - Psest_2015 0.32 -0.0
2,135,218 - Psest_2015 0.32 -1.3
2,135,396 + Psest_2015 0.54 +0.7
2,135,397 - Psest_2015 0.54 -0.6
2,135,616 + Psest_2015 0.81 +1.5
2,135,646 - Psest_2015 0.85 +0.3
2,135,717 + +0.6
2,135,717 + -0.2
2,135,718 - +0.3
2,135,758 + +0.2
2,135,841 + Psest_2016 0.12 -1.0
2,135,842 - Psest_2016 0.12 -1.4
2,135,864 + Psest_2016 0.15 -0.9
2,135,865 - Psest_2016 0.16 -3.8
2,135,865 - Psest_2016 0.16 +0.9
2,135,865 - Psest_2016 0.16 -0.2
2,135,865 - Psest_2016 0.16 +0.3
2,135,865 - Psest_2016 0.16 -1.3
2,135,873 + Psest_2016 0.17 -0.8
2,135,908 + Psest_2016 0.22 +0.6
2,135,934 - Psest_2016 0.27 +1.1
2,135,976 + Psest_2016 0.33 -0.5
2,136,005 - Psest_2016 0.38 -0.8
2,136,005 - Psest_2016 0.38 -1.0
2,136,128 + Psest_2016 0.57 +0.8
2,136,128 + Psest_2016 0.57 -0.3
2,136,489 - Psest_2017 0.20 -1.3
2,136,489 - Psest_2017 0.20 -0.6
2,136,489 - Psest_2017 0.20 -0.1
2,136,533 - Psest_2017 0.31 -0.6

Or see this region's nucleotide sequence