Experiment: Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1988 and Psest_1989 are separated by 93 nucleotides Psest_1989 and Psest_1990 are separated by 74 nucleotides Psest_1990 and Psest_1991 are separated by 46 nucleotides Psest_1991 and Psest_1992 are separated by 46 nucleotides
Psest_1988: Psest_1988 - ribosomal protein S1, at 2,105,689 to 2,107,371
_1988
Psest_1989: Psest_1989 - Transcriptional regulators, at 2,107,465 to 2,107,908
_1989
Psest_1990: Psest_1990 - Transcriptional regulators containing a DNA-binding HTH domain and an aminotransferase domain (MocR family) and their eukaryotic orthologs, at 2,107,983 to 2,109,155
_1990
Psest_1991: Psest_1991 - Glutathione S-transferase, at 2,109,202 to 2,109,804
_1991
Psest_1992: Psest_1992 - GTP cyclohydrolase I, at 2,109,851 to 2,110,396
_1992
Position (kb)
2107
2108
2109
2110 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2107.555 kb on + strand, within Psest_1989 at 2107.624 kb on - strand, within Psest_1989 at 2107.624 kb on - strand, within Psest_1989 at 2107.624 kb on - strand, within Psest_1989 at 2107.624 kb on - strand, within Psest_1989 at 2107.629 kb on + strand, within Psest_1989 at 2107.630 kb on - strand, within Psest_1989 at 2107.650 kb on + strand, within Psest_1989 at 2107.651 kb on - strand, within Psest_1989 at 2107.724 kb on + strand, within Psest_1989 at 2107.724 kb on + strand, within Psest_1989 at 2107.728 kb on + strand, within Psest_1989 at 2107.728 kb on + strand, within Psest_1989 at 2107.729 kb on - strand, within Psest_1989 at 2107.729 kb on - strand, within Psest_1989 at 2107.871 kb on - strand at 2107.958 kb on + strand at 2108.152 kb on - strand, within Psest_1990 at 2108.331 kb on + strand, within Psest_1990 at 2108.584 kb on + strand, within Psest_1990 at 2108.643 kb on + strand, within Psest_1990 at 2108.644 kb on - strand, within Psest_1990 at 2109.215 kb on - strand at 2109.215 kb on - strand at 2109.223 kb on + strand at 2109.241 kb on + strand at 2109.241 kb on + strand at 2109.241 kb on + strand at 2109.242 kb on - strand at 2109.280 kb on + strand, within Psest_1991 at 2109.281 kb on - strand, within Psest_1991 at 2109.418 kb on + strand, within Psest_1991 at 2109.418 kb on + strand, within Psest_1991 at 2109.419 kb on - strand, within Psest_1991 at 2109.419 kb on - strand, within Psest_1991 at 2109.419 kb on - strand, within Psest_1991 at 2109.544 kb on + strand, within Psest_1991 at 2109.552 kb on + strand, within Psest_1991 at 2109.577 kb on + strand, within Psest_1991 at 2109.577 kb on + strand, within Psest_1991 at 2109.646 kb on + strand, within Psest_1991 at 2109.646 kb on + strand, within Psest_1991 at 2109.647 kb on - strand, within Psest_1991 at 2109.664 kb on + strand, within Psest_1991 at 2109.665 kb on - strand, within Psest_1991 at 2109.686 kb on + strand, within Psest_1991 at 2109.686 kb on + strand, within Psest_1991 at 2109.686 kb on + strand, within Psest_1991 at 2109.686 kb on + strand, within Psest_1991 at 2109.687 kb on - strand, within Psest_1991 at 2109.687 kb on - strand, within Psest_1991 at 2109.688 kb on + strand, within Psest_1991 at 2109.688 kb on + strand, within Psest_1991 at 2109.688 kb on + strand, within Psest_1991 at 2109.688 kb on + strand, within Psest_1991 at 2109.688 kb on + strand, within Psest_1991 at 2109.688 kb on + strand, within Psest_1991 at 2109.688 kb on + strand, within Psest_1991 at 2109.688 kb on + strand, within Psest_1991 at 2109.689 kb on - strand, within Psest_1991 at 2109.689 kb on - strand, within Psest_1991 at 2109.689 kb on - strand, within Psest_1991 at 2109.694 kb on + strand, within Psest_1991 at 2109.694 kb on + strand, within Psest_1991 at 2109.694 kb on + strand, within Psest_1991 at 2109.695 kb on - strand, within Psest_1991 at 2109.695 kb on - strand, within Psest_1991 at 2109.695 kb on - strand, within Psest_1991 at 2109.793 kb on - strand at 2109.796 kb on + strand at 2109.797 kb on - strand at 2109.797 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 200 mM remove 2,107,555 + Psest_1989 0.20 +1.3 2,107,624 - Psest_1989 0.36 +1.0 2,107,624 - Psest_1989 0.36 +0.2 2,107,624 - Psest_1989 0.36 -0.9 2,107,624 - Psest_1989 0.36 -2.3 2,107,629 + Psest_1989 0.37 +1.1 2,107,630 - Psest_1989 0.37 -0.4 2,107,650 + Psest_1989 0.42 -0.9 2,107,651 - Psest_1989 0.42 -0.9 2,107,724 + Psest_1989 0.58 -1.5 2,107,724 + Psest_1989 0.58 -0.7 2,107,728 + Psest_1989 0.59 -0.6 2,107,728 + Psest_1989 0.59 -0.3 2,107,729 - Psest_1989 0.59 -0.6 2,107,729 - Psest_1989 0.59 +0.6 2,107,871 - +1.8 2,107,958 + +0.1 2,108,152 - Psest_1990 0.14 -1.0 2,108,331 + Psest_1990 0.30 +1.9 2,108,584 + Psest_1990 0.51 +2.3 2,108,643 + Psest_1990 0.56 +0.3 2,108,644 - Psest_1990 0.56 +2.4 2,109,215 - -1.3 2,109,215 - -1.2 2,109,223 + -0.9 2,109,241 + +1.0 2,109,241 + -1.1 2,109,241 + -2.3 2,109,242 - -2.4 2,109,280 + Psest_1991 0.13 +1.4 2,109,281 - Psest_1991 0.13 +1.2 2,109,418 + Psest_1991 0.36 -0.2 2,109,418 + Psest_1991 0.36 -2.0 2,109,419 - Psest_1991 0.36 -0.0 2,109,419 - Psest_1991 0.36 -0.5 2,109,419 - Psest_1991 0.36 -1.9 2,109,544 + Psest_1991 0.57 -1.4 2,109,552 + Psest_1991 0.58 -0.3 2,109,577 + Psest_1991 0.62 +0.7 2,109,577 + Psest_1991 0.62 -0.9 2,109,646 + Psest_1991 0.74 -0.5 2,109,646 + Psest_1991 0.74 +0.2 2,109,647 - Psest_1991 0.74 +0.7 2,109,664 + Psest_1991 0.77 +1.0 2,109,665 - Psest_1991 0.77 -2.6 2,109,686 + Psest_1991 0.80 +1.0 2,109,686 + Psest_1991 0.80 +0.3 2,109,686 + Psest_1991 0.80 -0.2 2,109,686 + Psest_1991 0.80 +1.2 2,109,687 - Psest_1991 0.80 +0.7 2,109,687 - Psest_1991 0.80 -0.7 2,109,688 + Psest_1991 0.81 +2.2 2,109,688 + Psest_1991 0.81 +0.7 2,109,688 + Psest_1991 0.81 -0.4 2,109,688 + Psest_1991 0.81 +1.1 2,109,688 + Psest_1991 0.81 -1.3 2,109,688 + Psest_1991 0.81 +0.3 2,109,688 + Psest_1991 0.81 -1.1 2,109,688 + Psest_1991 0.81 +0.2 2,109,689 - Psest_1991 0.81 -0.7 2,109,689 - Psest_1991 0.81 +0.4 2,109,689 - Psest_1991 0.81 +0.8 2,109,694 + Psest_1991 0.82 +0.4 2,109,694 + Psest_1991 0.82 +0.1 2,109,694 + Psest_1991 0.82 -1.9 2,109,695 - Psest_1991 0.82 -0.8 2,109,695 - Psest_1991 0.82 +3.0 2,109,695 - Psest_1991 0.82 +0.6 2,109,793 - +1.2 2,109,796 + +0.6 2,109,797 - -1.7 2,109,797 - +1.0
Or see this region's nucleotide sequence