Experiment: Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1973 and Psest_1974 are separated by 3 nucleotides Psest_1974 and Psest_1975 overlap by 20 nucleotides Psest_1975 and Psest_1976 are separated by 85 nucleotides Psest_1976 and Psest_1978 are separated by 442 nucleotides
Psest_1973: Psest_1973 - phenylalanyl-tRNA synthetase, beta subunit, non-spirochete bacterial, at 2,085,991 to 2,088,369
_1973
Psest_1974: Psest_1974 - integration host factor, alpha subunit, at 2,088,373 to 2,088,675
_1974
Psest_1975: Psest_1975 - Predicted transcriptional regulators, at 2,088,656 to 2,089,012
_1975
Psest_1976: Psest_1976 - tRNA_Pro_GGG, at 2,089,098 to 2,089,174
_1976
Psest_1978: Psest_1978 - DNA-directed DNA polymerase III (polc), at 2,089,617 to 2,092,697
_1978
Position (kb)
2088
2089 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2088.367 kb on + strand at 2088.367 kb on + strand at 2088.368 kb on - strand at 2088.412 kb on + strand, within Psest_1974 at 2088.413 kb on - strand, within Psest_1974 at 2088.413 kb on - strand, within Psest_1974 at 2088.413 kb on - strand, within Psest_1974 at 2088.433 kb on - strand, within Psest_1974 at 2088.433 kb on - strand, within Psest_1974 at 2088.655 kb on + strand at 2088.655 kb on + strand at 2088.655 kb on + strand at 2088.873 kb on - strand, within Psest_1975 at 2088.910 kb on + strand, within Psest_1975 at 2088.910 kb on + strand, within Psest_1975 at 2088.910 kb on + strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.948 kb on - strand, within Psest_1975 at 2088.958 kb on + strand, within Psest_1975 at 2088.959 kb on - strand, within Psest_1975 at 2089.010 kb on + strand at 2089.010 kb on + strand at 2089.010 kb on + strand at 2089.010 kb on + strand at 2089.054 kb on - strand at 2089.058 kb on + strand at 2089.058 kb on + strand at 2089.058 kb on + strand at 2089.058 kb on + strand at 2089.060 kb on + strand at 2089.178 kb on - strand at 2089.521 kb on + strand at 2089.522 kb on - strand at 2089.536 kb on - strand at 2089.567 kb on + strand at 2089.584 kb on + strand at 2089.585 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 200 mM remove 2,088,367 + +0.3 2,088,367 + +2.5 2,088,368 - +0.3 2,088,412 + Psest_1974 0.13 +2.2 2,088,413 - Psest_1974 0.13 +0.6 2,088,413 - Psest_1974 0.13 +3.0 2,088,413 - Psest_1974 0.13 +2.5 2,088,433 - Psest_1974 0.20 +0.1 2,088,433 - Psest_1974 0.20 +0.5 2,088,655 + +0.4 2,088,655 + +0.1 2,088,655 + +0.3 2,088,873 - Psest_1975 0.61 -0.1 2,088,910 + Psest_1975 0.71 +0.5 2,088,910 + Psest_1975 0.71 +0.3 2,088,910 + Psest_1975 0.71 +0.4 2,088,911 - Psest_1975 0.71 -0.3 2,088,911 - Psest_1975 0.71 -0.3 2,088,911 - Psest_1975 0.71 +1.7 2,088,911 - Psest_1975 0.71 +0.3 2,088,911 - Psest_1975 0.71 -1.0 2,088,911 - Psest_1975 0.71 +0.8 2,088,911 - Psest_1975 0.71 -0.3 2,088,911 - Psest_1975 0.71 -0.4 2,088,911 - Psest_1975 0.71 -0.9 2,088,911 - Psest_1975 0.71 +0.1 2,088,911 - Psest_1975 0.71 +1.6 2,088,911 - Psest_1975 0.71 -0.7 2,088,911 - Psest_1975 0.71 -0.2 2,088,911 - Psest_1975 0.71 -0.1 2,088,948 - Psest_1975 0.82 -0.5 2,088,958 + Psest_1975 0.85 +0.7 2,088,959 - Psest_1975 0.85 -1.3 2,089,010 + -0.2 2,089,010 + +1.5 2,089,010 + +0.9 2,089,010 + -0.6 2,089,054 - -0.9 2,089,058 + +0.7 2,089,058 + -0.5 2,089,058 + +0.2 2,089,058 + -0.2 2,089,060 + +1.9 2,089,178 - -0.9 2,089,521 + +0.3 2,089,522 - -1.7 2,089,536 - +0.5 2,089,567 + -2.2 2,089,584 + +0.8 2,089,585 - -0.6
Or see this region's nucleotide sequence