Experiment: Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1862 and Psest_1863 are separated by 60 nucleotides Psest_1863 and Psest_1864 are separated by 158 nucleotides Psest_1864 and Psest_1865 are separated by 57 nucleotides Psest_1865 and Psest_1866 are separated by 49 nucleotides
Psest_1862: Psest_1862 - VCBS repeat/type 1 secretion C-terminal target domain (VC_A0849 subclass), at 1,966,383 to 1,973,558
_1862
Psest_1863: Psest_1863 - hypothetical protein, at 1,973,619 to 1,973,960
_1863
Psest_1864: Psest_1864 - diguanylate cyclase (GGDEF) domain, at 1,974,119 to 1,976,092
_1864
Psest_1865: Psest_1865 - Predicted periplasmic protein, at 1,976,150 to 1,976,866
_1865
Psest_1866: Psest_1866 - Uncharacterized protein conserved in bacteria, at 1,976,916 to 1,977,335
_1866
Position (kb)
1974
1975
1976
1977 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1973.121 kb on - strand at 1973.124 kb on + strand at 1973.124 kb on + strand at 1973.124 kb on + strand at 1973.125 kb on - strand at 1973.273 kb on + strand at 1973.273 kb on + strand at 1973.274 kb on - strand at 1973.335 kb on + strand at 1973.335 kb on + strand at 1973.335 kb on + strand at 1973.335 kb on + strand at 1973.336 kb on - strand at 1973.336 kb on - strand at 1973.412 kb on - strand at 1973.412 kb on - strand at 1973.487 kb on + strand at 1973.487 kb on + strand at 1973.500 kb on + strand at 1973.625 kb on + strand at 1973.625 kb on + strand at 1973.625 kb on + strand at 1973.625 kb on + strand at 1973.625 kb on + strand at 1973.625 kb on + strand at 1973.625 kb on + strand at 1973.626 kb on - strand at 1973.626 kb on - strand at 1973.626 kb on - strand at 1973.626 kb on - strand at 1973.626 kb on - strand at 1973.626 kb on - strand at 1973.626 kb on - strand at 1973.626 kb on - strand at 1973.626 kb on - strand at 1973.626 kb on - strand at 1973.629 kb on + strand at 1973.629 kb on + strand at 1973.629 kb on + strand at 1973.629 kb on + strand at 1973.629 kb on + strand at 1973.630 kb on - strand at 1973.630 kb on - strand at 1973.755 kb on + strand, within Psest_1863 at 1973.755 kb on + strand, within Psest_1863 at 1973.755 kb on + strand, within Psest_1863 at 1973.756 kb on - strand, within Psest_1863 at 1973.924 kb on + strand, within Psest_1863 at 1973.925 kb on - strand, within Psest_1863 at 1973.958 kb on + strand at 1973.959 kb on - strand at 1973.968 kb on + strand at 1973.968 kb on + strand at 1973.972 kb on + strand at 1974.081 kb on - strand at 1974.176 kb on + strand at 1974.176 kb on + strand at 1974.176 kb on + strand at 1974.176 kb on + strand at 1974.177 kb on - strand at 1974.177 kb on - strand at 1974.177 kb on - strand at 1974.177 kb on - strand at 1974.179 kb on + strand at 1974.179 kb on + strand at 1974.179 kb on + strand at 1974.179 kb on + strand at 1974.180 kb on - strand at 1974.180 kb on - strand at 1974.180 kb on - strand at 1974.180 kb on - strand at 1974.180 kb on - strand at 1974.219 kb on - strand at 1974.288 kb on + strand at 1974.343 kb on - strand, within Psest_1864 at 1974.585 kb on - strand, within Psest_1864 at 1974.663 kb on + strand, within Psest_1864 at 1974.663 kb on + strand, within Psest_1864 at 1974.663 kb on + strand, within Psest_1864 at 1974.664 kb on - strand, within Psest_1864 at 1974.664 kb on - strand, within Psest_1864 at 1974.813 kb on + strand, within Psest_1864 at 1975.079 kb on + strand, within Psest_1864 at 1975.214 kb on + strand, within Psest_1864 at 1975.384 kb on - strand, within Psest_1864 at 1975.562 kb on + strand, within Psest_1864 at 1975.694 kb on + strand, within Psest_1864 at 1975.898 kb on + strand at 1975.898 kb on + strand at 1976.021 kb on + strand at 1976.022 kb on - strand at 1976.092 kb on + strand at 1976.092 kb on + strand at 1976.092 kb on + strand at 1976.092 kb on + strand at 1976.092 kb on + strand at 1976.092 kb on + strand at 1976.092 kb on + strand at 1976.092 kb on + strand at 1976.092 kb on + strand at 1976.092 kb on + strand at 1976.092 kb on + strand at 1976.092 kb on + strand at 1976.093 kb on - strand at 1976.093 kb on - strand at 1976.093 kb on - strand at 1976.093 kb on - strand at 1976.093 kb on - strand at 1976.093 kb on - strand at 1976.327 kb on + strand, within Psest_1865 at 1976.327 kb on + strand, within Psest_1865 at 1976.328 kb on - strand, within Psest_1865 at 1976.379 kb on + strand, within Psest_1865 at 1976.379 kb on + strand, within Psest_1865 at 1976.379 kb on + strand, within Psest_1865 at 1976.379 kb on + strand, within Psest_1865 at 1976.380 kb on - strand, within Psest_1865 at 1976.392 kb on + strand, within Psest_1865 at 1976.393 kb on - strand, within Psest_1865 at 1976.411 kb on + strand, within Psest_1865 at 1976.420 kb on + strand, within Psest_1865 at 1976.420 kb on + strand, within Psest_1865 at 1976.420 kb on + strand, within Psest_1865 at 1976.420 kb on + strand, within Psest_1865 at 1976.420 kb on + strand, within Psest_1865 at 1976.421 kb on - strand, within Psest_1865 at 1976.421 kb on - strand, within Psest_1865 at 1976.424 kb on + strand, within Psest_1865 at 1976.424 kb on + strand, within Psest_1865 at 1976.424 kb on + strand, within Psest_1865 at 1976.424 kb on + strand, within Psest_1865 at 1976.424 kb on + strand, within Psest_1865 at 1976.424 kb on + strand, within Psest_1865 at 1976.424 kb on + strand, within Psest_1865 at 1976.424 kb on + strand, within Psest_1865 at 1976.424 kb on + strand, within Psest_1865 at 1976.424 kb on + strand, within Psest_1865 at 1976.424 kb on + strand, within Psest_1865 at 1976.424 kb on + strand, within Psest_1865 at 1976.424 kb on + strand, within Psest_1865 at 1976.425 kb on - strand, within Psest_1865 at 1976.425 kb on - strand, within Psest_1865 at 1976.425 kb on - strand, within Psest_1865 at 1976.425 kb on - strand, within Psest_1865 at 1976.425 kb on - strand, within Psest_1865 at 1976.425 kb on - strand, within Psest_1865 at 1976.425 kb on - strand, within Psest_1865 at 1976.425 kb on - strand, within Psest_1865 at 1976.425 kb on - strand, within Psest_1865 at 1976.425 kb on - strand, within Psest_1865 at 1976.425 kb on - strand, within Psest_1865 at 1976.475 kb on + strand, within Psest_1865 at 1976.475 kb on + strand, within Psest_1865 at 1976.475 kb on + strand, within Psest_1865 at 1976.475 kb on + strand, within Psest_1865 at 1976.475 kb on + strand, within Psest_1865 at 1976.475 kb on + strand, within Psest_1865 at 1976.475 kb on + strand, within Psest_1865 at 1976.475 kb on + strand, within Psest_1865 at 1976.476 kb on - strand, within Psest_1865 at 1976.476 kb on - strand, within Psest_1865 at 1976.490 kb on + strand, within Psest_1865 at 1976.491 kb on - strand, within Psest_1865 at 1976.491 kb on - strand, within Psest_1865 at 1976.544 kb on + strand, within Psest_1865 at 1976.932 kb on - strand at 1976.932 kb on - strand at 1976.932 kb on - strand at 1976.932 kb on - strand at 1976.932 kb on - strand at 1976.932 kb on - strand at 1976.932 kb on - strand at 1976.968 kb on + strand, within Psest_1866 at 1976.968 kb on + strand, within Psest_1866 at 1976.968 kb on + strand, within Psest_1866 at 1976.968 kb on + strand, within Psest_1866 at 1976.969 kb on - strand, within Psest_1866 at 1976.969 kb on - strand, within Psest_1866 at 1976.969 kb on - strand, within Psest_1866 at 1976.969 kb on - strand, within Psest_1866 at 1976.969 kb on - strand, within Psest_1866 at 1976.969 kb on - strand, within Psest_1866 at 1976.969 kb on - strand, within Psest_1866 at 1977.073 kb on + strand, within Psest_1866 at 1977.073 kb on + strand, within Psest_1866 at 1977.073 kb on + strand, within Psest_1866 at 1977.073 kb on + strand, within Psest_1866 at 1977.073 kb on + strand, within Psest_1866 at 1977.074 kb on - strand, within Psest_1866 at 1977.074 kb on - strand, within Psest_1866
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 200 mM remove 1,973,121 - +0.4 1,973,124 + +0.3 1,973,124 + -1.0 1,973,124 + -0.4 1,973,125 - +0.6 1,973,273 + +1.7 1,973,273 + +0.3 1,973,274 - -0.1 1,973,335 + +0.2 1,973,335 + +0.2 1,973,335 + +0.2 1,973,335 + -0.5 1,973,336 - +1.1 1,973,336 - +1.0 1,973,412 - +1.0 1,973,412 - +0.7 1,973,487 + -1.0 1,973,487 + +1.3 1,973,500 + -0.1 1,973,625 + -0.3 1,973,625 + -1.0 1,973,625 + -0.7 1,973,625 + -1.1 1,973,625 + +1.3 1,973,625 + +0.8 1,973,625 + +0.3 1,973,626 - +1.0 1,973,626 - -2.0 1,973,626 - +0.2 1,973,626 - +0.6 1,973,626 - -0.2 1,973,626 - -2.0 1,973,626 - +1.3 1,973,626 - -1.2 1,973,626 - -1.7 1,973,626 - -1.1 1,973,629 + +0.9 1,973,629 + +0.3 1,973,629 + +2.5 1,973,629 + +0.5 1,973,629 + -0.0 1,973,630 - +1.3 1,973,630 - +0.4 1,973,755 + Psest_1863 0.40 -0.7 1,973,755 + Psest_1863 0.40 -0.3 1,973,755 + Psest_1863 0.40 +0.3 1,973,756 - Psest_1863 0.40 -0.0 1,973,924 + Psest_1863 0.89 +2.1 1,973,925 - Psest_1863 0.89 -0.5 1,973,958 + -0.3 1,973,959 - +1.1 1,973,968 + -0.5 1,973,968 + +0.3 1,973,972 + -1.5 1,974,081 - -1.5 1,974,176 + +0.4 1,974,176 + +0.3 1,974,176 + -0.3 1,974,176 + -0.7 1,974,177 - -1.5 1,974,177 - -0.1 1,974,177 - +1.0 1,974,177 - -0.5 1,974,179 + -0.5 1,974,179 + +0.1 1,974,179 + -1.5 1,974,179 + +1.2 1,974,180 - +0.5 1,974,180 - -0.4 1,974,180 - +0.7 1,974,180 - +0.8 1,974,180 - -1.5 1,974,219 - -0.3 1,974,288 + +0.9 1,974,343 - Psest_1864 0.11 -1.7 1,974,585 - Psest_1864 0.24 -0.4 1,974,663 + Psest_1864 0.28 -0.7 1,974,663 + Psest_1864 0.28 -1.2 1,974,663 + Psest_1864 0.28 -0.6 1,974,664 - Psest_1864 0.28 +0.1 1,974,664 - Psest_1864 0.28 -0.5 1,974,813 + Psest_1864 0.35 -0.5 1,975,079 + Psest_1864 0.49 +0.6 1,975,214 + Psest_1864 0.55 -0.6 1,975,384 - Psest_1864 0.64 -1.3 1,975,562 + Psest_1864 0.73 -1.3 1,975,694 + Psest_1864 0.80 +1.2 1,975,898 + +0.0 1,975,898 + -1.0 1,976,021 + -0.8 1,976,022 - -0.5 1,976,092 + +0.5 1,976,092 + -1.2 1,976,092 + -0.8 1,976,092 + +0.1 1,976,092 + +1.3 1,976,092 + -1.8 1,976,092 + +0.3 1,976,092 + -0.0 1,976,092 + +0.1 1,976,092 + -1.4 1,976,092 + -0.7 1,976,092 + -1.3 1,976,093 - -0.0 1,976,093 - -0.3 1,976,093 - -2.6 1,976,093 - -0.9 1,976,093 - +0.2 1,976,093 - +0.2 1,976,327 + Psest_1865 0.25 +0.8 1,976,327 + Psest_1865 0.25 +1.1 1,976,328 - Psest_1865 0.25 -1.7 1,976,379 + Psest_1865 0.32 -0.1 1,976,379 + Psest_1865 0.32 +0.8 1,976,379 + Psest_1865 0.32 -1.6 1,976,379 + Psest_1865 0.32 -0.2 1,976,380 - Psest_1865 0.32 +1.1 1,976,392 + Psest_1865 0.34 +0.5 1,976,393 - Psest_1865 0.34 -0.7 1,976,411 + Psest_1865 0.36 -1.0 1,976,420 + Psest_1865 0.38 -0.3 1,976,420 + Psest_1865 0.38 +0.2 1,976,420 + Psest_1865 0.38 +0.2 1,976,420 + Psest_1865 0.38 +1.4 1,976,420 + Psest_1865 0.38 +0.2 1,976,421 - Psest_1865 0.38 +1.1 1,976,421 - Psest_1865 0.38 -1.3 1,976,424 + Psest_1865 0.38 -0.5 1,976,424 + Psest_1865 0.38 +0.1 1,976,424 + Psest_1865 0.38 -0.0 1,976,424 + Psest_1865 0.38 +0.5 1,976,424 + Psest_1865 0.38 +2.0 1,976,424 + Psest_1865 0.38 -1.1 1,976,424 + Psest_1865 0.38 -0.6 1,976,424 + Psest_1865 0.38 -0.1 1,976,424 + Psest_1865 0.38 +0.7 1,976,424 + Psest_1865 0.38 +0.1 1,976,424 + Psest_1865 0.38 +2.1 1,976,424 + Psest_1865 0.38 -1.9 1,976,424 + Psest_1865 0.38 -0.8 1,976,425 - Psest_1865 0.38 -0.2 1,976,425 - Psest_1865 0.38 -1.6 1,976,425 - Psest_1865 0.38 +1.9 1,976,425 - Psest_1865 0.38 +0.3 1,976,425 - Psest_1865 0.38 -0.6 1,976,425 - Psest_1865 0.38 -2.9 1,976,425 - Psest_1865 0.38 +1.9 1,976,425 - Psest_1865 0.38 -0.5 1,976,425 - Psest_1865 0.38 -0.7 1,976,425 - Psest_1865 0.38 -0.4 1,976,425 - Psest_1865 0.38 +0.5 1,976,475 + Psest_1865 0.45 -0.0 1,976,475 + Psest_1865 0.45 -0.4 1,976,475 + Psest_1865 0.45 -0.6 1,976,475 + Psest_1865 0.45 +0.3 1,976,475 + Psest_1865 0.45 +1.0 1,976,475 + Psest_1865 0.45 -0.6 1,976,475 + Psest_1865 0.45 -0.4 1,976,475 + Psest_1865 0.45 -0.7 1,976,476 - Psest_1865 0.45 +0.3 1,976,476 - Psest_1865 0.45 +1.1 1,976,490 + Psest_1865 0.47 +1.7 1,976,491 - Psest_1865 0.48 -2.6 1,976,491 - Psest_1865 0.48 -0.7 1,976,544 + Psest_1865 0.55 +1.4 1,976,932 - +0.5 1,976,932 - -0.4 1,976,932 - +0.6 1,976,932 - -2.3 1,976,932 - -0.2 1,976,932 - -0.2 1,976,932 - -0.1 1,976,968 + Psest_1866 0.12 -0.5 1,976,968 + Psest_1866 0.12 -0.7 1,976,968 + Psest_1866 0.12 +0.8 1,976,968 + Psest_1866 0.12 +0.3 1,976,969 - Psest_1866 0.13 -3.1 1,976,969 - Psest_1866 0.13 -1.1 1,976,969 - Psest_1866 0.13 +1.7 1,976,969 - Psest_1866 0.13 +1.7 1,976,969 - Psest_1866 0.13 -0.8 1,976,969 - Psest_1866 0.13 +0.7 1,976,969 - Psest_1866 0.13 -1.6 1,977,073 + Psest_1866 0.37 +0.1 1,977,073 + Psest_1866 0.37 -0.2 1,977,073 + Psest_1866 0.37 -0.1 1,977,073 + Psest_1866 0.37 +0.3 1,977,073 + Psest_1866 0.37 +0.2 1,977,074 - Psest_1866 0.38 -0.1 1,977,074 - Psest_1866 0.38 +1.4
Or see this region's nucleotide sequence