Experiment: Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1423 and Psest_1424 are separated by 381 nucleotides Psest_1424 and Psest_1425 overlap by 4 nucleotides Psest_1425 and Psest_1426 are separated by 2 nucleotides Psest_1426 and Psest_1427 are separated by 5 nucleotides
Psest_1423: Psest_1423 - PQQ-dependent dehydrogenase, methanol/ethanol family, at 1,531,596 to 1,533,728
_1423
Psest_1424: Psest_1424 - Lipoate-protein ligase A, at 1,534,110 to 1,534,877
_1424
Psest_1425: Psest_1425 - Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes, at 1,534,874 to 1,535,575
_1425
Psest_1426: Psest_1426 - Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes, at 1,535,578 to 1,535,811
_1426
Psest_1427: Psest_1427 - Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit, at 1,535,817 to 1,536,839
_1427
Position (kb)
1534
1535 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1533.134 kb on + strand, within Psest_1423 at 1533.138 kb on + strand, within Psest_1423 at 1533.138 kb on + strand, within Psest_1423 at 1533.139 kb on - strand, within Psest_1423 at 1533.144 kb on + strand, within Psest_1423 at 1533.144 kb on + strand, within Psest_1423 at 1533.144 kb on + strand at 1533.145 kb on - strand, within Psest_1423 at 1533.145 kb on - strand, within Psest_1423 at 1533.145 kb on - strand, within Psest_1423 at 1533.145 kb on - strand, within Psest_1423 at 1533.280 kb on - strand, within Psest_1423 at 1533.280 kb on - strand, within Psest_1423 at 1533.280 kb on - strand, within Psest_1423 at 1533.280 kb on - strand, within Psest_1423 at 1533.312 kb on - strand, within Psest_1423 at 1533.436 kb on + strand, within Psest_1423 at 1533.436 kb on + strand, within Psest_1423 at 1533.436 kb on + strand, within Psest_1423 at 1533.436 kb on + strand, within Psest_1423 at 1533.436 kb on + strand, within Psest_1423 at 1533.436 kb on + strand, within Psest_1423 at 1533.436 kb on + strand, within Psest_1423 at 1533.437 kb on - strand, within Psest_1423 at 1533.439 kb on + strand, within Psest_1423 at 1533.439 kb on + strand, within Psest_1423 at 1533.439 kb on + strand, within Psest_1423 at 1533.439 kb on + strand, within Psest_1423 at 1533.440 kb on - strand, within Psest_1423 at 1533.440 kb on - strand, within Psest_1423 at 1533.534 kb on + strand at 1533.534 kb on + strand at 1533.534 kb on + strand at 1533.534 kb on + strand at 1533.535 kb on - strand at 1533.602 kb on + strand at 1533.636 kb on - strand at 1533.732 kb on + strand at 1533.817 kb on + strand at 1533.817 kb on + strand at 1533.875 kb on + strand at 1533.875 kb on + strand at 1533.876 kb on - strand at 1533.876 kb on - strand at 1533.876 kb on - strand at 1533.900 kb on - strand at 1533.903 kb on + strand at 1533.912 kb on - strand at 1533.913 kb on + strand at 1534.000 kb on + strand at 1534.000 kb on + strand at 1534.000 kb on + strand at 1534.001 kb on - strand at 1534.001 kb on - strand at 1534.001 kb on - strand at 1534.010 kb on + strand at 1534.107 kb on + strand at 1534.210 kb on + strand, within Psest_1424 at 1534.211 kb on - strand, within Psest_1424 at 1534.315 kb on + strand, within Psest_1424 at 1534.315 kb on + strand, within Psest_1424 at 1534.315 kb on + strand, within Psest_1424 at 1534.315 kb on + strand, within Psest_1424 at 1534.315 kb on + strand, within Psest_1424 at 1534.620 kb on - strand, within Psest_1424 at 1534.884 kb on + strand at 1534.894 kb on + strand at 1535.579 kb on + strand at 1535.811 kb on + strand at 1535.811 kb on + strand at 1535.812 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 200 mM remove 1,533,134 + Psest_1423 0.72 -1.4 1,533,138 + Psest_1423 0.72 -1.0 1,533,138 + Psest_1423 0.72 +1.1 1,533,139 - Psest_1423 0.72 +0.5 1,533,144 + Psest_1423 0.73 +0.9 1,533,144 + Psest_1423 0.73 +1.1 1,533,144 + +2.8 1,533,145 - Psest_1423 0.73 +0.2 1,533,145 - Psest_1423 0.73 +0.5 1,533,145 - Psest_1423 0.73 -0.6 1,533,145 - Psest_1423 0.73 +0.5 1,533,280 - Psest_1423 0.79 +3.1 1,533,280 - Psest_1423 0.79 +0.6 1,533,280 - Psest_1423 0.79 -1.8 1,533,280 - Psest_1423 0.79 +1.1 1,533,312 - Psest_1423 0.80 +1.5 1,533,436 + Psest_1423 0.86 -2.9 1,533,436 + Psest_1423 0.86 +0.5 1,533,436 + Psest_1423 0.86 -0.2 1,533,436 + Psest_1423 0.86 -1.1 1,533,436 + Psest_1423 0.86 +1.8 1,533,436 + Psest_1423 0.86 +1.2 1,533,436 + Psest_1423 0.86 +1.6 1,533,437 - Psest_1423 0.86 -2.8 1,533,439 + Psest_1423 0.86 +0.4 1,533,439 + Psest_1423 0.86 +1.5 1,533,439 + Psest_1423 0.86 +0.3 1,533,439 + Psest_1423 0.86 -1.6 1,533,440 - Psest_1423 0.86 -0.6 1,533,440 - Psest_1423 0.86 +1.4 1,533,534 + -1.3 1,533,534 + +1.3 1,533,534 + +0.9 1,533,534 + -2.1 1,533,535 - +2.1 1,533,602 + -0.0 1,533,636 - +0.4 1,533,732 + +0.2 1,533,817 + +0.1 1,533,817 + -0.9 1,533,875 + +0.1 1,533,875 + -1.2 1,533,876 - +0.0 1,533,876 - -0.5 1,533,876 - +0.3 1,533,900 - +0.5 1,533,903 + +1.9 1,533,912 - -0.4 1,533,913 + +0.2 1,534,000 + -1.8 1,534,000 + +0.3 1,534,000 + -1.9 1,534,001 - +2.0 1,534,001 - +1.5 1,534,001 - +0.7 1,534,010 + +0.2 1,534,107 + -0.6 1,534,210 + Psest_1424 0.13 +0.2 1,534,211 - Psest_1424 0.13 -1.8 1,534,315 + Psest_1424 0.27 +0.8 1,534,315 + Psest_1424 0.27 +0.0 1,534,315 + Psest_1424 0.27 +1.6 1,534,315 + Psest_1424 0.27 +1.7 1,534,315 + Psest_1424 0.27 +1.7 1,534,620 - Psest_1424 0.66 +0.3 1,534,884 + +1.2 1,534,894 + -0.2 1,535,579 + +0.3 1,535,811 + -0.3 1,535,811 + +2.1 1,535,812 - -2.3
Or see this region's nucleotide sequence