Experiment: perchlorate 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2297 and Psest_2298 are separated by 150 nucleotides Psest_2298 and Psest_2299 are separated by 51 nucleotides Psest_2299 and Psest_2300 are separated by 32 nucleotides Psest_2300 and Psest_2301 are separated by 273 nucleotides Psest_2301 and Psest_2302 are separated by 58 nucleotides
Psest_2297: Psest_2297 - hypothetical protein, at 2,448,974 to 2,450,401
_2297
Psest_2298: Psest_2298 - tRNA_Leu_TAG, at 2,450,552 to 2,450,636
_2298
Psest_2299: Psest_2299 - tRNA_His_GTG, at 2,450,688 to 2,450,763
_2299
Psest_2300: Psest_2300 - tRNA_Arg_TCT, at 2,450,796 to 2,450,872
_2300
Psest_2301: Psest_2301 - 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase, at 2,451,146 to 2,452,000
_2301
Psest_2302: Psest_2302 - cysteinyl-tRNA synthetase, at 2,452,059 to 2,453,447
_2302
Position (kb)
2451
2452
2453 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2450.184 kb on + strand, within Psest_2297 at 2450.185 kb on - strand, within Psest_2297 at 2450.452 kb on + strand at 2450.453 kb on - strand at 2450.456 kb on + strand at 2450.456 kb on + strand at 2450.457 kb on - strand at 2450.457 kb on - strand at 2450.529 kb on + strand at 2450.530 kb on - strand at 2450.530 kb on - strand at 2450.876 kb on - strand at 2451.037 kb on + strand at 2452.048 kb on + strand at 2452.048 kb on + strand at 2452.049 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction perchlorate 25 mM remove 2,450,184 + Psest_2297 0.85 +0.4 2,450,185 - Psest_2297 0.85 +1.8 2,450,452 + +1.6 2,450,453 - -0.1 2,450,456 + -2.9 2,450,456 + +1.7 2,450,457 - +1.7 2,450,457 - +0.1 2,450,529 + -2.8 2,450,530 - -0.4 2,450,530 - -1.3 2,450,876 - +0.1 2,451,037 + +2.0 2,452,048 + -0.8 2,452,048 + +0.1 2,452,049 - -0.3
Or see this region's nucleotide sequence