Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0277

Experiment: perchlorate 25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0276 and Psest_0277 are separated by 65 nucleotidesPsest_0277 and Psest_0278 overlap by 4 nucleotidesPsest_0278 and Psest_0279 are separated by 26 nucleotides Psest_0276: Psest_0276 - putative oxygen-independent coproporphyrinogen III oxidase, at 291,796 to 292,929 _0276 Psest_0277: Psest_0277 - non-canonical purine NTP pyrophosphatase, rdgB/HAM1 family, at 292,995 to 293,588 _0277 Psest_0278: Psest_0278 - hypothetical protein, at 293,585 to 294,007 _0278 Psest_0279: Psest_0279 - methionine biosynthesis protein MetW, at 294,034 to 294,633 _0279 Position (kb) 292 293 294Strain fitness (log2 ratio) -2 -1 0 1 2at 292.067 kb on + strand, within Psest_0276at 292.106 kb on + strand, within Psest_0276at 292.107 kb on - strand, within Psest_0276at 292.107 kb on - strand, within Psest_0276at 292.176 kb on - strand, within Psest_0276at 292.176 kb on - strand, within Psest_0276at 292.229 kb on + strand, within Psest_0276at 292.230 kb on - strand, within Psest_0276at 292.598 kb on + strand, within Psest_0276at 292.820 kb on + strandat 292.821 kb on - strandat 292.821 kb on - strandat 292.907 kb on + strandat 292.907 kb on + strandat 292.908 kb on - strandat 292.956 kb on + strandat 292.957 kb on - strandat 292.999 kb on - strandat 293.332 kb on + strand, within Psest_0277at 293.332 kb on + strand, within Psest_0277at 293.333 kb on - strand, within Psest_0277at 293.649 kb on - strand, within Psest_0278at 293.703 kb on + strand, within Psest_0278at 293.703 kb on + strand, within Psest_0278at 293.704 kb on - strand, within Psest_0278at 293.704 kb on - strand, within Psest_0278at 293.704 kb on - strand, within Psest_0278at 293.706 kb on + strand, within Psest_0278at 293.706 kb on + strand, within Psest_0278at 293.706 kb on + strand, within Psest_0278at 293.706 kb on + strand, within Psest_0278at 293.706 kb on + strand, within Psest_0278at 293.707 kb on - strand, within Psest_0278at 293.707 kb on - strand, within Psest_0278at 293.707 kb on - strand, within Psest_0278at 293.707 kb on - strand, within Psest_0278at 293.707 kb on - strand, within Psest_0278at 293.913 kb on + strand, within Psest_0278at 293.913 kb on + strand, within Psest_0278at 293.913 kb on + strand, within Psest_0278at 293.913 kb on + strand, within Psest_0278at 293.914 kb on - strand, within Psest_0278at 293.914 kb on - strand, within Psest_0278at 293.914 kb on - strand, within Psest_0278at 293.914 kb on - strand, within Psest_0278at 293.914 kb on - strand, within Psest_0278at 293.914 kb on - strand, within Psest_0278at 293.914 kb on - strand, within Psest_0278at 293.914 kb on - strand, within Psest_0278at 293.925 kb on + strand, within Psest_0278at 293.926 kb on - strand, within Psest_0278at 293.926 kb on - strand, within Psest_0278at 293.926 kb on - strand, within Psest_0278at 294.007 kb on + strandat 294.007 kb on + strandat 294.007 kb on + strandat 294.008 kb on - strandat 294.008 kb on - strandat 294.013 kb on + strandat 294.059 kb on + strandat 294.060 kb on - strandat 294.218 kb on + strand, within Psest_0279at 294.218 kb on + strand, within Psest_0279at 294.219 kb on - strand, within Psest_0279at 294.219 kb on - strand, within Psest_0279at 294.219 kb on - strand, within Psest_0279at 294.219 kb on - strand, within Psest_0279at 294.219 kb on - strand, within Psest_0279at 294.219 kb on - strand, within Psest_0279at 294.219 kb on - strand, within Psest_0279at 294.227 kb on + strand, within Psest_0279at 294.227 kb on + strand, within Psest_0279at 294.228 kb on - strand, within Psest_0279at 294.228 kb on - strand, within Psest_0279

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Per-strain Table

Position Strand Gene LocusTag Fraction perchlorate 25 mM
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292,067 + Psest_0276 0.24 +0.8
292,106 + Psest_0276 0.27 -0.3
292,107 - Psest_0276 0.27 +0.3
292,107 - Psest_0276 0.27 -0.3
292,176 - Psest_0276 0.34 -1.2
292,176 - Psest_0276 0.34 +0.1
292,229 + Psest_0276 0.38 -0.9
292,230 - Psest_0276 0.38 +0.7
292,598 + Psest_0276 0.71 -0.9
292,820 + -1.4
292,821 - -1.2
292,821 - +1.0
292,907 + -0.7
292,907 + -0.4
292,908 - -0.7
292,956 + -0.4
292,957 - +0.9
292,999 - +0.5
293,332 + Psest_0277 0.57 +1.8
293,332 + Psest_0277 0.57 +1.3
293,333 - Psest_0277 0.57 +1.3
293,649 - Psest_0278 0.15 +1.5
293,703 + Psest_0278 0.28 -0.3
293,703 + Psest_0278 0.28 -0.9
293,704 - Psest_0278 0.28 -2.6
293,704 - Psest_0278 0.28 -1.5
293,704 - Psest_0278 0.28 -1.0
293,706 + Psest_0278 0.29 -1.6
293,706 + Psest_0278 0.29 -0.7
293,706 + Psest_0278 0.29 +1.2
293,706 + Psest_0278 0.29 +0.3
293,706 + Psest_0278 0.29 -0.3
293,707 - Psest_0278 0.29 -1.1
293,707 - Psest_0278 0.29 -0.1
293,707 - Psest_0278 0.29 +0.3
293,707 - Psest_0278 0.29 +1.1
293,707 - Psest_0278 0.29 -0.4
293,913 + Psest_0278 0.78 +1.6
293,913 + Psest_0278 0.78 +1.1
293,913 + Psest_0278 0.78 +0.8
293,913 + Psest_0278 0.78 -0.1
293,914 - Psest_0278 0.78 -0.1
293,914 - Psest_0278 0.78 +0.4
293,914 - Psest_0278 0.78 -0.6
293,914 - Psest_0278 0.78 -0.8
293,914 - Psest_0278 0.78 -0.1
293,914 - Psest_0278 0.78 +1.1
293,914 - Psest_0278 0.78 +1.4
293,914 - Psest_0278 0.78 +0.8
293,925 + Psest_0278 0.80 -1.1
293,926 - Psest_0278 0.81 +0.1
293,926 - Psest_0278 0.81 +1.1
293,926 - Psest_0278 0.81 +0.3
294,007 + +1.2
294,007 + -1.8
294,007 + -1.2
294,008 - -0.0
294,008 - +1.4
294,013 + +0.0
294,059 + -0.8
294,060 - -0.6
294,218 + Psest_0279 0.31 +1.5
294,218 + Psest_0279 0.31 -0.1
294,219 - Psest_0279 0.31 -0.1
294,219 - Psest_0279 0.31 -0.6
294,219 - Psest_0279 0.31 +0.8
294,219 - Psest_0279 0.31 +0.3
294,219 - Psest_0279 0.31 +0.9
294,219 - Psest_0279 0.31 +0.4
294,219 - Psest_0279 0.31 +0.7
294,227 + Psest_0279 0.32 -1.0
294,227 + Psest_0279 0.32 -1.4
294,228 - Psest_0279 0.32 +0.1
294,228 - Psest_0279 0.32 -1.2

Or see this region's nucleotide sequence