Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1641

Experiment: perchlorate 25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1640 and Psest_1641 are separated by 160 nucleotidesPsest_1641 and Psest_1642 are separated by 114 nucleotidesPsest_1642 and Psest_1643 are separated by 108 nucleotides Psest_1640: Psest_1640 - (p)ppGpp synthetase, RelA/SpoT family, at 1,743,315 to 1,745,558 _1640 Psest_1641: Psest_1641 - MazG family protein, at 1,745,719 to 1,746,549 _1641 Psest_1642: Psest_1642 - Uncharacterized protein conserved in bacteria, at 1,746,664 to 1,747,203 _1642 Psest_1643: Psest_1643 - hypothetical protein, at 1,747,312 to 1,747,473 _1643 Position (kb) 1745 1746 1747Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1744.737 kb on - strand, within Psest_1640at 1744.737 kb on - strand, within Psest_1640at 1744.788 kb on + strand, within Psest_1640at 1744.788 kb on + strand, within Psest_1640at 1744.789 kb on - strand, within Psest_1640at 1744.789 kb on - strand, within Psest_1640at 1745.193 kb on + strand, within Psest_1640at 1745.193 kb on + strand, within Psest_1640at 1745.219 kb on + strand, within Psest_1640at 1745.369 kb on + strandat 1745.369 kb on + strandat 1745.369 kb on + strandat 1745.369 kb on + strandat 1745.556 kb on + strandat 1745.673 kb on + strandat 1745.673 kb on + strandat 1745.673 kb on + strandat 1745.722 kb on + strandat 1745.722 kb on + strandat 1745.722 kb on + strandat 1745.723 kb on - strandat 1745.723 kb on - strandat 1745.723 kb on - strandat 1745.804 kb on - strand, within Psest_1641at 1745.839 kb on + strand, within Psest_1641at 1745.840 kb on - strand, within Psest_1641at 1745.840 kb on - strand, within Psest_1641at 1745.840 kb on - strand, within Psest_1641at 1745.920 kb on + strand, within Psest_1641at 1745.920 kb on + strand, within Psest_1641at 1745.921 kb on - strand, within Psest_1641at 1745.923 kb on + strand, within Psest_1641at 1745.924 kb on - strand, within Psest_1641at 1746.034 kb on + strand, within Psest_1641at 1746.034 kb on + strand, within Psest_1641at 1746.035 kb on - strand, within Psest_1641at 1746.035 kb on - strand, within Psest_1641at 1746.035 kb on - strand, within Psest_1641at 1746.952 kb on + strand, within Psest_1642at 1746.953 kb on - strand, within Psest_1642at 1746.953 kb on - strand, within Psest_1642at 1746.955 kb on + strand, within Psest_1642at 1746.955 kb on + strand, within Psest_1642at 1746.956 kb on - strand, within Psest_1642at 1746.956 kb on - strand, within Psest_1642at 1746.956 kb on - strand, within Psest_1642at 1747.265 kb on + strandat 1747.336 kb on - strand, within Psest_1643at 1747.336 kb on - strand, within Psest_1643

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Per-strain Table

Position Strand Gene LocusTag Fraction perchlorate 25 mM
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1,744,737 - Psest_1640 0.63 -1.7
1,744,737 - Psest_1640 0.63 -2.9
1,744,788 + Psest_1640 0.66 -1.7
1,744,788 + Psest_1640 0.66 -0.7
1,744,789 - Psest_1640 0.66 -2.0
1,744,789 - Psest_1640 0.66 -2.2
1,745,193 + Psest_1640 0.84 -0.3
1,745,193 + Psest_1640 0.84 -0.8
1,745,219 + Psest_1640 0.85 +0.2
1,745,369 + -0.6
1,745,369 + -1.2
1,745,369 + +1.2
1,745,369 + -2.5
1,745,556 + -0.6
1,745,673 + -2.1
1,745,673 + -2.1
1,745,673 + +0.1
1,745,722 + +0.2
1,745,722 + -0.9
1,745,722 + +0.7
1,745,723 - -0.6
1,745,723 - -0.6
1,745,723 - +0.1
1,745,804 - Psest_1641 0.10 +0.8
1,745,839 + Psest_1641 0.14 +2.3
1,745,840 - Psest_1641 0.15 +0.4
1,745,840 - Psest_1641 0.15 +1.2
1,745,840 - Psest_1641 0.15 +0.9
1,745,920 + Psest_1641 0.24 +1.8
1,745,920 + Psest_1641 0.24 +0.2
1,745,921 - Psest_1641 0.24 +0.4
1,745,923 + Psest_1641 0.25 -2.3
1,745,924 - Psest_1641 0.25 +0.3
1,746,034 + Psest_1641 0.38 +1.4
1,746,034 + Psest_1641 0.38 +0.9
1,746,035 - Psest_1641 0.38 +0.9
1,746,035 - Psest_1641 0.38 +0.6
1,746,035 - Psest_1641 0.38 +0.4
1,746,952 + Psest_1642 0.53 +0.2
1,746,953 - Psest_1642 0.54 -1.1
1,746,953 - Psest_1642 0.54 -0.4
1,746,955 + Psest_1642 0.54 +2.2
1,746,955 + Psest_1642 0.54 -3.5
1,746,956 - Psest_1642 0.54 -1.4
1,746,956 - Psest_1642 0.54 -0.2
1,746,956 - Psest_1642 0.54 -0.1
1,747,265 + -1.2
1,747,336 - Psest_1643 0.15 +0.9
1,747,336 - Psest_1643 0.15 -0.1

Or see this region's nucleotide sequence