Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_3000

Experiment: perchlorate 12.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_2998 and Psest_2999 are separated by 61 nucleotidesPsest_2999 and Psest_3000 are separated by 4 nucleotidesPsest_3000 and Psest_3001 overlap by 4 nucleotides Psest_2998: Psest_2998 - Glutathione peroxidase, at 3,188,523 to 3,189,071 _2998 Psest_2999: Psest_2999 - molybdenum ABC transporter, periplasmic molybdate-binding protein, at 3,189,133 to 3,189,978 _2999 Psest_3000: Psest_3000 - molybdate ABC transporter, permease protein, at 3,189,983 to 3,190,663 _3000 Psest_3001: Psest_3001 - molybdenum ABC transporter, ATP-binding protein, at 3,190,660 to 3,191,757 _3001 Position (kb) 3189 3190 3191Strain fitness (log2 ratio) -1 0 1 2 3 4 5at 3189.066 kb on - strandat 3189.066 kb on - strandat 3189.103 kb on + strandat 3189.181 kb on + strandat 3189.181 kb on + strandat 3189.181 kb on + strandat 3189.181 kb on + strandat 3189.255 kb on - strand, within Psest_2999at 3189.296 kb on + strand, within Psest_2999at 3189.296 kb on + strand, within Psest_2999at 3189.296 kb on + strand, within Psest_2999at 3189.409 kb on + strand, within Psest_2999at 3189.520 kb on + strand, within Psest_2999at 3189.520 kb on + strand, within Psest_2999at 3189.520 kb on + strand, within Psest_2999at 3189.520 kb on + strand, within Psest_2999at 3189.520 kb on + strand, within Psest_2999at 3189.521 kb on - strand, within Psest_2999at 3189.521 kb on - strand, within Psest_2999at 3189.521 kb on - strand, within Psest_2999at 3189.521 kb on - strand, within Psest_2999at 3189.733 kb on + strand, within Psest_2999at 3189.976 kb on + strandat 3189.977 kb on - strandat 3190.099 kb on + strand, within Psest_3000at 3190.175 kb on + strand, within Psest_3000at 3190.175 kb on + strand, within Psest_3000at 3190.176 kb on - strand, within Psest_3000at 3190.176 kb on - strand, within Psest_3000at 3190.176 kb on - strand, within Psest_3000at 3190.176 kb on - strand, within Psest_3000at 3190.286 kb on + strand, within Psest_3000at 3190.286 kb on + strand, within Psest_3000at 3190.286 kb on + strand, within Psest_3000at 3190.286 kb on + strand, within Psest_3000at 3190.287 kb on - strand, within Psest_3000at 3190.287 kb on - strand, within Psest_3000at 3190.287 kb on - strand, within Psest_3000at 3190.368 kb on + strandat 3190.538 kb on + strand, within Psest_3000at 3190.538 kb on + strand, within Psest_3000at 3190.538 kb on + strand, within Psest_3000at 3190.538 kb on + strand, within Psest_3000at 3190.538 kb on + strand, within Psest_3000at 3190.538 kb on + strand, within Psest_3000at 3190.539 kb on - strand, within Psest_3000at 3190.539 kb on - strand, within Psest_3000at 3190.539 kb on - strand, within Psest_3000at 3190.539 kb on - strand, within Psest_3000at 3190.563 kb on - strand, within Psest_3000at 3190.629 kb on - strandat 3190.921 kb on + strand, within Psest_3001at 3190.922 kb on - strand, within Psest_3001at 3191.245 kb on + strand, within Psest_3001at 3191.245 kb on + strand, within Psest_3001at 3191.246 kb on - strand, within Psest_3001at 3191.246 kb on - strand, within Psest_3001at 3191.428 kb on + strand, within Psest_3001at 3191.429 kb on - strand, within Psest_3001at 3191.465 kb on + strand, within Psest_3001at 3191.466 kb on - strand, within Psest_3001at 3191.466 kb on - strand, within Psest_3001

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Per-strain Table

Position Strand Gene LocusTag Fraction perchlorate 12.5 mM
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3,189,066 - -1.1
3,189,066 - +2.2
3,189,103 + -0.5
3,189,181 + -0.3
3,189,181 + -1.0
3,189,181 + -1.1
3,189,181 + +0.3
3,189,255 - Psest_2999 0.14 +3.2
3,189,296 + Psest_2999 0.19 +3.8
3,189,296 + Psest_2999 0.19 +3.3
3,189,296 + Psest_2999 0.19 +0.6
3,189,409 + Psest_2999 0.33 +1.7
3,189,520 + Psest_2999 0.46 +2.2
3,189,520 + Psest_2999 0.46 +3.2
3,189,520 + Psest_2999 0.46 +1.3
3,189,520 + Psest_2999 0.46 +3.6
3,189,520 + Psest_2999 0.46 +2.7
3,189,521 - Psest_2999 0.46 +2.1
3,189,521 - Psest_2999 0.46 +3.8
3,189,521 - Psest_2999 0.46 +4.3
3,189,521 - Psest_2999 0.46 +1.0
3,189,733 + Psest_2999 0.71 +2.2
3,189,976 + +2.7
3,189,977 - +1.9
3,190,099 + Psest_3000 0.17 +3.1
3,190,175 + Psest_3000 0.28 +3.6
3,190,175 + Psest_3000 0.28 +3.9
3,190,176 - Psest_3000 0.28 +2.3
3,190,176 - Psest_3000 0.28 +3.8
3,190,176 - Psest_3000 0.28 +2.5
3,190,176 - Psest_3000 0.28 +5.4
3,190,286 + Psest_3000 0.44 +3.0
3,190,286 + Psest_3000 0.44 +2.6
3,190,286 + Psest_3000 0.44 +4.6
3,190,286 + Psest_3000 0.44 +3.7
3,190,287 - Psest_3000 0.45 +3.6
3,190,287 - Psest_3000 0.45 +1.8
3,190,287 - Psest_3000 0.45 +2.0
3,190,368 + +0.3
3,190,538 + Psest_3000 0.81 +3.0
3,190,538 + Psest_3000 0.81 +3.2
3,190,538 + Psest_3000 0.81 +2.8
3,190,538 + Psest_3000 0.81 +4.4
3,190,538 + Psest_3000 0.81 +3.3
3,190,538 + Psest_3000 0.81 +1.5
3,190,539 - Psest_3000 0.82 +1.7
3,190,539 - Psest_3000 0.82 +4.9
3,190,539 - Psest_3000 0.82 +2.9
3,190,539 - Psest_3000 0.82 +3.6
3,190,563 - Psest_3000 0.85 +5.0
3,190,629 - +4.7
3,190,921 + Psest_3001 0.24 +0.3
3,190,922 - Psest_3001 0.24 +3.5
3,191,245 + Psest_3001 0.53 +3.7
3,191,245 + Psest_3001 0.53 -1.3
3,191,246 - Psest_3001 0.53 +1.8
3,191,246 - Psest_3001 0.53 +3.4
3,191,428 + Psest_3001 0.70 +3.2
3,191,429 - Psest_3001 0.70 +2.7
3,191,465 + Psest_3001 0.73 +2.4
3,191,466 - Psest_3001 0.73 +4.1
3,191,466 - Psest_3001 0.73 +2.5

Or see this region's nucleotide sequence