Experiment: nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0085 and Psest_0086 are separated by 354 nucleotides Psest_0086 and Psest_0087 are separated by 71 nucleotides Psest_0087 and Psest_0088 are separated by 273 nucleotides
Psest_0085: Psest_0085 - alpha-ketoglutarate TRAP transporter, solute receptor component (from data) , at 87,238 to 88,191
_0085
Psest_0086: Psest_0086 - hypothetical protein, at 88,546 to 88,971
_0086
Psest_0087: Psest_0087 - hypothetical protein, at 89,043 to 89,285
_0087
Psest_0088: Psest_0088 - phosphonate metabolism protein PhnP, at 89,559 to 90,308
_0088
Position (kb)
89
90 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 88.073 kb on + strand, within Psest_0085 at 88.073 kb on + strand, within Psest_0085 at 88.074 kb on - strand, within Psest_0085 at 88.074 kb on - strand, within Psest_0085 at 88.074 kb on - strand, within Psest_0085 at 88.240 kb on - strand at 88.368 kb on + strand at 88.368 kb on + strand at 88.402 kb on - strand at 88.452 kb on + strand at 88.486 kb on + strand at 88.486 kb on + strand at 88.486 kb on + strand at 88.486 kb on + strand at 88.486 kb on + strand at 88.486 kb on + strand at 88.487 kb on - strand at 88.487 kb on - strand at 88.487 kb on - strand at 88.487 kb on - strand at 88.487 kb on - strand at 88.487 kb on - strand at 88.488 kb on + strand at 88.489 kb on - strand at 88.489 kb on - strand at 88.673 kb on - strand, within Psest_0086 at 88.769 kb on - strand, within Psest_0086 at 88.769 kb on - strand, within Psest_0086 at 88.769 kb on - strand, within Psest_0086 at 89.112 kb on + strand, within Psest_0087 at 89.112 kb on + strand, within Psest_0087 at 89.112 kb on + strand, within Psest_0087 at 89.113 kb on - strand, within Psest_0087 at 89.113 kb on - strand, within Psest_0087 at 89.429 kb on - strand at 89.460 kb on - strand at 89.560 kb on + strand at 89.561 kb on - strand at 89.561 kb on - strand at 89.561 kb on - strand at 89.801 kb on + strand, within Psest_0088 at 89.802 kb on - strand, within Psest_0088 at 89.834 kb on - strand, within Psest_0088 at 89.867 kb on - strand, within Psest_0088 at 89.983 kb on + strand, within Psest_0088 at 89.983 kb on + strand, within Psest_0088 at 89.984 kb on - strand, within Psest_0088 at 89.984 kb on - strand, within Psest_0088 at 89.984 kb on - strand, within Psest_0088 at 89.984 kb on - strand, within Psest_0088 at 90.259 kb on + strand at 90.259 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 200 mM remove 88,073 + Psest_0085 0.88 +1.0 88,073 + Psest_0085 0.88 -2.1 88,074 - Psest_0085 0.88 -1.0 88,074 - Psest_0085 0.88 +0.5 88,074 - Psest_0085 0.88 +1.5 88,240 - -0.4 88,368 + -1.4 88,368 + +2.1 88,402 - +1.1 88,452 + -3.2 88,486 + +1.1 88,486 + -0.0 88,486 + +2.5 88,486 + +1.3 88,486 + +0.5 88,486 + +2.0 88,487 - +1.0 88,487 - +0.1 88,487 - -0.6 88,487 - +0.6 88,487 - +2.8 88,487 - +1.4 88,488 + -0.0 88,489 - +0.3 88,489 - -0.8 88,673 - Psest_0086 0.30 -0.9 88,769 - Psest_0086 0.52 +1.7 88,769 - Psest_0086 0.52 -0.0 88,769 - Psest_0086 0.52 +1.8 89,112 + Psest_0087 0.28 +0.2 89,112 + Psest_0087 0.28 +0.1 89,112 + Psest_0087 0.28 +0.2 89,113 - Psest_0087 0.29 -1.4 89,113 - Psest_0087 0.29 -2.6 89,429 - +1.9 89,460 - -0.6 89,560 + +0.9 89,561 - -1.5 89,561 - -2.7 89,561 - +1.0 89,801 + Psest_0088 0.32 +0.6 89,802 - Psest_0088 0.32 -1.1 89,834 - Psest_0088 0.37 -1.0 89,867 - Psest_0088 0.41 +1.1 89,983 + Psest_0088 0.57 +1.7 89,983 + Psest_0088 0.57 +0.4 89,984 - Psest_0088 0.57 +2.1 89,984 - Psest_0088 0.57 -1.0 89,984 - Psest_0088 0.57 +1.7 89,984 - Psest_0088 0.57 +0.6 90,259 + +1.6 90,259 + -2.5
Or see this region's nucleotide sequence