Experiment: nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1054 and Psest_1055 are separated by 71 nucleotides Psest_1055 and Psest_1056 are separated by 348 nucleotides
Psest_1054: Psest_1054 - Diaminopimelate decarboxylase, at 1,142,571 to 1,143,668
_1054
Psest_1055: Psest_1055 - Saccharopine dehydrogenase and related proteins, at 1,143,740 to 1,144,936
_1055
Psest_1056: Psest_1056 - 2-isopropylmalate synthase, bacterial type, at 1,145,285 to 1,146,829
_1056
Position (kb)
1143
1144
1145 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1142.956 kb on - strand, within Psest_1054 at 1143.245 kb on - strand, within Psest_1054 at 1143.322 kb on - strand, within Psest_1054 at 1143.433 kb on - strand, within Psest_1054 at 1143.433 kb on - strand, within Psest_1054 at 1143.433 kb on - strand, within Psest_1054 at 1143.433 kb on - strand, within Psest_1054 at 1143.433 kb on - strand, within Psest_1054 at 1143.433 kb on - strand, within Psest_1054 at 1143.433 kb on - strand, within Psest_1054 at 1143.433 kb on - strand, within Psest_1054 at 1143.481 kb on - strand, within Psest_1054 at 1143.649 kb on - strand at 1143.649 kb on - strand at 1143.677 kb on + strand at 1143.678 kb on - strand at 1143.712 kb on + strand at 1143.712 kb on + strand at 1143.712 kb on + strand at 1143.712 kb on + strand at 1143.712 kb on + strand at 1143.712 kb on + strand at 1143.712 kb on + strand at 1143.713 kb on - strand at 1143.713 kb on - strand at 1143.751 kb on + strand at 1143.751 kb on + strand at 1143.751 kb on + strand at 1143.751 kb on + strand at 1143.752 kb on - strand at 1143.752 kb on - strand at 1143.790 kb on + strand at 1143.881 kb on + strand, within Psest_1055 at 1143.882 kb on - strand, within Psest_1055 at 1143.882 kb on - strand, within Psest_1055 at 1143.976 kb on + strand, within Psest_1055 at 1143.976 kb on + strand, within Psest_1055 at 1143.977 kb on - strand, within Psest_1055 at 1143.977 kb on - strand, within Psest_1055 at 1143.977 kb on - strand, within Psest_1055 at 1143.977 kb on - strand, within Psest_1055 at 1144.074 kb on - strand, within Psest_1055 at 1144.082 kb on + strand, within Psest_1055 at 1144.082 kb on - strand, within Psest_1055 at 1144.083 kb on - strand, within Psest_1055 at 1144.083 kb on - strand, within Psest_1055 at 1144.194 kb on - strand, within Psest_1055 at 1144.194 kb on - strand, within Psest_1055 at 1144.194 kb on - strand, within Psest_1055 at 1144.194 kb on - strand, within Psest_1055 at 1144.253 kb on + strand, within Psest_1055 at 1144.253 kb on + strand, within Psest_1055 at 1144.254 kb on - strand, within Psest_1055 at 1144.254 kb on - strand, within Psest_1055 at 1144.254 kb on - strand, within Psest_1055 at 1144.254 kb on - strand, within Psest_1055 at 1144.310 kb on + strand, within Psest_1055 at 1144.310 kb on + strand, within Psest_1055 at 1144.400 kb on + strand, within Psest_1055 at 1144.400 kb on + strand, within Psest_1055 at 1144.450 kb on - strand, within Psest_1055 at 1144.450 kb on - strand, within Psest_1055 at 1144.487 kb on + strand, within Psest_1055 at 1144.487 kb on + strand, within Psest_1055 at 1144.487 kb on + strand, within Psest_1055 at 1144.487 kb on + strand, within Psest_1055 at 1144.488 kb on - strand, within Psest_1055 at 1144.488 kb on - strand, within Psest_1055 at 1144.488 kb on - strand, within Psest_1055 at 1144.488 kb on - strand, within Psest_1055 at 1144.488 kb on - strand, within Psest_1055 at 1144.488 kb on - strand, within Psest_1055 at 1144.488 kb on - strand, within Psest_1055 at 1144.488 kb on - strand, within Psest_1055 at 1144.501 kb on + strand, within Psest_1055 at 1144.502 kb on - strand, within Psest_1055 at 1144.625 kb on + strand, within Psest_1055 at 1144.626 kb on - strand, within Psest_1055 at 1144.697 kb on + strand, within Psest_1055 at 1144.697 kb on + strand, within Psest_1055 at 1144.698 kb on - strand, within Psest_1055 at 1144.698 kb on - strand, within Psest_1055 at 1144.698 kb on - strand, within Psest_1055 at 1144.724 kb on + strand, within Psest_1055 at 1144.754 kb on + strand, within Psest_1055 at 1144.754 kb on + strand, within Psest_1055 at 1144.755 kb on - strand, within Psest_1055 at 1144.767 kb on + strand, within Psest_1055 at 1144.988 kb on + strand at 1144.996 kb on + strand at 1144.997 kb on - strand at 1144.997 kb on - strand at 1144.997 kb on - strand at 1144.997 kb on - strand at 1145.036 kb on + strand at 1145.037 kb on - strand at 1145.241 kb on + strand at 1145.242 kb on - strand at 1145.284 kb on + strand at 1145.615 kb on + strand, within Psest_1056 at 1145.615 kb on + strand, within Psest_1056 at 1145.828 kb on + strand, within Psest_1056
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 200 mM remove 1,142,956 - Psest_1054 0.35 +0.5 1,143,245 - Psest_1054 0.61 +0.3 1,143,322 - Psest_1054 0.68 +1.5 1,143,433 - Psest_1054 0.79 +0.6 1,143,433 - Psest_1054 0.79 +2.8 1,143,433 - Psest_1054 0.79 +1.3 1,143,433 - Psest_1054 0.79 +1.6 1,143,433 - Psest_1054 0.79 +0.2 1,143,433 - Psest_1054 0.79 +0.3 1,143,433 - Psest_1054 0.79 +0.1 1,143,433 - Psest_1054 0.79 +1.3 1,143,481 - Psest_1054 0.83 -0.1 1,143,649 - +2.0 1,143,649 - +0.8 1,143,677 + -0.7 1,143,678 - +0.8 1,143,712 + +1.7 1,143,712 + +3.3 1,143,712 + -1.3 1,143,712 + +0.6 1,143,712 + -0.6 1,143,712 + +0.7 1,143,712 + -0.1 1,143,713 - +1.0 1,143,713 - +0.2 1,143,751 + -0.5 1,143,751 + +1.3 1,143,751 + +1.4 1,143,751 + -1.0 1,143,752 - +1.1 1,143,752 - +0.2 1,143,790 + +0.9 1,143,881 + Psest_1055 0.12 +2.2 1,143,882 - Psest_1055 0.12 +2.0 1,143,882 - Psest_1055 0.12 -0.6 1,143,976 + Psest_1055 0.20 +2.1 1,143,976 + Psest_1055 0.20 +0.8 1,143,977 - Psest_1055 0.20 +0.0 1,143,977 - Psest_1055 0.20 -0.9 1,143,977 - Psest_1055 0.20 +0.6 1,143,977 - Psest_1055 0.20 -0.9 1,144,074 - Psest_1055 0.28 +1.0 1,144,082 + Psest_1055 0.29 -1.9 1,144,082 - Psest_1055 0.29 +1.9 1,144,083 - Psest_1055 0.29 +1.2 1,144,083 - Psest_1055 0.29 +0.4 1,144,194 - Psest_1055 0.38 +2.1 1,144,194 - Psest_1055 0.38 +3.1 1,144,194 - Psest_1055 0.38 +1.3 1,144,194 - Psest_1055 0.38 +0.3 1,144,253 + Psest_1055 0.43 +1.2 1,144,253 + Psest_1055 0.43 +0.9 1,144,254 - Psest_1055 0.43 +0.1 1,144,254 - Psest_1055 0.43 +1.8 1,144,254 - Psest_1055 0.43 +1.1 1,144,254 - Psest_1055 0.43 -2.2 1,144,310 + Psest_1055 0.48 +1.9 1,144,310 + Psest_1055 0.48 +1.2 1,144,400 + Psest_1055 0.55 +2.2 1,144,400 + Psest_1055 0.55 -1.5 1,144,450 - Psest_1055 0.59 +3.7 1,144,450 - Psest_1055 0.59 -0.7 1,144,487 + Psest_1055 0.62 +1.6 1,144,487 + Psest_1055 0.62 +0.3 1,144,487 + Psest_1055 0.62 +0.2 1,144,487 + Psest_1055 0.62 -1.9 1,144,488 - Psest_1055 0.62 +0.7 1,144,488 - Psest_1055 0.62 +0.9 1,144,488 - Psest_1055 0.62 +0.4 1,144,488 - Psest_1055 0.62 +0.2 1,144,488 - Psest_1055 0.62 -0.5 1,144,488 - Psest_1055 0.62 +1.2 1,144,488 - Psest_1055 0.62 +0.1 1,144,488 - Psest_1055 0.62 +3.1 1,144,501 + Psest_1055 0.64 +0.2 1,144,502 - Psest_1055 0.64 +3.0 1,144,625 + Psest_1055 0.74 +0.6 1,144,626 - Psest_1055 0.74 +0.7 1,144,697 + Psest_1055 0.80 +1.1 1,144,697 + Psest_1055 0.80 +0.2 1,144,698 - Psest_1055 0.80 +0.9 1,144,698 - Psest_1055 0.80 +1.5 1,144,698 - Psest_1055 0.80 +2.8 1,144,724 + Psest_1055 0.82 +0.5 1,144,754 + Psest_1055 0.85 +1.2 1,144,754 + Psest_1055 0.85 +1.4 1,144,755 - Psest_1055 0.85 +0.0 1,144,767 + Psest_1055 0.86 -0.3 1,144,988 + +1.5 1,144,996 + -0.8 1,144,997 - -1.1 1,144,997 - -1.7 1,144,997 - -1.6 1,144,997 - +1.6 1,145,036 + -2.9 1,145,037 - -0.5 1,145,241 + -2.6 1,145,242 - -0.9 1,145,284 + -1.0 1,145,615 + Psest_1056 0.21 +2.0 1,145,615 + Psest_1056 0.21 +0.8 1,145,828 + Psest_1056 0.35 +1.4
Or see this region's nucleotide sequence