Experiment: nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1242 and Psest_1243 are separated by 42 nucleotides Psest_1243 and Psest_1244 are separated by 64 nucleotides Psest_1244 and Psest_1245 are separated by 12 nucleotides
Psest_1242: Psest_1242 - tRNA-guanine transglycosylase, queuosine-34-forming, at 1,350,083 to 1,351,216
_1242
Psest_1243: Psest_1243 - preprotein translocase, YajC subunit, at 1,351,259 to 1,351,588
_1243
Psest_1244: Psest_1244 - protein-export membrane protein, SecD/SecF family, at 1,351,653 to 1,353,521
_1244
Psest_1245: Psest_1245 - protein-export membrane protein, SecD/SecF family, at 1,353,534 to 1,354,445
_1245
Position (kb)
1351
1352
1353
1354 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1350.661 kb on + strand, within Psest_1242 at 1351.089 kb on + strand, within Psest_1242 at 1351.089 kb on + strand, within Psest_1242 at 1351.089 kb on + strand, within Psest_1242 at 1351.093 kb on + strand, within Psest_1242 at 1351.150 kb on + strand at 1351.150 kb on + strand at 1351.178 kb on + strand at 1351.638 kb on + strand at 1353.519 kb on + strand at 1354.417 kb on + strand at 1354.417 kb on + strand at 1354.417 kb on + strand at 1354.417 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 200 mM remove 1,350,661 + Psest_1242 0.51 -1.2 1,351,089 + Psest_1242 0.89 +0.6 1,351,089 + Psest_1242 0.89 -0.0 1,351,089 + Psest_1242 0.89 -2.7 1,351,093 + Psest_1242 0.89 +3.2 1,351,150 + +1.0 1,351,150 + -2.5 1,351,178 + -0.5 1,351,638 + +0.3 1,353,519 + +2.0 1,354,417 + -2.0 1,354,417 + -1.5 1,354,417 + +2.2 1,354,417 + +2.3
Or see this region's nucleotide sequence