Experiment: nitrite 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1000 and Psest_1001 overlap by 17 nucleotides Psest_1001 and Psest_1002 are separated by 108 nucleotides Psest_1002 and Psest_1003 are separated by 89 nucleotides Psest_1003 and Psest_1004 are separated by 191 nucleotides
Psest_1000: Psest_1000 - PAS domain S-box, at 1,084,079 to 1,087,033
_1000
Psest_1001: Psest_1001 - hypothetical protein, at 1,087,017 to 1,087,193
_1001
Psest_1002: Psest_1002 - glutamyl-queuosine tRNA(Asp) synthetase, at 1,087,302 to 1,088,207
_1002
Psest_1003: Psest_1003 - RNA polymerase-binding protein DksA, at 1,088,297 to 1,088,743
_1003
Psest_1004: Psest_1004 - Aspartate/tyrosine/aromatic aminotransferase, at 1,088,935 to 1,090,107
_1004
Position (kb)
1087
1088
1089 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1086.334 kb on + strand, within Psest_1000 at 1086.335 kb on - strand, within Psest_1000 at 1086.409 kb on - strand, within Psest_1000 at 1086.409 kb on - strand, within Psest_1000 at 1086.409 kb on - strand, within Psest_1000 at 1086.409 kb on - strand, within Psest_1000 at 1086.556 kb on - strand, within Psest_1000 at 1086.738 kb on + strand at 1086.739 kb on - strand at 1086.766 kb on - strand at 1086.810 kb on + strand at 1086.811 kb on - strand at 1086.811 kb on - strand at 1086.811 kb on - strand at 1086.811 kb on - strand at 1086.811 kb on - strand at 1086.811 kb on - strand at 1086.841 kb on - strand at 1086.862 kb on - strand at 1086.882 kb on + strand at 1086.882 kb on + strand at 1086.883 kb on - strand at 1086.903 kb on + strand at 1086.904 kb on - strand at 1086.904 kb on - strand at 1086.904 kb on - strand at 1087.088 kb on - strand, within Psest_1001 at 1087.150 kb on + strand, within Psest_1001 at 1087.150 kb on + strand, within Psest_1001 at 1087.150 kb on + strand, within Psest_1001 at 1087.150 kb on + strand, within Psest_1001 at 1087.150 kb on + strand, within Psest_1001 at 1087.151 kb on - strand, within Psest_1001 at 1087.183 kb on + strand at 1087.184 kb on - strand at 1087.184 kb on - strand at 1087.187 kb on + strand at 1087.187 kb on + strand at 1087.187 kb on + strand at 1087.187 kb on + strand at 1087.187 kb on + strand at 1087.187 kb on + strand at 1087.187 kb on + strand at 1087.187 kb on + strand at 1087.188 kb on - strand at 1087.188 kb on - strand at 1087.188 kb on - strand at 1087.188 kb on - strand at 1087.188 kb on - strand at 1087.188 kb on - strand at 1087.188 kb on - strand at 1087.188 kb on - strand at 1087.188 kb on - strand at 1087.188 kb on - strand at 1087.188 kb on - strand at 1087.188 kb on - strand at 1087.188 kb on - strand at 1087.188 kb on - strand at 1087.188 kb on - strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.189 kb on + strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.190 kb on - strand at 1087.225 kb on + strand at 1087.492 kb on + strand, within Psest_1002 at 1087.582 kb on + strand, within Psest_1002 at 1087.582 kb on + strand, within Psest_1002 at 1087.771 kb on + strand, within Psest_1002 at 1087.886 kb on + strand, within Psest_1002 at 1087.886 kb on + strand, within Psest_1002 at 1087.886 kb on + strand, within Psest_1002 at 1087.887 kb on - strand, within Psest_1002 at 1087.887 kb on - strand, within Psest_1002 at 1087.887 kb on - strand, within Psest_1002 at 1087.894 kb on + strand, within Psest_1002 at 1087.894 kb on + strand, within Psest_1002 at 1087.895 kb on - strand, within Psest_1002 at 1087.895 kb on - strand, within Psest_1002 at 1087.895 kb on - strand, within Psest_1002 at 1087.990 kb on + strand, within Psest_1002 at 1087.991 kb on - strand, within Psest_1002 at 1087.991 kb on - strand, within Psest_1002 at 1088.101 kb on + strand, within Psest_1002 at 1088.102 kb on - strand, within Psest_1002 at 1088.102 kb on - strand, within Psest_1002 at 1088.686 kb on - strand, within Psest_1003 at 1088.686 kb on - strand, within Psest_1003 at 1088.901 kb on - strand at 1088.947 kb on + strand at 1088.947 kb on + strand at 1088.947 kb on + strand at 1088.947 kb on + strand at 1088.947 kb on + strand at 1088.947 kb on + strand at 1088.947 kb on + strand at 1088.947 kb on + strand at 1088.948 kb on - strand at 1088.948 kb on - strand at 1088.948 kb on - strand at 1088.948 kb on - strand at 1089.128 kb on - strand, within Psest_1004 at 1089.130 kb on - strand, within Psest_1004 at 1089.197 kb on + strand, within Psest_1004 at 1089.197 kb on + strand, within Psest_1004 at 1089.197 kb on + strand, within Psest_1004 at 1089.198 kb on - strand, within Psest_1004 at 1089.198 kb on - strand, within Psest_1004 at 1089.198 kb on - strand, within Psest_1004 at 1089.198 kb on - strand, within Psest_1004 at 1089.202 kb on - strand, within Psest_1004 at 1089.202 kb on - strand, within Psest_1004
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 2.5 mM remove 1,086,334 + Psest_1000 0.76 +1.8 1,086,335 - Psest_1000 0.76 -1.0 1,086,409 - Psest_1000 0.79 -0.7 1,086,409 - Psest_1000 0.79 -1.6 1,086,409 - Psest_1000 0.79 -1.3 1,086,409 - Psest_1000 0.79 -1.0 1,086,556 - Psest_1000 0.84 -1.3 1,086,738 + -1.9 1,086,739 - +0.4 1,086,766 - -0.8 1,086,810 + +0.2 1,086,811 - +1.8 1,086,811 - +0.0 1,086,811 - -2.1 1,086,811 - -0.3 1,086,811 - +0.3 1,086,811 - +0.3 1,086,841 - +0.7 1,086,862 - +0.7 1,086,882 + +2.7 1,086,882 + -0.1 1,086,883 - +0.5 1,086,903 + -1.3 1,086,904 - +0.2 1,086,904 - +0.4 1,086,904 - +0.5 1,087,088 - Psest_1001 0.40 +0.4 1,087,150 + Psest_1001 0.75 -1.0 1,087,150 + Psest_1001 0.75 -0.1 1,087,150 + Psest_1001 0.75 +1.8 1,087,150 + Psest_1001 0.75 +0.2 1,087,150 + Psest_1001 0.75 +1.0 1,087,151 - Psest_1001 0.76 -0.0 1,087,183 + +1.5 1,087,184 - +2.3 1,087,184 - -0.6 1,087,187 + +0.1 1,087,187 + +2.7 1,087,187 + -0.3 1,087,187 + -1.3 1,087,187 + +0.8 1,087,187 + +0.9 1,087,187 + +2.3 1,087,187 + +0.7 1,087,188 - +4.2 1,087,188 - -0.4 1,087,188 - -2.1 1,087,188 - -0.4 1,087,188 - -2.2 1,087,188 - +0.6 1,087,188 - +0.3 1,087,188 - -1.6 1,087,188 - -1.7 1,087,188 - +1.9 1,087,188 - -0.9 1,087,188 - -0.4 1,087,188 - +0.6 1,087,188 - +1.7 1,087,188 - -0.9 1,087,189 + +0.1 1,087,189 + -1.5 1,087,189 + +1.1 1,087,189 + +3.0 1,087,189 + +2.4 1,087,189 + +2.5 1,087,189 + -0.3 1,087,189 + +2.9 1,087,189 + +2.5 1,087,189 + -0.5 1,087,189 + -0.3 1,087,189 + +2.0 1,087,189 + +1.2 1,087,189 + +3.9 1,087,189 + +1.5 1,087,189 + +1.0 1,087,189 + +0.6 1,087,189 + +0.1 1,087,189 + -0.9 1,087,189 + +3.8 1,087,189 + +0.2 1,087,189 + -0.1 1,087,189 + -2.9 1,087,189 + +2.2 1,087,189 + +0.0 1,087,189 + +0.5 1,087,189 + -0.3 1,087,189 + +0.3 1,087,189 + +1.9 1,087,189 + +1.2 1,087,189 + +2.7 1,087,189 + +1.2 1,087,189 + +1.5 1,087,189 + +0.5 1,087,189 + +2.1 1,087,189 + -1.6 1,087,189 + -0.9 1,087,189 + +1.2 1,087,189 + +0.4 1,087,189 + +3.3 1,087,189 + -1.2 1,087,189 + +0.4 1,087,189 + +1.0 1,087,189 + +0.3 1,087,189 + +2.4 1,087,189 + +1.9 1,087,189 + +1.2 1,087,189 + +2.4 1,087,189 + +1.8 1,087,189 + -1.2 1,087,189 + +3.3 1,087,189 + +1.1 1,087,189 + +5.4 1,087,189 + +0.7 1,087,189 + +1.1 1,087,189 + +1.1 1,087,189 + +2.4 1,087,189 + -1.3 1,087,189 + +2.1 1,087,189 + +1.6 1,087,189 + -0.7 1,087,189 + +2.6 1,087,189 + +1.9 1,087,189 + +0.6 1,087,189 + +1.5 1,087,189 + +0.7 1,087,189 + +2.3 1,087,189 + +2.2 1,087,189 + +1.6 1,087,189 + +0.6 1,087,189 + +2.2 1,087,189 + +2.2 1,087,189 + -1.8 1,087,189 + +0.7 1,087,189 + +0.2 1,087,190 - -0.3 1,087,190 - -0.1 1,087,190 - -0.6 1,087,190 - +0.9 1,087,190 - +0.2 1,087,190 - -0.6 1,087,190 - +1.0 1,087,190 - +0.7 1,087,190 - -1.1 1,087,190 - +0.3 1,087,190 - +1.1 1,087,190 - -0.8 1,087,190 - -2.7 1,087,190 - +1.3 1,087,190 - +0.7 1,087,190 - -1.0 1,087,190 - -0.2 1,087,190 - -0.1 1,087,190 - -0.1 1,087,190 - -0.5 1,087,190 - -1.9 1,087,190 - -2.5 1,087,190 - +0.6 1,087,190 - -0.3 1,087,190 - -1.1 1,087,190 - +1.9 1,087,190 - -1.8 1,087,190 - -2.2 1,087,190 - -0.5 1,087,190 - +0.2 1,087,190 - -1.4 1,087,190 - +0.5 1,087,190 - +0.2 1,087,190 - -0.7 1,087,190 - -2.3 1,087,190 - -0.2 1,087,190 - -1.3 1,087,190 - +0.5 1,087,190 - +0.5 1,087,190 - -0.2 1,087,190 - -1.0 1,087,190 - -0.9 1,087,190 - +1.2 1,087,190 - -1.0 1,087,190 - -0.2 1,087,190 - +0.0 1,087,190 - +0.9 1,087,190 - -0.8 1,087,190 - +1.0 1,087,190 - -1.8 1,087,190 - +1.4 1,087,190 - -1.3 1,087,190 - -0.6 1,087,190 - -1.2 1,087,190 - -0.6 1,087,190 - +0.4 1,087,190 - -0.5 1,087,190 - -1.3 1,087,190 - -2.9 1,087,190 - -0.3 1,087,190 - +1.1 1,087,225 + -2.6 1,087,492 + Psest_1002 0.21 -1.0 1,087,582 + Psest_1002 0.31 -0.1 1,087,582 + Psest_1002 0.31 -1.4 1,087,771 + Psest_1002 0.52 +2.1 1,087,886 + Psest_1002 0.64 +2.0 1,087,886 + Psest_1002 0.64 +0.8 1,087,886 + Psest_1002 0.64 +1.7 1,087,887 - Psest_1002 0.65 +2.6 1,087,887 - Psest_1002 0.65 +0.2 1,087,887 - Psest_1002 0.65 -0.0 1,087,894 + Psest_1002 0.65 -1.2 1,087,894 + Psest_1002 0.65 +1.3 1,087,895 - Psest_1002 0.65 +1.8 1,087,895 - Psest_1002 0.65 -0.5 1,087,895 - Psest_1002 0.65 +1.0 1,087,990 + Psest_1002 0.76 -0.7 1,087,991 - Psest_1002 0.76 +1.2 1,087,991 - Psest_1002 0.76 -1.0 1,088,101 + Psest_1002 0.88 -0.9 1,088,102 - Psest_1002 0.88 +1.0 1,088,102 - Psest_1002 0.88 +0.7 1,088,686 - Psest_1003 0.87 +2.2 1,088,686 - Psest_1003 0.87 +0.5 1,088,901 - +0.9 1,088,947 + +0.8 1,088,947 + +0.5 1,088,947 + -0.4 1,088,947 + +0.3 1,088,947 + +0.1 1,088,947 + +1.1 1,088,947 + +0.8 1,088,947 + -0.3 1,088,948 - -0.1 1,088,948 - -2.2 1,088,948 - -1.3 1,088,948 - +1.4 1,089,128 - Psest_1004 0.16 +0.8 1,089,130 - Psest_1004 0.17 +0.8 1,089,197 + Psest_1004 0.22 -2.2 1,089,197 + Psest_1004 0.22 -2.3 1,089,197 + Psest_1004 0.22 -1.8 1,089,198 - Psest_1004 0.22 -2.3 1,089,198 - Psest_1004 0.22 -1.0 1,089,198 - Psest_1004 0.22 -0.2 1,089,198 - Psest_1004 0.22 -2.6 1,089,202 - Psest_1004 0.23 -0.5 1,089,202 - Psest_1004 0.23 +0.2
Or see this region's nucleotide sequence