Experiment: H2 N-free with 10mM L-Lysine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07500 and MMP_RS07505 are separated by 4 nucleotides MMP_RS07505 and MMP_RS07510 are separated by 15 nucleotides MMP_RS07510 and MMP_RS07515 are separated by 22 nucleotides MMP_RS07515 and MMP_RS07520 are separated by 14 nucleotides
MMP_RS07500: MMP_RS07500 - hypothetical protein, at 1,422,581 to 1,422,832
_RS07500
MMP_RS07505: MMP_RS07505 - DUF2104 domain-containing protein, at 1,422,837 to 1,423,136
_RS07505
MMP_RS07510: MMP_RS07510 - DUF1959 domain-containing protein, at 1,423,152 to 1,423,562
_RS07510
MMP_RS07515: MMP_RS07515 - NADH-quinone oxidoreductase subunit B family protein, at 1,423,585 to 1,424,055
_RS07515
MMP_RS07520: MMP_RS07520 - nickel-dependent hydrogenase large subunit, at 1,424,070 to 1,425,197
_RS07520
Position (kb)
1423
1424
1425 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1422.591 kb on + strand at 1422.809 kb on - strand at 1422.969 kb on + strand at 1423.108 kb on + strand at 1423.130 kb on + strand at 1423.560 kb on - strand at 1423.561 kb on + strand at 1423.881 kb on - strand at 1424.077 kb on - strand at 1424.179 kb on + strand at 1424.268 kb on - strand at 1424.268 kb on - strand at 1424.345 kb on + strand at 1424.463 kb on + strand at 1424.543 kb on + strand at 1424.946 kb on - strand at 1424.973 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Lysine remove 1,422,591 + -4.8 1,422,809 - -3.0 1,422,969 + -3.1 1,423,108 + -4.9 1,423,130 + -3.6 1,423,560 - -3.9 1,423,561 + -5.4 1,423,881 - -1.0 1,424,077 - -2.2 1,424,179 + -3.9 1,424,268 - -2.8 1,424,268 - -4.1 1,424,345 + -4.6 1,424,463 + -3.1 1,424,543 + -5.1 1,424,946 - -4.4 1,424,973 - -2.0
Or see this region's nucleotide sequence