Experiment: H2 N-free with 10mM L-Arginine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07520 and MMP_RS07525 are separated by 28 nucleotides MMP_RS07525 and MMP_RS07530 overlap by 8 nucleotides MMP_RS07530 and MMP_RS07535 are separated by 20 nucleotides
MMP_RS07520: MMP_RS07520 - nickel-dependent hydrogenase large subunit, at 1,424,070 to 1,425,197
_RS07520
MMP_RS07525: MMP_RS07525 - 4Fe-4S binding protein, at 1,425,226 to 1,426,407
_RS07525
MMP_RS07530: MMP_RS07530 - 4Fe-4S binding protein, at 1,426,400 to 1,426,885
_RS07530
MMP_RS07535: MMP_RS07535 - 4Fe-4S binding protein, at 1,426,906 to 1,427,664
_RS07535
Position (kb)
1425
1426
1427 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1424.268 kb on - strand at 1424.268 kb on - strand at 1424.345 kb on + strand at 1424.463 kb on + strand at 1424.543 kb on + strand at 1424.946 kb on - strand at 1424.973 kb on - strand at 1425.060 kb on - strand at 1425.060 kb on - strand at 1425.068 kb on + strand at 1425.105 kb on - strand at 1425.105 kb on - strand at 1425.105 kb on - strand at 1425.107 kb on - strand at 1425.180 kb on + strand at 1425.187 kb on + strand at 1425.198 kb on - strand at 1425.203 kb on + strand at 1425.203 kb on + strand at 1425.221 kb on - strand at 1425.225 kb on - strand at 1425.225 kb on - strand at 1425.225 kb on - strand at 1425.225 kb on - strand at 1425.226 kb on + strand at 1425.226 kb on + strand at 1425.226 kb on + strand at 1425.226 kb on + strand at 1425.315 kb on - strand at 1425.372 kb on - strand, within MMP_RS07525 at 1425.479 kb on - strand, within MMP_RS07525 at 1425.587 kb on - strand, within MMP_RS07525 at 1425.588 kb on + strand, within MMP_RS07525 at 1425.591 kb on + strand, within MMP_RS07525 at 1425.854 kb on - strand, within MMP_RS07525 at 1425.945 kb on - strand, within MMP_RS07525 at 1425.946 kb on + strand, within MMP_RS07525 at 1425.961 kb on + strand, within MMP_RS07525 at 1426.004 kb on - strand, within MMP_RS07525 at 1426.051 kb on - strand, within MMP_RS07525 at 1426.095 kb on - strand, within MMP_RS07525 at 1426.095 kb on - strand, within MMP_RS07525 at 1426.105 kb on + strand, within MMP_RS07525 at 1426.131 kb on - strand, within MMP_RS07525 at 1426.219 kb on - strand, within MMP_RS07525 at 1426.325 kb on - strand at 1426.325 kb on - strand at 1426.518 kb on - strand, within MMP_RS07530 at 1426.518 kb on - strand, within MMP_RS07530 at 1426.519 kb on + strand, within MMP_RS07530 at 1426.519 kb on + strand, within MMP_RS07530 at 1426.519 kb on + strand, within MMP_RS07530 at 1426.519 kb on + strand, within MMP_RS07530 at 1426.522 kb on - strand, within MMP_RS07530 at 1426.523 kb on + strand, within MMP_RS07530 at 1426.523 kb on + strand, within MMP_RS07530 at 1426.523 kb on + strand, within MMP_RS07530 at 1426.523 kb on + strand, within MMP_RS07530 at 1426.523 kb on + strand, within MMP_RS07530 at 1426.526 kb on + strand, within MMP_RS07530 at 1426.526 kb on + strand, within MMP_RS07530 at 1426.526 kb on + strand, within MMP_RS07530 at 1426.594 kb on - strand, within MMP_RS07530 at 1426.743 kb on - strand, within MMP_RS07530 at 1426.744 kb on + strand, within MMP_RS07530 at 1426.886 kb on - strand at 1426.947 kb on - strand at 1427.029 kb on - strand, within MMP_RS07535 at 1427.053 kb on + strand, within MMP_RS07535 at 1427.228 kb on - strand, within MMP_RS07535 at 1427.229 kb on + strand, within MMP_RS07535 at 1427.256 kb on + strand, within MMP_RS07535 at 1427.380 kb on + strand, within MMP_RS07535 at 1427.381 kb on - strand, within MMP_RS07535 at 1427.381 kb on - strand, within MMP_RS07535 at 1427.382 kb on + strand, within MMP_RS07535
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Arginine remove 1,424,268 - -2.3 1,424,268 - -4.6 1,424,345 + -3.1 1,424,463 + -5.2 1,424,543 + -3.6 1,424,946 - -4.9 1,424,973 - -3.5 1,425,060 - -2.5 1,425,060 - -3.8 1,425,068 + -2.9 1,425,105 - -1.9 1,425,105 - -2.5 1,425,105 - -5.2 1,425,107 - -4.5 1,425,180 + -2.5 1,425,187 + -1.9 1,425,198 - -6.8 1,425,203 + -6.6 1,425,203 + -2.5 1,425,221 - -5.0 1,425,225 - -2.9 1,425,225 - -3.7 1,425,225 - -1.9 1,425,225 - -4.8 1,425,226 + -3.1 1,425,226 + -2.8 1,425,226 + -5.2 1,425,226 + -6.4 1,425,315 - -6.3 1,425,372 - MMP_RS07525 0.12 -2.8 1,425,479 - MMP_RS07525 0.21 -3.1 1,425,587 - MMP_RS07525 0.31 -6.2 1,425,588 + MMP_RS07525 0.31 -4.0 1,425,591 + MMP_RS07525 0.31 -6.1 1,425,854 - MMP_RS07525 0.53 -3.9 1,425,945 - MMP_RS07525 0.61 -3.3 1,425,946 + MMP_RS07525 0.61 -5.0 1,425,961 + MMP_RS07525 0.62 -4.2 1,426,004 - MMP_RS07525 0.66 -5.1 1,426,051 - MMP_RS07525 0.70 -3.9 1,426,095 - MMP_RS07525 0.74 -4.3 1,426,095 - MMP_RS07525 0.74 -5.4 1,426,105 + MMP_RS07525 0.74 -3.8 1,426,131 - MMP_RS07525 0.77 -2.5 1,426,219 - MMP_RS07525 0.84 -1.3 1,426,325 - -3.0 1,426,325 - -2.1 1,426,518 - MMP_RS07530 0.24 -1.9 1,426,518 - MMP_RS07530 0.24 -2.1 1,426,519 + MMP_RS07530 0.24 -1.7 1,426,519 + MMP_RS07530 0.24 -0.5 1,426,519 + MMP_RS07530 0.24 -1.5 1,426,519 + MMP_RS07530 0.24 -1.9 1,426,522 - MMP_RS07530 0.25 -4.1 1,426,523 + MMP_RS07530 0.25 -1.0 1,426,523 + MMP_RS07530 0.25 -2.6 1,426,523 + MMP_RS07530 0.25 -1.1 1,426,523 + MMP_RS07530 0.25 -1.2 1,426,523 + MMP_RS07530 0.25 -2.0 1,426,526 + MMP_RS07530 0.26 -0.6 1,426,526 + MMP_RS07530 0.26 -0.4 1,426,526 + MMP_RS07530 0.26 -1.4 1,426,594 - MMP_RS07530 0.40 -1.6 1,426,743 - MMP_RS07530 0.71 -1.8 1,426,744 + MMP_RS07530 0.71 -0.8 1,426,886 - -1.8 1,426,947 - -2.4 1,427,029 - MMP_RS07535 0.16 -2.1 1,427,053 + MMP_RS07535 0.19 -2.4 1,427,228 - MMP_RS07535 0.42 -1.8 1,427,229 + MMP_RS07535 0.43 -1.6 1,427,256 + MMP_RS07535 0.46 -1.0 1,427,380 + MMP_RS07535 0.62 -1.1 1,427,381 - MMP_RS07535 0.63 -5.2 1,427,381 - MMP_RS07535 0.63 -1.7 1,427,382 + MMP_RS07535 0.63 -2.2
Or see this region's nucleotide sequence