Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_15645 and IAI47_15650 overlap by 4 nucleotides IAI47_15650 and IAI47_15655 are separated by 83 nucleotides IAI47_15655 and IAI47_15660 are separated by 15 nucleotides
IAI47_15645: IAI47_15645 - polynucleotide adenylyltransferase PcnB, at 3,299,813 to 3,301,252
_15645
IAI47_15650: IAI47_15650 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 3,301,249 to 3,301,734
_15650
IAI47_15655: IAI47_15655 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 3,301,818 to 3,302,612
_15655
IAI47_15660: IAI47_15660 - pantoate--beta-alanine ligase, at 3,302,628 to 3,303,482
_15660
Position (kb)
3301
3302 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3300.286 kb on + strand, within IAI47_15645 at 3300.289 kb on + strand, within IAI47_15645 at 3300.301 kb on + strand, within IAI47_15645 at 3300.347 kb on + strand, within IAI47_15645 at 3300.347 kb on + strand, within IAI47_15645 at 3300.347 kb on + strand, within IAI47_15645 at 3300.347 kb on + strand, within IAI47_15645 at 3300.347 kb on + strand, within IAI47_15645 at 3300.347 kb on + strand, within IAI47_15645 at 3300.350 kb on + strand, within IAI47_15645 at 3300.350 kb on + strand, within IAI47_15645 at 3300.350 kb on + strand, within IAI47_15645 at 3300.350 kb on + strand, within IAI47_15645 at 3300.380 kb on + strand, within IAI47_15645 at 3300.380 kb on + strand, within IAI47_15645 at 3300.380 kb on + strand, within IAI47_15645 at 3300.502 kb on + strand, within IAI47_15645 at 3300.502 kb on + strand, within IAI47_15645 at 3300.508 kb on + strand, within IAI47_15645 at 3300.508 kb on + strand, within IAI47_15645 at 3300.508 kb on + strand, within IAI47_15645 at 3300.508 kb on + strand, within IAI47_15645 at 3300.508 kb on + strand, within IAI47_15645 at 3300.555 kb on + strand, within IAI47_15645 at 3300.555 kb on + strand, within IAI47_15645 at 3300.620 kb on + strand, within IAI47_15645 at 3300.629 kb on + strand, within IAI47_15645 at 3300.629 kb on + strand, within IAI47_15645 at 3300.629 kb on + strand, within IAI47_15645 at 3300.635 kb on - strand, within IAI47_15645 at 3300.647 kb on + strand, within IAI47_15645 at 3300.647 kb on + strand, within IAI47_15645 at 3300.769 kb on + strand, within IAI47_15645 at 3300.769 kb on + strand, within IAI47_15645 at 3300.863 kb on + strand, within IAI47_15645 at 3300.895 kb on + strand, within IAI47_15645 at 3300.937 kb on + strand, within IAI47_15645 at 3300.937 kb on + strand, within IAI47_15645 at 3300.937 kb on + strand, within IAI47_15645 at 3300.940 kb on + strand, within IAI47_15645 at 3300.940 kb on + strand, within IAI47_15645 at 3300.949 kb on + strand, within IAI47_15645 at 3300.975 kb on + strand, within IAI47_15645 at 3300.975 kb on + strand, within IAI47_15645 at 3300.975 kb on + strand, within IAI47_15645 at 3300.975 kb on + strand, within IAI47_15645 at 3300.975 kb on + strand, within IAI47_15645 at 3301.043 kb on + strand, within IAI47_15645 at 3301.043 kb on + strand, within IAI47_15645 at 3301.043 kb on + strand, within IAI47_15645 at 3301.078 kb on + strand, within IAI47_15645 at 3301.078 kb on + strand, within IAI47_15645 at 3301.078 kb on + strand, within IAI47_15645 at 3301.159 kb on + strand at 3301.189 kb on + strand at 3301.189 kb on + strand at 3301.773 kb on + strand at 3301.781 kb on - strand at 3301.781 kb on - strand at 3301.890 kb on + strand at 3301.890 kb on + strand at 3301.928 kb on + strand, within IAI47_15655 at 3301.928 kb on + strand, within IAI47_15655 at 3301.928 kb on + strand, within IAI47_15655 at 3301.928 kb on + strand, within IAI47_15655 at 3301.929 kb on - strand, within IAI47_15655 at 3301.929 kb on - strand, within IAI47_15655 at 3301.929 kb on - strand, within IAI47_15655 at 3301.929 kb on - strand, within IAI47_15655 at 3302.016 kb on + strand, within IAI47_15655 at 3302.016 kb on + strand, within IAI47_15655 at 3302.016 kb on + strand, within IAI47_15655 at 3302.017 kb on - strand, within IAI47_15655 at 3302.017 kb on - strand, within IAI47_15655 at 3302.033 kb on - strand, within IAI47_15655 at 3302.078 kb on + strand, within IAI47_15655 at 3302.078 kb on + strand, within IAI47_15655 at 3302.078 kb on + strand, within IAI47_15655 at 3302.078 kb on + strand, within IAI47_15655 at 3302.078 kb on + strand, within IAI47_15655 at 3302.078 kb on + strand, within IAI47_15655 at 3302.078 kb on + strand, within IAI47_15655 at 3302.078 kb on + strand, within IAI47_15655 at 3302.079 kb on - strand, within IAI47_15655 at 3302.079 kb on - strand, within IAI47_15655 at 3302.085 kb on + strand, within IAI47_15655 at 3302.085 kb on + strand, within IAI47_15655 at 3302.085 kb on + strand, within IAI47_15655 at 3302.085 kb on + strand, within IAI47_15655 at 3302.085 kb on + strand, within IAI47_15655 at 3302.085 kb on + strand, within IAI47_15655 at 3302.085 kb on + strand, within IAI47_15655 at 3302.086 kb on - strand, within IAI47_15655 at 3302.086 kb on - strand, within IAI47_15655 at 3302.086 kb on - strand, within IAI47_15655 at 3302.086 kb on - strand, within IAI47_15655 at 3302.144 kb on + strand, within IAI47_15655 at 3302.145 kb on - strand, within IAI47_15655 at 3302.145 kb on - strand, within IAI47_15655 at 3302.145 kb on - strand, within IAI47_15655 at 3302.253 kb on - strand, within IAI47_15655 at 3302.265 kb on + strand, within IAI47_15655 at 3302.267 kb on + strand, within IAI47_15655 at 3302.267 kb on + strand, within IAI47_15655 at 3302.267 kb on + strand, within IAI47_15655 at 3302.268 kb on - strand, within IAI47_15655 at 3302.268 kb on - strand, within IAI47_15655 at 3302.268 kb on - strand, within IAI47_15655 at 3302.272 kb on + strand, within IAI47_15655 at 3302.273 kb on - strand, within IAI47_15655 at 3302.273 kb on - strand, within IAI47_15655 at 3302.353 kb on + strand, within IAI47_15655 at 3302.353 kb on + strand, within IAI47_15655 at 3302.354 kb on - strand, within IAI47_15655 at 3302.391 kb on - strand, within IAI47_15655 at 3302.407 kb on + strand, within IAI47_15655 at 3302.556 kb on + strand at 3302.556 kb on + strand at 3302.556 kb on + strand at 3302.556 kb on + strand at 3302.557 kb on - strand at 3302.557 kb on - strand at 3302.557 kb on - strand at 3302.557 kb on - strand at 3302.557 kb on - strand at 3302.557 kb on - strand at 3302.557 kb on - strand at 3302.557 kb on - strand at 3302.557 kb on - strand at 3302.559 kb on - strand at 3302.579 kb on + strand at 3302.579 kb on + strand at 3302.580 kb on - strand at 3302.580 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 3,300,286 + IAI47_15645 0.33 -1.9 3,300,289 + IAI47_15645 0.33 -0.7 3,300,301 + IAI47_15645 0.34 -3.5 3,300,347 + IAI47_15645 0.37 -1.1 3,300,347 + IAI47_15645 0.37 -1.7 3,300,347 + IAI47_15645 0.37 -3.1 3,300,347 + IAI47_15645 0.37 -1.9 3,300,347 + IAI47_15645 0.37 -0.7 3,300,347 + IAI47_15645 0.37 -0.1 3,300,350 + IAI47_15645 0.37 -2.1 3,300,350 + IAI47_15645 0.37 -1.7 3,300,350 + IAI47_15645 0.37 -0.7 3,300,350 + IAI47_15645 0.37 -1.7 3,300,380 + IAI47_15645 0.39 -1.1 3,300,380 + IAI47_15645 0.39 -0.7 3,300,380 + IAI47_15645 0.39 +1.5 3,300,502 + IAI47_15645 0.48 -2.6 3,300,502 + IAI47_15645 0.48 -0.1 3,300,508 + IAI47_15645 0.48 -2.1 3,300,508 + IAI47_15645 0.48 +0.3 3,300,508 + IAI47_15645 0.48 -3.5 3,300,508 + IAI47_15645 0.48 -1.1 3,300,508 + IAI47_15645 0.48 +0.9 3,300,555 + IAI47_15645 0.52 +0.1 3,300,555 + IAI47_15645 0.52 -0.7 3,300,620 + IAI47_15645 0.56 -0.7 3,300,629 + IAI47_15645 0.57 -1.1 3,300,629 + IAI47_15645 0.57 -0.7 3,300,629 + IAI47_15645 0.57 -0.7 3,300,635 - IAI47_15645 0.57 -0.1 3,300,647 + IAI47_15645 0.58 -3.0 3,300,647 + IAI47_15645 0.58 -1.5 3,300,769 + IAI47_15645 0.66 -1.5 3,300,769 + IAI47_15645 0.66 -2.3 3,300,863 + IAI47_15645 0.73 -2.7 3,300,895 + IAI47_15645 0.75 -2.3 3,300,937 + IAI47_15645 0.78 -1.7 3,300,937 + IAI47_15645 0.78 -2.6 3,300,937 + IAI47_15645 0.78 -1.9 3,300,940 + IAI47_15645 0.78 -3.0 3,300,940 + IAI47_15645 0.78 -2.6 3,300,949 + IAI47_15645 0.79 -0.7 3,300,975 + IAI47_15645 0.81 -1.1 3,300,975 + IAI47_15645 0.81 -3.8 3,300,975 + IAI47_15645 0.81 +0.9 3,300,975 + IAI47_15645 0.81 -2.3 3,300,975 + IAI47_15645 0.81 -2.1 3,301,043 + IAI47_15645 0.85 -1.1 3,301,043 + IAI47_15645 0.85 -1.7 3,301,043 + IAI47_15645 0.85 -0.1 3,301,078 + IAI47_15645 0.88 +0.4 3,301,078 + IAI47_15645 0.88 -1.5 3,301,078 + IAI47_15645 0.88 -0.2 3,301,159 + +0.9 3,301,189 + -1.7 3,301,189 + -0.7 3,301,773 + +0.2 3,301,781 - -0.7 3,301,781 - +0.4 3,301,890 + -0.7 3,301,890 + -1.3 3,301,928 + IAI47_15655 0.14 -0.6 3,301,928 + IAI47_15655 0.14 -1.5 3,301,928 + IAI47_15655 0.14 -0.9 3,301,928 + IAI47_15655 0.14 +0.7 3,301,929 - IAI47_15655 0.14 -1.9 3,301,929 - IAI47_15655 0.14 +0.6 3,301,929 - IAI47_15655 0.14 -0.0 3,301,929 - IAI47_15655 0.14 -1.2 3,302,016 + IAI47_15655 0.25 +0.9 3,302,016 + IAI47_15655 0.25 +0.3 3,302,016 + IAI47_15655 0.25 +0.7 3,302,017 - IAI47_15655 0.25 +0.6 3,302,017 - IAI47_15655 0.25 -0.7 3,302,033 - IAI47_15655 0.27 -0.2 3,302,078 + IAI47_15655 0.33 +0.6 3,302,078 + IAI47_15655 0.33 -0.3 3,302,078 + IAI47_15655 0.33 +0.2 3,302,078 + IAI47_15655 0.33 -1.0 3,302,078 + IAI47_15655 0.33 -0.3 3,302,078 + IAI47_15655 0.33 -0.0 3,302,078 + IAI47_15655 0.33 -0.1 3,302,078 + IAI47_15655 0.33 +1.0 3,302,079 - IAI47_15655 0.33 +0.5 3,302,079 - IAI47_15655 0.33 -0.1 3,302,085 + IAI47_15655 0.34 -0.5 3,302,085 + IAI47_15655 0.34 -0.5 3,302,085 + IAI47_15655 0.34 +0.7 3,302,085 + IAI47_15655 0.34 -1.1 3,302,085 + IAI47_15655 0.34 -0.6 3,302,085 + IAI47_15655 0.34 +0.4 3,302,085 + IAI47_15655 0.34 -0.5 3,302,086 - IAI47_15655 0.34 -0.7 3,302,086 - IAI47_15655 0.34 +0.2 3,302,086 - IAI47_15655 0.34 -1.3 3,302,086 - IAI47_15655 0.34 -2.7 3,302,144 + IAI47_15655 0.41 +0.9 3,302,145 - IAI47_15655 0.41 +0.9 3,302,145 - IAI47_15655 0.41 -0.0 3,302,145 - IAI47_15655 0.41 -2.6 3,302,253 - IAI47_15655 0.55 -0.5 3,302,265 + IAI47_15655 0.56 +0.6 3,302,267 + IAI47_15655 0.56 -0.6 3,302,267 + IAI47_15655 0.56 +1.9 3,302,267 + IAI47_15655 0.56 +0.1 3,302,268 - IAI47_15655 0.57 -0.0 3,302,268 - IAI47_15655 0.57 -0.7 3,302,268 - IAI47_15655 0.57 -0.1 3,302,272 + IAI47_15655 0.57 -0.1 3,302,273 - IAI47_15655 0.57 -0.5 3,302,273 - IAI47_15655 0.57 -1.1 3,302,353 + IAI47_15655 0.67 +0.1 3,302,353 + IAI47_15655 0.67 -0.5 3,302,354 - IAI47_15655 0.67 +0.9 3,302,391 - IAI47_15655 0.72 +1.1 3,302,407 + IAI47_15655 0.74 +1.2 3,302,556 + +0.4 3,302,556 + +4.0 3,302,556 + +0.2 3,302,556 + -0.1 3,302,557 - -0.8 3,302,557 - +0.5 3,302,557 - +0.9 3,302,557 - +0.5 3,302,557 - -0.1 3,302,557 - -0.7 3,302,557 - -0.7 3,302,557 - -0.1 3,302,557 - -1.9 3,302,559 - +0.1 3,302,579 + -0.5 3,302,579 + +0.3 3,302,580 - -0.6 3,302,580 - -2.5
Or see this region's nucleotide sequence