Experiment: Ying_AminoAcid20 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_00720 and IAI47_00725 are separated by 252 nucleotides IAI47_00725 and IAI47_00730 are separated by 19 nucleotides IAI47_00730 and IAI47_00735 are separated by 38 nucleotides IAI47_00735 and IAI47_00740 overlap by 1 nucleotides
IAI47_00720: IAI47_00720 - murein hydrolase activator EnvC, at 155,232 to 156,533
_00720
IAI47_00725: IAI47_00725 - rhodanese-like domain-containing protein, at 156,786 to 157,217
_00725
IAI47_00730: IAI47_00730 - glutaredoxin 3, at 157,237 to 157,491
_00730
IAI47_00735: IAI47_00735 - protein-export chaperone SecB, at 157,530 to 158,000
_00735
IAI47_00740: IAI47_00740 - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 158,000 to 159,016
_00740
Position (kb) 157
158
159 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 156.533 kb on + strand at 156.533 kb on + strand at 156.533 kb on + strand at 156.533 kb on + strand at 156.533 kb on + strand at 156.533 kb on + strand at 156.533 kb on + strand at 156.534 kb on - strand at 156.534 kb on - strand at 156.534 kb on - strand at 156.534 kb on - strand at 156.545 kb on + strand at 156.545 kb on + strand at 156.545 kb on + strand at 156.546 kb on - strand at 156.546 kb on - strand at 156.546 kb on - strand at 156.567 kb on + strand at 156.602 kb on + strand at 156.602 kb on + strand at 156.602 kb on + strand at 156.602 kb on + strand at 156.602 kb on + strand at 156.602 kb on + strand at 156.602 kb on + strand at 156.603 kb on - strand at 156.603 kb on - strand at 156.603 kb on - strand at 156.603 kb on - strand at 156.608 kb on + strand at 156.608 kb on + strand at 156.609 kb on - strand at 156.609 kb on - strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.629 kb on + strand at 156.629 kb on + strand at 156.629 kb on + strand at 156.629 kb on + strand at 156.629 kb on + strand at 156.630 kb on - strand at 156.630 kb on - strand at 156.630 kb on - strand at 156.630 kb on - strand at 156.630 kb on - strand at 156.630 kb on - strand at 156.637 kb on - strand at 156.651 kb on - strand at 156.689 kb on + strand at 156.689 kb on + strand at 156.702 kb on + strand at 156.702 kb on + strand at 156.702 kb on + strand at 156.703 kb on - strand at 156.703 kb on - strand at 156.703 kb on - strand at 156.711 kb on + strand at 156.711 kb on + strand at 156.711 kb on + strand at 156.711 kb on + strand at 156.711 kb on + strand at 156.711 kb on + strand at 156.711 kb on + strand at 156.711 kb on + strand at 156.712 kb on - strand at 156.712 kb on - strand at 156.712 kb on - strand at 156.712 kb on - strand at 156.712 kb on - strand at 156.712 kb on - strand at 156.731 kb on + strand at 156.731 kb on + strand at 156.731 kb on + strand at 156.731 kb on + strand at 156.731 kb on + strand at 156.732 kb on - strand at 156.732 kb on - strand at 156.733 kb on + strand at 156.751 kb on + strand at 156.751 kb on + strand at 156.756 kb on + strand at 156.756 kb on + strand at 156.756 kb on + strand at 156.756 kb on + strand at 156.756 kb on + strand at 156.756 kb on + strand at 156.756 kb on + strand at 156.756 kb on + strand at 156.759 kb on + strand at 156.759 kb on + strand at 156.797 kb on + strand at 156.890 kb on + strand, within IAI47_00725 at 156.890 kb on + strand, within IAI47_00725 at 156.891 kb on - strand, within IAI47_00725 at 156.949 kb on + strand, within IAI47_00725 at 156.955 kb on + strand, within IAI47_00725 at 156.955 kb on + strand, within IAI47_00725 at 156.955 kb on + strand, within IAI47_00725 at 156.955 kb on + strand, within IAI47_00725 at 157.028 kb on + strand, within IAI47_00725 at 157.075 kb on + strand, within IAI47_00725 at 157.075 kb on + strand, within IAI47_00725 at 157.075 kb on + strand, within IAI47_00725 at 157.075 kb on + strand, within IAI47_00725 at 157.075 kb on + strand, within IAI47_00725 at 157.075 kb on + strand, within IAI47_00725 at 157.084 kb on + strand, within IAI47_00725 at 157.084 kb on + strand, within IAI47_00725 at 157.084 kb on + strand, within IAI47_00725 at 157.084 kb on + strand, within IAI47_00725 at 157.169 kb on + strand, within IAI47_00725 at 157.169 kb on + strand, within IAI47_00725 at 157.169 kb on + strand, within IAI47_00725 at 157.201 kb on + strand at 157.201 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.221 kb on + strand at 157.221 kb on + strand at 157.221 kb on + strand at 157.221 kb on + strand at 157.255 kb on + strand at 157.255 kb on + strand at 157.255 kb on + strand at 157.255 kb on + strand at 157.255 kb on + strand at 157.255 kb on + strand at 157.255 kb on + strand at 157.257 kb on + strand at 157.257 kb on + strand at 157.257 kb on + strand at 157.257 kb on + strand at 157.276 kb on + strand, within IAI47_00730 at 157.276 kb on + strand, within IAI47_00730 at 157.316 kb on + strand, within IAI47_00730 at 157.335 kb on + strand, within IAI47_00730 at 157.386 kb on + strand, within IAI47_00730 at 157.442 kb on + strand, within IAI47_00730 at 157.442 kb on + strand, within IAI47_00730 at 157.442 kb on + strand, within IAI47_00730 at 157.469 kb on + strand at 157.469 kb on + strand at 157.469 kb on + strand at 157.478 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.541 kb on + strand at 157.541 kb on + strand at 157.541 kb on + strand at 157.541 kb on + strand at 157.649 kb on + strand, within IAI47_00735 at 157.695 kb on + strand, within IAI47_00735 at 157.720 kb on + strand, within IAI47_00735 at 157.830 kb on + strand, within IAI47_00735 at 157.892 kb on + strand, within IAI47_00735
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 2 remove 156,533 + +0.2 156,533 + -0.3 156,533 + -0.4 156,533 + +0.4 156,533 + -1.3 156,533 + -1.2 156,533 + -1.2 156,534 - +0.3 156,534 - -2.8 156,534 - +0.0 156,534 - +1.8 156,545 + -0.3 156,545 + -0.8 156,545 + -0.8 156,546 - +3.4 156,546 - +0.0 156,546 - +0.5 156,567 + -1.1 156,602 + -0.8 156,602 + -1.2 156,602 + +0.1 156,602 + -0.4 156,602 + -0.0 156,602 + +0.1 156,602 + +0.8 156,603 - -1.0 156,603 - -0.8 156,603 - -0.7 156,603 - +2.3 156,608 + -0.9 156,608 + +0.2 156,609 - -0.2 156,609 - -0.9 156,624 + +0.0 156,624 + +0.8 156,624 + +0.4 156,624 + -0.2 156,624 + -0.2 156,624 + +1.2 156,624 + -1.2 156,624 + +0.1 156,624 + +0.3 156,624 + -1.6 156,624 + +1.6 156,624 + -0.8 156,624 + +0.8 156,624 + -0.3 156,624 + -0.3 156,624 + +0.2 156,625 - +1.6 156,625 - -1.0 156,625 - +0.2 156,625 - +0.4 156,625 - -0.2 156,625 - -0.8 156,625 - +0.0 156,625 - +0.2 156,625 - -0.2 156,625 - -0.1 156,625 - +1.3 156,625 - -0.3 156,625 - -0.2 156,625 - -1.2 156,625 - +1.0 156,629 + +1.0 156,629 + -0.0 156,629 + -1.0 156,629 + -0.0 156,629 + +1.4 156,630 - -0.3 156,630 - -2.2 156,630 - +0.5 156,630 - +1.1 156,630 - -1.9 156,630 - +0.6 156,637 - +0.8 156,651 - +0.7 156,689 + +0.6 156,689 + +0.1 156,702 + +0.5 156,702 + +0.5 156,702 + -0.1 156,703 - +0.7 156,703 - +0.1 156,703 - -1.1 156,711 + -2.0 156,711 + +0.3 156,711 + -0.6 156,711 + +0.1 156,711 + -0.9 156,711 + -0.6 156,711 + -1.8 156,711 + -0.2 156,712 - +0.3 156,712 - +0.3 156,712 - +1.0 156,712 - -0.1 156,712 - -0.9 156,712 - -2.2 156,731 + -0.9 156,731 + -0.5 156,731 + -1.2 156,731 + -1.3 156,731 + +1.3 156,732 - +1.4 156,732 - -2.5 156,733 + -1.2 156,751 + +0.2 156,751 + +0.3 156,756 + -0.3 156,756 + -0.2 156,756 + -0.2 156,756 + +0.8 156,756 + +0.4 156,756 + -2.0 156,756 + +0.2 156,756 + +1.3 156,759 + -0.8 156,759 + +0.8 156,797 + +0.2 156,890 + IAI47_00725 0.24 +0.2 156,890 + IAI47_00725 0.24 -0.8 156,891 - IAI47_00725 0.24 +1.4 156,949 + IAI47_00725 0.38 +1.8 156,955 + IAI47_00725 0.39 +0.4 156,955 + IAI47_00725 0.39 +0.6 156,955 + IAI47_00725 0.39 -1.0 156,955 + IAI47_00725 0.39 +1.4 157,028 + IAI47_00725 0.56 -0.1 157,075 + IAI47_00725 0.67 -1.0 157,075 + IAI47_00725 0.67 +1.8 157,075 + IAI47_00725 0.67 -0.6 157,075 + IAI47_00725 0.67 -0.1 157,075 + IAI47_00725 0.67 -0.6 157,075 + IAI47_00725 0.67 -0.3 157,084 + IAI47_00725 0.69 -0.8 157,084 + IAI47_00725 0.69 -1.0 157,084 + IAI47_00725 0.69 -1.8 157,084 + IAI47_00725 0.69 +0.5 157,169 + IAI47_00725 0.89 -2.5 157,169 + IAI47_00725 0.89 -0.3 157,169 + IAI47_00725 0.89 +0.3 157,201 + -1.9 157,201 + +0.2 157,215 + -0.3 157,215 + +0.4 157,215 + +0.7 157,215 + +0.5 157,215 + -0.5 157,215 + -1.6 157,215 + -1.1 157,215 + +0.8 157,215 + -1.5 157,215 + -0.6 157,215 + +0.2 157,215 + -0.8 157,215 + +0.1 157,221 + -1.5 157,221 + -0.6 157,221 + -0.6 157,221 + +0.8 157,255 + -1.2 157,255 + +0.3 157,255 + -0.0 157,255 + -0.2 157,255 + -1.0 157,255 + +1.8 157,255 + +1.4 157,257 + -1.3 157,257 + +0.5 157,257 + -0.8 157,257 + -0.8 157,276 + IAI47_00730 0.15 -0.8 157,276 + IAI47_00730 0.15 +0.2 157,316 + IAI47_00730 0.31 -0.4 157,335 + IAI47_00730 0.38 -0.8 157,386 + IAI47_00730 0.58 -1.8 157,442 + IAI47_00730 0.80 +1.1 157,442 + IAI47_00730 0.80 -0.8 157,442 + IAI47_00730 0.80 +0.5 157,469 + -0.4 157,469 + +2.8 157,469 + +0.1 157,478 + +0.8 157,512 + -1.2 157,512 + +0.9 157,512 + -0.5 157,512 + -0.5 157,512 + -0.7 157,512 + +0.4 157,512 + -0.4 157,512 + -0.0 157,512 + -0.3 157,512 + -0.6 157,512 + -0.5 157,512 + +1.1 157,512 + +0.4 157,516 + -1.2 157,516 + +0.8 157,516 + +1.4 157,516 + +0.2 157,516 + -0.3 157,516 + -2.6 157,516 + -1.3 157,516 + +0.1 157,516 + -0.9 157,516 + -0.0 157,516 + -1.8 157,516 + +0.8 157,516 + -0.3 157,516 + +0.6 157,516 + -0.2 157,516 + -0.4 157,516 + -0.4 157,516 + -0.4 157,541 + -1.5 157,541 + -2.3 157,541 + -1.5 157,541 + -0.5 157,649 + IAI47_00735 0.25 -1.8 157,695 + IAI47_00735 0.35 -1.2 157,720 + IAI47_00735 0.40 -1.5 157,830 + IAI47_00735 0.64 -0.2 157,892 + IAI47_00735 0.77 -0.8
Or see this region's nucleotide sequence