Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00156 and MPMX20_00157 are separated by 3 nucleotides MPMX20_00157 and MPMX20_00158 are separated by 4 nucleotides MPMX20_00158 and MPMX20_00159 are separated by 180 nucleotides
MPMX20_00156: MPMX20_00156 - DNA-binding transcriptional repressor YiaJ, at 159,794 to 160,612
_00156
MPMX20_00157: MPMX20_00157 - Iron-sulfur protein, at 160,616 to 161,071
_00157
MPMX20_00158: MPMX20_00158 - Valine--pyruvate aminotransferase, at 161,076 to 162,332
_00158
MPMX20_00159: MPMX20_00159 - Periplasmic alpha-amylase, at 162,513 to 164,543
_00159
Position (kb) 161
162
163 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 160.092 kb on - strand, within MPMX20_00156 at 160.100 kb on - strand, within MPMX20_00156 at 160.107 kb on - strand, within MPMX20_00156 at 160.119 kb on + strand, within MPMX20_00156 at 160.130 kb on + strand, within MPMX20_00156 at 160.165 kb on - strand, within MPMX20_00156 at 160.186 kb on + strand, within MPMX20_00156 at 160.194 kb on + strand, within MPMX20_00156 at 160.194 kb on - strand, within MPMX20_00156 at 160.194 kb on - strand, within MPMX20_00156 at 160.231 kb on - strand, within MPMX20_00156 at 160.245 kb on - strand, within MPMX20_00156 at 160.279 kb on - strand, within MPMX20_00156 at 160.293 kb on - strand, within MPMX20_00156 at 160.314 kb on + strand, within MPMX20_00156 at 160.362 kb on + strand, within MPMX20_00156 at 160.407 kb on - strand, within MPMX20_00156 at 160.407 kb on - strand, within MPMX20_00156 at 160.446 kb on - strand, within MPMX20_00156 at 160.466 kb on + strand, within MPMX20_00156 at 160.543 kb on + strand at 160.590 kb on + strand at 160.590 kb on + strand at 160.598 kb on - strand at 160.598 kb on - strand at 160.598 kb on - strand at 160.665 kb on + strand, within MPMX20_00157 at 160.688 kb on - strand, within MPMX20_00157 at 160.736 kb on - strand, within MPMX20_00157 at 160.756 kb on + strand, within MPMX20_00157 at 160.763 kb on + strand, within MPMX20_00157 at 160.771 kb on - strand, within MPMX20_00157 at 160.823 kb on + strand, within MPMX20_00157 at 160.975 kb on - strand, within MPMX20_00157 at 160.987 kb on - strand, within MPMX20_00157 at 160.989 kb on + strand, within MPMX20_00157 at 160.989 kb on + strand, within MPMX20_00157 at 160.989 kb on + strand, within MPMX20_00157 at 160.989 kb on + strand, within MPMX20_00157 at 160.989 kb on + strand, within MPMX20_00157 at 160.997 kb on - strand, within MPMX20_00157 at 160.997 kb on - strand, within MPMX20_00157 at 161.020 kb on - strand, within MPMX20_00157 at 161.030 kb on - strand at 161.030 kb on - strand at 161.030 kb on - strand at 161.030 kb on - strand at 161.092 kb on + strand at 161.171 kb on - strand at 161.191 kb on + strand at 161.202 kb on + strand, within MPMX20_00158 at 161.210 kb on - strand, within MPMX20_00158 at 161.210 kb on - strand, within MPMX20_00158 at 161.220 kb on - strand, within MPMX20_00158 at 161.411 kb on + strand, within MPMX20_00158 at 161.449 kb on - strand, within MPMX20_00158 at 161.492 kb on + strand, within MPMX20_00158 at 161.500 kb on - strand, within MPMX20_00158 at 161.594 kb on + strand, within MPMX20_00158 at 161.594 kb on + strand, within MPMX20_00158 at 161.595 kb on + strand, within MPMX20_00158 at 161.668 kb on + strand, within MPMX20_00158 at 161.668 kb on + strand, within MPMX20_00158 at 161.668 kb on + strand, within MPMX20_00158 at 161.668 kb on + strand, within MPMX20_00158 at 161.676 kb on - strand, within MPMX20_00158 at 161.676 kb on - strand, within MPMX20_00158 at 161.676 kb on - strand, within MPMX20_00158 at 161.743 kb on + strand, within MPMX20_00158 at 161.784 kb on + strand, within MPMX20_00158 at 161.792 kb on - strand, within MPMX20_00158 at 161.792 kb on - strand, within MPMX20_00158 at 161.792 kb on - strand, within MPMX20_00158 at 161.816 kb on + strand, within MPMX20_00158 at 161.843 kb on + strand, within MPMX20_00158 at 161.865 kb on - strand, within MPMX20_00158 at 161.866 kb on - strand, within MPMX20_00158 at 161.888 kb on - strand, within MPMX20_00158 at 161.919 kb on + strand, within MPMX20_00158 at 161.970 kb on + strand, within MPMX20_00158 at 162.060 kb on - strand, within MPMX20_00158 at 162.065 kb on - strand, within MPMX20_00158 at 162.074 kb on + strand, within MPMX20_00158 at 162.082 kb on - strand, within MPMX20_00158 at 162.087 kb on - strand, within MPMX20_00158 at 162.103 kb on + strand, within MPMX20_00158 at 162.103 kb on + strand, within MPMX20_00158 at 162.106 kb on - strand, within MPMX20_00158 at 162.239 kb on + strand at 162.259 kb on + strand at 162.267 kb on - strand at 162.282 kb on + strand at 162.282 kb on + strand at 162.286 kb on - strand at 162.327 kb on + strand at 162.337 kb on + strand at 162.371 kb on + strand at 162.371 kb on + strand at 162.379 kb on - strand at 162.385 kb on - strand at 162.385 kb on - strand at 162.385 kb on - strand at 162.385 kb on - strand at 162.392 kb on + strand at 162.487 kb on + strand at 162.496 kb on - strand at 162.526 kb on + strand at 162.535 kb on + strand at 162.535 kb on + strand at 162.535 kb on + strand at 162.535 kb on + strand at 162.535 kb on + strand at 162.535 kb on + strand at 162.535 kb on + strand at 162.543 kb on - strand at 162.543 kb on - strand at 162.543 kb on - strand at 162.543 kb on - strand at 162.550 kb on + strand at 162.570 kb on + strand at 162.596 kb on + strand at 162.631 kb on + strand at 162.642 kb on + strand at 162.667 kb on + strand at 162.667 kb on + strand at 162.667 kb on + strand at 162.697 kb on - strand at 162.699 kb on + strand at 162.699 kb on + strand at 162.699 kb on + strand at 162.699 kb on + strand at 162.707 kb on - strand at 162.707 kb on - strand at 162.707 kb on - strand at 162.720 kb on + strand, within MPMX20_00159 at 162.728 kb on + strand, within MPMX20_00159 at 162.733 kb on + strand, within MPMX20_00159 at 162.736 kb on - strand, within MPMX20_00159 at 162.736 kb on - strand, within MPMX20_00159 at 162.743 kb on + strand, within MPMX20_00159 at 162.802 kb on - strand, within MPMX20_00159 at 162.807 kb on - strand, within MPMX20_00159 at 162.817 kb on - strand, within MPMX20_00159 at 162.837 kb on + strand, within MPMX20_00159 at 162.865 kb on - strand, within MPMX20_00159 at 162.875 kb on - strand, within MPMX20_00159 at 162.875 kb on - strand, within MPMX20_00159 at 162.917 kb on + strand, within MPMX20_00159 at 162.934 kb on - strand, within MPMX20_00159 at 162.983 kb on + strand, within MPMX20_00159 at 162.983 kb on + strand, within MPMX20_00159 at 163.055 kb on + strand, within MPMX20_00159 at 163.167 kb on - strand, within MPMX20_00159 at 163.191 kb on + strand, within MPMX20_00159 at 163.201 kb on - strand, within MPMX20_00159 at 163.231 kb on - strand, within MPMX20_00159 at 163.270 kb on + strand, within MPMX20_00159 at 163.271 kb on - strand, within MPMX20_00159 at 163.271 kb on - strand, within MPMX20_00159 at 163.278 kb on - strand, within MPMX20_00159 at 163.278 kb on - strand, within MPMX20_00159 at 163.285 kb on + strand, within MPMX20_00159
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 160,092 - MPMX20_00156 0.36 +0.8 160,100 - MPMX20_00156 0.37 +0.0 160,107 - MPMX20_00156 0.38 +0.5 160,119 + MPMX20_00156 0.40 +1.6 160,130 + MPMX20_00156 0.41 +0.9 160,165 - MPMX20_00156 0.45 -0.1 160,186 + MPMX20_00156 0.48 +0.5 160,194 + MPMX20_00156 0.49 +0.3 160,194 - MPMX20_00156 0.49 -0.8 160,194 - MPMX20_00156 0.49 +0.3 160,231 - MPMX20_00156 0.53 -0.2 160,245 - MPMX20_00156 0.55 +0.1 160,279 - MPMX20_00156 0.59 +2.2 160,293 - MPMX20_00156 0.61 +0.6 160,314 + MPMX20_00156 0.63 -1.4 160,362 + MPMX20_00156 0.69 +0.5 160,407 - MPMX20_00156 0.75 -0.4 160,407 - MPMX20_00156 0.75 +0.4 160,446 - MPMX20_00156 0.80 +0.3 160,466 + MPMX20_00156 0.82 -0.3 160,543 + -0.9 160,590 + +1.7 160,590 + -1.4 160,598 - +0.8 160,598 - -1.8 160,598 - -0.7 160,665 + MPMX20_00157 0.11 +0.2 160,688 - MPMX20_00157 0.16 +0.1 160,736 - MPMX20_00157 0.26 +0.4 160,756 + MPMX20_00157 0.31 -1.3 160,763 + MPMX20_00157 0.32 -2.4 160,771 - MPMX20_00157 0.34 +0.8 160,823 + MPMX20_00157 0.45 -2.2 160,975 - MPMX20_00157 0.79 +0.1 160,987 - MPMX20_00157 0.81 -0.3 160,989 + MPMX20_00157 0.82 +0.1 160,989 + MPMX20_00157 0.82 -0.2 160,989 + MPMX20_00157 0.82 +1.0 160,989 + MPMX20_00157 0.82 -0.1 160,989 + MPMX20_00157 0.82 +0.1 160,997 - MPMX20_00157 0.84 -0.3 160,997 - MPMX20_00157 0.84 -0.6 161,020 - MPMX20_00157 0.89 -1.8 161,030 - +0.5 161,030 - -1.2 161,030 - -0.4 161,030 - +0.1 161,092 + -1.0 161,171 - -0.5 161,191 + -0.4 161,202 + MPMX20_00158 0.10 -0.0 161,210 - MPMX20_00158 0.11 +0.9 161,210 - MPMX20_00158 0.11 +0.1 161,220 - MPMX20_00158 0.11 +0.3 161,411 + MPMX20_00158 0.27 +1.2 161,449 - MPMX20_00158 0.30 -0.2 161,492 + MPMX20_00158 0.33 -0.5 161,500 - MPMX20_00158 0.34 -0.7 161,594 + MPMX20_00158 0.41 +1.4 161,594 + MPMX20_00158 0.41 -2.2 161,595 + MPMX20_00158 0.41 -0.3 161,668 + MPMX20_00158 0.47 -0.1 161,668 + MPMX20_00158 0.47 -0.3 161,668 + MPMX20_00158 0.47 +1.2 161,668 + MPMX20_00158 0.47 -0.7 161,676 - MPMX20_00158 0.48 +1.1 161,676 - MPMX20_00158 0.48 +2.5 161,676 - MPMX20_00158 0.48 -0.4 161,743 + MPMX20_00158 0.53 -2.1 161,784 + MPMX20_00158 0.56 +0.5 161,792 - MPMX20_00158 0.57 -0.3 161,792 - MPMX20_00158 0.57 +0.5 161,792 - MPMX20_00158 0.57 -1.6 161,816 + MPMX20_00158 0.59 -1.7 161,843 + MPMX20_00158 0.61 +3.8 161,865 - MPMX20_00158 0.63 +1.0 161,866 - MPMX20_00158 0.63 -0.4 161,888 - MPMX20_00158 0.65 -2.1 161,919 + MPMX20_00158 0.67 +2.0 161,970 + MPMX20_00158 0.71 +0.5 162,060 - MPMX20_00158 0.78 -0.3 162,065 - MPMX20_00158 0.79 -0.2 162,074 + MPMX20_00158 0.79 -0.2 162,082 - MPMX20_00158 0.80 +0.1 162,087 - MPMX20_00158 0.80 +0.2 162,103 + MPMX20_00158 0.82 -1.4 162,103 + MPMX20_00158 0.82 -1.6 162,106 - MPMX20_00158 0.82 -0.4 162,239 + +0.7 162,259 + -0.5 162,267 - -0.2 162,282 + +0.4 162,282 + +0.3 162,286 - -0.6 162,327 + +0.7 162,337 + +1.5 162,371 + +0.4 162,371 + +1.4 162,379 - +0.9 162,385 - +0.1 162,385 - -1.2 162,385 - +0.2 162,385 - +0.5 162,392 + -2.6 162,487 + -0.4 162,496 - -1.3 162,526 + -0.6 162,535 + -0.3 162,535 + -0.7 162,535 + +0.1 162,535 + -1.4 162,535 + +0.7 162,535 + -0.4 162,535 + -2.1 162,543 - +1.2 162,543 - +1.4 162,543 - +1.1 162,543 - +0.2 162,550 + +0.6 162,570 + -1.9 162,596 + -0.4 162,631 + +1.2 162,642 + +0.6 162,667 + -0.5 162,667 + +2.8 162,667 + -0.4 162,697 - +0.6 162,699 + +0.0 162,699 + +1.2 162,699 + -0.5 162,699 + -1.0 162,707 - +0.4 162,707 - -1.7 162,707 - +0.8 162,720 + MPMX20_00159 0.10 +0.6 162,728 + MPMX20_00159 0.11 +1.2 162,733 + MPMX20_00159 0.11 -0.7 162,736 - MPMX20_00159 0.11 -1.4 162,736 - MPMX20_00159 0.11 +1.8 162,743 + MPMX20_00159 0.11 -1.9 162,802 - MPMX20_00159 0.14 +0.1 162,807 - MPMX20_00159 0.14 +1.4 162,817 - MPMX20_00159 0.15 +0.9 162,837 + MPMX20_00159 0.16 -0.8 162,865 - MPMX20_00159 0.17 +1.3 162,875 - MPMX20_00159 0.18 -1.5 162,875 - MPMX20_00159 0.18 +1.0 162,917 + MPMX20_00159 0.20 -1.4 162,934 - MPMX20_00159 0.21 -0.3 162,983 + MPMX20_00159 0.23 +0.3 162,983 + MPMX20_00159 0.23 +1.2 163,055 + MPMX20_00159 0.27 +0.4 163,167 - MPMX20_00159 0.32 -1.9 163,191 + MPMX20_00159 0.33 -0.9 163,201 - MPMX20_00159 0.34 +0.7 163,231 - MPMX20_00159 0.35 +0.2 163,270 + MPMX20_00159 0.37 -1.3 163,271 - MPMX20_00159 0.37 +0.4 163,271 - MPMX20_00159 0.37 +1.9 163,278 - MPMX20_00159 0.38 +2.2 163,278 - MPMX20_00159 0.38 -0.7 163,285 + MPMX20_00159 0.38 +1.8
Or see this region's nucleotide sequence