Experiment: Ammonium chloride (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS04525 and HMPREF1058_RS04520 are separated by 667 nucleotides HMPREF1058_RS04520 and HMPREF1058_RS04515 are separated by 3 nucleotides HMPREF1058_RS04515 and HMPREF1058_RS20295 are separated by 121 nucleotides HMPREF1058_RS20295 and HMPREF1058_RS04510 are separated by 20 nucleotides HMPREF1058_RS04510 and HMPREF1058_RS04505 are separated by 7 nucleotides
HMPREF1058_RS04525: HMPREF1058_RS04525 - hypothetical protein, at 11,750 to 12,043
_RS04525
HMPREF1058_RS04520: HMPREF1058_RS04520 - hypothetical protein, at 12,711 to 12,926
_RS04520
HMPREF1058_RS04515: HMPREF1058_RS04515 - hypothetical protein, at 12,930 to 13,127
_RS04515
HMPREF1058_RS20295: HMPREF1058_RS20295 - hypothetical protein, at 13,249 to 13,641
_RS20295
HMPREF1058_RS04510: HMPREF1058_RS04510 - hypothetical protein, at 13,662 to 14,102
_RS04510
HMPREF1058_RS04505: HMPREF1058_RS04505 - type I restriction enzyme HsdR N-terminal domain-containing protein, at 14,110 to 15,195
_RS04505
Position (kb) 12
13
14 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 12.000 kb on + strand, within HMPREF1058_RS04525 at 12.191 kb on + strand at 12.238 kb on + strand at 12.331 kb on + strand at 12.553 kb on + strand at 12.573 kb on + strand at 12.573 kb on + strand at 12.676 kb on + strand at 12.712 kb on + strand at 12.814 kb on + strand, within HMPREF1058_RS04520 at 12.931 kb on + strand at 12.931 kb on + strand at 13.009 kb on + strand, within HMPREF1058_RS04515 at 13.020 kb on + strand, within HMPREF1058_RS04515 at 13.455 kb on + strand, within HMPREF1058_RS20295 at 13.455 kb on + strand, within HMPREF1058_RS20295 at 13.465 kb on + strand, within HMPREF1058_RS20295 at 13.466 kb on - strand, within HMPREF1058_RS20295 at 13.646 kb on + strand at 13.651 kb on - strand at 13.674 kb on - strand at 13.674 kb on - strand at 13.746 kb on - strand, within HMPREF1058_RS04510 at 13.746 kb on - strand, within HMPREF1058_RS04510 at 13.877 kb on + strand, within HMPREF1058_RS04510 at 13.879 kb on + strand, within HMPREF1058_RS04510 at 13.879 kb on + strand, within HMPREF1058_RS04510 at 13.880 kb on - strand, within HMPREF1058_RS04510 at 13.949 kb on + strand, within HMPREF1058_RS04510 at 13.950 kb on - strand, within HMPREF1058_RS04510 at 14.006 kb on - strand, within HMPREF1058_RS04510 at 14.043 kb on + strand, within HMPREF1058_RS04510 at 14.044 kb on - strand, within HMPREF1058_RS04510 at 14.045 kb on + strand, within HMPREF1058_RS04510 at 14.053 kb on - strand, within HMPREF1058_RS04510 at 14.105 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 12,000 + HMPREF1058_RS04525 0.85 -0.2 12,191 + +2.7 12,238 + -0.3 12,331 + -1.1 12,553 + -0.2 12,573 + -0.7 12,573 + +0.6 12,676 + -1.2 12,712 + +0.1 12,814 + HMPREF1058_RS04520 0.48 -1.0 12,931 + +0.1 12,931 + +1.1 13,009 + HMPREF1058_RS04515 0.40 -0.9 13,020 + HMPREF1058_RS04515 0.45 -1.4 13,455 + HMPREF1058_RS20295 0.52 +0.3 13,455 + HMPREF1058_RS20295 0.52 +0.6 13,465 + HMPREF1058_RS20295 0.55 +2.1 13,466 - HMPREF1058_RS20295 0.55 -2.8 13,646 + -0.7 13,651 - -1.7 13,674 - -1.7 13,674 - +3.9 13,746 - HMPREF1058_RS04510 0.19 -1.6 13,746 - HMPREF1058_RS04510 0.19 -0.2 13,877 + HMPREF1058_RS04510 0.49 -1.3 13,879 + HMPREF1058_RS04510 0.49 -2.2 13,879 + HMPREF1058_RS04510 0.49 -0.3 13,880 - HMPREF1058_RS04510 0.49 +0.8 13,949 + HMPREF1058_RS04510 0.65 +1.5 13,950 - HMPREF1058_RS04510 0.65 +0.3 14,006 - HMPREF1058_RS04510 0.78 -1.1 14,043 + HMPREF1058_RS04510 0.86 +0.6 14,044 - HMPREF1058_RS04510 0.87 +0.5 14,045 + HMPREF1058_RS04510 0.87 +2.1 14,053 - HMPREF1058_RS04510 0.89 -0.1 14,105 + +1.2
Or see this region's nucleotide sequence