Experiment: Ammonium chloride (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS01495 and HMPREF1058_RS01490 are separated by 296 nucleotides HMPREF1058_RS01490 and HMPREF1058_RS01485 are separated by 90 nucleotides
HMPREF1058_RS01495: HMPREF1058_RS01495 - TonB-dependent receptor, at 109,389 to 111,692
_RS01495
HMPREF1058_RS01490: HMPREF1058_RS01490 - methylenetetrahydrofolate reductase [NAD(P)H], at 111,989 to 112,942
_RS01490
HMPREF1058_RS01485: HMPREF1058_RS01485 - ribonucleotide-diphosphate reductase subunit beta, at 113,033 to 114,082
_RS01485
Position (kb)
111
112
113 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 110.993 kb on + strand, within HMPREF1058_RS01495 at 111.097 kb on + strand, within HMPREF1058_RS01495 at 111.164 kb on + strand, within HMPREF1058_RS01495 at 111.180 kb on + strand, within HMPREF1058_RS01495 at 111.181 kb on - strand, within HMPREF1058_RS01495 at 111.203 kb on - strand, within HMPREF1058_RS01495 at 111.205 kb on - strand, within HMPREF1058_RS01495 at 111.205 kb on - strand, within HMPREF1058_RS01495 at 111.266 kb on + strand, within HMPREF1058_RS01495 at 111.356 kb on + strand, within HMPREF1058_RS01495 at 111.381 kb on + strand, within HMPREF1058_RS01495 at 111.381 kb on + strand, within HMPREF1058_RS01495 at 111.423 kb on + strand, within HMPREF1058_RS01495 at 111.426 kb on - strand, within HMPREF1058_RS01495 at 111.435 kb on - strand, within HMPREF1058_RS01495 at 111.539 kb on + strand at 111.572 kb on - strand at 111.572 kb on - strand at 111.576 kb on - strand at 111.576 kb on - strand at 111.576 kb on - strand at 111.576 kb on - strand at 111.576 kb on - strand at 111.578 kb on + strand at 111.663 kb on + strand at 111.690 kb on + strand at 111.691 kb on - strand at 111.702 kb on - strand at 111.754 kb on - strand at 111.763 kb on - strand at 111.772 kb on + strand at 111.935 kb on + strand at 111.947 kb on - strand at 111.973 kb on - strand at 112.088 kb on - strand, within HMPREF1058_RS01490 at 112.097 kb on - strand, within HMPREF1058_RS01490 at 112.152 kb on - strand, within HMPREF1058_RS01490 at 112.152 kb on - strand, within HMPREF1058_RS01490 at 112.152 kb on - strand, within HMPREF1058_RS01490 at 112.152 kb on - strand, within HMPREF1058_RS01490 at 112.179 kb on + strand, within HMPREF1058_RS01490 at 112.180 kb on - strand, within HMPREF1058_RS01490 at 112.180 kb on - strand, within HMPREF1058_RS01490 at 112.181 kb on + strand, within HMPREF1058_RS01490 at 112.182 kb on - strand, within HMPREF1058_RS01490 at 112.190 kb on - strand, within HMPREF1058_RS01490 at 112.240 kb on + strand, within HMPREF1058_RS01490 at 112.240 kb on + strand, within HMPREF1058_RS01490 at 112.243 kb on + strand, within HMPREF1058_RS01490 at 112.244 kb on - strand, within HMPREF1058_RS01490 at 112.248 kb on - strand, within HMPREF1058_RS01490 at 112.253 kb on - strand, within HMPREF1058_RS01490 at 112.309 kb on + strand, within HMPREF1058_RS01490 at 112.337 kb on - strand, within HMPREF1058_RS01490 at 112.494 kb on + strand, within HMPREF1058_RS01490 at 112.566 kb on + strand, within HMPREF1058_RS01490 at 112.566 kb on + strand, within HMPREF1058_RS01490 at 112.626 kb on - strand, within HMPREF1058_RS01490 at 112.828 kb on - strand, within HMPREF1058_RS01490 at 112.837 kb on + strand, within HMPREF1058_RS01490 at 112.838 kb on - strand, within HMPREF1058_RS01490 at 112.838 kb on - strand, within HMPREF1058_RS01490 at 112.865 kb on + strand at 112.938 kb on - strand at 113.036 kb on + strand at 113.036 kb on + strand at 113.067 kb on + strand at 113.067 kb on + strand at 113.103 kb on - strand at 113.170 kb on - strand, within HMPREF1058_RS01485 at 113.170 kb on - strand, within HMPREF1058_RS01485 at 113.214 kb on + strand, within HMPREF1058_RS01485 at 113.221 kb on + strand, within HMPREF1058_RS01485 at 113.223 kb on + strand, within HMPREF1058_RS01485 at 113.224 kb on - strand, within HMPREF1058_RS01485 at 113.255 kb on + strand, within HMPREF1058_RS01485 at 113.256 kb on - strand, within HMPREF1058_RS01485 at 113.274 kb on + strand, within HMPREF1058_RS01485 at 113.274 kb on + strand, within HMPREF1058_RS01485 at 113.383 kb on + strand, within HMPREF1058_RS01485 at 113.384 kb on - strand, within HMPREF1058_RS01485 at 113.479 kb on + strand, within HMPREF1058_RS01485 at 113.525 kb on - strand, within HMPREF1058_RS01485 at 113.525 kb on - strand, within HMPREF1058_RS01485 at 113.525 kb on - strand, within HMPREF1058_RS01485 at 113.527 kb on - strand, within HMPREF1058_RS01485 at 113.527 kb on - strand, within HMPREF1058_RS01485 at 113.586 kb on + strand, within HMPREF1058_RS01485 at 113.587 kb on - strand, within HMPREF1058_RS01485 at 113.600 kb on - strand, within HMPREF1058_RS01485 at 113.605 kb on + strand, within HMPREF1058_RS01485 at 113.692 kb on - strand, within HMPREF1058_RS01485 at 113.744 kb on + strand, within HMPREF1058_RS01485 at 113.770 kb on - strand, within HMPREF1058_RS01485 at 113.842 kb on - strand, within HMPREF1058_RS01485
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 110,993 + HMPREF1058_RS01495 0.70 -0.5 111,097 + HMPREF1058_RS01495 0.74 +2.2 111,164 + HMPREF1058_RS01495 0.77 -1.0 111,180 + HMPREF1058_RS01495 0.78 +0.1 111,181 - HMPREF1058_RS01495 0.78 -1.1 111,203 - HMPREF1058_RS01495 0.79 +0.5 111,205 - HMPREF1058_RS01495 0.79 +0.8 111,205 - HMPREF1058_RS01495 0.79 -0.9 111,266 + HMPREF1058_RS01495 0.81 -0.3 111,356 + HMPREF1058_RS01495 0.85 -0.5 111,381 + HMPREF1058_RS01495 0.86 +0.7 111,381 + HMPREF1058_RS01495 0.86 +0.8 111,423 + HMPREF1058_RS01495 0.88 +0.3 111,426 - HMPREF1058_RS01495 0.88 -1.0 111,435 - HMPREF1058_RS01495 0.89 +0.6 111,539 + +0.1 111,572 - +0.8 111,572 - -0.0 111,576 - +1.6 111,576 - +0.6 111,576 - +0.8 111,576 - +1.1 111,576 - -1.7 111,578 + +0.0 111,663 + -0.1 111,690 + -0.0 111,691 - +0.8 111,702 - -0.8 111,754 - +0.6 111,763 - -0.6 111,772 + -1.5 111,935 + -1.9 111,947 - +0.6 111,973 - -0.5 112,088 - HMPREF1058_RS01490 0.10 -1.7 112,097 - HMPREF1058_RS01490 0.11 -0.3 112,152 - HMPREF1058_RS01490 0.17 +1.0 112,152 - HMPREF1058_RS01490 0.17 +0.6 112,152 - HMPREF1058_RS01490 0.17 +0.4 112,152 - HMPREF1058_RS01490 0.17 -1.2 112,179 + HMPREF1058_RS01490 0.20 -2.7 112,180 - HMPREF1058_RS01490 0.20 -0.7 112,180 - HMPREF1058_RS01490 0.20 +1.2 112,181 + HMPREF1058_RS01490 0.20 -1.6 112,182 - HMPREF1058_RS01490 0.20 -1.4 112,190 - HMPREF1058_RS01490 0.21 +1.2 112,240 + HMPREF1058_RS01490 0.26 -1.9 112,240 + HMPREF1058_RS01490 0.26 +1.6 112,243 + HMPREF1058_RS01490 0.27 +0.6 112,244 - HMPREF1058_RS01490 0.27 -1.2 112,248 - HMPREF1058_RS01490 0.27 -0.3 112,253 - HMPREF1058_RS01490 0.28 +0.2 112,309 + HMPREF1058_RS01490 0.34 -2.0 112,337 - HMPREF1058_RS01490 0.36 +1.2 112,494 + HMPREF1058_RS01490 0.53 -2.1 112,566 + HMPREF1058_RS01490 0.60 -1.4 112,566 + HMPREF1058_RS01490 0.60 +1.6 112,626 - HMPREF1058_RS01490 0.67 -0.0 112,828 - HMPREF1058_RS01490 0.88 -0.6 112,837 + HMPREF1058_RS01490 0.89 -3.0 112,838 - HMPREF1058_RS01490 0.89 +0.2 112,838 - HMPREF1058_RS01490 0.89 -1.8 112,865 + -0.5 112,938 - -2.8 113,036 + +1.4 113,036 + -0.4 113,067 + +1.6 113,067 + -0.6 113,103 - +1.6 113,170 - HMPREF1058_RS01485 0.13 -0.9 113,170 - HMPREF1058_RS01485 0.13 -1.1 113,214 + HMPREF1058_RS01485 0.17 -0.9 113,221 + HMPREF1058_RS01485 0.18 +2.3 113,223 + HMPREF1058_RS01485 0.18 +2.0 113,224 - HMPREF1058_RS01485 0.18 +3.4 113,255 + HMPREF1058_RS01485 0.21 +0.4 113,256 - HMPREF1058_RS01485 0.21 -0.5 113,274 + HMPREF1058_RS01485 0.23 +1.2 113,274 + HMPREF1058_RS01485 0.23 -2.6 113,383 + HMPREF1058_RS01485 0.33 -1.0 113,384 - HMPREF1058_RS01485 0.33 -2.7 113,479 + HMPREF1058_RS01485 0.42 +1.1 113,525 - HMPREF1058_RS01485 0.47 -0.3 113,525 - HMPREF1058_RS01485 0.47 -0.7 113,525 - HMPREF1058_RS01485 0.47 +1.0 113,527 - HMPREF1058_RS01485 0.47 +0.4 113,527 - HMPREF1058_RS01485 0.47 +0.4 113,586 + HMPREF1058_RS01485 0.53 +1.7 113,587 - HMPREF1058_RS01485 0.53 -0.7 113,600 - HMPREF1058_RS01485 0.54 -1.0 113,605 + HMPREF1058_RS01485 0.54 -0.4 113,692 - HMPREF1058_RS01485 0.63 -0.7 113,744 + HMPREF1058_RS01485 0.68 -0.2 113,770 - HMPREF1058_RS01485 0.70 -0.3 113,842 - HMPREF1058_RS01485 0.77 +1.6
Or see this region's nucleotide sequence