Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS18895 and HMPREF1058_RS20200 overlap by 11 nucleotides HMPREF1058_RS20200 and HMPREF1058_RS18890 are separated by 27 nucleotides HMPREF1058_RS18890 and HMPREF1058_RS18885 overlap by 1 nucleotides HMPREF1058_RS18885 and HMPREF1058_RS18880 overlap by 17 nucleotides
HMPREF1058_RS18895: HMPREF1058_RS18895 - hypothetical protein, at 50,718 to 51,155
_RS18895
HMPREF1058_RS20200: HMPREF1058_RS20200 - HNH endonuclease, at 51,145 to 51,759
_RS20200
HMPREF1058_RS18890: HMPREF1058_RS18890 - DUF3127 domain-containing protein, at 51,787 to 52,233
_RS18890
HMPREF1058_RS18885: HMPREF1058_RS18885 - hypothetical protein, at 52,233 to 53,063
_RS18885
HMPREF1058_RS18880: HMPREF1058_RS18880 - ATP-binding protein, at 53,047 to 53,976
_RS18880
Position (kb) 51
52
53 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 50.798 kb on + strand, within HMPREF1058_RS18895 at 51.102 kb on + strand, within HMPREF1058_RS18895 at 51.123 kb on + strand at 51.146 kb on + strand at 51.278 kb on + strand, within HMPREF1058_RS20200 at 51.278 kb on + strand, within HMPREF1058_RS20200 at 51.278 kb on + strand, within HMPREF1058_RS20200 at 51.278 kb on + strand, within HMPREF1058_RS20200 at 51.360 kb on + strand, within HMPREF1058_RS20200 at 51.505 kb on + strand, within HMPREF1058_RS20200 at 51.564 kb on + strand, within HMPREF1058_RS20200 at 51.726 kb on + strand at 51.726 kb on + strand at 51.736 kb on + strand at 51.819 kb on + strand at 51.932 kb on + strand, within HMPREF1058_RS18890 at 51.994 kb on + strand, within HMPREF1058_RS18890 at 52.077 kb on + strand, within HMPREF1058_RS18890 at 52.118 kb on + strand, within HMPREF1058_RS18890 at 52.118 kb on + strand, within HMPREF1058_RS18890 at 52.122 kb on + strand, within HMPREF1058_RS18890 at 52.180 kb on + strand, within HMPREF1058_RS18890 at 52.268 kb on + strand at 52.329 kb on + strand, within HMPREF1058_RS18885 at 52.370 kb on + strand, within HMPREF1058_RS18885 at 52.370 kb on + strand, within HMPREF1058_RS18885 at 52.381 kb on + strand, within HMPREF1058_RS18885 at 52.464 kb on + strand, within HMPREF1058_RS18885 at 52.682 kb on + strand, within HMPREF1058_RS18885 at 52.687 kb on + strand, within HMPREF1058_RS18885 at 52.688 kb on - strand, within HMPREF1058_RS18885 at 52.732 kb on + strand, within HMPREF1058_RS18885 at 52.781 kb on + strand, within HMPREF1058_RS18885 at 52.783 kb on + strand, within HMPREF1058_RS18885 at 52.783 kb on + strand, within HMPREF1058_RS18885 at 52.785 kb on + strand, within HMPREF1058_RS18885 at 52.829 kb on + strand at 52.975 kb on + strand, within HMPREF1058_RS18885 at 53.035 kb on + strand at 53.039 kb on + strand at 53.048 kb on + strand at 53.109 kb on + strand at 53.161 kb on - strand, within HMPREF1058_RS18880
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 50,798 + HMPREF1058_RS18895 0.18 -2.9 51,102 + HMPREF1058_RS18895 0.88 -0.9 51,123 + -0.4 51,146 + -1.9 51,278 + HMPREF1058_RS20200 0.22 +0.1 51,278 + HMPREF1058_RS20200 0.22 -0.0 51,278 + HMPREF1058_RS20200 0.22 -3.2 51,278 + HMPREF1058_RS20200 0.22 +1.8 51,360 + HMPREF1058_RS20200 0.35 +0.2 51,505 + HMPREF1058_RS20200 0.59 -2.1 51,564 + HMPREF1058_RS20200 0.68 -0.2 51,726 + -1.7 51,726 + -0.7 51,736 + +1.1 51,819 + -1.6 51,932 + HMPREF1058_RS18890 0.32 -0.0 51,994 + HMPREF1058_RS18890 0.46 -1.6 52,077 + HMPREF1058_RS18890 0.65 -0.9 52,118 + HMPREF1058_RS18890 0.74 -0.4 52,118 + HMPREF1058_RS18890 0.74 +1.8 52,122 + HMPREF1058_RS18890 0.75 +4.0 52,180 + HMPREF1058_RS18890 0.88 +3.2 52,268 + -1.2 52,329 + HMPREF1058_RS18885 0.12 -0.7 52,370 + HMPREF1058_RS18885 0.16 -0.7 52,370 + HMPREF1058_RS18885 0.16 -0.7 52,381 + HMPREF1058_RS18885 0.18 -1.7 52,464 + HMPREF1058_RS18885 0.28 -0.4 52,682 + HMPREF1058_RS18885 0.54 -1.4 52,687 + HMPREF1058_RS18885 0.55 +0.1 52,688 - HMPREF1058_RS18885 0.55 +0.5 52,732 + HMPREF1058_RS18885 0.60 -0.4 52,781 + HMPREF1058_RS18885 0.66 +0.1 52,783 + HMPREF1058_RS18885 0.66 -0.7 52,783 + HMPREF1058_RS18885 0.66 -0.4 52,785 + HMPREF1058_RS18885 0.66 -0.2 52,829 + +0.6 52,975 + HMPREF1058_RS18885 0.89 -1.4 53,035 + +1.0 53,039 + -0.4 53,048 + -1.5 53,109 + -1.0 53,161 - HMPREF1058_RS18880 0.12 -2.2
Or see this region's nucleotide sequence