Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS17440 and HMPREF1058_RS17435 are separated by 3 nucleotides HMPREF1058_RS17435 and HMPREF1058_RS17430 are separated by 6 nucleotides HMPREF1058_RS17430 and HMPREF1058_RS17425 are separated by 30 nucleotides
HMPREF1058_RS17440: HMPREF1058_RS17440 - DUF3868 domain-containing protein, at 443,271 to 444,365
_RS17440
HMPREF1058_RS17435: HMPREF1058_RS17435 - DUF3575 domain-containing protein, at 444,369 to 444,941
_RS17435
HMPREF1058_RS17430: HMPREF1058_RS17430 - DUF3868 domain-containing protein, at 444,948 to 446,357
_RS17430
HMPREF1058_RS17425: HMPREF1058_RS17425 - Mfa1 family fimbria major subunit, at 446,388 to 447,926
_RS17425
Position (kb) 444
445
446
447 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 444.104 kb on - strand, within HMPREF1058_RS17440 at 444.237 kb on + strand, within HMPREF1058_RS17440 at 444.261 kb on + strand at 444.319 kb on - strand at 444.359 kb on - strand at 444.363 kb on + strand at 444.364 kb on - strand at 444.752 kb on - strand, within HMPREF1058_RS17435 at 444.812 kb on - strand, within HMPREF1058_RS17435 at 444.822 kb on + strand, within HMPREF1058_RS17435 at 444.823 kb on - strand, within HMPREF1058_RS17435 at 444.825 kb on - strand, within HMPREF1058_RS17435 at 444.841 kb on - strand, within HMPREF1058_RS17435 at 444.928 kb on - strand at 444.928 kb on - strand at 444.955 kb on - strand at 444.963 kb on + strand at 444.963 kb on + strand at 444.964 kb on - strand at 444.968 kb on + strand at 444.968 kb on + strand at 445.076 kb on - strand at 445.129 kb on + strand, within HMPREF1058_RS17430 at 445.156 kb on + strand, within HMPREF1058_RS17430 at 445.185 kb on + strand, within HMPREF1058_RS17430 at 445.209 kb on - strand, within HMPREF1058_RS17430 at 445.288 kb on + strand, within HMPREF1058_RS17430 at 445.395 kb on + strand, within HMPREF1058_RS17430 at 445.498 kb on - strand, within HMPREF1058_RS17430 at 445.570 kb on + strand, within HMPREF1058_RS17430 at 445.610 kb on - strand, within HMPREF1058_RS17430 at 445.610 kb on - strand, within HMPREF1058_RS17430 at 445.711 kb on - strand, within HMPREF1058_RS17430 at 445.714 kb on + strand, within HMPREF1058_RS17430 at 445.715 kb on - strand, within HMPREF1058_RS17430 at 445.725 kb on + strand, within HMPREF1058_RS17430 at 445.740 kb on - strand, within HMPREF1058_RS17430 at 445.980 kb on - strand, within HMPREF1058_RS17430 at 446.083 kb on - strand, within HMPREF1058_RS17430 at 446.194 kb on - strand, within HMPREF1058_RS17430 at 446.240 kb on + strand at 446.265 kb on + strand at 446.342 kb on - strand at 446.371 kb on + strand at 446.663 kb on + strand, within HMPREF1058_RS17425 at 446.674 kb on + strand, within HMPREF1058_RS17425 at 446.743 kb on - strand, within HMPREF1058_RS17425 at 446.782 kb on - strand, within HMPREF1058_RS17425 at 446.824 kb on + strand, within HMPREF1058_RS17425 at 446.825 kb on - strand, within HMPREF1058_RS17425 at 446.842 kb on + strand, within HMPREF1058_RS17425 at 446.936 kb on + strand, within HMPREF1058_RS17425 at 447.079 kb on - strand, within HMPREF1058_RS17425 at 447.102 kb on + strand, within HMPREF1058_RS17425 at 447.316 kb on - strand, within HMPREF1058_RS17425
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 444,104 - HMPREF1058_RS17440 0.76 -1.5 444,237 + HMPREF1058_RS17440 0.88 +0.2 444,261 + -0.1 444,319 - -0.5 444,359 - -0.6 444,363 + -0.1 444,364 - -0.5 444,752 - HMPREF1058_RS17435 0.67 -0.2 444,812 - HMPREF1058_RS17435 0.77 +0.5 444,822 + HMPREF1058_RS17435 0.79 -0.1 444,823 - HMPREF1058_RS17435 0.79 +0.5 444,825 - HMPREF1058_RS17435 0.80 +1.5 444,841 - HMPREF1058_RS17435 0.82 -0.8 444,928 - +0.5 444,928 - -0.1 444,955 - +0.1 444,963 + +0.4 444,963 + -0.1 444,964 - +0.8 444,968 + -0.1 444,968 + -0.2 445,076 - -0.8 445,129 + HMPREF1058_RS17430 0.13 +1.7 445,156 + HMPREF1058_RS17430 0.15 +0.4 445,185 + HMPREF1058_RS17430 0.17 +2.5 445,209 - HMPREF1058_RS17430 0.19 +1.5 445,288 + HMPREF1058_RS17430 0.24 -1.5 445,395 + HMPREF1058_RS17430 0.32 +2.3 445,498 - HMPREF1058_RS17430 0.39 +0.8 445,570 + HMPREF1058_RS17430 0.44 +1.7 445,610 - HMPREF1058_RS17430 0.47 -0.8 445,610 - HMPREF1058_RS17430 0.47 -1.3 445,711 - HMPREF1058_RS17430 0.54 -0.3 445,714 + HMPREF1058_RS17430 0.54 +0.7 445,715 - HMPREF1058_RS17430 0.54 -0.6 445,725 + HMPREF1058_RS17430 0.55 +1.9 445,740 - HMPREF1058_RS17430 0.56 +0.1 445,980 - HMPREF1058_RS17430 0.73 +0.1 446,083 - HMPREF1058_RS17430 0.80 -0.8 446,194 - HMPREF1058_RS17430 0.88 +2.9 446,240 + +1.5 446,265 + +2.1 446,342 - -1.6 446,371 + +2.2 446,663 + HMPREF1058_RS17425 0.18 -2.6 446,674 + HMPREF1058_RS17425 0.19 -1.1 446,743 - HMPREF1058_RS17425 0.23 +1.0 446,782 - HMPREF1058_RS17425 0.26 -1.2 446,824 + HMPREF1058_RS17425 0.28 +0.8 446,825 - HMPREF1058_RS17425 0.28 +0.2 446,842 + HMPREF1058_RS17425 0.29 -0.8 446,936 + HMPREF1058_RS17425 0.36 +1.5 447,079 - HMPREF1058_RS17425 0.45 +1.2 447,102 + HMPREF1058_RS17425 0.46 -2.0 447,316 - HMPREF1058_RS17425 0.60 -0.5
Or see this region's nucleotide sequence