Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS15165 and HMPREF1058_RS15160 are separated by 5 nucleotides HMPREF1058_RS15160 and HMPREF1058_RS15155 are separated by 613 nucleotides
HMPREF1058_RS15165: HMPREF1058_RS15165 - galactose mutarotase, at 991,717 to 992,850
_RS15165
HMPREF1058_RS15160: HMPREF1058_RS15160 - beta-galactosidase, at 992,856 to 994,670
_RS15160
HMPREF1058_RS15155: HMPREF1058_RS15155 - AhpC/TSA family protein, at 995,284 to 996,462
_RS15155
Position (kb) 992
993
994
995 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 991.857 kb on - strand, within HMPREF1058_RS15165 at 991.858 kb on + strand, within HMPREF1058_RS15165 at 991.864 kb on - strand, within HMPREF1058_RS15165 at 991.935 kb on - strand, within HMPREF1058_RS15165 at 991.946 kb on - strand, within HMPREF1058_RS15165 at 992.044 kb on + strand, within HMPREF1058_RS15165 at 992.168 kb on + strand, within HMPREF1058_RS15165 at 992.192 kb on + strand, within HMPREF1058_RS15165 at 992.193 kb on - strand, within HMPREF1058_RS15165 at 992.202 kb on + strand, within HMPREF1058_RS15165 at 992.235 kb on - strand, within HMPREF1058_RS15165 at 992.332 kb on - strand, within HMPREF1058_RS15165 at 992.413 kb on - strand, within HMPREF1058_RS15165 at 992.424 kb on + strand, within HMPREF1058_RS15165 at 992.468 kb on + strand, within HMPREF1058_RS15165 at 992.468 kb on + strand, within HMPREF1058_RS15165 at 992.468 kb on + strand, within HMPREF1058_RS15165 at 992.490 kb on - strand, within HMPREF1058_RS15165 at 992.490 kb on - strand, within HMPREF1058_RS15165 at 992.491 kb on + strand, within HMPREF1058_RS15165 at 992.492 kb on - strand, within HMPREF1058_RS15165 at 992.525 kb on - strand, within HMPREF1058_RS15165 at 992.526 kb on + strand, within HMPREF1058_RS15165 at 992.547 kb on + strand, within HMPREF1058_RS15165 at 992.577 kb on + strand, within HMPREF1058_RS15165 at 992.579 kb on + strand, within HMPREF1058_RS15165 at 992.580 kb on - strand, within HMPREF1058_RS15165 at 992.608 kb on + strand, within HMPREF1058_RS15165 at 992.656 kb on - strand, within HMPREF1058_RS15165 at 992.666 kb on + strand, within HMPREF1058_RS15165 at 992.667 kb on - strand, within HMPREF1058_RS15165 at 992.676 kb on + strand, within HMPREF1058_RS15165 at 992.691 kb on + strand, within HMPREF1058_RS15165 at 992.691 kb on + strand, within HMPREF1058_RS15165 at 992.708 kb on - strand, within HMPREF1058_RS15165 at 992.828 kb on - strand at 992.845 kb on - strand at 992.871 kb on - strand at 992.981 kb on - strand at 993.029 kb on + strand at 993.030 kb on - strand at 993.037 kb on - strand at 993.075 kb on + strand, within HMPREF1058_RS15160 at 993.075 kb on + strand, within HMPREF1058_RS15160 at 993.076 kb on - strand, within HMPREF1058_RS15160 at 993.173 kb on - strand, within HMPREF1058_RS15160 at 993.173 kb on - strand, within HMPREF1058_RS15160 at 993.178 kb on + strand, within HMPREF1058_RS15160 at 993.201 kb on - strand, within HMPREF1058_RS15160 at 993.227 kb on + strand, within HMPREF1058_RS15160 at 993.228 kb on - strand, within HMPREF1058_RS15160 at 993.230 kb on - strand, within HMPREF1058_RS15160 at 993.274 kb on + strand, within HMPREF1058_RS15160 at 993.506 kb on + strand, within HMPREF1058_RS15160 at 993.610 kb on - strand, within HMPREF1058_RS15160 at 993.863 kb on + strand, within HMPREF1058_RS15160 at 993.863 kb on + strand, within HMPREF1058_RS15160 at 993.864 kb on - strand, within HMPREF1058_RS15160 at 993.864 kb on - strand, within HMPREF1058_RS15160 at 993.867 kb on + strand, within HMPREF1058_RS15160 at 993.868 kb on - strand, within HMPREF1058_RS15160 at 993.926 kb on - strand, within HMPREF1058_RS15160 at 993.928 kb on - strand, within HMPREF1058_RS15160 at 993.988 kb on - strand, within HMPREF1058_RS15160 at 994.086 kb on - strand, within HMPREF1058_RS15160 at 994.125 kb on - strand, within HMPREF1058_RS15160 at 994.268 kb on + strand, within HMPREF1058_RS15160 at 994.352 kb on - strand, within HMPREF1058_RS15160 at 994.357 kb on - strand, within HMPREF1058_RS15160 at 994.557 kb on - strand at 994.557 kb on - strand at 994.560 kb on + strand at 994.670 kb on + strand at 994.709 kb on + strand at 994.797 kb on + strand at 994.814 kb on - strand at 994.814 kb on - strand at 994.818 kb on - strand at 994.819 kb on + strand at 994.819 kb on + strand at 994.825 kb on + strand at 994.826 kb on - strand at 994.826 kb on - strand at 994.852 kb on + strand at 994.887 kb on + strand at 994.899 kb on + strand at 994.978 kb on + strand at 994.984 kb on - strand at 994.999 kb on - strand at 995.011 kb on + strand at 995.017 kb on - strand at 995.021 kb on - strand at 995.025 kb on + strand at 995.026 kb on - strand at 995.049 kb on + strand at 995.050 kb on - strand at 995.052 kb on - strand at 995.261 kb on + strand at 995.316 kb on + strand at 995.321 kb on + strand at 995.322 kb on - strand at 995.358 kb on + strand at 995.382 kb on + strand at 995.400 kb on + strand at 995.401 kb on - strand at 995.401 kb on - strand at 995.511 kb on + strand, within HMPREF1058_RS15155 at 995.512 kb on - strand, within HMPREF1058_RS15155 at 995.522 kb on + strand, within HMPREF1058_RS15155 at 995.523 kb on - strand, within HMPREF1058_RS15155 at 995.523 kb on - strand, within HMPREF1058_RS15155 at 995.526 kb on + strand, within HMPREF1058_RS15155 at 995.595 kb on + strand, within HMPREF1058_RS15155 at 995.627 kb on + strand, within HMPREF1058_RS15155 at 995.628 kb on - strand, within HMPREF1058_RS15155
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 991,857 - HMPREF1058_RS15165 0.12 -0.2 991,858 + HMPREF1058_RS15165 0.12 +0.2 991,864 - HMPREF1058_RS15165 0.13 -0.8 991,935 - HMPREF1058_RS15165 0.19 +0.2 991,946 - HMPREF1058_RS15165 0.20 +1.1 992,044 + HMPREF1058_RS15165 0.29 -2.0 992,168 + HMPREF1058_RS15165 0.40 -0.9 992,192 + HMPREF1058_RS15165 0.42 +0.3 992,193 - HMPREF1058_RS15165 0.42 -0.5 992,202 + HMPREF1058_RS15165 0.43 +0.2 992,235 - HMPREF1058_RS15165 0.46 +0.6 992,332 - HMPREF1058_RS15165 0.54 -0.9 992,413 - HMPREF1058_RS15165 0.61 +0.4 992,424 + HMPREF1058_RS15165 0.62 -2.0 992,468 + HMPREF1058_RS15165 0.66 +1.4 992,468 + HMPREF1058_RS15165 0.66 +0.2 992,468 + HMPREF1058_RS15165 0.66 -0.8 992,490 - HMPREF1058_RS15165 0.68 -0.7 992,490 - HMPREF1058_RS15165 0.68 -0.9 992,491 + HMPREF1058_RS15165 0.68 +0.1 992,492 - HMPREF1058_RS15165 0.68 +2.5 992,525 - HMPREF1058_RS15165 0.71 -0.4 992,526 + HMPREF1058_RS15165 0.71 -0.6 992,547 + HMPREF1058_RS15165 0.73 -0.7 992,577 + HMPREF1058_RS15165 0.76 +0.0 992,579 + HMPREF1058_RS15165 0.76 -1.7 992,580 - HMPREF1058_RS15165 0.76 -2.1 992,608 + HMPREF1058_RS15165 0.79 -2.1 992,656 - HMPREF1058_RS15165 0.83 -0.5 992,666 + HMPREF1058_RS15165 0.84 -0.1 992,667 - HMPREF1058_RS15165 0.84 -0.2 992,676 + HMPREF1058_RS15165 0.85 -0.8 992,691 + HMPREF1058_RS15165 0.86 -0.2 992,691 + HMPREF1058_RS15165 0.86 -0.1 992,708 - HMPREF1058_RS15165 0.87 +0.8 992,828 - -0.9 992,845 - +1.8 992,871 - -0.9 992,981 - -1.2 993,029 + +1.1 993,030 - -0.8 993,037 - +0.1 993,075 + HMPREF1058_RS15160 0.12 +0.2 993,075 + HMPREF1058_RS15160 0.12 +0.2 993,076 - HMPREF1058_RS15160 0.12 -0.7 993,173 - HMPREF1058_RS15160 0.17 -0.5 993,173 - HMPREF1058_RS15160 0.17 +0.1 993,178 + HMPREF1058_RS15160 0.18 -1.3 993,201 - HMPREF1058_RS15160 0.19 -0.7 993,227 + HMPREF1058_RS15160 0.20 -0.1 993,228 - HMPREF1058_RS15160 0.20 -0.1 993,230 - HMPREF1058_RS15160 0.21 +1.8 993,274 + HMPREF1058_RS15160 0.23 -0.2 993,506 + HMPREF1058_RS15160 0.36 +0.7 993,610 - HMPREF1058_RS15160 0.42 +1.3 993,863 + HMPREF1058_RS15160 0.55 +0.8 993,863 + HMPREF1058_RS15160 0.55 -0.8 993,864 - HMPREF1058_RS15160 0.56 -0.9 993,864 - HMPREF1058_RS15160 0.56 -0.7 993,867 + HMPREF1058_RS15160 0.56 -1.4 993,868 - HMPREF1058_RS15160 0.56 +1.4 993,926 - HMPREF1058_RS15160 0.59 -0.9 993,928 - HMPREF1058_RS15160 0.59 +0.5 993,988 - HMPREF1058_RS15160 0.62 +2.4 994,086 - HMPREF1058_RS15160 0.68 -1.5 994,125 - HMPREF1058_RS15160 0.70 -1.9 994,268 + HMPREF1058_RS15160 0.78 +0.1 994,352 - HMPREF1058_RS15160 0.82 -2.1 994,357 - HMPREF1058_RS15160 0.83 +6.4 994,557 - -0.4 994,557 - -0.4 994,560 + +0.9 994,670 + +0.2 994,709 + -2.9 994,797 + +0.7 994,814 - +1.8 994,814 - -0.5 994,818 - -0.9 994,819 + -0.8 994,819 + -1.4 994,825 + -0.6 994,826 - -0.6 994,826 - -0.8 994,852 + +0.4 994,887 + +0.4 994,899 + -2.8 994,978 + +1.1 994,984 - +0.3 994,999 - -1.1 995,011 + -0.6 995,017 - +0.6 995,021 - -0.2 995,025 + +1.9 995,026 - -0.5 995,049 + +0.7 995,050 - -0.2 995,052 - -2.0 995,261 + -1.0 995,316 + +0.4 995,321 + -0.1 995,322 - +1.4 995,358 + -0.3 995,382 + +0.1 995,400 + +0.9 995,401 - +4.0 995,401 - +0.2 995,511 + HMPREF1058_RS15155 0.19 -0.4 995,512 - HMPREF1058_RS15155 0.19 -0.8 995,522 + HMPREF1058_RS15155 0.20 +1.8 995,523 - HMPREF1058_RS15155 0.20 +0.5 995,523 - HMPREF1058_RS15155 0.20 +1.3 995,526 + HMPREF1058_RS15155 0.21 +0.3 995,595 + HMPREF1058_RS15155 0.26 -1.5 995,627 + HMPREF1058_RS15155 0.29 -0.9 995,628 - HMPREF1058_RS15155 0.29 -2.0
Or see this region's nucleotide sequence