Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS14685 and HMPREF1058_RS14680 overlap by 4 nucleotides HMPREF1058_RS14680 and HMPREF1058_RS14675 are separated by 382 nucleotides
HMPREF1058_RS14685: HMPREF1058_RS14685 - U32 family peptidase, at 1,131,420 to 1,133,249
_RS14685
HMPREF1058_RS14680: HMPREF1058_RS14680 - homoserine O-succinyltransferase, at 1,133,246 to 1,134,163
_RS14680
HMPREF1058_RS14675: HMPREF1058_RS14675 - Gfo/Idh/MocA family oxidoreductase, at 1,134,546 to 1,136,108
_RS14675
Position (kb)
1133
1134
1135 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1132.300 kb on - strand, within HMPREF1058_RS14685 at 1132.358 kb on + strand, within HMPREF1058_RS14685 at 1132.404 kb on + strand, within HMPREF1058_RS14685 at 1132.434 kb on - strand, within HMPREF1058_RS14685 at 1132.436 kb on - strand, within HMPREF1058_RS14685 at 1132.436 kb on - strand, within HMPREF1058_RS14685 at 1132.436 kb on - strand, within HMPREF1058_RS14685 at 1132.501 kb on + strand, within HMPREF1058_RS14685 at 1132.502 kb on - strand, within HMPREF1058_RS14685 at 1132.502 kb on - strand, within HMPREF1058_RS14685 at 1132.502 kb on - strand, within HMPREF1058_RS14685 at 1132.506 kb on + strand, within HMPREF1058_RS14685 at 1132.507 kb on - strand, within HMPREF1058_RS14685 at 1132.602 kb on + strand, within HMPREF1058_RS14685 at 1132.623 kb on - strand, within HMPREF1058_RS14685 at 1132.623 kb on - strand, within HMPREF1058_RS14685 at 1132.641 kb on + strand, within HMPREF1058_RS14685 at 1132.642 kb on - strand, within HMPREF1058_RS14685 at 1132.715 kb on + strand, within HMPREF1058_RS14685 at 1132.720 kb on + strand, within HMPREF1058_RS14685 at 1132.734 kb on - strand, within HMPREF1058_RS14685 at 1132.735 kb on + strand, within HMPREF1058_RS14685 at 1132.736 kb on - strand, within HMPREF1058_RS14685 at 1132.740 kb on + strand, within HMPREF1058_RS14685 at 1132.758 kb on + strand, within HMPREF1058_RS14685 at 1132.887 kb on - strand, within HMPREF1058_RS14685 at 1132.914 kb on + strand, within HMPREF1058_RS14685 at 1132.914 kb on + strand, within HMPREF1058_RS14685 at 1132.923 kb on - strand, within HMPREF1058_RS14685 at 1132.988 kb on - strand, within HMPREF1058_RS14685 at 1133.057 kb on - strand, within HMPREF1058_RS14685 at 1133.069 kb on + strand at 1133.071 kb on + strand at 1133.071 kb on + strand at 1133.071 kb on + strand at 1133.072 kb on - strand at 1133.158 kb on + strand at 1133.158 kb on + strand at 1133.159 kb on - strand at 1133.223 kb on + strand at 1133.261 kb on + strand at 1133.285 kb on + strand at 1133.285 kb on + strand at 1133.285 kb on + strand at 1133.285 kb on + strand at 1133.286 kb on - strand at 1133.286 kb on - strand at 1133.286 kb on - strand at 1133.289 kb on + strand at 1133.289 kb on + strand at 1133.289 kb on + strand at 1133.289 kb on + strand at 1133.293 kb on - strand at 1133.362 kb on + strand, within HMPREF1058_RS14680 at 1133.374 kb on - strand, within HMPREF1058_RS14680 at 1133.422 kb on + strand, within HMPREF1058_RS14680 at 1133.442 kb on + strand, within HMPREF1058_RS14680 at 1133.503 kb on + strand, within HMPREF1058_RS14680 at 1133.503 kb on + strand, within HMPREF1058_RS14680 at 1133.505 kb on + strand, within HMPREF1058_RS14680 at 1133.506 kb on - strand, within HMPREF1058_RS14680 at 1133.506 kb on - strand, within HMPREF1058_RS14680 at 1133.574 kb on - strand, within HMPREF1058_RS14680 at 1133.706 kb on + strand, within HMPREF1058_RS14680 at 1133.707 kb on - strand, within HMPREF1058_RS14680 at 1133.851 kb on + strand, within HMPREF1058_RS14680 at 1133.898 kb on + strand, within HMPREF1058_RS14680 at 1133.911 kb on + strand, within HMPREF1058_RS14680 at 1133.911 kb on + strand, within HMPREF1058_RS14680 at 1133.999 kb on + strand, within HMPREF1058_RS14680 at 1134.107 kb on - strand at 1134.130 kb on + strand at 1134.138 kb on - strand at 1134.186 kb on + strand at 1134.187 kb on - strand at 1134.357 kb on - strand at 1134.459 kb on + strand at 1134.478 kb on + strand at 1134.558 kb on - strand at 1134.572 kb on - strand at 1134.578 kb on - strand at 1134.578 kb on - strand at 1134.581 kb on + strand at 1134.581 kb on + strand at 1134.582 kb on - strand at 1134.582 kb on - strand at 1134.596 kb on - strand at 1134.596 kb on - strand at 1134.599 kb on + strand at 1134.599 kb on + strand at 1134.599 kb on + strand at 1134.599 kb on + strand at 1134.808 kb on - strand, within HMPREF1058_RS14675 at 1134.808 kb on - strand, within HMPREF1058_RS14675 at 1134.809 kb on + strand, within HMPREF1058_RS14675 at 1134.893 kb on + strand, within HMPREF1058_RS14675 at 1134.893 kb on + strand, within HMPREF1058_RS14675 at 1134.940 kb on + strand, within HMPREF1058_RS14675 at 1134.944 kb on + strand, within HMPREF1058_RS14675 at 1134.945 kb on - strand, within HMPREF1058_RS14675 at 1134.950 kb on + strand, within HMPREF1058_RS14675 at 1134.958 kb on + strand, within HMPREF1058_RS14675 at 1134.959 kb on - strand, within HMPREF1058_RS14675 at 1134.965 kb on - strand, within HMPREF1058_RS14675 at 1135.040 kb on + strand, within HMPREF1058_RS14675 at 1135.084 kb on + strand, within HMPREF1058_RS14675 at 1135.085 kb on - strand, within HMPREF1058_RS14675
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 1,132,300 - HMPREF1058_RS14685 0.48 +0.6 1,132,358 + HMPREF1058_RS14685 0.51 -1.0 1,132,404 + HMPREF1058_RS14685 0.54 -0.4 1,132,434 - HMPREF1058_RS14685 0.55 -0.8 1,132,436 - HMPREF1058_RS14685 0.56 +0.9 1,132,436 - HMPREF1058_RS14685 0.56 -1.3 1,132,436 - HMPREF1058_RS14685 0.56 +0.0 1,132,501 + HMPREF1058_RS14685 0.59 -0.3 1,132,502 - HMPREF1058_RS14685 0.59 +0.7 1,132,502 - HMPREF1058_RS14685 0.59 -0.4 1,132,502 - HMPREF1058_RS14685 0.59 -0.6 1,132,506 + HMPREF1058_RS14685 0.59 +0.5 1,132,507 - HMPREF1058_RS14685 0.59 -0.3 1,132,602 + HMPREF1058_RS14685 0.65 +0.0 1,132,623 - HMPREF1058_RS14685 0.66 -0.9 1,132,623 - HMPREF1058_RS14685 0.66 -0.9 1,132,641 + HMPREF1058_RS14685 0.67 +0.9 1,132,642 - HMPREF1058_RS14685 0.67 +0.3 1,132,715 + HMPREF1058_RS14685 0.71 -1.3 1,132,720 + HMPREF1058_RS14685 0.71 -0.5 1,132,734 - HMPREF1058_RS14685 0.72 -0.6 1,132,735 + HMPREF1058_RS14685 0.72 -1.5 1,132,736 - HMPREF1058_RS14685 0.72 -1.6 1,132,740 + HMPREF1058_RS14685 0.72 +0.5 1,132,758 + HMPREF1058_RS14685 0.73 -1.3 1,132,887 - HMPREF1058_RS14685 0.80 -0.1 1,132,914 + HMPREF1058_RS14685 0.82 +0.5 1,132,914 + HMPREF1058_RS14685 0.82 -2.2 1,132,923 - HMPREF1058_RS14685 0.82 +3.3 1,132,988 - HMPREF1058_RS14685 0.86 -1.0 1,133,057 - HMPREF1058_RS14685 0.89 -1.1 1,133,069 + -1.6 1,133,071 + -0.2 1,133,071 + -2.0 1,133,071 + +0.8 1,133,072 - -0.9 1,133,158 + -0.9 1,133,158 + -0.7 1,133,159 - -1.3 1,133,223 + -2.0 1,133,261 + -1.0 1,133,285 + -1.7 1,133,285 + -0.4 1,133,285 + -2.4 1,133,285 + -0.2 1,133,286 - -0.6 1,133,286 - -1.8 1,133,286 - -0.2 1,133,289 + -2.9 1,133,289 + -0.8 1,133,289 + -3.2 1,133,289 + -3.0 1,133,293 - -0.3 1,133,362 + HMPREF1058_RS14680 0.13 -1.2 1,133,374 - HMPREF1058_RS14680 0.14 -2.0 1,133,422 + HMPREF1058_RS14680 0.19 -1.9 1,133,442 + HMPREF1058_RS14680 0.21 -3.7 1,133,503 + HMPREF1058_RS14680 0.28 -1.2 1,133,503 + HMPREF1058_RS14680 0.28 +0.0 1,133,505 + HMPREF1058_RS14680 0.28 -2.7 1,133,506 - HMPREF1058_RS14680 0.28 -0.4 1,133,506 - HMPREF1058_RS14680 0.28 -2.0 1,133,574 - HMPREF1058_RS14680 0.36 -1.0 1,133,706 + HMPREF1058_RS14680 0.50 -3.3 1,133,707 - HMPREF1058_RS14680 0.50 -2.8 1,133,851 + HMPREF1058_RS14680 0.66 -1.3 1,133,898 + HMPREF1058_RS14680 0.71 -1.3 1,133,911 + HMPREF1058_RS14680 0.72 -4.7 1,133,911 + HMPREF1058_RS14680 0.72 -2.5 1,133,999 + HMPREF1058_RS14680 0.82 -0.9 1,134,107 - -3.1 1,134,130 + -3.5 1,134,138 - -0.8 1,134,186 + -2.2 1,134,187 - -1.2 1,134,357 - +1.0 1,134,459 + +0.4 1,134,478 + -0.7 1,134,558 - +0.7 1,134,572 - -0.2 1,134,578 - -1.4 1,134,578 - -1.2 1,134,581 + +0.6 1,134,581 + +1.4 1,134,582 - -1.8 1,134,582 - -2.2 1,134,596 - -1.4 1,134,596 - -0.2 1,134,599 + -1.7 1,134,599 + -2.1 1,134,599 + -0.2 1,134,599 + -0.7 1,134,808 - HMPREF1058_RS14675 0.17 -0.6 1,134,808 - HMPREF1058_RS14675 0.17 -1.1 1,134,809 + HMPREF1058_RS14675 0.17 -0.0 1,134,893 + HMPREF1058_RS14675 0.22 -1.0 1,134,893 + HMPREF1058_RS14675 0.22 -0.7 1,134,940 + HMPREF1058_RS14675 0.25 -0.5 1,134,944 + HMPREF1058_RS14675 0.25 -2.6 1,134,945 - HMPREF1058_RS14675 0.26 -0.7 1,134,950 + HMPREF1058_RS14675 0.26 +1.1 1,134,958 + HMPREF1058_RS14675 0.26 -1.2 1,134,959 - HMPREF1058_RS14675 0.26 +0.6 1,134,965 - HMPREF1058_RS14675 0.27 +0.2 1,135,040 + HMPREF1058_RS14675 0.32 -0.5 1,135,084 + HMPREF1058_RS14675 0.34 -3.0 1,135,085 - HMPREF1058_RS14675 0.34 -2.0
Or see this region's nucleotide sequence