Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS09975 and HMPREF1058_RS09970 are separated by 24 nucleotides HMPREF1058_RS09970 and HMPREF1058_RS09965 are separated by 20 nucleotides HMPREF1058_RS09965 and HMPREF1058_RS09960 overlap by 4 nucleotides HMPREF1058_RS09960 and HMPREF1058_RS09955 are separated by 45 nucleotides HMPREF1058_RS09955 and HMPREF1058_RS20750 are separated by 117 nucleotides HMPREF1058_RS20750 and HMPREF1058_RS09945 are separated by 50 nucleotides
HMPREF1058_RS09975: HMPREF1058_RS09975 - PcfJ family protein, at 753,367 to 754,656
_RS09975
HMPREF1058_RS09970: HMPREF1058_RS09970 - hypothetical protein, at 754,681 to 754,938
_RS09970
HMPREF1058_RS09965: HMPREF1058_RS09965 - DUF3873 domain-containing protein, at 754,959 to 755,192
_RS09965
HMPREF1058_RS09960: HMPREF1058_RS09960 - hypothetical protein, at 755,189 to 755,434
_RS09960
HMPREF1058_RS09955: HMPREF1058_RS09955 - hypothetical protein, at 755,480 to 755,788
_RS09955
HMPREF1058_RS20750: HMPREF1058_RS20750 - hypothetical protein, at 755,906 to 756,127
_RS20750
HMPREF1058_RS09945: HMPREF1058_RS09945 - hypothetical protein, at 756,178 to 757,161
_RS09945
Position (kb) 754
755
756 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 753.959 kb on - strand, within HMPREF1058_RS09975 at 754.107 kb on - strand, within HMPREF1058_RS09975 at 754.107 kb on - strand, within HMPREF1058_RS09975 at 754.111 kb on - strand, within HMPREF1058_RS09975 at 754.160 kb on + strand, within HMPREF1058_RS09975 at 754.205 kb on - strand, within HMPREF1058_RS09975 at 754.246 kb on - strand, within HMPREF1058_RS09975 at 754.246 kb on - strand, within HMPREF1058_RS09975 at 754.344 kb on - strand, within HMPREF1058_RS09975 at 754.655 kb on - strand at 754.658 kb on - strand at 754.688 kb on + strand at 754.843 kb on + strand, within HMPREF1058_RS09970 at 754.867 kb on + strand, within HMPREF1058_RS09970 at 754.985 kb on + strand, within HMPREF1058_RS09965 at 754.988 kb on - strand, within HMPREF1058_RS09965 at 754.988 kb on - strand, within HMPREF1058_RS09965 at 755.035 kb on + strand, within HMPREF1058_RS09965 at 755.036 kb on - strand, within HMPREF1058_RS09965 at 755.150 kb on + strand, within HMPREF1058_RS09965 at 755.201 kb on + strand at 755.233 kb on + strand, within HMPREF1058_RS09960 at 755.344 kb on + strand, within HMPREF1058_RS09960 at 755.508 kb on + strand at 755.728 kb on - strand, within HMPREF1058_RS09955 at 755.811 kb on + strand at 755.816 kb on + strand at 755.965 kb on - strand, within HMPREF1058_RS20750 at 756.014 kb on + strand, within HMPREF1058_RS20750 at 756.023 kb on + strand, within HMPREF1058_RS20750 at 756.023 kb on + strand, within HMPREF1058_RS20750 at 756.023 kb on + strand, within HMPREF1058_RS20750 at 756.023 kb on + strand, within HMPREF1058_RS20750 at 756.023 kb on + strand, within HMPREF1058_RS20750 at 756.025 kb on + strand, within HMPREF1058_RS20750 at 756.179 kb on + strand at 756.180 kb on - strand at 756.181 kb on + strand at 756.182 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 753,959 - HMPREF1058_RS09975 0.46 +0.2 754,107 - HMPREF1058_RS09975 0.57 +0.7 754,107 - HMPREF1058_RS09975 0.57 -1.0 754,111 - HMPREF1058_RS09975 0.58 -1.5 754,160 + HMPREF1058_RS09975 0.61 -0.4 754,205 - HMPREF1058_RS09975 0.65 -1.4 754,246 - HMPREF1058_RS09975 0.68 -1.5 754,246 - HMPREF1058_RS09975 0.68 +1.7 754,344 - HMPREF1058_RS09975 0.76 -0.3 754,655 - -0.1 754,658 - -1.5 754,688 + +0.7 754,843 + HMPREF1058_RS09970 0.63 +0.1 754,867 + HMPREF1058_RS09970 0.72 +0.0 754,985 + HMPREF1058_RS09965 0.11 +0.6 754,988 - HMPREF1058_RS09965 0.12 -0.1 754,988 - HMPREF1058_RS09965 0.12 -1.9 755,035 + HMPREF1058_RS09965 0.32 -0.9 755,036 - HMPREF1058_RS09965 0.33 -1.2 755,150 + HMPREF1058_RS09965 0.82 +2.1 755,201 + -0.9 755,233 + HMPREF1058_RS09960 0.18 -1.0 755,344 + HMPREF1058_RS09960 0.63 -1.4 755,508 + +0.3 755,728 - HMPREF1058_RS09955 0.80 +1.7 755,811 + -0.2 755,816 + +3.0 755,965 - HMPREF1058_RS20750 0.27 +0.8 756,014 + HMPREF1058_RS20750 0.49 -1.0 756,023 + HMPREF1058_RS20750 0.53 -0.5 756,023 + HMPREF1058_RS20750 0.53 -1.1 756,023 + HMPREF1058_RS20750 0.53 -1.0 756,023 + HMPREF1058_RS20750 0.53 -1.2 756,023 + HMPREF1058_RS20750 0.53 -1.7 756,025 + HMPREF1058_RS20750 0.54 +0.2 756,179 + +0.7 756,180 - -1.4 756,181 + +0.8 756,182 - +2.6
Or see this region's nucleotide sequence