Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS08315 and HMPREF1058_RS08310 are separated by 9 nucleotides HMPREF1058_RS08310 and HMPREF1058_RS08305 are separated by 3 nucleotides HMPREF1058_RS08305 and HMPREF1058_RS08300 overlap by 4 nucleotides HMPREF1058_RS08300 and HMPREF1058_RS08295 are separated by 1 nucleotides
HMPREF1058_RS08315: HMPREF1058_RS08315 - biopolymer transporter ExbD, at 1,194,023 to 1,194,442
_RS08315
HMPREF1058_RS08310: HMPREF1058_RS08310 - TonB family protein, at 1,194,452 to 1,195,345
_RS08310
HMPREF1058_RS08305: HMPREF1058_RS08305 - DJ-1/PfpI family protein, at 1,195,349 to 1,195,900
_RS08305
HMPREF1058_RS08300: HMPREF1058_RS08300 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 1,195,897 to 1,196,556
_RS08300
HMPREF1058_RS08295: HMPREF1058_RS08295 - ATP-dependent DNA helicase RecG, at 1,196,558 to 1,198,654
_RS08295
Position (kb)
1195
1196 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1194.447 kb on + strand at 1194.447 kb on + strand at 1194.448 kb on - strand at 1194.463 kb on + strand at 1194.493 kb on - strand at 1194.501 kb on + strand at 1194.501 kb on + strand at 1194.501 kb on + strand at 1194.620 kb on - strand, within HMPREF1058_RS08310 at 1194.633 kb on + strand, within HMPREF1058_RS08310 at 1194.633 kb on + strand, within HMPREF1058_RS08310 at 1194.633 kb on + strand, within HMPREF1058_RS08310 at 1194.633 kb on + strand, within HMPREF1058_RS08310 at 1194.633 kb on + strand, within HMPREF1058_RS08310 at 1194.633 kb on + strand, within HMPREF1058_RS08310 at 1194.634 kb on - strand, within HMPREF1058_RS08310 at 1194.634 kb on - strand, within HMPREF1058_RS08310 at 1194.634 kb on - strand, within HMPREF1058_RS08310 at 1194.638 kb on - strand, within HMPREF1058_RS08310 at 1194.640 kb on - strand, within HMPREF1058_RS08310 at 1194.640 kb on - strand, within HMPREF1058_RS08310 at 1194.673 kb on - strand, within HMPREF1058_RS08310 at 1195.133 kb on + strand, within HMPREF1058_RS08310 at 1195.133 kb on + strand, within HMPREF1058_RS08310 at 1195.176 kb on + strand, within HMPREF1058_RS08310 at 1195.219 kb on + strand, within HMPREF1058_RS08310 at 1195.225 kb on + strand, within HMPREF1058_RS08310 at 1195.315 kb on + strand at 1195.321 kb on + strand at 1195.321 kb on + strand at 1195.325 kb on + strand at 1195.328 kb on + strand at 1195.328 kb on + strand at 1195.348 kb on + strand at 1195.361 kb on + strand at 1195.361 kb on + strand at 1195.524 kb on + strand, within HMPREF1058_RS08305 at 1195.673 kb on + strand, within HMPREF1058_RS08305 at 1195.707 kb on + strand, within HMPREF1058_RS08305 at 1195.707 kb on + strand, within HMPREF1058_RS08305 at 1195.751 kb on + strand, within HMPREF1058_RS08305 at 1196.573 kb on - strand at 1196.708 kb on + strand at 1196.708 kb on + strand at 1196.738 kb on + strand at 1196.738 kb on + strand at 1196.738 kb on + strand at 1196.739 kb on - strand at 1196.739 kb on - strand at 1196.739 kb on - strand at 1196.739 kb on - strand at 1196.880 kb on - strand, within HMPREF1058_RS08295 at 1196.881 kb on + strand, within HMPREF1058_RS08295 at 1196.897 kb on + strand, within HMPREF1058_RS08295 at 1196.898 kb on - strand, within HMPREF1058_RS08295
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 1,194,447 + -0.6 1,194,447 + +0.2 1,194,448 - -2.2 1,194,463 + +0.8 1,194,493 - -2.3 1,194,501 + +1.2 1,194,501 + +0.8 1,194,501 + -2.9 1,194,620 - HMPREF1058_RS08310 0.19 -2.2 1,194,633 + HMPREF1058_RS08310 0.20 -1.6 1,194,633 + HMPREF1058_RS08310 0.20 -2.4 1,194,633 + HMPREF1058_RS08310 0.20 -1.4 1,194,633 + HMPREF1058_RS08310 0.20 -0.8 1,194,633 + HMPREF1058_RS08310 0.20 -1.1 1,194,633 + HMPREF1058_RS08310 0.20 -0.3 1,194,634 - HMPREF1058_RS08310 0.20 -2.0 1,194,634 - HMPREF1058_RS08310 0.20 -0.1 1,194,634 - HMPREF1058_RS08310 0.20 -2.3 1,194,638 - HMPREF1058_RS08310 0.21 -2.5 1,194,640 - HMPREF1058_RS08310 0.21 +0.2 1,194,640 - HMPREF1058_RS08310 0.21 -0.1 1,194,673 - HMPREF1058_RS08310 0.25 -2.2 1,195,133 + HMPREF1058_RS08310 0.76 -1.0 1,195,133 + HMPREF1058_RS08310 0.76 +1.5 1,195,176 + HMPREF1058_RS08310 0.81 -2.0 1,195,219 + HMPREF1058_RS08310 0.86 -1.9 1,195,225 + HMPREF1058_RS08310 0.86 -2.1 1,195,315 + -1.8 1,195,321 + +1.2 1,195,321 + +0.5 1,195,325 + -1.1 1,195,328 + +1.8 1,195,328 + -1.4 1,195,348 + +1.2 1,195,361 + +1.6 1,195,361 + +1.2 1,195,524 + HMPREF1058_RS08305 0.32 +1.8 1,195,673 + HMPREF1058_RS08305 0.59 -1.0 1,195,707 + HMPREF1058_RS08305 0.65 +0.0 1,195,707 + HMPREF1058_RS08305 0.65 -0.7 1,195,751 + HMPREF1058_RS08305 0.73 +2.0 1,196,573 - +1.2 1,196,708 + -1.0 1,196,708 + -1.5 1,196,738 + +0.4 1,196,738 + +0.6 1,196,738 + -1.0 1,196,739 - -1.1 1,196,739 - -1.0 1,196,739 - +1.2 1,196,739 - +0.2 1,196,880 - HMPREF1058_RS08295 0.15 +0.2 1,196,881 + HMPREF1058_RS08295 0.15 +0.6 1,196,897 + HMPREF1058_RS08295 0.16 -0.6 1,196,898 - HMPREF1058_RS08295 0.16 -1.1
Or see this region's nucleotide sequence