Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS05345 and HMPREF1058_RS05340 are separated by 19 nucleotides HMPREF1058_RS05340 and HMPREF1058_RS20985 are separated by 286 nucleotides HMPREF1058_RS20985 and HMPREF1058_RS05335 are separated by 294 nucleotides
HMPREF1058_RS05345: HMPREF1058_RS05345 - site-specific integrase, at 184,979 to 186,196
_RS05345
HMPREF1058_RS05340: HMPREF1058_RS05340 - site-specific integrase, at 186,216 to 187,445
_RS05340
HMPREF1058_RS20985: HMPREF1058_RS20985 - tyrosine-type recombinase/integrase, at 187,732 to 187,899
_RS20985
HMPREF1058_RS05335: HMPREF1058_RS05335 - hypothetical protein, at 188,194 to 188,982
_RS05335
Position (kb) 186
187
188 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 185.544 kb on + strand, within HMPREF1058_RS05345 at 185.545 kb on - strand, within HMPREF1058_RS05345 at 185.545 kb on - strand, within HMPREF1058_RS05345 at 185.545 kb on - strand, within HMPREF1058_RS05345 at 185.628 kb on + strand, within HMPREF1058_RS05345 at 185.629 kb on - strand, within HMPREF1058_RS05345 at 185.868 kb on - strand, within HMPREF1058_RS05345 at 185.974 kb on - strand, within HMPREF1058_RS05345 at 186.032 kb on + strand, within HMPREF1058_RS05345 at 186.108 kb on - strand at 186.150 kb on - strand at 186.166 kb on + strand at 186.168 kb on + strand at 186.168 kb on + strand at 186.169 kb on - strand at 186.173 kb on + strand at 186.174 kb on - strand at 186.177 kb on - strand at 186.220 kb on - strand at 186.299 kb on - strand at 186.517 kb on + strand, within HMPREF1058_RS05340 at 186.600 kb on + strand, within HMPREF1058_RS05340 at 186.622 kb on + strand, within HMPREF1058_RS05340 at 186.674 kb on - strand, within HMPREF1058_RS05340 at 186.808 kb on + strand, within HMPREF1058_RS05340 at 186.808 kb on + strand, within HMPREF1058_RS05340 at 186.808 kb on + strand, within HMPREF1058_RS05340 at 186.938 kb on - strand, within HMPREF1058_RS05340 at 187.523 kb on + strand at 187.524 kb on - strand at 187.753 kb on + strand, within HMPREF1058_RS20985 at 187.753 kb on + strand, within HMPREF1058_RS20985 at 187.754 kb on - strand, within HMPREF1058_RS20985 at 187.850 kb on + strand, within HMPREF1058_RS20985 at 187.887 kb on - strand at 187.900 kb on + strand at 187.958 kb on + strand at 188.002 kb on + strand at 188.006 kb on + strand at 188.085 kb on - strand at 188.096 kb on - strand at 188.096 kb on - strand at 188.100 kb on + strand at 188.228 kb on + strand at 188.228 kb on + strand at 188.228 kb on + strand at 188.231 kb on - strand at 188.317 kb on + strand, within HMPREF1058_RS05335 at 188.318 kb on - strand, within HMPREF1058_RS05335 at 188.318 kb on - strand, within HMPREF1058_RS05335 at 188.344 kb on - strand, within HMPREF1058_RS05335 at 188.348 kb on + strand, within HMPREF1058_RS05335 at 188.421 kb on - strand, within HMPREF1058_RS05335 at 188.437 kb on - strand, within HMPREF1058_RS05335
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 185,544 + HMPREF1058_RS05345 0.46 +0.1 185,545 - HMPREF1058_RS05345 0.46 +0.4 185,545 - HMPREF1058_RS05345 0.46 +0.7 185,545 - HMPREF1058_RS05345 0.46 -1.7 185,628 + HMPREF1058_RS05345 0.53 -1.3 185,629 - HMPREF1058_RS05345 0.53 +0.3 185,868 - HMPREF1058_RS05345 0.73 -2.1 185,974 - HMPREF1058_RS05345 0.82 +4.6 186,032 + HMPREF1058_RS05345 0.86 -0.6 186,108 - -0.1 186,150 - -1.0 186,166 + -1.2 186,168 + -0.3 186,168 + +0.4 186,169 - -0.6 186,173 + +1.1 186,174 - -0.2 186,177 - -0.7 186,220 - +1.1 186,299 - +1.7 186,517 + HMPREF1058_RS05340 0.24 +0.4 186,600 + HMPREF1058_RS05340 0.31 +0.1 186,622 + HMPREF1058_RS05340 0.33 +0.2 186,674 - HMPREF1058_RS05340 0.37 +2.6 186,808 + HMPREF1058_RS05340 0.48 -1.0 186,808 + HMPREF1058_RS05340 0.48 +0.2 186,808 + HMPREF1058_RS05340 0.48 +0.8 186,938 - HMPREF1058_RS05340 0.59 +3.4 187,523 + +0.3 187,524 - +0.8 187,753 + HMPREF1058_RS20985 0.12 +1.9 187,753 + HMPREF1058_RS20985 0.12 +2.7 187,754 - HMPREF1058_RS20985 0.13 -0.9 187,850 + HMPREF1058_RS20985 0.70 +0.7 187,887 - +2.4 187,900 + +1.3 187,958 + +3.1 188,002 + -1.3 188,006 + +2.9 188,085 - +0.1 188,096 - -1.7 188,096 - +0.1 188,100 + +2.1 188,228 + -0.0 188,228 + -0.2 188,228 + -0.6 188,231 - -1.5 188,317 + HMPREF1058_RS05335 0.16 -0.7 188,318 - HMPREF1058_RS05335 0.16 -1.3 188,318 - HMPREF1058_RS05335 0.16 -0.5 188,344 - HMPREF1058_RS05335 0.19 -1.5 188,348 + HMPREF1058_RS05335 0.20 -1.2 188,421 - HMPREF1058_RS05335 0.29 -0.2 188,437 - HMPREF1058_RS05335 0.31 -0.8
Or see this region's nucleotide sequence