Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS18780 and HMPREF1058_RS18775 are separated by 283 nucleotides HMPREF1058_RS18775 and HMPREF1058_RS18770 are separated by 32 nucleotides HMPREF1058_RS18770 and HMPREF1058_RS20375 are separated by 123 nucleotides HMPREF1058_RS20375 and HMPREF1058_RS18765 are separated by 31 nucleotides
HMPREF1058_RS18780: HMPREF1058_RS18780 - aminomethyl-transferring glycine dehydrogenase, at 71,447 to 74,296
_RS18780
HMPREF1058_RS18775: HMPREF1058_RS18775 - S9 family peptidase, at 74,580 to 77,294
_RS18775
HMPREF1058_RS18770: HMPREF1058_RS18770 - hypothetical protein, at 77,327 to 77,563
_RS18770
HMPREF1058_RS20375: HMPREF1058_RS20375 - hypothetical protein, at 77,687 to 77,914
_RS20375
HMPREF1058_RS18765: HMPREF1058_RS18765 - formate--tetrahydrofolate ligase, at 77,946 to 79,613
_RS18765
Position (kb) 74
75
76
77
78 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 73.712 kb on - strand, within HMPREF1058_RS18780 at 73.719 kb on - strand, within HMPREF1058_RS18780 at 73.726 kb on + strand, within HMPREF1058_RS18780 at 73.726 kb on + strand, within HMPREF1058_RS18780 at 73.791 kb on - strand at 73.827 kb on + strand, within HMPREF1058_RS18780 at 73.827 kb on + strand, within HMPREF1058_RS18780 at 73.827 kb on + strand, within HMPREF1058_RS18780 at 73.827 kb on + strand, within HMPREF1058_RS18780 at 73.828 kb on - strand, within HMPREF1058_RS18780 at 73.828 kb on - strand, within HMPREF1058_RS18780 at 73.828 kb on - strand, within HMPREF1058_RS18780 at 73.828 kb on - strand, within HMPREF1058_RS18780 at 73.828 kb on - strand, within HMPREF1058_RS18780 at 73.828 kb on - strand, within HMPREF1058_RS18780 at 74.106 kb on + strand at 74.245 kb on + strand at 74.245 kb on + strand at 74.250 kb on + strand at 74.250 kb on + strand at 74.250 kb on + strand at 74.308 kb on - strand at 74.351 kb on - strand at 74.517 kb on + strand at 74.518 kb on - strand at 74.518 kb on - strand at 74.530 kb on + strand at 74.575 kb on + strand at 74.575 kb on + strand at 74.578 kb on + strand at 74.596 kb on + strand at 74.681 kb on + strand at 74.741 kb on - strand at 74.789 kb on - strand at 74.794 kb on - strand at 74.832 kb on - strand at 74.841 kb on + strand at 74.841 kb on + strand at 74.857 kb on - strand, within HMPREF1058_RS18775 at 74.985 kb on - strand, within HMPREF1058_RS18775 at 75.005 kb on + strand, within HMPREF1058_RS18775 at 75.006 kb on - strand, within HMPREF1058_RS18775 at 75.089 kb on + strand, within HMPREF1058_RS18775 at 75.168 kb on - strand, within HMPREF1058_RS18775 at 75.170 kb on - strand, within HMPREF1058_RS18775 at 75.195 kb on + strand at 75.204 kb on + strand, within HMPREF1058_RS18775 at 75.204 kb on + strand, within HMPREF1058_RS18775 at 75.205 kb on - strand, within HMPREF1058_RS18775 at 75.242 kb on - strand, within HMPREF1058_RS18775 at 75.317 kb on - strand, within HMPREF1058_RS18775 at 75.370 kb on + strand, within HMPREF1058_RS18775 at 75.440 kb on - strand, within HMPREF1058_RS18775 at 75.497 kb on + strand, within HMPREF1058_RS18775 at 75.502 kb on + strand, within HMPREF1058_RS18775 at 75.503 kb on - strand, within HMPREF1058_RS18775 at 75.546 kb on + strand, within HMPREF1058_RS18775 at 75.569 kb on + strand, within HMPREF1058_RS18775 at 75.588 kb on - strand, within HMPREF1058_RS18775 at 75.589 kb on + strand, within HMPREF1058_RS18775 at 75.643 kb on + strand, within HMPREF1058_RS18775 at 75.733 kb on - strand, within HMPREF1058_RS18775 at 75.759 kb on - strand, within HMPREF1058_RS18775 at 75.818 kb on + strand, within HMPREF1058_RS18775 at 75.837 kb on + strand, within HMPREF1058_RS18775 at 75.898 kb on - strand, within HMPREF1058_RS18775 at 76.249 kb on - strand, within HMPREF1058_RS18775 at 76.295 kb on + strand, within HMPREF1058_RS18775 at 76.295 kb on + strand, within HMPREF1058_RS18775 at 76.296 kb on - strand, within HMPREF1058_RS18775 at 76.297 kb on + strand, within HMPREF1058_RS18775 at 76.297 kb on + strand, within HMPREF1058_RS18775 at 76.297 kb on + strand, within HMPREF1058_RS18775 at 76.298 kb on - strand, within HMPREF1058_RS18775 at 76.306 kb on - strand, within HMPREF1058_RS18775 at 76.310 kb on + strand, within HMPREF1058_RS18775 at 76.313 kb on - strand, within HMPREF1058_RS18775 at 76.377 kb on - strand, within HMPREF1058_RS18775 at 76.377 kb on - strand, within HMPREF1058_RS18775 at 76.644 kb on + strand, within HMPREF1058_RS18775 at 76.723 kb on + strand, within HMPREF1058_RS18775 at 77.024 kb on - strand at 77.096 kb on - strand at 77.263 kb on - strand at 77.263 kb on - strand at 77.283 kb on + strand at 77.284 kb on - strand at 77.339 kb on - strand at 77.382 kb on - strand, within HMPREF1058_RS18770 at 77.506 kb on + strand, within HMPREF1058_RS18770 at 77.525 kb on - strand, within HMPREF1058_RS18770 at 77.594 kb on - strand at 77.633 kb on + strand at 77.650 kb on - strand at 77.650 kb on - strand at 77.651 kb on + strand at 77.660 kb on + strand at 77.660 kb on + strand at 77.660 kb on + strand at 77.660 kb on + strand at 77.661 kb on - strand at 77.680 kb on - strand at 77.729 kb on - strand, within HMPREF1058_RS20375 at 77.730 kb on + strand, within HMPREF1058_RS20375 at 77.730 kb on + strand, within HMPREF1058_RS20375 at 77.730 kb on + strand, within HMPREF1058_RS20375 at 77.802 kb on + strand, within HMPREF1058_RS20375 at 77.802 kb on + strand, within HMPREF1058_RS20375 at 77.803 kb on - strand, within HMPREF1058_RS20375 at 77.807 kb on + strand, within HMPREF1058_RS20375 at 77.822 kb on - strand, within HMPREF1058_RS20375 at 77.837 kb on - strand, within HMPREF1058_RS20375 at 77.962 kb on - strand at 77.985 kb on - strand at 78.189 kb on + strand, within HMPREF1058_RS18765 at 78.194 kb on + strand, within HMPREF1058_RS18765 at 78.226 kb on + strand, within HMPREF1058_RS18765 at 78.226 kb on + strand, within HMPREF1058_RS18765 at 78.227 kb on - strand, within HMPREF1058_RS18765 at 78.227 kb on - strand, within HMPREF1058_RS18765 at 78.230 kb on + strand, within HMPREF1058_RS18765 at 78.231 kb on - strand, within HMPREF1058_RS18765 at 78.248 kb on - strand, within HMPREF1058_RS18765 at 78.255 kb on - strand, within HMPREF1058_RS18765 at 78.276 kb on - strand, within HMPREF1058_RS18765
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 73,712 - HMPREF1058_RS18780 0.79 -0.8 73,719 - HMPREF1058_RS18780 0.80 +0.5 73,726 + HMPREF1058_RS18780 0.80 +0.6 73,726 + HMPREF1058_RS18780 0.80 -0.6 73,791 - +0.5 73,827 + HMPREF1058_RS18780 0.84 +0.1 73,827 + HMPREF1058_RS18780 0.84 -0.5 73,827 + HMPREF1058_RS18780 0.84 -1.1 73,827 + HMPREF1058_RS18780 0.84 -0.9 73,828 - HMPREF1058_RS18780 0.84 +5.2 73,828 - HMPREF1058_RS18780 0.84 +0.5 73,828 - HMPREF1058_RS18780 0.84 +1.2 73,828 - HMPREF1058_RS18780 0.84 -1.1 73,828 - HMPREF1058_RS18780 0.84 +1.2 73,828 - HMPREF1058_RS18780 0.84 +0.3 74,106 + +1.5 74,245 + -1.3 74,245 + +1.7 74,250 + +0.6 74,250 + +1.8 74,250 + -0.1 74,308 - +2.1 74,351 - -2.2 74,517 + +5.0 74,518 - -0.1 74,518 - +2.3 74,530 + -0.8 74,575 + +2.0 74,575 + +2.5 74,578 + +1.3 74,596 + +1.3 74,681 + +3.7 74,741 - +3.4 74,789 - +3.7 74,794 - +2.8 74,832 - +4.4 74,841 + +2.5 74,841 + +2.1 74,857 - HMPREF1058_RS18775 0.10 +2.9 74,985 - HMPREF1058_RS18775 0.15 +2.9 75,005 + HMPREF1058_RS18775 0.16 -0.1 75,006 - HMPREF1058_RS18775 0.16 +3.4 75,089 + HMPREF1058_RS18775 0.19 +3.5 75,168 - HMPREF1058_RS18775 0.22 +1.2 75,170 - HMPREF1058_RS18775 0.22 +2.4 75,195 + +0.5 75,204 + HMPREF1058_RS18775 0.23 +1.0 75,204 + HMPREF1058_RS18775 0.23 +2.3 75,205 - HMPREF1058_RS18775 0.23 +3.9 75,242 - HMPREF1058_RS18775 0.24 +2.9 75,317 - HMPREF1058_RS18775 0.27 +2.9 75,370 + HMPREF1058_RS18775 0.29 +2.1 75,440 - HMPREF1058_RS18775 0.32 +0.9 75,497 + HMPREF1058_RS18775 0.34 +2.7 75,502 + HMPREF1058_RS18775 0.34 +4.9 75,503 - HMPREF1058_RS18775 0.34 +2.3 75,546 + HMPREF1058_RS18775 0.36 +0.9 75,569 + HMPREF1058_RS18775 0.36 +2.7 75,588 - HMPREF1058_RS18775 0.37 +2.9 75,589 + HMPREF1058_RS18775 0.37 +0.6 75,643 + HMPREF1058_RS18775 0.39 +2.6 75,733 - HMPREF1058_RS18775 0.42 +2.5 75,759 - HMPREF1058_RS18775 0.43 +3.1 75,818 + HMPREF1058_RS18775 0.46 +1.7 75,837 + HMPREF1058_RS18775 0.46 +5.3 75,898 - HMPREF1058_RS18775 0.49 +3.6 76,249 - HMPREF1058_RS18775 0.61 +2.8 76,295 + HMPREF1058_RS18775 0.63 +2.9 76,295 + HMPREF1058_RS18775 0.63 +2.5 76,296 - HMPREF1058_RS18775 0.63 +0.4 76,297 + HMPREF1058_RS18775 0.63 +2.5 76,297 + HMPREF1058_RS18775 0.63 +2.9 76,297 + HMPREF1058_RS18775 0.63 +1.2 76,298 - HMPREF1058_RS18775 0.63 +0.5 76,306 - HMPREF1058_RS18775 0.64 +2.7 76,310 + HMPREF1058_RS18775 0.64 +1.4 76,313 - HMPREF1058_RS18775 0.64 +3.4 76,377 - HMPREF1058_RS18775 0.66 +1.7 76,377 - HMPREF1058_RS18775 0.66 +2.7 76,644 + HMPREF1058_RS18775 0.76 +2.5 76,723 + HMPREF1058_RS18775 0.79 +1.3 77,024 - +3.5 77,096 - +2.5 77,263 - +3.7 77,263 - +2.5 77,283 + +0.2 77,284 - +4.6 77,339 - +1.5 77,382 - HMPREF1058_RS18770 0.23 -1.1 77,506 + HMPREF1058_RS18770 0.76 +2.9 77,525 - HMPREF1058_RS18770 0.84 +0.2 77,594 - -2.8 77,633 + -1.0 77,650 - -2.9 77,650 - +0.7 77,651 + +0.9 77,660 + +2.2 77,660 + -0.7 77,660 + +1.4 77,660 + +0.7 77,661 - -1.0 77,680 - -2.1 77,729 - HMPREF1058_RS20375 0.18 -0.1 77,730 + HMPREF1058_RS20375 0.19 -2.4 77,730 + HMPREF1058_RS20375 0.19 -0.6 77,730 + HMPREF1058_RS20375 0.19 +2.5 77,802 + HMPREF1058_RS20375 0.50 -1.7 77,802 + HMPREF1058_RS20375 0.50 +0.9 77,803 - HMPREF1058_RS20375 0.51 -0.6 77,807 + HMPREF1058_RS20375 0.53 -0.9 77,822 - HMPREF1058_RS20375 0.59 -0.5 77,837 - HMPREF1058_RS20375 0.66 -2.5 77,962 - -0.7 77,985 - -2.3 78,189 + HMPREF1058_RS18765 0.15 +0.6 78,194 + HMPREF1058_RS18765 0.15 +1.8 78,226 + HMPREF1058_RS18765 0.17 -0.5 78,226 + HMPREF1058_RS18765 0.17 +0.0 78,227 - HMPREF1058_RS18765 0.17 +2.5 78,227 - HMPREF1058_RS18765 0.17 +0.2 78,230 + HMPREF1058_RS18765 0.17 +0.3 78,231 - HMPREF1058_RS18765 0.17 -1.6 78,248 - HMPREF1058_RS18765 0.18 -2.5 78,255 - HMPREF1058_RS18765 0.19 -1.8 78,276 - HMPREF1058_RS18765 0.20 -1.3
Or see this region's nucleotide sequence