Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS0119295 and HMPREF1058_RS18440 are separated by 6 nucleotides HMPREF1058_RS18440 and HMPREF1058_RS18435 are separated by 537 nucleotides
HMPREF1058_RS0119295: HMPREF1058_RS0119295 - Coenzyme F420 hydrogenase/dehydrogenase, beta subunit C-terminal domain, at 153,893 to 155,173
_RS0119295
HMPREF1058_RS18440: HMPREF1058_RS18440 - hypothetical protein, at 155,180 to 156,712
_RS18440
HMPREF1058_RS18435: HMPREF1058_RS18435 - transcriptional regulator, at 157,250 to 158,083
_RS18435
Position (kb) 155
156
157 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 154.201 kb on + strand, within HMPREF1058_RS0119295 at 154.219 kb on + strand, within HMPREF1058_RS0119295 at 154.250 kb on + strand, within HMPREF1058_RS0119295 at 154.250 kb on + strand, within HMPREF1058_RS0119295 at 154.250 kb on + strand, within HMPREF1058_RS0119295 at 154.251 kb on - strand, within HMPREF1058_RS0119295 at 154.310 kb on - strand, within HMPREF1058_RS0119295 at 154.347 kb on + strand, within HMPREF1058_RS0119295 at 154.351 kb on + strand, within HMPREF1058_RS0119295 at 154.366 kb on + strand, within HMPREF1058_RS0119295 at 154.368 kb on + strand at 154.387 kb on + strand, within HMPREF1058_RS0119295 at 154.406 kb on - strand, within HMPREF1058_RS0119295 at 154.415 kb on - strand, within HMPREF1058_RS0119295 at 154.442 kb on - strand, within HMPREF1058_RS0119295 at 154.535 kb on - strand, within HMPREF1058_RS0119295 at 154.599 kb on - strand, within HMPREF1058_RS0119295 at 154.603 kb on + strand, within HMPREF1058_RS0119295 at 154.604 kb on - strand, within HMPREF1058_RS0119295 at 154.627 kb on + strand, within HMPREF1058_RS0119295 at 154.634 kb on + strand, within HMPREF1058_RS0119295 at 154.651 kb on + strand, within HMPREF1058_RS0119295 at 154.653 kb on + strand, within HMPREF1058_RS0119295 at 154.654 kb on - strand, within HMPREF1058_RS0119295 at 154.685 kb on + strand, within HMPREF1058_RS0119295 at 154.751 kb on - strand, within HMPREF1058_RS0119295 at 154.753 kb on - strand, within HMPREF1058_RS0119295 at 154.868 kb on + strand, within HMPREF1058_RS0119295 at 154.957 kb on + strand, within HMPREF1058_RS0119295 at 154.957 kb on + strand, within HMPREF1058_RS0119295 at 154.969 kb on - strand, within HMPREF1058_RS0119295 at 154.970 kb on + strand, within HMPREF1058_RS0119295 at 154.971 kb on - strand, within HMPREF1058_RS0119295 at 154.971 kb on - strand, within HMPREF1058_RS0119295 at 154.984 kb on + strand, within HMPREF1058_RS0119295 at 155.015 kb on + strand, within HMPREF1058_RS0119295 at 155.016 kb on - strand, within HMPREF1058_RS0119295 at 155.028 kb on - strand, within HMPREF1058_RS0119295 at 155.042 kb on - strand, within HMPREF1058_RS0119295 at 155.178 kb on + strand at 155.198 kb on - strand at 155.253 kb on + strand at 155.258 kb on + strand at 155.259 kb on - strand at 155.288 kb on + strand at 155.324 kb on + strand at 155.324 kb on + strand at 155.325 kb on - strand at 155.326 kb on + strand at 155.327 kb on - strand at 155.473 kb on + strand, within HMPREF1058_RS18440 at 155.517 kb on + strand, within HMPREF1058_RS18440 at 155.518 kb on - strand, within HMPREF1058_RS18440 at 155.526 kb on - strand, within HMPREF1058_RS18440 at 155.526 kb on - strand, within HMPREF1058_RS18440 at 155.526 kb on - strand, within HMPREF1058_RS18440 at 155.679 kb on + strand, within HMPREF1058_RS18440 at 155.702 kb on + strand, within HMPREF1058_RS18440 at 155.711 kb on + strand, within HMPREF1058_RS18440 at 155.711 kb on + strand, within HMPREF1058_RS18440 at 155.713 kb on + strand, within HMPREF1058_RS18440 at 155.713 kb on + strand, within HMPREF1058_RS18440 at 155.713 kb on + strand, within HMPREF1058_RS18440 at 155.713 kb on + strand, within HMPREF1058_RS18440 at 155.715 kb on + strand, within HMPREF1058_RS18440 at 155.758 kb on + strand, within HMPREF1058_RS18440 at 155.758 kb on + strand, within HMPREF1058_RS18440 at 155.758 kb on + strand, within HMPREF1058_RS18440 at 155.758 kb on + strand at 155.758 kb on + strand, within HMPREF1058_RS18440 at 155.760 kb on - strand, within HMPREF1058_RS18440 at 155.784 kb on - strand, within HMPREF1058_RS18440 at 155.802 kb on + strand, within HMPREF1058_RS18440 at 155.803 kb on - strand, within HMPREF1058_RS18440 at 155.904 kb on - strand, within HMPREF1058_RS18440 at 155.947 kb on + strand, within HMPREF1058_RS18440 at 155.954 kb on - strand, within HMPREF1058_RS18440 at 155.995 kb on + strand, within HMPREF1058_RS18440 at 155.998 kb on - strand, within HMPREF1058_RS18440 at 155.998 kb on - strand, within HMPREF1058_RS18440 at 155.998 kb on - strand, within HMPREF1058_RS18440 at 155.998 kb on - strand, within HMPREF1058_RS18440 at 156.016 kb on + strand, within HMPREF1058_RS18440 at 156.016 kb on + strand, within HMPREF1058_RS18440 at 156.017 kb on - strand, within HMPREF1058_RS18440 at 156.017 kb on - strand, within HMPREF1058_RS18440 at 156.022 kb on + strand, within HMPREF1058_RS18440 at 156.091 kb on + strand, within HMPREF1058_RS18440 at 156.092 kb on - strand, within HMPREF1058_RS18440 at 156.098 kb on + strand, within HMPREF1058_RS18440 at 156.098 kb on + strand, within HMPREF1058_RS18440 at 156.098 kb on + strand, within HMPREF1058_RS18440 at 156.110 kb on + strand, within HMPREF1058_RS18440 at 156.110 kb on + strand, within HMPREF1058_RS18440 at 156.112 kb on + strand, within HMPREF1058_RS18440 at 156.113 kb on - strand, within HMPREF1058_RS18440 at 156.146 kb on - strand, within HMPREF1058_RS18440 at 156.148 kb on + strand, within HMPREF1058_RS18440 at 156.165 kb on - strand, within HMPREF1058_RS18440 at 156.165 kb on - strand, within HMPREF1058_RS18440 at 156.166 kb on + strand, within HMPREF1058_RS18440 at 156.168 kb on + strand, within HMPREF1058_RS18440 at 156.223 kb on - strand, within HMPREF1058_RS18440 at 156.252 kb on - strand, within HMPREF1058_RS18440 at 156.252 kb on - strand, within HMPREF1058_RS18440 at 156.254 kb on - strand, within HMPREF1058_RS18440 at 156.265 kb on + strand at 156.265 kb on + strand, within HMPREF1058_RS18440 at 156.268 kb on - strand, within HMPREF1058_RS18440 at 156.287 kb on - strand, within HMPREF1058_RS18440 at 156.337 kb on + strand, within HMPREF1058_RS18440 at 156.338 kb on - strand, within HMPREF1058_RS18440 at 156.401 kb on + strand at 156.403 kb on + strand, within HMPREF1058_RS18440 at 156.403 kb on + strand, within HMPREF1058_RS18440 at 156.403 kb on + strand, within HMPREF1058_RS18440 at 156.408 kb on - strand, within HMPREF1058_RS18440 at 156.436 kb on + strand, within HMPREF1058_RS18440 at 156.457 kb on + strand, within HMPREF1058_RS18440 at 156.483 kb on - strand, within HMPREF1058_RS18440 at 156.497 kb on - strand, within HMPREF1058_RS18440 at 156.531 kb on - strand, within HMPREF1058_RS18440 at 156.560 kb on + strand at 156.571 kb on - strand at 156.606 kb on - strand at 156.608 kb on - strand at 156.620 kb on - strand at 156.749 kb on + strand at 156.758 kb on - strand at 156.791 kb on + strand at 156.792 kb on - strand at 156.828 kb on + strand at 156.870 kb on + strand at 156.871 kb on - strand at 156.936 kb on + strand at 156.937 kb on - strand at 156.947 kb on - strand at 156.950 kb on + strand at 156.950 kb on + strand at 156.950 kb on + strand at 156.950 kb on + strand at 156.951 kb on - strand at 156.951 kb on - strand at 156.951 kb on - strand at 156.964 kb on - strand at 156.964 kb on - strand at 157.002 kb on + strand at 157.008 kb on + strand at 157.008 kb on + strand at 157.009 kb on - strand at 157.009 kb on - strand at 157.079 kb on - strand at 157.096 kb on + strand at 157.114 kb on + strand at 157.145 kb on - strand at 157.164 kb on + strand at 157.182 kb on + strand at 157.192 kb on + strand at 157.208 kb on - strand at 157.222 kb on - strand at 157.222 kb on - strand at 157.227 kb on - strand at 157.231 kb on - strand at 157.288 kb on + strand at 157.289 kb on - strand at 157.330 kb on + strand at 157.351 kb on + strand, within HMPREF1058_RS18435 at 157.351 kb on + strand, within HMPREF1058_RS18435 at 157.388 kb on - strand, within HMPREF1058_RS18435 at 157.388 kb on - strand, within HMPREF1058_RS18435 at 157.413 kb on + strand, within HMPREF1058_RS18435 at 157.413 kb on + strand, within HMPREF1058_RS18435 at 157.449 kb on + strand, within HMPREF1058_RS18435 at 157.450 kb on - strand, within HMPREF1058_RS18435 at 157.465 kb on - strand, within HMPREF1058_RS18435 at 157.501 kb on + strand, within HMPREF1058_RS18435 at 157.595 kb on + strand, within HMPREF1058_RS18435 at 157.596 kb on - strand, within HMPREF1058_RS18435 at 157.596 kb on - strand, within HMPREF1058_RS18435
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 154,201 + HMPREF1058_RS0119295 0.24 +1.5 154,219 + HMPREF1058_RS0119295 0.25 +0.2 154,250 + HMPREF1058_RS0119295 0.28 -2.7 154,250 + HMPREF1058_RS0119295 0.28 +0.4 154,250 + HMPREF1058_RS0119295 0.28 -2.3 154,251 - HMPREF1058_RS0119295 0.28 +5.1 154,310 - HMPREF1058_RS0119295 0.33 +2.1 154,347 + HMPREF1058_RS0119295 0.35 +1.0 154,351 + HMPREF1058_RS0119295 0.36 -1.4 154,366 + HMPREF1058_RS0119295 0.37 +1.2 154,368 + +0.2 154,387 + HMPREF1058_RS0119295 0.39 -0.5 154,406 - HMPREF1058_RS0119295 0.40 +1.0 154,415 - HMPREF1058_RS0119295 0.41 +1.9 154,442 - HMPREF1058_RS0119295 0.43 +1.6 154,535 - HMPREF1058_RS0119295 0.50 +0.2 154,599 - HMPREF1058_RS0119295 0.55 +3.5 154,603 + HMPREF1058_RS0119295 0.55 +0.2 154,604 - HMPREF1058_RS0119295 0.56 +0.5 154,627 + HMPREF1058_RS0119295 0.57 +1.5 154,634 + HMPREF1058_RS0119295 0.58 -3.4 154,651 + HMPREF1058_RS0119295 0.59 -2.9 154,653 + HMPREF1058_RS0119295 0.59 -0.1 154,654 - HMPREF1058_RS0119295 0.59 +0.9 154,685 + HMPREF1058_RS0119295 0.62 -0.8 154,751 - HMPREF1058_RS0119295 0.67 +3.0 154,753 - HMPREF1058_RS0119295 0.67 +3.3 154,868 + HMPREF1058_RS0119295 0.76 +0.7 154,957 + HMPREF1058_RS0119295 0.83 +0.7 154,957 + HMPREF1058_RS0119295 0.83 -0.7 154,969 - HMPREF1058_RS0119295 0.84 +1.5 154,970 + HMPREF1058_RS0119295 0.84 +0.1 154,971 - HMPREF1058_RS0119295 0.84 +1.5 154,971 - HMPREF1058_RS0119295 0.84 +1.5 154,984 + HMPREF1058_RS0119295 0.85 -0.9 155,015 + HMPREF1058_RS0119295 0.88 +1.4 155,016 - HMPREF1058_RS0119295 0.88 +4.7 155,028 - HMPREF1058_RS0119295 0.89 +1.7 155,042 - HMPREF1058_RS0119295 0.90 +0.2 155,178 + -0.3 155,198 - +1.1 155,253 + +0.8 155,258 + -0.3 155,259 - +3.4 155,288 + +1.3 155,324 + +0.2 155,324 + +2.5 155,325 - +3.0 155,326 + +0.3 155,327 - +4.7 155,473 + HMPREF1058_RS18440 0.19 +1.1 155,517 + HMPREF1058_RS18440 0.22 -0.8 155,518 - HMPREF1058_RS18440 0.22 +4.1 155,526 - HMPREF1058_RS18440 0.23 +6.7 155,526 - HMPREF1058_RS18440 0.23 +4.3 155,526 - HMPREF1058_RS18440 0.23 +3.1 155,679 + HMPREF1058_RS18440 0.33 -0.3 155,702 + HMPREF1058_RS18440 0.34 +0.5 155,711 + HMPREF1058_RS18440 0.35 -0.0 155,711 + HMPREF1058_RS18440 0.35 -2.0 155,713 + HMPREF1058_RS18440 0.35 -1.1 155,713 + HMPREF1058_RS18440 0.35 -0.3 155,713 + HMPREF1058_RS18440 0.35 +0.5 155,713 + HMPREF1058_RS18440 0.35 -0.0 155,715 + HMPREF1058_RS18440 0.35 +0.2 155,758 + HMPREF1058_RS18440 0.38 +1.2 155,758 + HMPREF1058_RS18440 0.38 +0.2 155,758 + HMPREF1058_RS18440 0.38 +2.5 155,758 + -0.8 155,758 + HMPREF1058_RS18440 0.38 +0.6 155,760 - HMPREF1058_RS18440 0.38 +3.3 155,784 - HMPREF1058_RS18440 0.39 +4.1 155,802 + HMPREF1058_RS18440 0.41 +1.0 155,803 - HMPREF1058_RS18440 0.41 +0.5 155,904 - HMPREF1058_RS18440 0.47 +0.7 155,947 + HMPREF1058_RS18440 0.50 +0.6 155,954 - HMPREF1058_RS18440 0.50 +3.2 155,995 + HMPREF1058_RS18440 0.53 +1.7 155,998 - HMPREF1058_RS18440 0.53 +2.9 155,998 - HMPREF1058_RS18440 0.53 +1.7 155,998 - HMPREF1058_RS18440 0.53 +1.1 155,998 - HMPREF1058_RS18440 0.53 +2.2 156,016 + HMPREF1058_RS18440 0.55 -1.1 156,016 + HMPREF1058_RS18440 0.55 +0.7 156,017 - HMPREF1058_RS18440 0.55 +0.8 156,017 - HMPREF1058_RS18440 0.55 +3.2 156,022 + HMPREF1058_RS18440 0.55 -0.7 156,091 + HMPREF1058_RS18440 0.59 -1.2 156,092 - HMPREF1058_RS18440 0.59 +2.3 156,098 + HMPREF1058_RS18440 0.60 -0.8 156,098 + HMPREF1058_RS18440 0.60 +0.9 156,098 + HMPREF1058_RS18440 0.60 +0.2 156,110 + HMPREF1058_RS18440 0.61 +4.5 156,110 + HMPREF1058_RS18440 0.61 -1.0 156,112 + HMPREF1058_RS18440 0.61 -1.1 156,113 - HMPREF1058_RS18440 0.61 +3.1 156,146 - HMPREF1058_RS18440 0.63 +2.7 156,148 + HMPREF1058_RS18440 0.63 +1.6 156,165 - HMPREF1058_RS18440 0.64 +5.1 156,165 - HMPREF1058_RS18440 0.64 +0.3 156,166 + HMPREF1058_RS18440 0.64 -0.7 156,168 + HMPREF1058_RS18440 0.64 +0.5 156,223 - HMPREF1058_RS18440 0.68 +4.1 156,252 - HMPREF1058_RS18440 0.70 +3.7 156,252 - HMPREF1058_RS18440 0.70 +3.9 156,254 - HMPREF1058_RS18440 0.70 +2.5 156,265 + +4.1 156,265 + HMPREF1058_RS18440 0.71 +0.5 156,268 - HMPREF1058_RS18440 0.71 +3.9 156,287 - HMPREF1058_RS18440 0.72 +1.3 156,337 + HMPREF1058_RS18440 0.75 -0.4 156,338 - HMPREF1058_RS18440 0.76 +2.5 156,401 + -0.7 156,403 + HMPREF1058_RS18440 0.80 -1.7 156,403 + HMPREF1058_RS18440 0.80 +0.2 156,403 + HMPREF1058_RS18440 0.80 -1.0 156,408 - HMPREF1058_RS18440 0.80 +3.4 156,436 + HMPREF1058_RS18440 0.82 +1.6 156,457 + HMPREF1058_RS18440 0.83 +0.2 156,483 - HMPREF1058_RS18440 0.85 +0.8 156,497 - HMPREF1058_RS18440 0.86 +1.8 156,531 - HMPREF1058_RS18440 0.88 +0.9 156,560 + +1.4 156,571 - +0.0 156,606 - +2.8 156,608 - +4.9 156,620 - +1.6 156,749 + +1.3 156,758 - +2.6 156,791 + -1.6 156,792 - +0.3 156,828 + +0.5 156,870 + +1.0 156,871 - +0.3 156,936 + -0.3 156,937 - -0.8 156,947 - +0.6 156,950 + +0.7 156,950 + +0.2 156,950 + -1.4 156,950 + +2.5 156,951 - +0.5 156,951 - +0.8 156,951 - -0.3 156,964 - -0.2 156,964 - -0.3 157,002 + -2.0 157,008 + -0.8 157,008 + -0.3 157,009 - +1.4 157,009 - +0.5 157,079 - -0.3 157,096 + +0.7 157,114 + +0.6 157,145 - +2.5 157,164 + -2.3 157,182 + +0.9 157,192 + -1.7 157,208 - +1.3 157,222 - +0.7 157,222 - -0.5 157,227 - +3.1 157,231 - +1.7 157,288 + -1.8 157,289 - -0.2 157,330 + +0.1 157,351 + HMPREF1058_RS18435 0.12 -1.1 157,351 + HMPREF1058_RS18435 0.12 -0.7 157,388 - HMPREF1058_RS18435 0.17 -0.3 157,388 - HMPREF1058_RS18435 0.17 +1.5 157,413 + HMPREF1058_RS18435 0.20 -1.3 157,413 + HMPREF1058_RS18435 0.20 -0.1 157,449 + HMPREF1058_RS18435 0.24 -1.6 157,450 - HMPREF1058_RS18435 0.24 +0.3 157,465 - HMPREF1058_RS18435 0.26 -0.8 157,501 + HMPREF1058_RS18435 0.30 +0.7 157,595 + HMPREF1058_RS18435 0.41 +0.5 157,596 - HMPREF1058_RS18435 0.41 -0.1 157,596 - HMPREF1058_RS18435 0.41 -0.6
Or see this region's nucleotide sequence