Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS17705 and HMPREF1058_RS17700 are separated by 15 nucleotides HMPREF1058_RS17700 and HMPREF1058_RS17695 are separated by 677 nucleotides HMPREF1058_RS17695 and HMPREF1058_RS17690 are separated by 132 nucleotides HMPREF1058_RS17690 and HMPREF1058_RS17685 are separated by 204 nucleotides
HMPREF1058_RS17705: HMPREF1058_RS17705 - J domain-containing protein, at 364,988 to 365,959
_RS17705
HMPREF1058_RS17700: HMPREF1058_RS17700 - chaperone modulator CbpM, at 365,975 to 366,262
_RS17700
HMPREF1058_RS17695: HMPREF1058_RS17695 - YihY/virulence factor BrkB family protein, at 366,940 to 368,259
_RS17695
HMPREF1058_RS17690: HMPREF1058_RS17690 - riboflavin synthase, at 368,392 to 368,994
_RS17690
HMPREF1058_RS17685: HMPREF1058_RS17685 - helix-turn-helix transcriptional regulator, at 369,199 to 369,774
_RS17685
Position (kb) 366
367
368
369 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 365.941 kb on - strand at 365.950 kb on + strand at 365.992 kb on + strand at 365.995 kb on + strand at 366.141 kb on + strand, within HMPREF1058_RS17700 at 366.141 kb on + strand, within HMPREF1058_RS17700 at 366.163 kb on + strand, within HMPREF1058_RS17700 at 366.205 kb on - strand, within HMPREF1058_RS17700 at 366.357 kb on + strand at 366.359 kb on + strand at 366.411 kb on - strand at 366.412 kb on + strand at 366.413 kb on - strand at 366.413 kb on - strand at 366.413 kb on - strand at 366.502 kb on + strand at 366.912 kb on - strand at 366.912 kb on - strand at 366.914 kb on - strand at 366.914 kb on - strand at 366.987 kb on + strand at 367.031 kb on + strand at 367.032 kb on - strand at 367.036 kb on + strand at 367.036 kb on + strand at 367.037 kb on - strand at 367.038 kb on + strand at 367.089 kb on + strand, within HMPREF1058_RS17695 at 367.117 kb on + strand, within HMPREF1058_RS17695 at 367.118 kb on - strand, within HMPREF1058_RS17695 at 367.118 kb on - strand, within HMPREF1058_RS17695 at 367.157 kb on + strand, within HMPREF1058_RS17695 at 367.348 kb on + strand, within HMPREF1058_RS17695 at 367.355 kb on + strand, within HMPREF1058_RS17695 at 367.372 kb on + strand, within HMPREF1058_RS17695 at 367.374 kb on + strand, within HMPREF1058_RS17695 at 367.374 kb on + strand, within HMPREF1058_RS17695 at 367.374 kb on + strand, within HMPREF1058_RS17695 at 367.374 kb on + strand, within HMPREF1058_RS17695 at 367.375 kb on - strand, within HMPREF1058_RS17695 at 367.452 kb on - strand, within HMPREF1058_RS17695 at 367.456 kb on - strand, within HMPREF1058_RS17695 at 367.456 kb on - strand at 367.485 kb on + strand, within HMPREF1058_RS17695 at 367.606 kb on - strand, within HMPREF1058_RS17695 at 367.634 kb on - strand, within HMPREF1058_RS17695 at 367.634 kb on - strand, within HMPREF1058_RS17695 at 367.691 kb on - strand, within HMPREF1058_RS17695 at 367.706 kb on + strand, within HMPREF1058_RS17695 at 367.720 kb on + strand, within HMPREF1058_RS17695 at 367.732 kb on + strand, within HMPREF1058_RS17695 at 367.732 kb on + strand at 367.732 kb on + strand, within HMPREF1058_RS17695 at 367.755 kb on + strand, within HMPREF1058_RS17695 at 367.756 kb on - strand, within HMPREF1058_RS17695 at 367.816 kb on + strand, within HMPREF1058_RS17695 at 367.866 kb on + strand, within HMPREF1058_RS17695 at 368.011 kb on + strand, within HMPREF1058_RS17695 at 368.038 kb on + strand, within HMPREF1058_RS17695 at 368.050 kb on - strand, within HMPREF1058_RS17695 at 368.090 kb on + strand, within HMPREF1058_RS17695 at 368.090 kb on + strand, within HMPREF1058_RS17695 at 368.095 kb on - strand, within HMPREF1058_RS17695 at 368.223 kb on + strand at 368.223 kb on + strand at 368.240 kb on + strand at 368.240 kb on + strand at 368.240 kb on + strand at 369.037 kb on - strand at 369.157 kb on - strand at 369.159 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 365,941 - +0.1 365,950 + +1.5 365,992 + -0.5 365,995 + +0.1 366,141 + HMPREF1058_RS17700 0.58 -1.3 366,141 + HMPREF1058_RS17700 0.58 -0.1 366,163 + HMPREF1058_RS17700 0.65 +0.4 366,205 - HMPREF1058_RS17700 0.80 +1.5 366,357 + +0.5 366,359 + +2.1 366,411 - +0.7 366,412 + +2.5 366,413 - +0.1 366,413 - -0.5 366,413 - -0.3 366,502 + -2.1 366,912 - -0.5 366,912 - -1.2 366,914 - +0.2 366,914 - -1.4 366,987 + +1.8 367,031 + -1.9 367,032 - -0.5 367,036 + -1.3 367,036 + -1.4 367,037 - +0.1 367,038 + +0.3 367,089 + HMPREF1058_RS17695 0.11 +0.1 367,117 + HMPREF1058_RS17695 0.13 -2.5 367,118 - HMPREF1058_RS17695 0.13 -1.5 367,118 - HMPREF1058_RS17695 0.13 -0.5 367,157 + HMPREF1058_RS17695 0.16 +0.9 367,348 + HMPREF1058_RS17695 0.31 -0.9 367,355 + HMPREF1058_RS17695 0.31 -2.2 367,372 + HMPREF1058_RS17695 0.33 -2.1 367,374 + HMPREF1058_RS17695 0.33 -1.4 367,374 + HMPREF1058_RS17695 0.33 -2.0 367,374 + HMPREF1058_RS17695 0.33 -2.0 367,374 + HMPREF1058_RS17695 0.33 -1.4 367,375 - HMPREF1058_RS17695 0.33 -1.9 367,452 - HMPREF1058_RS17695 0.39 -0.9 367,456 - HMPREF1058_RS17695 0.39 -0.8 367,456 - +1.5 367,485 + HMPREF1058_RS17695 0.41 -1.7 367,606 - HMPREF1058_RS17695 0.50 -1.1 367,634 - HMPREF1058_RS17695 0.53 -1.1 367,634 - HMPREF1058_RS17695 0.53 -2.1 367,691 - HMPREF1058_RS17695 0.57 -0.1 367,706 + HMPREF1058_RS17695 0.58 -0.1 367,720 + HMPREF1058_RS17695 0.59 +0.5 367,732 + HMPREF1058_RS17695 0.60 -2.0 367,732 + +0.9 367,732 + HMPREF1058_RS17695 0.60 +0.1 367,755 + HMPREF1058_RS17695 0.62 -2.2 367,756 - HMPREF1058_RS17695 0.62 +0.0 367,816 + HMPREF1058_RS17695 0.66 -0.7 367,866 + HMPREF1058_RS17695 0.70 -1.0 368,011 + HMPREF1058_RS17695 0.81 -1.4 368,038 + HMPREF1058_RS17695 0.83 -1.9 368,050 - HMPREF1058_RS17695 0.84 -1.0 368,090 + HMPREF1058_RS17695 0.87 -0.9 368,090 + HMPREF1058_RS17695 0.87 -0.9 368,095 - HMPREF1058_RS17695 0.88 -2.8 368,223 + +0.1 368,223 + -1.1 368,240 + -1.9 368,240 + -1.1 368,240 + -0.7 369,037 - -0.7 369,157 - -1.0 369,159 - -1.2
Or see this region's nucleotide sequence