Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS16960 and HMPREF1058_RS16955 are separated by 249 nucleotides HMPREF1058_RS16955 and HMPREF1058_RS16950 are separated by 70 nucleotides HMPREF1058_RS16950 and HMPREF1058_RS16945 are separated by 33 nucleotides HMPREF1058_RS16945 and HMPREF1058_RS16940 are separated by 44 nucleotides
HMPREF1058_RS16960: HMPREF1058_RS16960 - 30S ribosomal protein S1, at 550,909 to 552,705
_RS16960
HMPREF1058_RS16955: HMPREF1058_RS16955 - cold shock domain-containing protein, at 552,955 to 553,401
_RS16955
HMPREF1058_RS16950: HMPREF1058_RS16950 - ribonuclease Z, at 553,472 to 554,401
_RS16950
HMPREF1058_RS16945: HMPREF1058_RS16945 - RNA polymerase sigma factor, at 554,435 to 554,986
_RS16945
HMPREF1058_RS16940: HMPREF1058_RS16940 - hypothetical protein, at 555,031 to 555,408
_RS16940
Position (kb) 553
554
555 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 552.717 kb on + strand at 552.791 kb on - strand at 552.810 kb on + strand at 552.883 kb on + strand at 552.884 kb on - strand at 552.892 kb on - strand at 552.894 kb on + strand at 552.902 kb on + strand at 552.929 kb on + strand at 552.930 kb on - strand at 552.937 kb on + strand at 552.938 kb on - strand at 552.966 kb on + strand at 552.989 kb on + strand at 553.074 kb on + strand, within HMPREF1058_RS16955 at 553.088 kb on + strand, within HMPREF1058_RS16955 at 553.088 kb on + strand, within HMPREF1058_RS16955 at 553.089 kb on - strand, within HMPREF1058_RS16955 at 553.131 kb on + strand, within HMPREF1058_RS16955 at 553.132 kb on - strand, within HMPREF1058_RS16955 at 553.152 kb on + strand, within HMPREF1058_RS16955 at 553.182 kb on + strand, within HMPREF1058_RS16955 at 553.205 kb on + strand, within HMPREF1058_RS16955 at 553.223 kb on + strand, within HMPREF1058_RS16955 at 553.223 kb on + strand, within HMPREF1058_RS16955 at 553.224 kb on - strand, within HMPREF1058_RS16955 at 553.224 kb on - strand, within HMPREF1058_RS16955 at 553.286 kb on - strand, within HMPREF1058_RS16955 at 553.322 kb on + strand, within HMPREF1058_RS16955 at 553.385 kb on + strand at 553.411 kb on - strand at 553.488 kb on + strand at 553.489 kb on - strand at 553.489 kb on - strand at 553.492 kb on + strand at 553.493 kb on - strand at 553.493 kb on - strand at 553.497 kb on + strand at 553.497 kb on + strand at 553.497 kb on + strand at 553.498 kb on - strand at 553.556 kb on - strand at 553.645 kb on + strand, within HMPREF1058_RS16950 at 553.647 kb on + strand, within HMPREF1058_RS16950 at 553.647 kb on + strand, within HMPREF1058_RS16950 at 553.648 kb on - strand, within HMPREF1058_RS16950 at 553.736 kb on + strand, within HMPREF1058_RS16950 at 553.736 kb on + strand, within HMPREF1058_RS16950 at 553.739 kb on - strand, within HMPREF1058_RS16950 at 553.758 kb on + strand, within HMPREF1058_RS16950 at 553.759 kb on - strand, within HMPREF1058_RS16950 at 553.772 kb on - strand, within HMPREF1058_RS16950 at 553.772 kb on - strand, within HMPREF1058_RS16950 at 553.801 kb on + strand, within HMPREF1058_RS16950 at 553.809 kb on - strand, within HMPREF1058_RS16950 at 553.851 kb on + strand, within HMPREF1058_RS16950 at 553.857 kb on - strand, within HMPREF1058_RS16950 at 553.857 kb on - strand, within HMPREF1058_RS16950 at 553.893 kb on + strand, within HMPREF1058_RS16950 at 553.951 kb on + strand, within HMPREF1058_RS16950 at 554.049 kb on + strand, within HMPREF1058_RS16950 at 554.099 kb on + strand, within HMPREF1058_RS16950 at 554.099 kb on + strand, within HMPREF1058_RS16950 at 554.111 kb on - strand, within HMPREF1058_RS16950 at 554.111 kb on - strand, within HMPREF1058_RS16950 at 554.113 kb on + strand, within HMPREF1058_RS16950 at 554.114 kb on - strand, within HMPREF1058_RS16950 at 554.118 kb on - strand, within HMPREF1058_RS16950 at 554.142 kb on + strand, within HMPREF1058_RS16950 at 554.145 kb on - strand, within HMPREF1058_RS16950 at 554.262 kb on + strand, within HMPREF1058_RS16950 at 554.319 kb on + strand at 554.352 kb on + strand at 554.400 kb on - strand at 554.404 kb on + strand at 554.417 kb on + strand at 554.418 kb on - strand at 554.445 kb on - strand at 554.461 kb on - strand at 554.535 kb on + strand, within HMPREF1058_RS16945 at 554.535 kb on + strand, within HMPREF1058_RS16945 at 554.535 kb on + strand, within HMPREF1058_RS16945 at 554.548 kb on - strand, within HMPREF1058_RS16945 at 554.548 kb on - strand, within HMPREF1058_RS16945 at 554.623 kb on - strand, within HMPREF1058_RS16945 at 554.670 kb on + strand, within HMPREF1058_RS16945 at 554.777 kb on + strand, within HMPREF1058_RS16945 at 554.777 kb on + strand, within HMPREF1058_RS16945 at 554.845 kb on + strand, within HMPREF1058_RS16945 at 554.846 kb on - strand, within HMPREF1058_RS16945 at 554.847 kb on + strand, within HMPREF1058_RS16945 at 554.848 kb on - strand, within HMPREF1058_RS16945 at 554.865 kb on - strand, within HMPREF1058_RS16945 at 554.883 kb on - strand, within HMPREF1058_RS16945 at 554.914 kb on + strand, within HMPREF1058_RS16945 at 555.129 kb on + strand, within HMPREF1058_RS16940 at 555.130 kb on - strand, within HMPREF1058_RS16940 at 555.173 kb on + strand, within HMPREF1058_RS16940 at 555.178 kb on - strand, within HMPREF1058_RS16940 at 555.190 kb on - strand, within HMPREF1058_RS16940 at 555.205 kb on + strand, within HMPREF1058_RS16940 at 555.205 kb on + strand, within HMPREF1058_RS16940 at 555.205 kb on + strand, within HMPREF1058_RS16940 at 555.206 kb on - strand, within HMPREF1058_RS16940 at 555.206 kb on - strand, within HMPREF1058_RS16940 at 555.207 kb on + strand, within HMPREF1058_RS16940 at 555.207 kb on + strand, within HMPREF1058_RS16940 at 555.207 kb on + strand, within HMPREF1058_RS16940 at 555.208 kb on - strand, within HMPREF1058_RS16940 at 555.208 kb on - strand, within HMPREF1058_RS16940 at 555.208 kb on - strand, within HMPREF1058_RS16940 at 555.216 kb on + strand, within HMPREF1058_RS16940 at 555.254 kb on + strand, within HMPREF1058_RS16940 at 555.254 kb on + strand, within HMPREF1058_RS16940 at 555.254 kb on + strand, within HMPREF1058_RS16940 at 555.255 kb on - strand, within HMPREF1058_RS16940 at 555.255 kb on - strand, within HMPREF1058_RS16940 at 555.255 kb on - strand at 555.319 kb on + strand, within HMPREF1058_RS16940 at 555.320 kb on - strand, within HMPREF1058_RS16940 at 555.321 kb on + strand, within HMPREF1058_RS16940 at 555.360 kb on + strand, within HMPREF1058_RS16940 at 555.396 kb on + strand at 555.401 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 552,717 + +1.4 552,791 - -0.1 552,810 + +0.6 552,883 + +1.4 552,884 - +0.2 552,892 - -1.2 552,894 + -0.7 552,902 + -1.1 552,929 + -0.8 552,930 - -2.3 552,937 + +0.5 552,938 - -0.0 552,966 + -1.3 552,989 + +0.8 553,074 + HMPREF1058_RS16955 0.27 +1.8 553,088 + HMPREF1058_RS16955 0.30 +0.9 553,088 + HMPREF1058_RS16955 0.30 -0.3 553,089 - HMPREF1058_RS16955 0.30 -0.1 553,131 + HMPREF1058_RS16955 0.39 -1.3 553,132 - HMPREF1058_RS16955 0.40 -0.1 553,152 + HMPREF1058_RS16955 0.44 +0.6 553,182 + HMPREF1058_RS16955 0.51 -0.8 553,205 + HMPREF1058_RS16955 0.56 +2.5 553,223 + HMPREF1058_RS16955 0.60 +0.0 553,223 + HMPREF1058_RS16955 0.60 -0.5 553,224 - HMPREF1058_RS16955 0.60 -0.3 553,224 - HMPREF1058_RS16955 0.60 +0.6 553,286 - HMPREF1058_RS16955 0.74 +1.5 553,322 + HMPREF1058_RS16955 0.82 +0.1 553,385 + +0.5 553,411 - -1.3 553,488 + +0.3 553,489 - -1.0 553,489 - +0.3 553,492 + -1.3 553,493 - -0.7 553,493 - +1.9 553,497 + +0.0 553,497 + +0.4 553,497 + +0.9 553,498 - -0.2 553,556 - -2.4 553,645 + HMPREF1058_RS16950 0.19 -1.3 553,647 + HMPREF1058_RS16950 0.19 +1.5 553,647 + HMPREF1058_RS16950 0.19 +0.9 553,648 - HMPREF1058_RS16950 0.19 +0.9 553,736 + HMPREF1058_RS16950 0.28 +0.7 553,736 + HMPREF1058_RS16950 0.28 +1.8 553,739 - HMPREF1058_RS16950 0.29 -0.1 553,758 + HMPREF1058_RS16950 0.31 -0.1 553,759 - HMPREF1058_RS16950 0.31 -1.9 553,772 - HMPREF1058_RS16950 0.32 +5.1 553,772 - HMPREF1058_RS16950 0.32 -1.0 553,801 + HMPREF1058_RS16950 0.35 +1.5 553,809 - HMPREF1058_RS16950 0.36 +0.1 553,851 + HMPREF1058_RS16950 0.41 +3.9 553,857 - HMPREF1058_RS16950 0.41 -1.1 553,857 - HMPREF1058_RS16950 0.41 -0.0 553,893 + HMPREF1058_RS16950 0.45 +0.8 553,951 + HMPREF1058_RS16950 0.52 +3.0 554,049 + HMPREF1058_RS16950 0.62 +1.5 554,099 + HMPREF1058_RS16950 0.67 +0.6 554,099 + HMPREF1058_RS16950 0.67 +0.5 554,111 - HMPREF1058_RS16950 0.69 +3.5 554,111 - HMPREF1058_RS16950 0.69 +1.9 554,113 + HMPREF1058_RS16950 0.69 -1.3 554,114 - HMPREF1058_RS16950 0.69 +1.0 554,118 - HMPREF1058_RS16950 0.69 +0.1 554,142 + HMPREF1058_RS16950 0.72 +1.1 554,145 - HMPREF1058_RS16950 0.72 +1.7 554,262 + HMPREF1058_RS16950 0.85 -0.3 554,319 + +0.9 554,352 + +0.2 554,400 - +0.2 554,404 + +0.0 554,417 + +0.7 554,418 - +0.7 554,445 - -2.5 554,461 - -1.6 554,535 + HMPREF1058_RS16945 0.18 +0.6 554,535 + HMPREF1058_RS16945 0.18 +0.6 554,535 + HMPREF1058_RS16945 0.18 -1.0 554,548 - HMPREF1058_RS16945 0.20 -0.4 554,548 - HMPREF1058_RS16945 0.20 -0.0 554,623 - HMPREF1058_RS16945 0.34 -0.8 554,670 + HMPREF1058_RS16945 0.43 +0.8 554,777 + HMPREF1058_RS16945 0.62 +0.8 554,777 + HMPREF1058_RS16945 0.62 +1.2 554,845 + HMPREF1058_RS16945 0.74 +1.1 554,846 - HMPREF1058_RS16945 0.74 +1.1 554,847 + HMPREF1058_RS16945 0.75 -1.0 554,848 - HMPREF1058_RS16945 0.75 +0.5 554,865 - HMPREF1058_RS16945 0.78 +1.2 554,883 - HMPREF1058_RS16945 0.81 +4.5 554,914 + HMPREF1058_RS16945 0.87 -2.6 555,129 + HMPREF1058_RS16940 0.26 -1.3 555,130 - HMPREF1058_RS16940 0.26 -2.9 555,173 + HMPREF1058_RS16940 0.38 +0.3 555,178 - HMPREF1058_RS16940 0.39 -0.5 555,190 - HMPREF1058_RS16940 0.42 -1.2 555,205 + HMPREF1058_RS16940 0.46 -1.2 555,205 + HMPREF1058_RS16940 0.46 +2.0 555,205 + HMPREF1058_RS16940 0.46 -0.7 555,206 - HMPREF1058_RS16940 0.46 +0.2 555,206 - HMPREF1058_RS16940 0.46 -1.2 555,207 + HMPREF1058_RS16940 0.47 +0.5 555,207 + HMPREF1058_RS16940 0.47 +0.4 555,207 + HMPREF1058_RS16940 0.47 -1.1 555,208 - HMPREF1058_RS16940 0.47 -1.6 555,208 - HMPREF1058_RS16940 0.47 -1.5 555,208 - HMPREF1058_RS16940 0.47 +0.2 555,216 + HMPREF1058_RS16940 0.49 -2.5 555,254 + HMPREF1058_RS16940 0.59 -2.4 555,254 + HMPREF1058_RS16940 0.59 -0.4 555,254 + HMPREF1058_RS16940 0.59 -1.6 555,255 - HMPREF1058_RS16940 0.59 -3.3 555,255 - HMPREF1058_RS16940 0.59 -1.3 555,255 - -0.3 555,319 + HMPREF1058_RS16940 0.76 -1.7 555,320 - HMPREF1058_RS16940 0.76 -0.2 555,321 + HMPREF1058_RS16940 0.77 -0.9 555,360 + HMPREF1058_RS16940 0.87 +2.1 555,396 + -2.5 555,401 - -1.7
Or see this region's nucleotide sequence