Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS16490 and HMPREF1058_RS16485 are separated by 174 nucleotides HMPREF1058_RS16485 and HMPREF1058_RS16480 are separated by 95 nucleotides HMPREF1058_RS16480 and HMPREF1058_RS16475 are separated by 4 nucleotides
HMPREF1058_RS16490: HMPREF1058_RS16490 - sodium ion-translocating decarboxylase subunit beta, at 657,511 to 658,671
_RS16490
HMPREF1058_RS16485: HMPREF1058_RS16485 - cyclomaltodextrinase C-terminal domain-containing protein, at 658,846 to 659,094
_RS16485
HMPREF1058_RS16480: HMPREF1058_RS16480 - SDR family oxidoreductase, at 659,190 to 659,948
_RS16480
HMPREF1058_RS16475: HMPREF1058_RS16475 - esterase, at 659,953 to 660,771
_RS16475
Position (kb) 658
659
660 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 657.908 kb on - strand, within HMPREF1058_RS16490 at 658.011 kb on - strand, within HMPREF1058_RS16490 at 658.048 kb on + strand, within HMPREF1058_RS16490 at 658.054 kb on + strand, within HMPREF1058_RS16490 at 658.054 kb on + strand at 658.055 kb on - strand, within HMPREF1058_RS16490 at 658.055 kb on - strand, within HMPREF1058_RS16490 at 658.055 kb on - strand, within HMPREF1058_RS16490 at 658.055 kb on - strand, within HMPREF1058_RS16490 at 658.055 kb on - strand, within HMPREF1058_RS16490 at 658.055 kb on - strand, within HMPREF1058_RS16490 at 658.086 kb on + strand, within HMPREF1058_RS16490 at 658.086 kb on + strand, within HMPREF1058_RS16490 at 658.166 kb on + strand, within HMPREF1058_RS16490 at 658.194 kb on + strand, within HMPREF1058_RS16490 at 658.195 kb on - strand, within HMPREF1058_RS16490 at 658.249 kb on - strand, within HMPREF1058_RS16490 at 658.329 kb on - strand, within HMPREF1058_RS16490 at 658.332 kb on - strand at 658.518 kb on - strand, within HMPREF1058_RS16490 at 658.543 kb on - strand, within HMPREF1058_RS16490 at 658.543 kb on - strand, within HMPREF1058_RS16490 at 658.543 kb on - strand, within HMPREF1058_RS16490 at 658.583 kb on - strand at 658.669 kb on + strand at 658.707 kb on + strand at 658.758 kb on + strand at 658.778 kb on + strand at 658.779 kb on - strand at 658.786 kb on + strand at 658.808 kb on - strand at 658.832 kb on + strand at 658.863 kb on + strand at 658.863 kb on + strand at 658.863 kb on + strand at 658.863 kb on + strand at 658.863 kb on + strand at 658.868 kb on - strand at 658.889 kb on + strand, within HMPREF1058_RS16485 at 658.890 kb on - strand, within HMPREF1058_RS16485 at 658.891 kb on + strand, within HMPREF1058_RS16485 at 658.891 kb on + strand, within HMPREF1058_RS16485 at 658.891 kb on + strand, within HMPREF1058_RS16485 at 658.892 kb on - strand, within HMPREF1058_RS16485 at 658.892 kb on - strand, within HMPREF1058_RS16485 at 658.892 kb on - strand, within HMPREF1058_RS16485 at 658.892 kb on - strand, within HMPREF1058_RS16485 at 658.893 kb on + strand, within HMPREF1058_RS16485 at 658.893 kb on + strand, within HMPREF1058_RS16485 at 658.894 kb on - strand, within HMPREF1058_RS16485 at 658.894 kb on - strand, within HMPREF1058_RS16485 at 658.894 kb on - strand, within HMPREF1058_RS16485 at 658.894 kb on - strand, within HMPREF1058_RS16485 at 658.894 kb on - strand, within HMPREF1058_RS16485 at 658.897 kb on + strand, within HMPREF1058_RS16485 at 658.898 kb on - strand, within HMPREF1058_RS16485 at 658.898 kb on - strand, within HMPREF1058_RS16485 at 658.898 kb on - strand, within HMPREF1058_RS16485 at 658.898 kb on - strand, within HMPREF1058_RS16485 at 658.908 kb on + strand, within HMPREF1058_RS16485 at 659.019 kb on + strand, within HMPREF1058_RS16485 at 659.110 kb on - strand at 659.117 kb on + strand at 659.117 kb on + strand at 659.118 kb on - strand at 659.167 kb on - strand at 659.170 kb on + strand at 659.170 kb on + strand at 659.170 kb on + strand at 659.170 kb on + strand at 659.171 kb on - strand at 659.330 kb on + strand, within HMPREF1058_RS16480 at 659.330 kb on + strand, within HMPREF1058_RS16480 at 659.332 kb on + strand, within HMPREF1058_RS16480 at 659.333 kb on - strand, within HMPREF1058_RS16480 at 659.334 kb on + strand, within HMPREF1058_RS16480 at 659.335 kb on - strand, within HMPREF1058_RS16480 at 659.335 kb on - strand, within HMPREF1058_RS16480 at 659.335 kb on - strand, within HMPREF1058_RS16480 at 659.335 kb on - strand, within HMPREF1058_RS16480 at 659.408 kb on + strand, within HMPREF1058_RS16480 at 659.528 kb on + strand, within HMPREF1058_RS16480 at 659.540 kb on + strand, within HMPREF1058_RS16480 at 659.541 kb on - strand, within HMPREF1058_RS16480 at 659.543 kb on + strand, within HMPREF1058_RS16480 at 659.544 kb on - strand, within HMPREF1058_RS16480 at 659.544 kb on - strand, within HMPREF1058_RS16480 at 659.546 kb on + strand, within HMPREF1058_RS16480 at 659.546 kb on + strand, within HMPREF1058_RS16480 at 659.547 kb on - strand, within HMPREF1058_RS16480 at 659.558 kb on + strand, within HMPREF1058_RS16480 at 659.558 kb on + strand, within HMPREF1058_RS16480 at 659.616 kb on - strand, within HMPREF1058_RS16480 at 659.622 kb on + strand, within HMPREF1058_RS16480 at 659.623 kb on - strand, within HMPREF1058_RS16480 at 659.623 kb on - strand, within HMPREF1058_RS16480 at 659.700 kb on + strand, within HMPREF1058_RS16480 at 659.856 kb on + strand, within HMPREF1058_RS16480 at 659.931 kb on - strand at 660.027 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 657,908 - HMPREF1058_RS16490 0.34 +2.0 658,011 - HMPREF1058_RS16490 0.43 +3.0 658,048 + HMPREF1058_RS16490 0.46 -1.3 658,054 + HMPREF1058_RS16490 0.47 +1.4 658,054 + +0.1 658,055 - HMPREF1058_RS16490 0.47 +0.8 658,055 - HMPREF1058_RS16490 0.47 +2.6 658,055 - HMPREF1058_RS16490 0.47 -1.6 658,055 - HMPREF1058_RS16490 0.47 +0.4 658,055 - HMPREF1058_RS16490 0.47 +1.7 658,055 - HMPREF1058_RS16490 0.47 +0.1 658,086 + HMPREF1058_RS16490 0.50 +1.8 658,086 + HMPREF1058_RS16490 0.50 +2.8 658,166 + HMPREF1058_RS16490 0.56 +1.7 658,194 + HMPREF1058_RS16490 0.59 -0.4 658,195 - HMPREF1058_RS16490 0.59 -2.3 658,249 - HMPREF1058_RS16490 0.64 +0.9 658,329 - HMPREF1058_RS16490 0.70 -1.0 658,332 - -0.7 658,518 - HMPREF1058_RS16490 0.87 +1.2 658,543 - HMPREF1058_RS16490 0.89 +3.1 658,543 - HMPREF1058_RS16490 0.89 +2.2 658,543 - HMPREF1058_RS16490 0.89 +0.1 658,583 - -1.2 658,669 + -0.2 658,707 + -0.7 658,758 + -1.1 658,778 + -0.2 658,779 - -1.3 658,786 + -2.6 658,808 - -3.1 658,832 + -0.3 658,863 + -1.0 658,863 + -2.6 658,863 + -1.5 658,863 + -1.0 658,863 + -1.3 658,868 - +0.8 658,889 + HMPREF1058_RS16485 0.17 -1.3 658,890 - HMPREF1058_RS16485 0.18 +0.1 658,891 + HMPREF1058_RS16485 0.18 -2.4 658,891 + HMPREF1058_RS16485 0.18 -2.8 658,891 + HMPREF1058_RS16485 0.18 -2.2 658,892 - HMPREF1058_RS16485 0.18 -0.9 658,892 - HMPREF1058_RS16485 0.18 -1.2 658,892 - HMPREF1058_RS16485 0.18 -1.8 658,892 - HMPREF1058_RS16485 0.18 -1.6 658,893 + HMPREF1058_RS16485 0.19 -4.1 658,893 + HMPREF1058_RS16485 0.19 -1.1 658,894 - HMPREF1058_RS16485 0.19 -0.8 658,894 - HMPREF1058_RS16485 0.19 -2.7 658,894 - HMPREF1058_RS16485 0.19 -1.3 658,894 - HMPREF1058_RS16485 0.19 -0.7 658,894 - HMPREF1058_RS16485 0.19 -1.8 658,897 + HMPREF1058_RS16485 0.20 -1.6 658,898 - HMPREF1058_RS16485 0.21 -0.6 658,898 - HMPREF1058_RS16485 0.21 -3.3 658,898 - HMPREF1058_RS16485 0.21 +0.6 658,898 - HMPREF1058_RS16485 0.21 -2.0 658,908 + HMPREF1058_RS16485 0.25 -1.9 659,019 + HMPREF1058_RS16485 0.69 +0.8 659,110 - -0.2 659,117 + -0.3 659,117 + -0.1 659,118 - +0.1 659,167 - -3.2 659,170 + -1.1 659,170 + +0.5 659,170 + +2.5 659,170 + -0.5 659,171 - -1.9 659,330 + HMPREF1058_RS16480 0.18 -0.7 659,330 + HMPREF1058_RS16480 0.18 -0.8 659,332 + HMPREF1058_RS16480 0.19 -0.0 659,333 - HMPREF1058_RS16480 0.19 -0.5 659,334 + HMPREF1058_RS16480 0.19 -1.5 659,335 - HMPREF1058_RS16480 0.19 +0.1 659,335 - HMPREF1058_RS16480 0.19 +1.9 659,335 - HMPREF1058_RS16480 0.19 +1.3 659,335 - HMPREF1058_RS16480 0.19 +0.8 659,408 + HMPREF1058_RS16480 0.29 +1.5 659,528 + HMPREF1058_RS16480 0.45 -0.7 659,540 + HMPREF1058_RS16480 0.46 -0.8 659,541 - HMPREF1058_RS16480 0.46 +0.9 659,543 + HMPREF1058_RS16480 0.47 +0.6 659,544 - HMPREF1058_RS16480 0.47 -1.8 659,544 - HMPREF1058_RS16480 0.47 +0.4 659,546 + HMPREF1058_RS16480 0.47 -0.2 659,546 + HMPREF1058_RS16480 0.47 -1.2 659,547 - HMPREF1058_RS16480 0.47 -0.3 659,558 + HMPREF1058_RS16480 0.48 -1.0 659,558 + HMPREF1058_RS16480 0.48 -0.3 659,616 - HMPREF1058_RS16480 0.56 -2.2 659,622 + HMPREF1058_RS16480 0.57 -2.2 659,623 - HMPREF1058_RS16480 0.57 +2.3 659,623 - HMPREF1058_RS16480 0.57 +0.1 659,700 + HMPREF1058_RS16480 0.67 -1.7 659,856 + HMPREF1058_RS16480 0.88 -0.4 659,931 - +3.4 660,027 + +0.1
Or see this region's nucleotide sequence