Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS15675 and HMPREF1058_RS15670 are separated by 94 nucleotides HMPREF1058_RS15670 and HMPREF1058_RS15665 are separated by 46 nucleotides
HMPREF1058_RS15675: HMPREF1058_RS15675 - bifunctional folylpolyglutamate synthase/dihydrofolate synthase, at 859,284 to 860,570
_RS15675
HMPREF1058_RS15670: HMPREF1058_RS15670 - galactose mutarotase, at 860,665 to 861,759
_RS15670
HMPREF1058_RS15665: HMPREF1058_RS15665 - MFS transporter, at 861,806 to 863,146
_RS15665
Position (kb) 860
861
862 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 860.620 kb on + strand at 860.636 kb on - strand at 860.638 kb on - strand at 860.661 kb on + strand at 860.800 kb on + strand, within HMPREF1058_RS15670 at 860.816 kb on + strand, within HMPREF1058_RS15670 at 860.817 kb on - strand, within HMPREF1058_RS15670 at 860.817 kb on - strand at 860.821 kb on - strand, within HMPREF1058_RS15670 at 860.848 kb on + strand, within HMPREF1058_RS15670 at 860.849 kb on - strand, within HMPREF1058_RS15670 at 860.849 kb on - strand, within HMPREF1058_RS15670 at 860.874 kb on + strand, within HMPREF1058_RS15670 at 860.877 kb on + strand, within HMPREF1058_RS15670 at 860.878 kb on - strand, within HMPREF1058_RS15670 at 860.879 kb on + strand, within HMPREF1058_RS15670 at 860.879 kb on + strand, within HMPREF1058_RS15670 at 860.890 kb on - strand at 860.980 kb on + strand, within HMPREF1058_RS15670 at 860.980 kb on + strand, within HMPREF1058_RS15670 at 860.980 kb on + strand at 860.991 kb on + strand, within HMPREF1058_RS15670 at 861.155 kb on + strand, within HMPREF1058_RS15670 at 861.159 kb on + strand, within HMPREF1058_RS15670 at 861.215 kb on + strand, within HMPREF1058_RS15670 at 861.216 kb on - strand, within HMPREF1058_RS15670 at 861.310 kb on + strand, within HMPREF1058_RS15670 at 861.329 kb on + strand, within HMPREF1058_RS15670 at 861.329 kb on + strand, within HMPREF1058_RS15670 at 861.359 kb on + strand, within HMPREF1058_RS15670 at 861.460 kb on + strand, within HMPREF1058_RS15670 at 861.460 kb on + strand, within HMPREF1058_RS15670 at 861.460 kb on + strand, within HMPREF1058_RS15670 at 861.465 kb on - strand, within HMPREF1058_RS15670 at 861.544 kb on + strand, within HMPREF1058_RS15670 at 861.549 kb on + strand, within HMPREF1058_RS15670 at 861.571 kb on + strand, within HMPREF1058_RS15670 at 861.573 kb on + strand, within HMPREF1058_RS15670 at 861.573 kb on + strand, within HMPREF1058_RS15670 at 861.574 kb on - strand, within HMPREF1058_RS15670 at 861.718 kb on + strand at 861.771 kb on - strand at 861.776 kb on - strand at 861.783 kb on - strand at 861.830 kb on - strand at 862.009 kb on + strand, within HMPREF1058_RS15665 at 862.059 kb on + strand, within HMPREF1058_RS15665 at 862.088 kb on - strand at 862.111 kb on + strand, within HMPREF1058_RS15665 at 862.111 kb on + strand, within HMPREF1058_RS15665 at 862.112 kb on - strand, within HMPREF1058_RS15665 at 862.113 kb on + strand, within HMPREF1058_RS15665 at 862.114 kb on - strand, within HMPREF1058_RS15665 at 862.119 kb on + strand, within HMPREF1058_RS15665 at 862.136 kb on + strand, within HMPREF1058_RS15665 at 862.137 kb on - strand, within HMPREF1058_RS15665 at 862.156 kb on + strand, within HMPREF1058_RS15665 at 862.163 kb on + strand, within HMPREF1058_RS15665 at 862.197 kb on + strand, within HMPREF1058_RS15665 at 862.201 kb on + strand, within HMPREF1058_RS15665 at 862.374 kb on + strand, within HMPREF1058_RS15665 at 862.375 kb on - strand, within HMPREF1058_RS15665 at 862.516 kb on + strand at 862.524 kb on + strand, within HMPREF1058_RS15665 at 862.524 kb on + strand, within HMPREF1058_RS15665 at 862.530 kb on + strand, within HMPREF1058_RS15665 at 862.548 kb on - strand, within HMPREF1058_RS15665 at 862.551 kb on + strand, within HMPREF1058_RS15665 at 862.552 kb on - strand, within HMPREF1058_RS15665 at 862.589 kb on + strand, within HMPREF1058_RS15665 at 862.655 kb on - strand, within HMPREF1058_RS15665 at 862.667 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 860,620 + +0.4 860,636 - -0.9 860,638 - -0.5 860,661 + -0.7 860,800 + HMPREF1058_RS15670 0.12 -2.7 860,816 + HMPREF1058_RS15670 0.14 -1.7 860,817 - HMPREF1058_RS15670 0.14 -1.5 860,817 - -0.2 860,821 - HMPREF1058_RS15670 0.14 -0.0 860,848 + HMPREF1058_RS15670 0.17 -0.7 860,849 - HMPREF1058_RS15670 0.17 +0.1 860,849 - HMPREF1058_RS15670 0.17 +0.5 860,874 + HMPREF1058_RS15670 0.19 -0.8 860,877 + HMPREF1058_RS15670 0.19 +0.4 860,878 - HMPREF1058_RS15670 0.19 +0.8 860,879 + HMPREF1058_RS15670 0.20 -1.5 860,879 + HMPREF1058_RS15670 0.20 -0.0 860,890 - -0.4 860,980 + HMPREF1058_RS15670 0.29 -0.6 860,980 + HMPREF1058_RS15670 0.29 -1.6 860,980 + -0.2 860,991 + HMPREF1058_RS15670 0.30 +0.4 861,155 + HMPREF1058_RS15670 0.45 -1.8 861,159 + HMPREF1058_RS15670 0.45 -2.2 861,215 + HMPREF1058_RS15670 0.50 -0.9 861,216 - HMPREF1058_RS15670 0.50 -0.1 861,310 + HMPREF1058_RS15670 0.59 -0.4 861,329 + HMPREF1058_RS15670 0.61 -0.2 861,329 + HMPREF1058_RS15670 0.61 -0.3 861,359 + HMPREF1058_RS15670 0.63 -1.1 861,460 + HMPREF1058_RS15670 0.73 +1.7 861,460 + HMPREF1058_RS15670 0.73 +0.4 861,460 + HMPREF1058_RS15670 0.73 -1.0 861,465 - HMPREF1058_RS15670 0.73 -0.2 861,544 + HMPREF1058_RS15670 0.80 +0.5 861,549 + HMPREF1058_RS15670 0.81 +0.9 861,571 + HMPREF1058_RS15670 0.83 +0.1 861,573 + HMPREF1058_RS15670 0.83 +0.7 861,573 + HMPREF1058_RS15670 0.83 +2.8 861,574 - HMPREF1058_RS15670 0.83 +2.4 861,718 + +0.8 861,771 - -0.2 861,776 - +0.6 861,783 - +0.8 861,830 - +1.4 862,009 + HMPREF1058_RS15665 0.15 +0.7 862,059 + HMPREF1058_RS15665 0.19 -1.3 862,088 - +0.1 862,111 + HMPREF1058_RS15665 0.23 -0.2 862,111 + HMPREF1058_RS15665 0.23 -0.4 862,112 - HMPREF1058_RS15665 0.23 +1.6 862,113 + HMPREF1058_RS15665 0.23 -1.2 862,114 - HMPREF1058_RS15665 0.23 +0.9 862,119 + HMPREF1058_RS15665 0.23 +0.2 862,136 + HMPREF1058_RS15665 0.25 -0.3 862,137 - HMPREF1058_RS15665 0.25 +0.4 862,156 + HMPREF1058_RS15665 0.26 -0.1 862,163 + HMPREF1058_RS15665 0.27 -1.3 862,197 + HMPREF1058_RS15665 0.29 -2.6 862,201 + HMPREF1058_RS15665 0.29 +0.8 862,374 + HMPREF1058_RS15665 0.42 -0.4 862,375 - HMPREF1058_RS15665 0.42 -0.6 862,516 + +2.4 862,524 + HMPREF1058_RS15665 0.54 +0.1 862,524 + HMPREF1058_RS15665 0.54 +1.4 862,530 + HMPREF1058_RS15665 0.54 -1.3 862,548 - HMPREF1058_RS15665 0.55 -1.1 862,551 + HMPREF1058_RS15665 0.56 +0.2 862,552 - HMPREF1058_RS15665 0.56 +0.1 862,589 + HMPREF1058_RS15665 0.58 -1.7 862,655 - HMPREF1058_RS15665 0.63 -0.7 862,667 + -0.1
Or see this region's nucleotide sequence