Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS14815 and HMPREF1058_RS14810 are separated by 0 nucleotides HMPREF1058_RS14810 and HMPREF1058_RS14805 are separated by 68 nucleotides HMPREF1058_RS14805 and HMPREF1058_RS14800 are separated by 189 nucleotides
HMPREF1058_RS14815: HMPREF1058_RS14815 - AI-2E family transporter, at 1,097,628 to 1,098,629
_RS14815
HMPREF1058_RS14810: HMPREF1058_RS14810 - pyridoxal phosphate-dependent aminotransferase, at 1,098,630 to 1,099,805
_RS14810
HMPREF1058_RS14805: HMPREF1058_RS14805 - DJ-1/PfpI family protein, at 1,099,874 to 1,100,404
_RS14805
HMPREF1058_RS14800: HMPREF1058_RS14800 - helix-turn-helix transcriptional regulator, at 1,100,594 to 1,100,950
_RS14800
Position (kb)
1098
1099
1100 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1097.683 kb on + strand at 1097.683 kb on + strand at 1097.691 kb on - strand at 1097.696 kb on - strand at 1097.696 kb on - strand at 1097.748 kb on + strand, within HMPREF1058_RS14815 at 1097.759 kb on - strand, within HMPREF1058_RS14815 at 1097.776 kb on - strand, within HMPREF1058_RS14815 at 1097.830 kb on + strand, within HMPREF1058_RS14815 at 1097.855 kb on - strand, within HMPREF1058_RS14815 at 1097.861 kb on - strand at 1097.872 kb on + strand, within HMPREF1058_RS14815 at 1097.872 kb on + strand, within HMPREF1058_RS14815 at 1097.889 kb on - strand, within HMPREF1058_RS14815 at 1097.912 kb on + strand, within HMPREF1058_RS14815 at 1097.960 kb on + strand, within HMPREF1058_RS14815 at 1097.980 kb on + strand, within HMPREF1058_RS14815 at 1098.096 kb on + strand at 1098.138 kb on + strand, within HMPREF1058_RS14815 at 1098.145 kb on + strand, within HMPREF1058_RS14815 at 1098.161 kb on + strand, within HMPREF1058_RS14815 at 1098.217 kb on + strand, within HMPREF1058_RS14815 at 1098.253 kb on + strand, within HMPREF1058_RS14815 at 1098.254 kb on - strand, within HMPREF1058_RS14815 at 1098.257 kb on + strand, within HMPREF1058_RS14815 at 1098.262 kb on + strand, within HMPREF1058_RS14815 at 1098.262 kb on + strand, within HMPREF1058_RS14815 at 1098.263 kb on - strand, within HMPREF1058_RS14815 at 1098.341 kb on - strand, within HMPREF1058_RS14815 at 1098.467 kb on + strand, within HMPREF1058_RS14815 at 1098.636 kb on + strand at 1098.638 kb on + strand at 1098.638 kb on + strand at 1098.638 kb on + strand at 1098.639 kb on - strand at 1098.640 kb on + strand at 1098.640 kb on + strand at 1098.641 kb on - strand at 1098.642 kb on + strand at 1098.687 kb on - strand at 1098.687 kb on - strand at 1098.773 kb on + strand, within HMPREF1058_RS14810 at 1098.841 kb on + strand, within HMPREF1058_RS14810 at 1098.841 kb on + strand, within HMPREF1058_RS14810 at 1098.873 kb on - strand, within HMPREF1058_RS14810 at 1098.873 kb on - strand, within HMPREF1058_RS14810 at 1098.911 kb on + strand, within HMPREF1058_RS14810 at 1098.921 kb on + strand, within HMPREF1058_RS14810 at 1098.921 kb on + strand, within HMPREF1058_RS14810 at 1098.922 kb on - strand, within HMPREF1058_RS14810 at 1098.922 kb on - strand, within HMPREF1058_RS14810 at 1098.925 kb on + strand, within HMPREF1058_RS14810 at 1098.925 kb on + strand, within HMPREF1058_RS14810 at 1098.925 kb on + strand, within HMPREF1058_RS14810 at 1098.925 kb on + strand, within HMPREF1058_RS14810 at 1098.926 kb on - strand, within HMPREF1058_RS14810 at 1098.963 kb on - strand, within HMPREF1058_RS14810 at 1098.965 kb on - strand, within HMPREF1058_RS14810 at 1099.026 kb on - strand, within HMPREF1058_RS14810 at 1099.027 kb on + strand, within HMPREF1058_RS14810 at 1099.028 kb on - strand, within HMPREF1058_RS14810 at 1099.028 kb on - strand, within HMPREF1058_RS14810 at 1099.109 kb on + strand at 1099.119 kb on - strand, within HMPREF1058_RS14810 at 1099.119 kb on - strand, within HMPREF1058_RS14810 at 1099.125 kb on - strand, within HMPREF1058_RS14810 at 1099.176 kb on - strand, within HMPREF1058_RS14810 at 1099.176 kb on - strand, within HMPREF1058_RS14810 at 1099.224 kb on + strand, within HMPREF1058_RS14810 at 1099.225 kb on - strand, within HMPREF1058_RS14810 at 1099.225 kb on - strand, within HMPREF1058_RS14810 at 1099.225 kb on - strand, within HMPREF1058_RS14810 at 1099.322 kb on + strand, within HMPREF1058_RS14810 at 1099.323 kb on - strand, within HMPREF1058_RS14810 at 1099.323 kb on - strand, within HMPREF1058_RS14810 at 1099.595 kb on + strand, within HMPREF1058_RS14810 at 1099.596 kb on - strand, within HMPREF1058_RS14810 at 1099.597 kb on + strand, within HMPREF1058_RS14810 at 1099.597 kb on + strand, within HMPREF1058_RS14810 at 1099.604 kb on + strand, within HMPREF1058_RS14810 at 1099.604 kb on + strand, within HMPREF1058_RS14810 at 1099.649 kb on + strand, within HMPREF1058_RS14810 at 1099.704 kb on + strand at 1099.749 kb on + strand at 1099.780 kb on - strand at 1099.812 kb on + strand at 1099.818 kb on + strand at 1099.836 kb on + strand at 1099.837 kb on - strand at 1099.867 kb on + strand at 1099.991 kb on + strand, within HMPREF1058_RS14805 at 1099.991 kb on + strand, within HMPREF1058_RS14805 at 1099.992 kb on - strand, within HMPREF1058_RS14805 at 1100.137 kb on + strand, within HMPREF1058_RS14805 at 1100.140 kb on - strand, within HMPREF1058_RS14805 at 1100.140 kb on - strand, within HMPREF1058_RS14805 at 1100.140 kb on - strand, within HMPREF1058_RS14805 at 1100.223 kb on - strand, within HMPREF1058_RS14805 at 1100.224 kb on + strand, within HMPREF1058_RS14805 at 1100.301 kb on + strand, within HMPREF1058_RS14805 at 1100.302 kb on - strand, within HMPREF1058_RS14805 at 1100.317 kb on + strand, within HMPREF1058_RS14805 at 1100.416 kb on - strand at 1100.467 kb on - strand at 1100.475 kb on + strand at 1100.543 kb on + strand at 1100.585 kb on + strand at 1100.585 kb on + strand at 1100.638 kb on - strand, within HMPREF1058_RS14800 at 1100.665 kb on + strand, within HMPREF1058_RS14800 at 1100.666 kb on - strand, within HMPREF1058_RS14800 at 1100.666 kb on - strand, within HMPREF1058_RS14800 at 1100.686 kb on - strand, within HMPREF1058_RS14800 at 1100.722 kb on + strand, within HMPREF1058_RS14800 at 1100.749 kb on + strand, within HMPREF1058_RS14800 at 1100.750 kb on - strand, within HMPREF1058_RS14800 at 1100.790 kb on + strand, within HMPREF1058_RS14800
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 1,097,683 + +1.4 1,097,683 + +0.2 1,097,691 - -0.6 1,097,696 - +0.8 1,097,696 - -2.6 1,097,748 + HMPREF1058_RS14815 0.12 -1.0 1,097,759 - HMPREF1058_RS14815 0.13 -1.7 1,097,776 - HMPREF1058_RS14815 0.15 -0.1 1,097,830 + HMPREF1058_RS14815 0.20 -0.9 1,097,855 - HMPREF1058_RS14815 0.23 +0.2 1,097,861 - -0.1 1,097,872 + HMPREF1058_RS14815 0.24 +1.2 1,097,872 + HMPREF1058_RS14815 0.24 -0.8 1,097,889 - HMPREF1058_RS14815 0.26 -1.1 1,097,912 + HMPREF1058_RS14815 0.28 -2.5 1,097,960 + HMPREF1058_RS14815 0.33 +0.2 1,097,980 + HMPREF1058_RS14815 0.35 +0.2 1,098,096 + +0.2 1,098,138 + HMPREF1058_RS14815 0.51 -2.4 1,098,145 + HMPREF1058_RS14815 0.52 -1.2 1,098,161 + HMPREF1058_RS14815 0.53 -0.2 1,098,217 + HMPREF1058_RS14815 0.59 -0.7 1,098,253 + HMPREF1058_RS14815 0.62 -2.5 1,098,254 - HMPREF1058_RS14815 0.62 +0.9 1,098,257 + HMPREF1058_RS14815 0.63 -0.7 1,098,262 + HMPREF1058_RS14815 0.63 -0.0 1,098,262 + HMPREF1058_RS14815 0.63 -0.8 1,098,263 - HMPREF1058_RS14815 0.63 +1.1 1,098,341 - HMPREF1058_RS14815 0.71 -1.7 1,098,467 + HMPREF1058_RS14815 0.84 -0.8 1,098,636 + -0.2 1,098,638 + -0.3 1,098,638 + -0.5 1,098,638 + -0.7 1,098,639 - +0.0 1,098,640 + +0.2 1,098,640 + -0.2 1,098,641 - -0.3 1,098,642 + +0.5 1,098,687 - -1.0 1,098,687 - -1.1 1,098,773 + HMPREF1058_RS14810 0.12 -2.3 1,098,841 + HMPREF1058_RS14810 0.18 +0.5 1,098,841 + HMPREF1058_RS14810 0.18 -2.5 1,098,873 - HMPREF1058_RS14810 0.21 -0.3 1,098,873 - HMPREF1058_RS14810 0.21 +0.8 1,098,911 + HMPREF1058_RS14810 0.24 -0.9 1,098,921 + HMPREF1058_RS14810 0.25 -2.8 1,098,921 + HMPREF1058_RS14810 0.25 +0.6 1,098,922 - HMPREF1058_RS14810 0.25 -2.3 1,098,922 - HMPREF1058_RS14810 0.25 -1.7 1,098,925 + HMPREF1058_RS14810 0.25 -2.8 1,098,925 + HMPREF1058_RS14810 0.25 -2.3 1,098,925 + HMPREF1058_RS14810 0.25 -1.9 1,098,925 + HMPREF1058_RS14810 0.25 -1.1 1,098,926 - HMPREF1058_RS14810 0.25 +0.5 1,098,963 - HMPREF1058_RS14810 0.28 -0.2 1,098,965 - HMPREF1058_RS14810 0.28 -0.7 1,099,026 - HMPREF1058_RS14810 0.34 -1.3 1,099,027 + HMPREF1058_RS14810 0.34 -2.4 1,099,028 - HMPREF1058_RS14810 0.34 -1.6 1,099,028 - HMPREF1058_RS14810 0.34 -2.5 1,099,109 + +0.5 1,099,119 - HMPREF1058_RS14810 0.42 -3.3 1,099,119 - HMPREF1058_RS14810 0.42 -0.5 1,099,125 - HMPREF1058_RS14810 0.42 -0.8 1,099,176 - HMPREF1058_RS14810 0.46 -1.4 1,099,176 - HMPREF1058_RS14810 0.46 -3.1 1,099,224 + HMPREF1058_RS14810 0.51 -1.2 1,099,225 - HMPREF1058_RS14810 0.51 -0.8 1,099,225 - HMPREF1058_RS14810 0.51 -1.5 1,099,225 - HMPREF1058_RS14810 0.51 +0.9 1,099,322 + HMPREF1058_RS14810 0.59 -1.9 1,099,323 - HMPREF1058_RS14810 0.59 -1.1 1,099,323 - HMPREF1058_RS14810 0.59 -0.8 1,099,595 + HMPREF1058_RS14810 0.82 -1.2 1,099,596 - HMPREF1058_RS14810 0.82 -0.3 1,099,597 + HMPREF1058_RS14810 0.82 -1.2 1,099,597 + HMPREF1058_RS14810 0.82 -1.8 1,099,604 + HMPREF1058_RS14810 0.83 -2.9 1,099,604 + HMPREF1058_RS14810 0.83 -0.7 1,099,649 + HMPREF1058_RS14810 0.87 -0.4 1,099,704 + -2.7 1,099,749 + -0.7 1,099,780 - -1.4 1,099,812 + -2.9 1,099,818 + -1.3 1,099,836 + -2.5 1,099,837 - -2.9 1,099,867 + -2.5 1,099,991 + HMPREF1058_RS14805 0.22 -0.1 1,099,991 + HMPREF1058_RS14805 0.22 +0.2 1,099,992 - HMPREF1058_RS14805 0.22 +0.8 1,100,137 + HMPREF1058_RS14805 0.50 -0.3 1,100,140 - HMPREF1058_RS14805 0.50 -0.2 1,100,140 - HMPREF1058_RS14805 0.50 -0.2 1,100,140 - HMPREF1058_RS14805 0.50 -1.7 1,100,223 - HMPREF1058_RS14805 0.66 -0.6 1,100,224 + HMPREF1058_RS14805 0.66 -0.3 1,100,301 + HMPREF1058_RS14805 0.80 -0.7 1,100,302 - HMPREF1058_RS14805 0.81 +0.0 1,100,317 + HMPREF1058_RS14805 0.83 +1.9 1,100,416 - -0.6 1,100,467 - -1.1 1,100,475 + +0.9 1,100,543 + -0.2 1,100,585 + +2.8 1,100,585 + -2.1 1,100,638 - HMPREF1058_RS14800 0.12 +0.7 1,100,665 + HMPREF1058_RS14800 0.20 +1.7 1,100,666 - HMPREF1058_RS14800 0.20 +0.2 1,100,666 - HMPREF1058_RS14800 0.20 +1.5 1,100,686 - HMPREF1058_RS14800 0.26 -0.1 1,100,722 + HMPREF1058_RS14800 0.36 +0.2 1,100,749 + HMPREF1058_RS14800 0.43 +0.5 1,100,750 - HMPREF1058_RS14800 0.44 -0.2 1,100,790 + HMPREF1058_RS14800 0.55 -1.8
Or see this region's nucleotide sequence