Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS14695 and HMPREF1058_RS14690 are separated by 240 nucleotides HMPREF1058_RS14690 and HMPREF1058_RS14685 overlap by 14 nucleotides
HMPREF1058_RS14695: HMPREF1058_RS14695 - TonB-dependent receptor, at 1,127,807 to 1,130,230
_RS14695
HMPREF1058_RS14690: HMPREF1058_RS14690 - zinc ribbon domain-containing protein, at 1,130,471 to 1,131,433
_RS14690
HMPREF1058_RS14685: HMPREF1058_RS14685 - U32 family peptidase, at 1,131,420 to 1,133,249
_RS14685
Position (kb)
1130
1131
1132 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1130.214 kb on + strand at 1130.215 kb on - strand at 1130.229 kb on - strand at 1130.312 kb on - strand at 1130.354 kb on - strand at 1130.385 kb on - strand at 1130.394 kb on - strand at 1130.402 kb on + strand at 1130.403 kb on - strand at 1130.403 kb on - strand at 1130.403 kb on - strand at 1130.423 kb on - strand at 1130.510 kb on - strand at 1130.569 kb on + strand, within HMPREF1058_RS14690 at 1130.594 kb on + strand, within HMPREF1058_RS14690 at 1130.619 kb on + strand, within HMPREF1058_RS14690 at 1130.619 kb on + strand, within HMPREF1058_RS14690 at 1130.622 kb on + strand, within HMPREF1058_RS14690 at 1130.622 kb on + strand, within HMPREF1058_RS14690 at 1130.626 kb on - strand, within HMPREF1058_RS14690 at 1130.633 kb on - strand, within HMPREF1058_RS14690 at 1130.633 kb on - strand, within HMPREF1058_RS14690 at 1130.661 kb on - strand, within HMPREF1058_RS14690 at 1130.704 kb on + strand, within HMPREF1058_RS14690 at 1130.734 kb on + strand, within HMPREF1058_RS14690 at 1130.752 kb on + strand, within HMPREF1058_RS14690 at 1130.765 kb on - strand, within HMPREF1058_RS14690 at 1130.765 kb on - strand, within HMPREF1058_RS14690 at 1130.765 kb on - strand, within HMPREF1058_RS14690 at 1130.788 kb on + strand, within HMPREF1058_RS14690 at 1130.795 kb on + strand, within HMPREF1058_RS14690 at 1130.800 kb on + strand, within HMPREF1058_RS14690 at 1130.822 kb on - strand, within HMPREF1058_RS14690 at 1130.824 kb on - strand, within HMPREF1058_RS14690 at 1130.888 kb on - strand, within HMPREF1058_RS14690 at 1130.888 kb on - strand, within HMPREF1058_RS14690 at 1130.929 kb on + strand, within HMPREF1058_RS14690 at 1130.946 kb on - strand, within HMPREF1058_RS14690 at 1130.968 kb on + strand, within HMPREF1058_RS14690 at 1130.974 kb on + strand, within HMPREF1058_RS14690 at 1130.975 kb on - strand, within HMPREF1058_RS14690 at 1130.975 kb on - strand, within HMPREF1058_RS14690 at 1130.981 kb on - strand, within HMPREF1058_RS14690 at 1130.981 kb on - strand, within HMPREF1058_RS14690 at 1131.002 kb on + strand, within HMPREF1058_RS14690 at 1131.134 kb on + strand, within HMPREF1058_RS14690 at 1131.160 kb on + strand, within HMPREF1058_RS14690 at 1131.161 kb on - strand, within HMPREF1058_RS14690 at 1131.174 kb on - strand, within HMPREF1058_RS14690 at 1131.250 kb on + strand at 1131.251 kb on - strand, within HMPREF1058_RS14690 at 1131.251 kb on - strand, within HMPREF1058_RS14690 at 1131.285 kb on - strand at 1131.290 kb on - strand, within HMPREF1058_RS14690 at 1131.309 kb on + strand, within HMPREF1058_RS14690 at 1131.389 kb on - strand at 1131.455 kb on + strand at 1131.456 kb on - strand at 1131.560 kb on + strand at 1131.561 kb on - strand at 1131.633 kb on - strand, within HMPREF1058_RS14685 at 1131.694 kb on + strand, within HMPREF1058_RS14685 at 1131.695 kb on - strand, within HMPREF1058_RS14685 at 1131.695 kb on - strand, within HMPREF1058_RS14685 at 1131.695 kb on - strand, within HMPREF1058_RS14685 at 1131.764 kb on + strand, within HMPREF1058_RS14685 at 1131.818 kb on + strand, within HMPREF1058_RS14685 at 1131.818 kb on + strand, within HMPREF1058_RS14685 at 1131.818 kb on + strand, within HMPREF1058_RS14685 at 1131.881 kb on + strand, within HMPREF1058_RS14685 at 1131.915 kb on - strand, within HMPREF1058_RS14685 at 1132.151 kb on + strand, within HMPREF1058_RS14685 at 1132.190 kb on + strand, within HMPREF1058_RS14685 at 1132.227 kb on - strand, within HMPREF1058_RS14685 at 1132.229 kb on + strand, within HMPREF1058_RS14685 at 1132.300 kb on - strand, within HMPREF1058_RS14685 at 1132.358 kb on + strand, within HMPREF1058_RS14685 at 1132.404 kb on + strand, within HMPREF1058_RS14685
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 1,130,214 + -0.6 1,130,215 - -1.1 1,130,229 - -2.6 1,130,312 - +0.5 1,130,354 - -0.0 1,130,385 - -1.3 1,130,394 - +1.5 1,130,402 + -1.5 1,130,403 - -0.0 1,130,403 - -0.8 1,130,403 - -1.2 1,130,423 - +0.3 1,130,510 - -2.4 1,130,569 + HMPREF1058_RS14690 0.10 +0.2 1,130,594 + HMPREF1058_RS14690 0.13 -1.6 1,130,619 + HMPREF1058_RS14690 0.15 -1.6 1,130,619 + HMPREF1058_RS14690 0.15 +1.7 1,130,622 + HMPREF1058_RS14690 0.16 -2.9 1,130,622 + HMPREF1058_RS14690 0.16 -2.5 1,130,626 - HMPREF1058_RS14690 0.16 -1.4 1,130,633 - HMPREF1058_RS14690 0.17 +1.6 1,130,633 - HMPREF1058_RS14690 0.17 -2.0 1,130,661 - HMPREF1058_RS14690 0.20 -1.2 1,130,704 + HMPREF1058_RS14690 0.24 -2.3 1,130,734 + HMPREF1058_RS14690 0.27 +0.3 1,130,752 + HMPREF1058_RS14690 0.29 +1.5 1,130,765 - HMPREF1058_RS14690 0.31 -0.8 1,130,765 - HMPREF1058_RS14690 0.31 -1.5 1,130,765 - HMPREF1058_RS14690 0.31 -2.2 1,130,788 + HMPREF1058_RS14690 0.33 +0.5 1,130,795 + HMPREF1058_RS14690 0.34 -1.2 1,130,800 + HMPREF1058_RS14690 0.34 -1.6 1,130,822 - HMPREF1058_RS14690 0.36 -3.7 1,130,824 - HMPREF1058_RS14690 0.37 -3.1 1,130,888 - HMPREF1058_RS14690 0.43 -2.8 1,130,888 - HMPREF1058_RS14690 0.43 -1.9 1,130,929 + HMPREF1058_RS14690 0.48 -2.1 1,130,946 - HMPREF1058_RS14690 0.49 -1.9 1,130,968 + HMPREF1058_RS14690 0.52 -1.4 1,130,974 + HMPREF1058_RS14690 0.52 -2.0 1,130,975 - HMPREF1058_RS14690 0.52 -0.3 1,130,975 - HMPREF1058_RS14690 0.52 -2.2 1,130,981 - HMPREF1058_RS14690 0.53 -1.6 1,130,981 - HMPREF1058_RS14690 0.53 +0.2 1,131,002 + HMPREF1058_RS14690 0.55 -1.5 1,131,134 + HMPREF1058_RS14690 0.69 -3.0 1,131,160 + HMPREF1058_RS14690 0.72 -0.8 1,131,161 - HMPREF1058_RS14690 0.72 -1.0 1,131,174 - HMPREF1058_RS14690 0.73 -1.5 1,131,250 + +0.5 1,131,251 - HMPREF1058_RS14690 0.81 -0.5 1,131,251 - HMPREF1058_RS14690 0.81 -1.0 1,131,285 - +0.5 1,131,290 - HMPREF1058_RS14690 0.85 -1.7 1,131,309 + HMPREF1058_RS14690 0.87 -0.7 1,131,389 - -2.1 1,131,455 + -1.1 1,131,456 - -0.3 1,131,560 + -1.0 1,131,561 - -0.5 1,131,633 - HMPREF1058_RS14685 0.12 +0.2 1,131,694 + HMPREF1058_RS14685 0.15 -1.1 1,131,695 - HMPREF1058_RS14685 0.15 -0.9 1,131,695 - HMPREF1058_RS14685 0.15 -0.0 1,131,695 - HMPREF1058_RS14685 0.15 -0.8 1,131,764 + HMPREF1058_RS14685 0.19 -1.7 1,131,818 + HMPREF1058_RS14685 0.22 -1.8 1,131,818 + HMPREF1058_RS14685 0.22 -2.0 1,131,818 + HMPREF1058_RS14685 0.22 -2.0 1,131,881 + HMPREF1058_RS14685 0.25 -2.9 1,131,915 - HMPREF1058_RS14685 0.27 -0.9 1,132,151 + HMPREF1058_RS14685 0.40 -1.4 1,132,190 + HMPREF1058_RS14685 0.42 +0.0 1,132,227 - HMPREF1058_RS14685 0.44 -0.3 1,132,229 + HMPREF1058_RS14685 0.44 -1.8 1,132,300 - HMPREF1058_RS14685 0.48 -0.3 1,132,358 + HMPREF1058_RS14685 0.51 -2.6 1,132,404 + HMPREF1058_RS14685 0.54 -1.6
Or see this region's nucleotide sequence