Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS12060 and HMPREF1058_RS12055 are separated by 191 nucleotides HMPREF1058_RS12055 and HMPREF1058_RS12050 are separated by 48 nucleotides HMPREF1058_RS12050 and HMPREF1058_RS0119610 are separated by 50 nucleotides
HMPREF1058_RS12060: HMPREF1058_RS12060 - outer membrane beta-barrel protein, at 210,957 to 213,236
_RS12060
HMPREF1058_RS12055: HMPREF1058_RS12055 - HAMP domain-containing histidine kinase, at 213,428 to 214,789
_RS12055
HMPREF1058_RS12050: HMPREF1058_RS12050 - response regulator transcription factor, at 214,838 to 215,506
_RS12050
HMPREF1058_RS0119610: HMPREF1058_RS0119610 - TonB-dependent receptor, at 215,557 to 217,956
_RS0119610
Position (kb)
213
214
215 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7 at 212.501 kb on - strand, within HMPREF1058_RS12060 at 212.510 kb on + strand, within HMPREF1058_RS12060 at 212.554 kb on + strand, within HMPREF1058_RS12060 at 212.591 kb on + strand, within HMPREF1058_RS12060 at 212.681 kb on + strand, within HMPREF1058_RS12060 at 212.682 kb on - strand, within HMPREF1058_RS12060 at 212.703 kb on - strand, within HMPREF1058_RS12060 at 212.748 kb on + strand, within HMPREF1058_RS12060 at 212.839 kb on + strand, within HMPREF1058_RS12060 at 212.839 kb on + strand, within HMPREF1058_RS12060 at 212.846 kb on + strand, within HMPREF1058_RS12060 at 212.847 kb on - strand, within HMPREF1058_RS12060 at 212.938 kb on + strand, within HMPREF1058_RS12060 at 212.938 kb on + strand, within HMPREF1058_RS12060 at 212.938 kb on + strand, within HMPREF1058_RS12060 at 212.939 kb on - strand, within HMPREF1058_RS12060 at 212.951 kb on + strand, within HMPREF1058_RS12060 at 213.009 kb on + strand at 213.010 kb on - strand at 213.016 kb on + strand at 213.135 kb on - strand at 213.213 kb on + strand at 213.214 kb on - strand at 213.237 kb on - strand at 213.237 kb on - strand at 213.254 kb on - strand at 213.281 kb on + strand at 213.330 kb on - strand at 213.418 kb on + strand at 213.423 kb on + strand at 213.423 kb on + strand at 213.424 kb on - strand at 213.444 kb on - strand at 213.444 kb on - strand at 213.444 kb on - strand at 213.447 kb on + strand at 213.451 kb on + strand at 213.451 kb on + strand at 213.452 kb on - strand at 213.452 kb on - strand at 213.454 kb on - strand at 213.469 kb on - strand at 213.476 kb on + strand at 213.480 kb on + strand at 213.480 kb on + strand at 213.481 kb on - strand at 213.481 kb on - strand at 213.602 kb on - strand, within HMPREF1058_RS12055 at 213.694 kb on - strand, within HMPREF1058_RS12055 at 213.721 kb on + strand, within HMPREF1058_RS12055 at 213.761 kb on - strand, within HMPREF1058_RS12055 at 213.762 kb on + strand, within HMPREF1058_RS12055 at 213.763 kb on - strand, within HMPREF1058_RS12055 at 213.763 kb on - strand, within HMPREF1058_RS12055 at 213.766 kb on + strand, within HMPREF1058_RS12055 at 213.766 kb on + strand, within HMPREF1058_RS12055 at 213.767 kb on - strand, within HMPREF1058_RS12055 at 213.767 kb on - strand, within HMPREF1058_RS12055 at 213.767 kb on - strand, within HMPREF1058_RS12055 at 213.851 kb on - strand, within HMPREF1058_RS12055 at 213.860 kb on + strand, within HMPREF1058_RS12055 at 213.861 kb on - strand, within HMPREF1058_RS12055 at 213.872 kb on - strand, within HMPREF1058_RS12055 at 213.899 kb on - strand, within HMPREF1058_RS12055 at 213.919 kb on + strand, within HMPREF1058_RS12055 at 213.919 kb on + strand, within HMPREF1058_RS12055 at 213.925 kb on - strand, within HMPREF1058_RS12055 at 213.942 kb on - strand, within HMPREF1058_RS12055 at 213.963 kb on + strand, within HMPREF1058_RS12055 at 213.964 kb on - strand, within HMPREF1058_RS12055 at 213.964 kb on - strand, within HMPREF1058_RS12055 at 214.015 kb on + strand, within HMPREF1058_RS12055 at 214.025 kb on - strand, within HMPREF1058_RS12055 at 214.030 kb on - strand, within HMPREF1058_RS12055 at 214.080 kb on + strand, within HMPREF1058_RS12055 at 214.156 kb on - strand, within HMPREF1058_RS12055 at 214.348 kb on - strand, within HMPREF1058_RS12055 at 214.351 kb on - strand, within HMPREF1058_RS12055 at 214.452 kb on + strand, within HMPREF1058_RS12055 at 214.452 kb on + strand, within HMPREF1058_RS12055 at 214.453 kb on - strand, within HMPREF1058_RS12055 at 214.453 kb on - strand at 214.461 kb on + strand, within HMPREF1058_RS12055 at 214.462 kb on - strand, within HMPREF1058_RS12055 at 214.463 kb on + strand, within HMPREF1058_RS12055 at 214.463 kb on + strand, within HMPREF1058_RS12055 at 214.464 kb on - strand, within HMPREF1058_RS12055 at 214.464 kb on - strand, within HMPREF1058_RS12055 at 214.464 kb on - strand, within HMPREF1058_RS12055 at 214.508 kb on + strand, within HMPREF1058_RS12055 at 214.620 kb on + strand, within HMPREF1058_RS12055 at 214.625 kb on - strand, within HMPREF1058_RS12055 at 214.681 kb on - strand at 214.686 kb on + strand at 214.700 kb on + strand at 214.700 kb on + strand at 214.701 kb on - strand at 214.703 kb on - strand at 214.705 kb on - strand at 214.707 kb on + strand at 214.708 kb on - strand at 214.832 kb on + strand at 214.833 kb on - strand at 214.833 kb on - strand at 214.833 kb on - strand at 214.850 kb on - strand at 214.876 kb on - strand at 214.892 kb on - strand at 214.926 kb on + strand, within HMPREF1058_RS12050 at 214.926 kb on + strand, within HMPREF1058_RS12050 at 214.993 kb on - strand, within HMPREF1058_RS12050 at 215.080 kb on - strand, within HMPREF1058_RS12050 at 215.160 kb on + strand, within HMPREF1058_RS12050 at 215.178 kb on - strand, within HMPREF1058_RS12050 at 215.178 kb on - strand, within HMPREF1058_RS12050 at 215.216 kb on - strand, within HMPREF1058_RS12050 at 215.217 kb on + strand, within HMPREF1058_RS12050 at 215.217 kb on + strand, within HMPREF1058_RS12050 at 215.218 kb on - strand, within HMPREF1058_RS12050 at 215.262 kb on - strand, within HMPREF1058_RS12050 at 215.326 kb on + strand, within HMPREF1058_RS12050 at 215.332 kb on + strand, within HMPREF1058_RS12050 at 215.411 kb on + strand, within HMPREF1058_RS12050 at 215.419 kb on - strand, within HMPREF1058_RS12050 at 215.454 kb on + strand at 215.455 kb on - strand at 215.455 kb on - strand at 215.455 kb on - strand at 215.486 kb on - strand at 215.486 kb on - strand at 215.486 kb on - strand at 215.523 kb on + strand at 215.527 kb on + strand at 215.536 kb on - strand at 215.628 kb on - strand at 215.685 kb on - strand at 215.698 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 212,501 - HMPREF1058_RS12060 0.68 +0.3 212,510 + HMPREF1058_RS12060 0.68 -0.7 212,554 + HMPREF1058_RS12060 0.70 -0.8 212,591 + HMPREF1058_RS12060 0.72 -0.7 212,681 + HMPREF1058_RS12060 0.76 -0.5 212,682 - HMPREF1058_RS12060 0.76 -1.0 212,703 - HMPREF1058_RS12060 0.77 -0.7 212,748 + HMPREF1058_RS12060 0.79 -0.6 212,839 + HMPREF1058_RS12060 0.83 -1.7 212,839 + HMPREF1058_RS12060 0.83 -0.8 212,846 + HMPREF1058_RS12060 0.83 -1.2 212,847 - HMPREF1058_RS12060 0.83 +1.9 212,938 + HMPREF1058_RS12060 0.87 -0.0 212,938 + HMPREF1058_RS12060 0.87 -1.4 212,938 + HMPREF1058_RS12060 0.87 -1.7 212,939 - HMPREF1058_RS12060 0.87 +1.4 212,951 + HMPREF1058_RS12060 0.87 -1.4 213,009 + +0.1 213,010 - -0.7 213,016 + +1.0 213,135 - +0.2 213,213 + +1.4 213,214 - -0.3 213,237 - -0.3 213,237 - -1.9 213,254 - +0.0 213,281 + +2.3 213,330 - -1.2 213,418 + -0.2 213,423 + -0.6 213,423 + -1.0 213,424 - -1.0 213,444 - +0.1 213,444 - -1.3 213,444 - +1.2 213,447 + -1.1 213,451 + +1.6 213,451 + -0.5 213,452 - -0.1 213,452 - -0.0 213,454 - +1.8 213,469 - -0.9 213,476 + -1.2 213,480 + -0.9 213,480 + +0.6 213,481 - -1.2 213,481 - -0.2 213,602 - HMPREF1058_RS12055 0.13 +1.4 213,694 - HMPREF1058_RS12055 0.20 -0.9 213,721 + HMPREF1058_RS12055 0.22 +1.1 213,761 - HMPREF1058_RS12055 0.24 +1.2 213,762 + HMPREF1058_RS12055 0.25 -1.9 213,763 - HMPREF1058_RS12055 0.25 +1.3 213,763 - HMPREF1058_RS12055 0.25 -0.8 213,766 + HMPREF1058_RS12055 0.25 -0.8 213,766 + HMPREF1058_RS12055 0.25 -1.1 213,767 - HMPREF1058_RS12055 0.25 -0.7 213,767 - HMPREF1058_RS12055 0.25 +0.4 213,767 - HMPREF1058_RS12055 0.25 -0.6 213,851 - HMPREF1058_RS12055 0.31 -0.3 213,860 + HMPREF1058_RS12055 0.32 +1.1 213,861 - HMPREF1058_RS12055 0.32 -0.8 213,872 - HMPREF1058_RS12055 0.33 +3.1 213,899 - HMPREF1058_RS12055 0.35 +0.8 213,919 + HMPREF1058_RS12055 0.36 -1.3 213,919 + HMPREF1058_RS12055 0.36 -0.0 213,925 - HMPREF1058_RS12055 0.36 +1.1 213,942 - HMPREF1058_RS12055 0.38 +0.8 213,963 + HMPREF1058_RS12055 0.39 -0.3 213,964 - HMPREF1058_RS12055 0.39 -0.5 213,964 - HMPREF1058_RS12055 0.39 -0.5 214,015 + HMPREF1058_RS12055 0.43 -0.0 214,025 - HMPREF1058_RS12055 0.44 +0.3 214,030 - HMPREF1058_RS12055 0.44 -1.5 214,080 + HMPREF1058_RS12055 0.48 +0.0 214,156 - HMPREF1058_RS12055 0.53 -1.6 214,348 - HMPREF1058_RS12055 0.68 -0.6 214,351 - HMPREF1058_RS12055 0.68 -0.5 214,452 + HMPREF1058_RS12055 0.75 +0.4 214,452 + HMPREF1058_RS12055 0.75 -2.1 214,453 - HMPREF1058_RS12055 0.75 -0.4 214,453 - -0.9 214,461 + HMPREF1058_RS12055 0.76 -0.2 214,462 - HMPREF1058_RS12055 0.76 -0.7 214,463 + HMPREF1058_RS12055 0.76 +0.6 214,463 + HMPREF1058_RS12055 0.76 -1.1 214,464 - HMPREF1058_RS12055 0.76 +0.3 214,464 - HMPREF1058_RS12055 0.76 +1.4 214,464 - HMPREF1058_RS12055 0.76 +0.1 214,508 + HMPREF1058_RS12055 0.79 +1.1 214,620 + HMPREF1058_RS12055 0.88 -0.7 214,625 - HMPREF1058_RS12055 0.88 +1.4 214,681 - -2.0 214,686 + -1.1 214,700 + -0.5 214,700 + +0.4 214,701 - -1.5 214,703 - +1.2 214,705 - -2.5 214,707 + +1.0 214,708 - -0.7 214,832 + +0.9 214,833 - -0.1 214,833 - +0.1 214,833 - +7.5 214,850 - +3.4 214,876 - -0.3 214,892 - -1.5 214,926 + HMPREF1058_RS12050 0.13 -0.3 214,926 + HMPREF1058_RS12050 0.13 +0.2 214,993 - HMPREF1058_RS12050 0.23 -0.2 215,080 - HMPREF1058_RS12050 0.36 +0.1 215,160 + HMPREF1058_RS12050 0.48 +1.3 215,178 - HMPREF1058_RS12050 0.51 +0.5 215,178 - HMPREF1058_RS12050 0.51 -0.2 215,216 - HMPREF1058_RS12050 0.57 -0.1 215,217 + HMPREF1058_RS12050 0.57 -2.0 215,217 + HMPREF1058_RS12050 0.57 +0.1 215,218 - HMPREF1058_RS12050 0.57 +1.3 215,262 - HMPREF1058_RS12050 0.63 +0.3 215,326 + HMPREF1058_RS12050 0.73 +1.0 215,332 + HMPREF1058_RS12050 0.74 -0.7 215,411 + HMPREF1058_RS12050 0.86 +0.8 215,419 - HMPREF1058_RS12050 0.87 +1.8 215,454 + +0.3 215,455 - -0.9 215,455 - -0.8 215,455 - -2.3 215,486 - -0.6 215,486 - -0.1 215,486 - -0.4 215,523 + -0.7 215,527 + -1.7 215,536 - -0.4 215,628 - -0.6 215,685 - -0.1 215,698 + +0.1
Or see this region's nucleotide sequence