Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS09165 and HMPREF1058_RS09160 are separated by 10 nucleotides HMPREF1058_RS09160 and HMPREF1058_RS09155 are separated by 122 nucleotides HMPREF1058_RS09155 and HMPREF1058_RS09150 are separated by 128 nucleotides
HMPREF1058_RS09165: HMPREF1058_RS09165 - SDR family NAD(P)-dependent oxidoreductase, at 921,969 to 922,697
_RS09165
HMPREF1058_RS09160: HMPREF1058_RS09160 - O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase, at 922,708 to 923,985
_RS09160
HMPREF1058_RS09155: HMPREF1058_RS09155 - acyl-CoA thioesterase, at 924,108 to 924,557
_RS09155
HMPREF1058_RS09150: HMPREF1058_RS09150 - RagB/SusD family nutrient uptake outer membrane protein, at 924,686 to 926,251
_RS09150
Position (kb)
922
923
924 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 921.748 kb on + strand at 921.757 kb on - strand at 922.053 kb on + strand, within HMPREF1058_RS09165 at 922.089 kb on + strand, within HMPREF1058_RS09165 at 922.197 kb on + strand, within HMPREF1058_RS09165 at 922.197 kb on + strand, within HMPREF1058_RS09165 at 922.237 kb on + strand, within HMPREF1058_RS09165 at 922.237 kb on + strand, within HMPREF1058_RS09165 at 922.323 kb on - strand, within HMPREF1058_RS09165 at 922.323 kb on - strand at 922.348 kb on + strand, within HMPREF1058_RS09165 at 922.348 kb on + strand, within HMPREF1058_RS09165 at 922.349 kb on - strand, within HMPREF1058_RS09165 at 922.413 kb on + strand, within HMPREF1058_RS09165 at 922.413 kb on + strand, within HMPREF1058_RS09165 at 922.551 kb on + strand, within HMPREF1058_RS09165 at 922.551 kb on + strand, within HMPREF1058_RS09165 at 922.551 kb on + strand, within HMPREF1058_RS09165 at 922.551 kb on + strand, within HMPREF1058_RS09165 at 922.552 kb on - strand, within HMPREF1058_RS09165 at 922.552 kb on - strand, within HMPREF1058_RS09165 at 922.552 kb on - strand, within HMPREF1058_RS09165 at 922.552 kb on - strand, within HMPREF1058_RS09165 at 922.552 kb on - strand, within HMPREF1058_RS09165 at 922.683 kb on - strand at 922.748 kb on - strand at 922.860 kb on + strand, within HMPREF1058_RS09160 at 922.860 kb on + strand, within HMPREF1058_RS09160 at 922.860 kb on + strand, within HMPREF1058_RS09160 at 922.860 kb on + strand, within HMPREF1058_RS09160 at 922.982 kb on + strand, within HMPREF1058_RS09160 at 922.982 kb on + strand, within HMPREF1058_RS09160 at 922.983 kb on - strand, within HMPREF1058_RS09160 at 923.028 kb on - strand, within HMPREF1058_RS09160 at 923.233 kb on - strand, within HMPREF1058_RS09160 at 923.234 kb on + strand, within HMPREF1058_RS09160 at 923.360 kb on + strand, within HMPREF1058_RS09160 at 923.472 kb on + strand, within HMPREF1058_RS09160 at 923.566 kb on + strand, within HMPREF1058_RS09160 at 923.650 kb on - strand, within HMPREF1058_RS09160 at 923.675 kb on - strand, within HMPREF1058_RS09160 at 923.701 kb on - strand, within HMPREF1058_RS09160 at 923.822 kb on + strand, within HMPREF1058_RS09160 at 923.823 kb on - strand, within HMPREF1058_RS09160 at 923.879 kb on + strand at 923.973 kb on - strand at 923.973 kb on - strand at 923.991 kb on + strand at 924.006 kb on + strand at 924.010 kb on + strand at 924.011 kb on - strand at 924.034 kb on + strand at 924.104 kb on - strand at 924.134 kb on + strand at 924.135 kb on - strand at 924.138 kb on - strand at 924.182 kb on + strand, within HMPREF1058_RS09155 at 924.183 kb on - strand, within HMPREF1058_RS09155 at 924.227 kb on + strand, within HMPREF1058_RS09155 at 924.231 kb on + strand, within HMPREF1058_RS09155 at 924.231 kb on + strand, within HMPREF1058_RS09155 at 924.235 kb on + strand, within HMPREF1058_RS09155 at 924.236 kb on - strand, within HMPREF1058_RS09155 at 924.236 kb on - strand, within HMPREF1058_RS09155 at 924.236 kb on - strand, within HMPREF1058_RS09155 at 924.245 kb on - strand, within HMPREF1058_RS09155 at 924.248 kb on - strand, within HMPREF1058_RS09155 at 924.248 kb on - strand, within HMPREF1058_RS09155 at 924.341 kb on - strand, within HMPREF1058_RS09155 at 924.445 kb on + strand, within HMPREF1058_RS09155 at 924.446 kb on - strand, within HMPREF1058_RS09155 at 924.446 kb on - strand, within HMPREF1058_RS09155 at 924.446 kb on - strand, within HMPREF1058_RS09155 at 924.492 kb on + strand, within HMPREF1058_RS09155 at 924.493 kb on - strand, within HMPREF1058_RS09155 at 924.555 kb on + strand at 924.556 kb on - strand at 924.593 kb on + strand at 924.595 kb on + strand at 924.595 kb on + strand at 924.601 kb on + strand at 924.616 kb on + strand at 924.643 kb on - strand at 924.748 kb on + strand at 924.766 kb on + strand at 924.807 kb on + strand at 924.863 kb on + strand, within HMPREF1058_RS09150 at 924.939 kb on + strand, within HMPREF1058_RS09150 at 924.981 kb on + strand, within HMPREF1058_RS09150
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 921,748 + -0.7 921,757 - -0.5 922,053 + HMPREF1058_RS09165 0.12 -1.2 922,089 + HMPREF1058_RS09165 0.16 -0.2 922,197 + HMPREF1058_RS09165 0.31 +0.1 922,197 + HMPREF1058_RS09165 0.31 +0.7 922,237 + HMPREF1058_RS09165 0.37 +1.6 922,237 + HMPREF1058_RS09165 0.37 +1.9 922,323 - HMPREF1058_RS09165 0.49 -2.0 922,323 - +0.0 922,348 + HMPREF1058_RS09165 0.52 -2.1 922,348 + HMPREF1058_RS09165 0.52 +0.6 922,349 - HMPREF1058_RS09165 0.52 +2.7 922,413 + HMPREF1058_RS09165 0.61 -0.5 922,413 + HMPREF1058_RS09165 0.61 -0.7 922,551 + HMPREF1058_RS09165 0.80 -0.7 922,551 + HMPREF1058_RS09165 0.80 +1.4 922,551 + HMPREF1058_RS09165 0.80 +0.4 922,551 + HMPREF1058_RS09165 0.80 -0.4 922,552 - HMPREF1058_RS09165 0.80 -0.3 922,552 - HMPREF1058_RS09165 0.80 -0.5 922,552 - HMPREF1058_RS09165 0.80 -0.5 922,552 - HMPREF1058_RS09165 0.80 +0.5 922,552 - HMPREF1058_RS09165 0.80 +0.2 922,683 - +0.0 922,748 - +1.6 922,860 + HMPREF1058_RS09160 0.12 +0.7 922,860 + HMPREF1058_RS09160 0.12 +0.0 922,860 + HMPREF1058_RS09160 0.12 +1.6 922,860 + HMPREF1058_RS09160 0.12 +0.3 922,982 + HMPREF1058_RS09160 0.21 +0.5 922,982 + HMPREF1058_RS09160 0.21 +0.6 922,983 - HMPREF1058_RS09160 0.22 +1.9 923,028 - HMPREF1058_RS09160 0.25 +0.5 923,233 - HMPREF1058_RS09160 0.41 +1.4 923,234 + HMPREF1058_RS09160 0.41 -0.5 923,360 + HMPREF1058_RS09160 0.51 +2.1 923,472 + HMPREF1058_RS09160 0.60 +1.0 923,566 + HMPREF1058_RS09160 0.67 +1.1 923,650 - HMPREF1058_RS09160 0.74 +0.8 923,675 - HMPREF1058_RS09160 0.76 +1.2 923,701 - HMPREF1058_RS09160 0.78 +2.2 923,822 + HMPREF1058_RS09160 0.87 -1.3 923,823 - HMPREF1058_RS09160 0.87 +0.9 923,879 + +1.4 923,973 - +1.1 923,973 - -0.4 923,991 + +5.3 924,006 + +1.6 924,010 + +1.1 924,011 - -0.1 924,034 + +0.4 924,104 - -3.1 924,134 + +1.8 924,135 - -0.5 924,138 - -1.4 924,182 + HMPREF1058_RS09155 0.16 -1.4 924,183 - HMPREF1058_RS09155 0.17 -1.6 924,227 + HMPREF1058_RS09155 0.26 +0.8 924,231 + HMPREF1058_RS09155 0.27 -1.1 924,231 + HMPREF1058_RS09155 0.27 +0.5 924,235 + HMPREF1058_RS09155 0.28 +1.3 924,236 - HMPREF1058_RS09155 0.28 +0.8 924,236 - HMPREF1058_RS09155 0.28 -0.2 924,236 - HMPREF1058_RS09155 0.28 -0.6 924,245 - HMPREF1058_RS09155 0.30 -1.3 924,248 - HMPREF1058_RS09155 0.31 -1.1 924,248 - HMPREF1058_RS09155 0.31 -1.8 924,341 - HMPREF1058_RS09155 0.52 -2.8 924,445 + HMPREF1058_RS09155 0.75 +0.7 924,446 - HMPREF1058_RS09155 0.75 -0.6 924,446 - HMPREF1058_RS09155 0.75 +1.5 924,446 - HMPREF1058_RS09155 0.75 +2.5 924,492 + HMPREF1058_RS09155 0.85 -1.0 924,493 - HMPREF1058_RS09155 0.86 -2.7 924,555 + -0.8 924,556 - -0.8 924,593 + +0.5 924,595 + +0.1 924,595 + +1.5 924,601 + -1.6 924,616 + -0.1 924,643 - +0.3 924,748 + +3.1 924,766 + +0.8 924,807 + +0.2 924,863 + HMPREF1058_RS09150 0.11 -0.1 924,939 + HMPREF1058_RS09150 0.16 +2.4 924,981 + HMPREF1058_RS09150 0.19 -1.5
Or see this region's nucleotide sequence