Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS07550 and HMPREF1058_RS07545 are separated by 278 nucleotides HMPREF1058_RS07545 and HMPREF1058_RS07540 are separated by 21 nucleotides HMPREF1058_RS07540 and HMPREF1058_RS07535 are separated by 14 nucleotides HMPREF1058_RS07535 and HMPREF1058_RS07530 are separated by 364 nucleotides
HMPREF1058_RS07550: HMPREF1058_RS07550 - alpha/beta hydrolase, at 192,978 to 194,033
_RS07550
HMPREF1058_RS07545: HMPREF1058_RS07545 - hypothetical protein, at 194,312 to 194,491
_RS07545
HMPREF1058_RS07540: HMPREF1058_RS07540 - acetyltransferase, at 194,513 to 195,088
_RS07540
HMPREF1058_RS07535: HMPREF1058_RS07535 - hypothetical protein, at 195,103 to 195,390
_RS07535
HMPREF1058_RS07530: HMPREF1058_RS07530 - alpha/beta hydrolase, at 195,755 to 196,624
_RS07530
Position (kb) 194
195
196 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 193.741 kb on + strand, within HMPREF1058_RS07550 at 193.764 kb on + strand, within HMPREF1058_RS07550 at 194.159 kb on - strand at 194.301 kb on + strand at 194.312 kb on - strand at 194.373 kb on - strand, within HMPREF1058_RS07545 at 194.448 kb on - strand, within HMPREF1058_RS07545 at 194.490 kb on - strand at 194.666 kb on + strand, within HMPREF1058_RS07540 at 194.666 kb on + strand, within HMPREF1058_RS07540 at 194.854 kb on + strand, within HMPREF1058_RS07540 at 194.855 kb on - strand, within HMPREF1058_RS07540 at 195.080 kb on + strand at 195.081 kb on - strand at 195.088 kb on + strand at 195.089 kb on - strand at 195.107 kb on - strand at 195.125 kb on + strand at 195.200 kb on - strand, within HMPREF1058_RS07535 at 195.290 kb on + strand, within HMPREF1058_RS07535 at 195.291 kb on - strand, within HMPREF1058_RS07535 at 195.291 kb on - strand, within HMPREF1058_RS07535 at 195.346 kb on + strand, within HMPREF1058_RS07535 at 195.356 kb on - strand, within HMPREF1058_RS07535 at 195.381 kb on + strand at 195.381 kb on + strand at 195.491 kb on + strand at 195.521 kb on - strand at 195.574 kb on + strand at 195.575 kb on - strand at 195.578 kb on + strand at 195.619 kb on - strand at 195.630 kb on - strand at 195.683 kb on + strand at 195.689 kb on - strand at 195.716 kb on - strand at 195.716 kb on - strand at 195.720 kb on - strand at 195.754 kb on - strand at 195.979 kb on - strand, within HMPREF1058_RS07530 at 196.067 kb on + strand, within HMPREF1058_RS07530 at 196.073 kb on + strand, within HMPREF1058_RS07530 at 196.074 kb on - strand, within HMPREF1058_RS07530 at 196.076 kb on - strand, within HMPREF1058_RS07530
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 193,741 + HMPREF1058_RS07550 0.72 +1.4 193,764 + HMPREF1058_RS07550 0.74 -0.8 194,159 - +2.3 194,301 + -1.3 194,312 - +0.7 194,373 - HMPREF1058_RS07545 0.34 +0.2 194,448 - HMPREF1058_RS07545 0.76 -0.6 194,490 - -0.2 194,666 + HMPREF1058_RS07540 0.27 -2.0 194,666 + HMPREF1058_RS07540 0.27 +0.0 194,854 + HMPREF1058_RS07540 0.59 -1.6 194,855 - HMPREF1058_RS07540 0.59 +3.0 195,080 + -1.0 195,081 - +1.3 195,088 + -0.7 195,089 - +3.2 195,107 - -0.8 195,125 + +0.8 195,200 - HMPREF1058_RS07535 0.34 +1.8 195,290 + HMPREF1058_RS07535 0.65 -1.3 195,291 - HMPREF1058_RS07535 0.65 +0.3 195,291 - HMPREF1058_RS07535 0.65 -2.7 195,346 + HMPREF1058_RS07535 0.84 -0.3 195,356 - HMPREF1058_RS07535 0.88 -1.3 195,381 + -0.4 195,381 + -0.8 195,491 + -1.4 195,521 - +1.0 195,574 + -1.8 195,575 - -0.6 195,578 + +1.6 195,619 - +0.3 195,630 - -1.9 195,683 + +1.0 195,689 - -0.2 195,716 - +3.3 195,716 - +0.4 195,720 - +1.2 195,754 - -2.0 195,979 - HMPREF1058_RS07530 0.26 +0.3 196,067 + HMPREF1058_RS07530 0.36 +0.3 196,073 + HMPREF1058_RS07530 0.37 +0.0 196,074 - HMPREF1058_RS07530 0.37 -0.6 196,076 - HMPREF1058_RS07530 0.37 -0.5
Or see this region's nucleotide sequence