Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS06700 and HMPREF1058_RS06695 are separated by 7 nucleotides HMPREF1058_RS06695 and HMPREF1058_RS06690 overlap by 4 nucleotides HMPREF1058_RS06690 and HMPREF1058_RS06685 overlap by 20 nucleotides
HMPREF1058_RS06700: HMPREF1058_RS06700 - glycosyltransferase family 2 protein, at 415,076 to 416,236
_RS06700
HMPREF1058_RS06695: HMPREF1058_RS06695 - glycosyltransferase, at 416,244 to 417,368
_RS06695
HMPREF1058_RS06690: HMPREF1058_RS06690 - glycosyltransferase family 2 protein, at 417,365 to 418,270
_RS06690
HMPREF1058_RS06685: HMPREF1058_RS06685 - O-antigen ligase family protein, at 418,251 to 419,711
_RS06685
Position (kb)
416
417
418 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 415.402 kb on + strand, within HMPREF1058_RS06700 at 415.514 kb on - strand, within HMPREF1058_RS06700 at 415.515 kb on + strand, within HMPREF1058_RS06700 at 415.515 kb on + strand, within HMPREF1058_RS06700 at 415.516 kb on - strand, within HMPREF1058_RS06700 at 415.516 kb on - strand, within HMPREF1058_RS06700 at 415.516 kb on - strand, within HMPREF1058_RS06700 at 415.770 kb on - strand, within HMPREF1058_RS06700 at 415.770 kb on - strand, within HMPREF1058_RS06700 at 415.839 kb on - strand, within HMPREF1058_RS06700 at 415.854 kb on + strand, within HMPREF1058_RS06700 at 415.929 kb on - strand, within HMPREF1058_RS06700 at 415.995 kb on - strand, within HMPREF1058_RS06700 at 416.092 kb on + strand, within HMPREF1058_RS06700 at 416.165 kb on + strand at 416.165 kb on + strand at 416.166 kb on - strand at 416.166 kb on - strand at 416.167 kb on + strand at 416.168 kb on - strand at 416.169 kb on + strand at 416.194 kb on + strand at 416.194 kb on + strand at 416.195 kb on - strand at 416.196 kb on + strand at 416.196 kb on + strand at 416.196 kb on + strand at 416.197 kb on - strand at 416.197 kb on - strand at 416.199 kb on - strand at 416.203 kb on + strand at 416.204 kb on - strand at 416.204 kb on - strand at 416.204 kb on - strand at 416.216 kb on - strand at 416.219 kb on + strand at 416.220 kb on - strand at 416.338 kb on + strand at 416.422 kb on - strand, within HMPREF1058_RS06695 at 416.454 kb on + strand, within HMPREF1058_RS06695 at 416.566 kb on + strand, within HMPREF1058_RS06695 at 416.588 kb on + strand, within HMPREF1058_RS06695 at 416.588 kb on + strand, within HMPREF1058_RS06695 at 416.588 kb on + strand, within HMPREF1058_RS06695 at 416.589 kb on - strand, within HMPREF1058_RS06695 at 416.634 kb on + strand, within HMPREF1058_RS06695 at 416.634 kb on + strand, within HMPREF1058_RS06695 at 416.634 kb on + strand, within HMPREF1058_RS06695 at 416.634 kb on + strand, within HMPREF1058_RS06695 at 416.635 kb on - strand, within HMPREF1058_RS06695 at 416.635 kb on - strand, within HMPREF1058_RS06695 at 416.635 kb on - strand, within HMPREF1058_RS06695 at 416.635 kb on - strand, within HMPREF1058_RS06695 at 416.635 kb on - strand, within HMPREF1058_RS06695 at 416.635 kb on - strand, within HMPREF1058_RS06695 at 416.643 kb on - strand, within HMPREF1058_RS06695 at 416.646 kb on - strand, within HMPREF1058_RS06695 at 416.650 kb on - strand, within HMPREF1058_RS06695 at 416.663 kb on - strand, within HMPREF1058_RS06695 at 416.664 kb on + strand, within HMPREF1058_RS06695 at 416.665 kb on - strand, within HMPREF1058_RS06695 at 416.920 kb on + strand, within HMPREF1058_RS06695 at 416.921 kb on - strand, within HMPREF1058_RS06695 at 417.152 kb on + strand, within HMPREF1058_RS06695 at 417.188 kb on - strand, within HMPREF1058_RS06695 at 417.192 kb on + strand, within HMPREF1058_RS06695 at 417.244 kb on - strand, within HMPREF1058_RS06695 at 417.318 kb on - strand at 417.340 kb on - strand at 417.349 kb on - strand at 417.357 kb on + strand at 417.480 kb on - strand, within HMPREF1058_RS06690 at 417.545 kb on + strand, within HMPREF1058_RS06690 at 417.545 kb on + strand, within HMPREF1058_RS06690 at 417.546 kb on - strand, within HMPREF1058_RS06690 at 417.546 kb on - strand, within HMPREF1058_RS06690 at 417.549 kb on - strand at 417.574 kb on - strand, within HMPREF1058_RS06690 at 417.644 kb on + strand, within HMPREF1058_RS06690 at 417.669 kb on + strand, within HMPREF1058_RS06690 at 417.670 kb on - strand, within HMPREF1058_RS06690 at 417.697 kb on - strand, within HMPREF1058_RS06690 at 417.724 kb on + strand, within HMPREF1058_RS06690 at 417.737 kb on + strand, within HMPREF1058_RS06690 at 417.806 kb on - strand, within HMPREF1058_RS06690 at 417.878 kb on + strand, within HMPREF1058_RS06690 at 417.954 kb on - strand, within HMPREF1058_RS06690 at 417.982 kb on + strand, within HMPREF1058_RS06690 at 417.983 kb on - strand, within HMPREF1058_RS06690 at 418.031 kb on + strand, within HMPREF1058_RS06690 at 418.046 kb on - strand, within HMPREF1058_RS06690 at 418.080 kb on - strand, within HMPREF1058_RS06690 at 418.161 kb on + strand, within HMPREF1058_RS06690 at 418.272 kb on + strand at 418.272 kb on + strand at 418.330 kb on + strand at 418.360 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 415,402 + HMPREF1058_RS06700 0.28 +0.7 415,514 - HMPREF1058_RS06700 0.38 +0.2 415,515 + HMPREF1058_RS06700 0.38 +0.5 415,515 + HMPREF1058_RS06700 0.38 -1.2 415,516 - HMPREF1058_RS06700 0.38 +2.6 415,516 - HMPREF1058_RS06700 0.38 +1.1 415,516 - HMPREF1058_RS06700 0.38 +0.3 415,770 - HMPREF1058_RS06700 0.60 +2.2 415,770 - HMPREF1058_RS06700 0.60 +1.0 415,839 - HMPREF1058_RS06700 0.66 +1.3 415,854 + HMPREF1058_RS06700 0.67 -0.9 415,929 - HMPREF1058_RS06700 0.73 +2.3 415,995 - HMPREF1058_RS06700 0.79 +0.6 416,092 + HMPREF1058_RS06700 0.88 -2.0 416,165 + -0.7 416,165 + +0.8 416,166 - -0.3 416,166 - +2.1 416,167 + +3.5 416,168 - -1.7 416,169 + -1.3 416,194 + -1.8 416,194 + +1.0 416,195 - +0.4 416,196 + +1.4 416,196 + -0.7 416,196 + -1.0 416,197 - +1.1 416,197 - -1.1 416,199 - -0.9 416,203 + -1.5 416,204 - -0.9 416,204 - +0.2 416,204 - -0.3 416,216 - -2.0 416,219 + +0.1 416,220 - +0.2 416,338 + -2.6 416,422 - HMPREF1058_RS06695 0.16 +1.0 416,454 + HMPREF1058_RS06695 0.19 -1.0 416,566 + HMPREF1058_RS06695 0.29 +2.6 416,588 + HMPREF1058_RS06695 0.31 -0.6 416,588 + HMPREF1058_RS06695 0.31 +0.9 416,588 + HMPREF1058_RS06695 0.31 -0.1 416,589 - HMPREF1058_RS06695 0.31 +1.5 416,634 + HMPREF1058_RS06695 0.35 +1.8 416,634 + HMPREF1058_RS06695 0.35 -0.8 416,634 + HMPREF1058_RS06695 0.35 -2.5 416,634 + HMPREF1058_RS06695 0.35 -0.3 416,635 - HMPREF1058_RS06695 0.35 +1.2 416,635 - HMPREF1058_RS06695 0.35 +1.2 416,635 - HMPREF1058_RS06695 0.35 -0.6 416,635 - HMPREF1058_RS06695 0.35 +0.6 416,635 - HMPREF1058_RS06695 0.35 +0.8 416,635 - HMPREF1058_RS06695 0.35 +0.2 416,643 - HMPREF1058_RS06695 0.35 +2.5 416,646 - HMPREF1058_RS06695 0.36 +2.4 416,650 - HMPREF1058_RS06695 0.36 +5.9 416,663 - HMPREF1058_RS06695 0.37 +0.2 416,664 + HMPREF1058_RS06695 0.37 +2.1 416,665 - HMPREF1058_RS06695 0.37 +2.1 416,920 + HMPREF1058_RS06695 0.60 +4.3 416,921 - HMPREF1058_RS06695 0.60 -1.5 417,152 + HMPREF1058_RS06695 0.81 +0.5 417,188 - HMPREF1058_RS06695 0.84 +3.3 417,192 + HMPREF1058_RS06695 0.84 -0.2 417,244 - HMPREF1058_RS06695 0.89 +4.0 417,318 - +1.6 417,340 - +0.8 417,349 - +0.5 417,357 + -0.7 417,480 - HMPREF1058_RS06690 0.13 +0.0 417,545 + HMPREF1058_RS06690 0.20 -2.3 417,545 + HMPREF1058_RS06690 0.20 -0.9 417,546 - HMPREF1058_RS06690 0.20 -0.5 417,546 - HMPREF1058_RS06690 0.20 -0.5 417,549 - +2.5 417,574 - HMPREF1058_RS06690 0.23 -0.1 417,644 + HMPREF1058_RS06690 0.31 +1.0 417,669 + HMPREF1058_RS06690 0.34 +0.2 417,670 - HMPREF1058_RS06690 0.34 -0.9 417,697 - HMPREF1058_RS06690 0.37 +1.7 417,724 + HMPREF1058_RS06690 0.40 -0.7 417,737 + HMPREF1058_RS06690 0.41 +3.3 417,806 - HMPREF1058_RS06690 0.49 +2.4 417,878 + HMPREF1058_RS06690 0.57 +2.1 417,954 - HMPREF1058_RS06690 0.65 +1.5 417,982 + HMPREF1058_RS06690 0.68 +0.1 417,983 - HMPREF1058_RS06690 0.68 -2.5 418,031 + HMPREF1058_RS06690 0.74 -1.3 418,046 - HMPREF1058_RS06690 0.75 +0.4 418,080 - HMPREF1058_RS06690 0.79 -1.4 418,161 + HMPREF1058_RS06690 0.88 +1.9 418,272 + +3.1 418,272 + +0.5 418,330 + +0.5 418,360 + -0.1
Or see this region's nucleotide sequence