Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS06245 and HMPREF1058_RS06240 are separated by 13 nucleotides HMPREF1058_RS06240 and HMPREF1058_RS06235 are separated by 33 nucleotides HMPREF1058_RS06235 and HMPREF1058_RS06230 are separated by 95 nucleotides
HMPREF1058_RS06245: HMPREF1058_RS06245 - dihydroorotate dehydrogenase-like protein, at 527,443 to 528,417
_RS06245
HMPREF1058_RS06240: HMPREF1058_RS06240 - YggS family pyridoxal phosphate-dependent enzyme, at 528,431 to 529,099
_RS06240
HMPREF1058_RS06235: HMPREF1058_RS06235 - DUF4494 domain-containing protein, at 529,133 to 529,615
_RS06235
HMPREF1058_RS06230: HMPREF1058_RS06230 - fucose isomerase, at 529,711 to 530,946
_RS06230
Position (kb)
528
529
530 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 527.479 kb on - strand at 527.479 kb on - strand at 527.589 kb on + strand, within HMPREF1058_RS06245 at 527.589 kb on + strand, within HMPREF1058_RS06245 at 527.590 kb on - strand, within HMPREF1058_RS06245 at 527.802 kb on - strand, within HMPREF1058_RS06245 at 527.802 kb on - strand, within HMPREF1058_RS06245 at 527.835 kb on - strand, within HMPREF1058_RS06245 at 527.965 kb on + strand, within HMPREF1058_RS06245 at 528.000 kb on - strand, within HMPREF1058_RS06245 at 528.168 kb on + strand, within HMPREF1058_RS06245 at 528.169 kb on - strand, within HMPREF1058_RS06245 at 528.193 kb on + strand, within HMPREF1058_RS06245 at 528.194 kb on - strand, within HMPREF1058_RS06245 at 528.194 kb on - strand, within HMPREF1058_RS06245 at 528.197 kb on + strand, within HMPREF1058_RS06245 at 528.197 kb on + strand at 528.197 kb on + strand, within HMPREF1058_RS06245 at 528.197 kb on + strand, within HMPREF1058_RS06245 at 528.197 kb on + strand, within HMPREF1058_RS06245 at 528.198 kb on - strand, within HMPREF1058_RS06245 at 528.198 kb on - strand, within HMPREF1058_RS06245 at 528.198 kb on - strand, within HMPREF1058_RS06245 at 528.198 kb on - strand, within HMPREF1058_RS06245 at 528.198 kb on - strand, within HMPREF1058_RS06245 at 528.198 kb on - strand, within HMPREF1058_RS06245 at 528.209 kb on + strand, within HMPREF1058_RS06245 at 528.233 kb on + strand, within HMPREF1058_RS06245 at 528.234 kb on - strand, within HMPREF1058_RS06245 at 528.237 kb on + strand, within HMPREF1058_RS06245 at 528.406 kb on + strand at 528.417 kb on + strand at 528.419 kb on + strand at 528.424 kb on - strand at 528.427 kb on - strand at 528.435 kb on + strand at 528.478 kb on + strand at 528.517 kb on + strand, within HMPREF1058_RS06240 at 528.591 kb on - strand, within HMPREF1058_RS06240 at 528.758 kb on + strand, within HMPREF1058_RS06240 at 528.759 kb on - strand, within HMPREF1058_RS06240 at 528.759 kb on - strand, within HMPREF1058_RS06240 at 528.827 kb on - strand, within HMPREF1058_RS06240 at 528.845 kb on + strand, within HMPREF1058_RS06240 at 528.845 kb on + strand, within HMPREF1058_RS06240 at 528.845 kb on + strand, within HMPREF1058_RS06240 at 528.846 kb on - strand, within HMPREF1058_RS06240 at 528.851 kb on - strand, within HMPREF1058_RS06240 at 528.851 kb on - strand, within HMPREF1058_RS06240 at 528.858 kb on + strand, within HMPREF1058_RS06240 at 528.859 kb on - strand, within HMPREF1058_RS06240 at 528.870 kb on + strand, within HMPREF1058_RS06240 at 528.871 kb on - strand, within HMPREF1058_RS06240 at 528.993 kb on + strand, within HMPREF1058_RS06240 at 528.993 kb on + strand, within HMPREF1058_RS06240 at 528.993 kb on + strand, within HMPREF1058_RS06240 at 528.993 kb on + strand, within HMPREF1058_RS06240 at 528.994 kb on - strand, within HMPREF1058_RS06240 at 528.994 kb on - strand, within HMPREF1058_RS06240 at 528.994 kb on - strand, within HMPREF1058_RS06240 at 529.109 kb on + strand at 529.587 kb on - strand at 529.607 kb on - strand at 529.666 kb on - strand at 529.689 kb on - strand at 529.778 kb on - strand at 529.780 kb on + strand at 529.780 kb on + strand at 529.812 kb on + strand at 529.812 kb on + strand at 529.818 kb on - strand at 529.818 kb on - strand at 529.976 kb on - strand, within HMPREF1058_RS06230 at 530.046 kb on + strand, within HMPREF1058_RS06230
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 527,479 - +1.1 527,479 - -0.6 527,589 + HMPREF1058_RS06245 0.15 -2.7 527,589 + HMPREF1058_RS06245 0.15 -0.8 527,590 - HMPREF1058_RS06245 0.15 -0.7 527,802 - HMPREF1058_RS06245 0.37 -0.5 527,802 - HMPREF1058_RS06245 0.37 +0.2 527,835 - HMPREF1058_RS06245 0.40 -0.2 527,965 + HMPREF1058_RS06245 0.54 +1.0 528,000 - HMPREF1058_RS06245 0.57 -0.5 528,168 + HMPREF1058_RS06245 0.74 +0.7 528,169 - HMPREF1058_RS06245 0.74 +1.6 528,193 + HMPREF1058_RS06245 0.77 -0.4 528,194 - HMPREF1058_RS06245 0.77 -0.0 528,194 - HMPREF1058_RS06245 0.77 +2.0 528,197 + HMPREF1058_RS06245 0.77 -0.0 528,197 + -0.6 528,197 + HMPREF1058_RS06245 0.77 -0.0 528,197 + HMPREF1058_RS06245 0.77 -0.9 528,197 + HMPREF1058_RS06245 0.77 +1.0 528,198 - HMPREF1058_RS06245 0.77 -0.5 528,198 - HMPREF1058_RS06245 0.77 +0.2 528,198 - HMPREF1058_RS06245 0.77 -0.6 528,198 - HMPREF1058_RS06245 0.77 -0.3 528,198 - HMPREF1058_RS06245 0.77 +1.7 528,198 - HMPREF1058_RS06245 0.77 +0.8 528,209 + HMPREF1058_RS06245 0.79 +1.5 528,233 + HMPREF1058_RS06245 0.81 +1.3 528,234 - HMPREF1058_RS06245 0.81 -1.2 528,237 + HMPREF1058_RS06245 0.81 +0.3 528,406 + -0.6 528,417 + -1.0 528,419 + +0.7 528,424 - -1.0 528,427 - -0.0 528,435 + +1.5 528,478 + +1.1 528,517 + HMPREF1058_RS06240 0.13 +1.5 528,591 - HMPREF1058_RS06240 0.24 -0.2 528,758 + HMPREF1058_RS06240 0.49 +2.5 528,759 - HMPREF1058_RS06240 0.49 +2.0 528,759 - HMPREF1058_RS06240 0.49 +0.9 528,827 - HMPREF1058_RS06240 0.59 +1.1 528,845 + HMPREF1058_RS06240 0.62 +0.5 528,845 + HMPREF1058_RS06240 0.62 +2.0 528,845 + HMPREF1058_RS06240 0.62 +2.1 528,846 - HMPREF1058_RS06240 0.62 +1.5 528,851 - HMPREF1058_RS06240 0.63 +1.4 528,851 - HMPREF1058_RS06240 0.63 +1.2 528,858 + HMPREF1058_RS06240 0.64 +2.7 528,859 - HMPREF1058_RS06240 0.64 -0.0 528,870 + HMPREF1058_RS06240 0.66 -0.2 528,871 - HMPREF1058_RS06240 0.66 +1.2 528,993 + HMPREF1058_RS06240 0.84 +0.5 528,993 + HMPREF1058_RS06240 0.84 +1.0 528,993 + HMPREF1058_RS06240 0.84 +2.9 528,993 + HMPREF1058_RS06240 0.84 +0.1 528,994 - HMPREF1058_RS06240 0.84 +0.4 528,994 - HMPREF1058_RS06240 0.84 +2.0 528,994 - HMPREF1058_RS06240 0.84 +0.0 529,109 + -0.3 529,587 - +1.5 529,607 - +0.5 529,666 - +1.0 529,689 - +0.7 529,778 - -0.3 529,780 + +0.2 529,780 + +1.2 529,812 + -0.3 529,812 + +1.9 529,818 - -0.5 529,818 - -2.6 529,976 - HMPREF1058_RS06230 0.21 -1.7 530,046 + HMPREF1058_RS06230 0.27 -0.6
Or see this region's nucleotide sequence