Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS05920 and HMPREF1058_RS05915 overlap by 10 nucleotides HMPREF1058_RS05915 and HMPREF1058_RS05910 are separated by 186 nucleotides HMPREF1058_RS05910 and HMPREF1058_RS0119820 are separated by 140 nucleotides HMPREF1058_RS0119820 and HMPREF1058_RS05905 are separated by 264 nucleotides
HMPREF1058_RS05920: HMPREF1058_RS05920 - hypothetical protein, at 48,958 to 49,524
_RS05920
HMPREF1058_RS05915: HMPREF1058_RS05915 - sigma-70 family RNA polymerase sigma factor, at 49,515 to 49,985
_RS05915
HMPREF1058_RS05910: HMPREF1058_RS05910 - galactose mutarotase, at 50,172 to 51,305
_RS05910
HMPREF1058_RS0119820: HMPREF1058_RS0119820 - hypothetical protein, at 51,446 to 51,675
_RS0119820
HMPREF1058_RS05905: HMPREF1058_RS05905 - sigma-70 family RNA polymerase sigma factor, at 51,940 to 52,485
_RS05905
Position (kb)
50
51
52 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 49.363 kb on - strand, within HMPREF1058_RS05920 at 49.363 kb on - strand, within HMPREF1058_RS05920 at 49.415 kb on - strand, within HMPREF1058_RS05920 at 49.415 kb on - strand, within HMPREF1058_RS05920 at 49.443 kb on + strand at 49.530 kb on - strand at 49.530 kb on - strand at 49.542 kb on - strand at 49.542 kb on - strand at 49.665 kb on - strand, within HMPREF1058_RS05915 at 49.692 kb on + strand, within HMPREF1058_RS05915 at 49.696 kb on - strand, within HMPREF1058_RS05915 at 49.715 kb on + strand, within HMPREF1058_RS05915 at 49.715 kb on + strand, within HMPREF1058_RS05915 at 50.040 kb on + strand at 50.041 kb on - strand at 50.046 kb on + strand at 50.104 kb on + strand at 50.124 kb on + strand at 50.125 kb on - strand at 50.125 kb on - strand at 50.126 kb on + strand at 50.126 kb on + strand at 50.126 kb on + strand at 50.127 kb on - strand at 50.127 kb on - strand at 50.127 kb on - strand at 50.129 kb on - strand at 50.183 kb on + strand at 50.183 kb on + strand at 50.192 kb on + strand at 50.192 kb on + strand at 50.229 kb on - strand at 50.229 kb on - strand at 50.229 kb on - strand at 50.307 kb on + strand, within HMPREF1058_RS05910 at 50.308 kb on - strand, within HMPREF1058_RS05910 at 50.318 kb on + strand, within HMPREF1058_RS05910 at 50.318 kb on + strand, within HMPREF1058_RS05910 at 50.413 kb on + strand, within HMPREF1058_RS05910 at 50.413 kb on + strand, within HMPREF1058_RS05910 at 50.414 kb on - strand, within HMPREF1058_RS05910 at 50.414 kb on - strand, within HMPREF1058_RS05910 at 50.436 kb on - strand, within HMPREF1058_RS05910 at 50.436 kb on - strand, within HMPREF1058_RS05910 at 50.436 kb on - strand, within HMPREF1058_RS05910 at 50.466 kb on - strand at 50.466 kb on - strand, within HMPREF1058_RS05910 at 50.474 kb on - strand, within HMPREF1058_RS05910 at 50.513 kb on + strand, within HMPREF1058_RS05910 at 50.514 kb on - strand, within HMPREF1058_RS05910 at 50.514 kb on - strand, within HMPREF1058_RS05910 at 50.595 kb on - strand, within HMPREF1058_RS05910 at 50.720 kb on + strand, within HMPREF1058_RS05910 at 50.721 kb on - strand, within HMPREF1058_RS05910 at 50.732 kb on - strand, within HMPREF1058_RS05910 at 50.770 kb on + strand, within HMPREF1058_RS05910 at 50.771 kb on - strand, within HMPREF1058_RS05910 at 50.771 kb on - strand, within HMPREF1058_RS05910 at 50.843 kb on + strand, within HMPREF1058_RS05910 at 50.844 kb on - strand, within HMPREF1058_RS05910 at 50.844 kb on - strand, within HMPREF1058_RS05910 at 50.850 kb on - strand, within HMPREF1058_RS05910 at 50.907 kb on + strand, within HMPREF1058_RS05910 at 50.907 kb on + strand, within HMPREF1058_RS05910 at 50.908 kb on - strand, within HMPREF1058_RS05910 at 50.909 kb on + strand, within HMPREF1058_RS05910 at 50.910 kb on - strand, within HMPREF1058_RS05910 at 50.942 kb on + strand, within HMPREF1058_RS05910 at 50.942 kb on + strand, within HMPREF1058_RS05910 at 50.950 kb on - strand, within HMPREF1058_RS05910 at 50.989 kb on - strand, within HMPREF1058_RS05910 at 50.989 kb on - strand, within HMPREF1058_RS05910 at 51.032 kb on + strand, within HMPREF1058_RS05910 at 51.034 kb on + strand, within HMPREF1058_RS05910 at 51.034 kb on + strand, within HMPREF1058_RS05910 at 51.034 kb on + strand, within HMPREF1058_RS05910 at 51.101 kb on + strand, within HMPREF1058_RS05910 at 51.102 kb on - strand, within HMPREF1058_RS05910 at 51.122 kb on - strand, within HMPREF1058_RS05910 at 51.306 kb on - strand at 51.326 kb on + strand at 51.342 kb on - strand at 51.486 kb on + strand, within HMPREF1058_RS0119820 at 51.563 kb on - strand, within HMPREF1058_RS0119820 at 51.601 kb on + strand, within HMPREF1058_RS0119820 at 51.602 kb on - strand, within HMPREF1058_RS0119820 at 51.675 kb on + strand at 51.722 kb on + strand at 51.827 kb on + strand at 51.844 kb on + strand at 51.845 kb on - strand at 51.862 kb on - strand at 51.862 kb on - strand at 51.909 kb on + strand at 51.909 kb on + strand at 51.910 kb on - strand at 51.957 kb on - strand at 52.001 kb on + strand, within HMPREF1058_RS05905 at 52.003 kb on + strand, within HMPREF1058_RS05905 at 52.003 kb on + strand, within HMPREF1058_RS05905 at 52.004 kb on - strand, within HMPREF1058_RS05905 at 52.028 kb on - strand, within HMPREF1058_RS05905 at 52.046 kb on - strand, within HMPREF1058_RS05905 at 52.267 kb on - strand, within HMPREF1058_RS05905
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 49,363 - HMPREF1058_RS05920 0.71 +1.1 49,363 - HMPREF1058_RS05920 0.71 +1.4 49,415 - HMPREF1058_RS05920 0.81 +1.4 49,415 - HMPREF1058_RS05920 0.81 +1.4 49,443 + +0.1 49,530 - +2.0 49,530 - -0.6 49,542 - -1.7 49,542 - -0.4 49,665 - HMPREF1058_RS05915 0.32 -1.7 49,692 + HMPREF1058_RS05915 0.38 -1.1 49,696 - HMPREF1058_RS05915 0.38 +0.1 49,715 + HMPREF1058_RS05915 0.42 +3.1 49,715 + HMPREF1058_RS05915 0.42 -0.4 50,040 + +0.4 50,041 - +0.9 50,046 + -1.4 50,104 + +0.7 50,124 + -0.4 50,125 - -2.3 50,125 - +0.4 50,126 + +0.2 50,126 + +1.0 50,126 + +0.3 50,127 - -1.2 50,127 - -0.4 50,127 - -1.0 50,129 - +0.9 50,183 + +0.5 50,183 + -2.5 50,192 + +0.2 50,192 + -0.2 50,229 - +0.9 50,229 - -1.8 50,229 - +0.1 50,307 + HMPREF1058_RS05910 0.12 -1.7 50,308 - HMPREF1058_RS05910 0.12 +0.1 50,318 + HMPREF1058_RS05910 0.13 -1.1 50,318 + HMPREF1058_RS05910 0.13 -2.2 50,413 + HMPREF1058_RS05910 0.21 -1.3 50,413 + HMPREF1058_RS05910 0.21 +0.9 50,414 - HMPREF1058_RS05910 0.21 -2.0 50,414 - HMPREF1058_RS05910 0.21 -2.5 50,436 - HMPREF1058_RS05910 0.23 -0.7 50,436 - HMPREF1058_RS05910 0.23 -0.6 50,436 - HMPREF1058_RS05910 0.23 +1.1 50,466 - +0.9 50,466 - HMPREF1058_RS05910 0.26 +0.2 50,474 - HMPREF1058_RS05910 0.27 +0.9 50,513 + HMPREF1058_RS05910 0.30 +0.0 50,514 - HMPREF1058_RS05910 0.30 +1.0 50,514 - HMPREF1058_RS05910 0.30 -2.9 50,595 - HMPREF1058_RS05910 0.37 +0.4 50,720 + HMPREF1058_RS05910 0.48 -0.5 50,721 - HMPREF1058_RS05910 0.48 -0.1 50,732 - HMPREF1058_RS05910 0.49 -2.0 50,770 + HMPREF1058_RS05910 0.53 +1.1 50,771 - HMPREF1058_RS05910 0.53 +0.3 50,771 - HMPREF1058_RS05910 0.53 +0.1 50,843 + HMPREF1058_RS05910 0.59 +0.3 50,844 - HMPREF1058_RS05910 0.59 -1.6 50,844 - HMPREF1058_RS05910 0.59 -0.6 50,850 - HMPREF1058_RS05910 0.60 -0.4 50,907 + HMPREF1058_RS05910 0.65 -1.0 50,907 + HMPREF1058_RS05910 0.65 -0.4 50,908 - HMPREF1058_RS05910 0.65 -1.5 50,909 + HMPREF1058_RS05910 0.65 -2.0 50,910 - HMPREF1058_RS05910 0.65 +0.1 50,942 + HMPREF1058_RS05910 0.68 -1.0 50,942 + HMPREF1058_RS05910 0.68 -2.7 50,950 - HMPREF1058_RS05910 0.69 +1.4 50,989 - HMPREF1058_RS05910 0.72 -0.9 50,989 - HMPREF1058_RS05910 0.72 +2.4 51,032 + HMPREF1058_RS05910 0.76 +1.3 51,034 + HMPREF1058_RS05910 0.76 -1.1 51,034 + HMPREF1058_RS05910 0.76 -0.3 51,034 + HMPREF1058_RS05910 0.76 +1.6 51,101 + HMPREF1058_RS05910 0.82 +2.3 51,102 - HMPREF1058_RS05910 0.82 +1.2 51,122 - HMPREF1058_RS05910 0.84 +1.0 51,306 - +0.6 51,326 + -0.1 51,342 - -0.4 51,486 + HMPREF1058_RS0119820 0.17 +0.3 51,563 - HMPREF1058_RS0119820 0.51 +1.1 51,601 + HMPREF1058_RS0119820 0.67 -0.6 51,602 - HMPREF1058_RS0119820 0.68 -3.1 51,675 + -2.0 51,722 + +2.1 51,827 + -2.7 51,844 + -2.0 51,845 - -0.0 51,862 - +0.9 51,862 - -3.0 51,909 + -0.0 51,909 + -1.4 51,910 - -1.0 51,957 - -0.4 52,001 + HMPREF1058_RS05905 0.11 -1.1 52,003 + HMPREF1058_RS05905 0.12 -0.6 52,003 + HMPREF1058_RS05905 0.12 -2.3 52,004 - HMPREF1058_RS05905 0.12 -2.1 52,028 - HMPREF1058_RS05905 0.16 +1.0 52,046 - HMPREF1058_RS05905 0.19 +0.3 52,267 - HMPREF1058_RS05905 0.60 -2.3
Or see this region's nucleotide sequence